Abstract:
:Infection of a murine-spleen dendritic cell line by Listeria monocytogenes was found to induce cell death through apoptosis. To characterize the bacterial product(s) involved in induction of apoptosis, dendritic cells were infected with the L. monocytogenes EGD strain and several isogenic mutants deficient in the production of individual listerial virulence factors. The ability to induce cellular apoptosis was retained by all mutants tested, except the prfA and delta hly mutants, both of which are unable to produce listeriolysin. Apoptosis was also induced by purified listeriolysin suggesting that this protein directly induces apoptosis. Purified recombinant listeriolysins rendered either weakly haemolytic by a C-484 to S mutation, or nonhaemolytic by a W-491 to A mutation exhibited little or no capacity to induce apoptosis, indicating that both activities are associated within the same protein region. Treatment with purified listeriolysin or L. monocytogenes infection also triggers apoptosis in explanted bone-marrow dendritic cells. Thus invasion of dendritic cells by L. monocytogenes, which results in cell death, may play an important role in the pathogenesis of listerial infections by impairing immune responses, hindering bacterial clearance and promoting spread of the infection.
journal_name
Mol Microbioljournal_title
Molecular microbiologyauthors
Guzmán CA,Domann E,Rohde M,Bruder D,Darji A,Weiss S,Wehland J,Chakraborty T,Timmis KNdoi
10.1111/j.1365-2958.1996.tb02494.xsubject
Has Abstractpub_date
1996-04-01 00:00:00pages
119-26issue
1eissn
0950-382Xissn
1365-2958journal_volume
20pub_type
杂志文章abstract::The pulmonary pathogen Legionella pneumophila uses the Dot/Icm type IV secretion system (T4SS) to replicate inside host cells. This apparatus translocates proteins into macrophages to alter their endocytic pathway and enable bacterial growth. Although the secretion ATPase DotB is critical for T4SS function, its specif...
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journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:1996-09-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
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journal_title:Molecular microbiology
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pub_type: 杂志文章
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pub_type: 杂志文章,评审
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journal_title:Molecular microbiology
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更新日期:1997-05-01 00:00:00
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更新日期:2014-01-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:1995-12-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:2015-01-01 00:00:00
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