Abstract:
:The formation of biofilm results in a major lifestyle switch that is thought to affect the expression of multiple genes and operons. We used DNA arrays to study the global effect of biofilm formation on gene expression in mature Escherichia coli K-12 biofilm. We show that, when biofilm is compared with the exponential growth phase, 1.9% of the genes showed a consistent up- or downregulation by a factor greater than two, and that 10% of the E. coli genome is significantly differentially expressed. The functions of the genes induced in these conditions correspond to stress response as well as energy production, envelope biogenesis and unknown functions. We provide evidence that the expression of stress envelope response genes, such as the psp operon or elements of the cpx and rpoE pathways, is a general feature of E. coli mature biofilms. We also compared biofilm with the stationary growth phase and showed that the biofilm lifestyle, although sharing similarities with the stationary growth phase, triggers the expression of specific sets of genes. Using gene disruption of 54 of the most biofilm-induced genes followed by a detailed phenotypic study, we validated the biological relevance of our analysis and showed that 20 of these genes are required for the formation of mature biofilm. This group includes 11 genes of previously unknown function. These results constitute a comprehensive analysis of the global transcriptional response triggered in mature E. coli biofilms and provide insights into its physiological signature.
journal_name
Mol Microbioljournal_title
Molecular microbiologyauthors
Beloin C,Valle J,Latour-Lambert P,Faure P,Kzreminski M,Balestrino D,Haagensen JA,Molin S,Prensier G,Arbeille B,Ghigo JMdoi
10.1046/j.1365-2958.2003.03865.xsubject
Has Abstractpub_date
2004-02-01 00:00:00pages
659-74issue
3eissn
0950-382Xissn
1365-2958journal_volume
51pub_type
杂志文章abstract::ICEMlSym(R7A) of Mesorhizobium loti is an integrative and conjugative element (ICE) that confers the ability to form a nitrogen-fixing symbiosis with Lotus species. Horizontal transfer is activated by TraR and N-acyl-homoserine lactone (AHL), which can stimulate ICE excision in 100% of cells. However, in wild-type cu...
journal_title:Molecular microbiology
pub_type: 杂志文章
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journal_title:Molecular microbiology
pub_type: 杂志文章
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2958.1996.d01-1712.x
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journal_title:Molecular microbiology
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journal_title:Molecular microbiology
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journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:2012-10-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2008-02-01 00:00:00
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pub_type: 杂志文章
doi:10.1046/j.1365-2958.2000.02083.x
更新日期:2000-09-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2958.1996.393934.x
更新日期:1996-02-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2958.2008.06515.x
更新日期:2009-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:1992-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:2010-09-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2958.2011.07869.x
更新日期:2011-11-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2958.1995.mmi_18030579.x
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pub_type: 杂志文章,评审
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2958.2002.02868.x
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pub_type: 杂志文章
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journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:2013-08-01 00:00:00
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journal_title:Molecular microbiology
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pub_type: 评论,杂志文章,评审
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更新日期:2005-08-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章,评审
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journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:2020-09-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章,评审
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更新日期:2000-08-01 00:00:00