Off-pathway assembly of fimbria subunits is prevented by chaperone CfaA of CFA/I fimbriae from enterotoxigenic E. coli.

abstract：:Fungi capable of sexual reproduction use heterothallic (self-sterile) or homothallic (self-fertile) mating strategies. In most ascomycetes, a single mating type locus, MAT, with two alternative forms (MAT1-1 and MAT1-2) called idiomorphs, controls mating ability. In heterothallic ascomycetes, these alternative idiomor...

journal_title：Molecular microbiology

authors： Lee J,Lee T,Lee YW,Yun SH,Turgeon BG

更新日期：2003-10-01 00:00:00

abstract：:The recombination of short homologous ends in Escherichia coli has been known for 30 years, and it is often used for both site-directed mutagenesis and in vivo cloning. For cloning, a plasmid and target DNA fragments were converted into linear DNA fragments with short homologous ends, which are joined via recombinatio...

journal_title：Molecular microbiology

authors： Yang Y,Wang T,Yu Q,Liu H,Xun L,Xia Y

更新日期：2020-12-29 00:00:00

abstract：:The majority of bacterial genomes encode a high number of two-component systems controlling gene expression in response to a variety of different stimuli. The Gram-positive soil bacterium Corynebacterium glutamicum contains two homologous two-component systems (TCS) involved in the haem-dependent regulation of gene ex...

journal_title：Molecular microbiology

authors： Hentschel E,Mack C,Gätgens C,Bott M,Brocker M,Frunzke J

更新日期：2014-06-01 00:00:00

abstract：:The c-di-GMP-binding effector protein FlgZ has been demonstrated to control motility in the opportunistic pathogen Pseudomonas aeruginosa and it was suggested that c-di-GMP-bound FlgZ impedes motility via its interaction with the MotCD stator. To further understand how motility is downregulated in P. aeruginosa and to...

journal_title：Molecular microbiology

authors： Bense S,Bruchmann S,Steffen A,Stradal TEB,Häussler S,Düvel J

更新日期：2019-06-01 00:00:00

abstract：:Through trans-splicing of a 39-nt spliced leader (SL) onto each protein-coding transcript, mature kinetoplastid mRNA acquire a hypermethylated 5'-cap structure, but its function has been unclear. Gene deletions for three Trypanosoma brucei cap 2'-O-ribose methyltransferases, TbMTr1, TbMTr2 and TbMTr3, reveal distinct ...

journal_title：Molecular microbiology

authors： Zamudio JR,Mittra B,Campbell DA,Sturm NR

更新日期：2009-06-01 00:00:00

abstract：:The best studied role of ubiquitination is to mark proteins for destruction by the proteasome but, in addition, it has recently been shown to promote macromolecular assembly and function, and alter protein function, thus playing a regulatory role distinct from protein degradation. Deubiquinating enzymes, the ubiquitin...

journal_title：Molecular microbiology

authors： Lockington RA,Kelly JM

更新日期：2001-06-01 00:00:00

abstract：:We have followed the time-dependent transfection of Paramecium cells with a vector containing the gene of green fluorescent protein (GFP) attached to the C-terminus of the PtSERCA1 gene. The outlines of alveolar sacs (ASs) are labelled, as is the endoplasmic reticulum (ER) throughout the cell. When GFP fluorescence is...

journal_title：Molecular microbiology

authors： Hauser K,Pavlovic N,Klauke N,Geissinger D,Plattner H

更新日期：2000-08-01 00:00:00

abstract：:Cell morphogenesis is a complex process that depends on cytoskeleton and membrane organization, intracellular signalling and vesicular trafficking. The rod shape of the fission yeast Schizosaccharomyces pombe and the availability of powerful genetic tools make this species an excellent model to study cell morphology. ...

journal_title：Molecular microbiology

authors： Cadou A,Couturier A,Le Goff C,Soto T,Miklos I,Sipiczki M,Xie L,Paulson JR,Cansado J,Le Goff X

更新日期：2010-09-01 00:00:00

abstract：:Pseudomonas aeruginosa chronically colonizing the lungs of cystic fibrosis (CF) patients undergoes fast evolution leading to clonal divergence. More than half of the genotypes of P. aeruginosa clone C isolates exclusively from CF lung infection exhibit large chromosomal inversions (LCIs). To analyse the impact of LCIs...

journal_title：Molecular microbiology

authors： Kresse AU,Dinesh SD,Larbig K,Römling U

更新日期：2003-01-01 00:00:00

abstract：:We have found a simple relationship between bacterial growth rate and the translation elongation rate. Thus, for a set of defined ribosomal protein S12 mutations which reduce the efficiency of the ternary complex ribosome interaction (and restrict the frequency of translational errors) there is a linear relationship b...

journal_title：Molecular microbiology

authors： Tubulekas I,Hughes D

更新日期：1993-01-01 00:00:00

abstract：:Alternative sigma (σ) factors govern expression of bacterial genes in response to diverse environmental signals. In Pseudomonas aeruginosa σPvdS directs expression of genes for production of a siderophore, pyoverdine, as well as a toxin and a protease. σFpvI directs expression of a receptor for ferripyoverdine import....

journal_title：Molecular microbiology

authors： Edgar RJ,Hampton GE,Garcia GPC,Maher MJ,Perugini MA,Ackerley DF,Lamont IL

更新日期：2017-12-01 00:00:00

abstract：:The flow of information from the outside to the inside of bacterial cells is largely directed by protein kinases. In addition to histidine/aspartate phosphorelays of two-component response regulators, recent work in Mycobacterium tuberculosis (Mtb) reinforces the idea that phosphorylation on serine (Ser), threonine (T...

journal_title：Molecular microbiology

authors： Sherman DR,Grundner C

更新日期：2014-10-01 00:00:00

abstract：:FlhD and FlhC are the transcriptional activators of the flagellar regulon. The heterotetrameric complex formed by these two proteins activates the transcription of the class II flagellar genes. The flagellar regulon consists not only of flagellar genes, but also of the chemotactic genes and some receptor proteins. Rec...

journal_title：Molecular microbiology

authors： Campos A,Matsumura P

更新日期：2001-02-01 00:00:00

abstract：:Type IV pili (T4P) are surface structures that undergo extension/retraction oscillations to generate cell motility. In Myxococcus xanthus, T4P are unipolarly localized and undergo pole-to-pole oscillations synchronously with cellular reversals. We investigated the mechanisms underlying these oscillations. We show that...

journal_title：Molecular microbiology

authors： Bulyha I,Schmidt C,Lenz P,Jakovljevic V,Höne A,Maier B,Hoppert M,Søgaard-Andersen L

更新日期：2009-11-01 00:00:00

abstract：:The ArsAB extrusion pump encoded by the ars operon of Escherichia coli plasmid R773 confers resistance to the toxic trivalent metalloids arsenite [As(III)] and antimonite [Sb(III)]. The ArsA ATPase, the catalytic subunit of the pump, has two homologous halves, A1 and A2. At the interface of these two halves are two nu...

journal_title：Molecular microbiology

authors： Ruan X,Bhattacharjee H,Rosen BP

更新日期：2008-01-01 00:00:00

abstract：:Transcript degradation is a key step in gene regulation. In eukaryotes, mRNA decay is generally initiated by removal of the poly(A) tail mediated by the Ccr4-Caf1-Not complex. Deadenylated transcripts are then rapidly degraded, primarily via the decapping-dependent pathway. Components of this pathway can be localized ...

journal_title：Molecular microbiology

authors： Morozov IY,Jones MG,Spiller DG,Rigden DJ,Dattenböck C,Novotny R,Strauss J,Caddick MX

更新日期：2010-04-01 00:00:00

abstract：:The carboxyl-terminal region of diphtheria toxin (DT) has been analysed in order to determine regions of receptor recognition. Biochemical cleavage of the toxin with hydroxylamine (HA) was used to generate the peptides HA9DT (residues 454-535), HA6DT (residues 482-535), and HA3DT (residues 454-481). Characterization o...

journal_title：Molecular microbiology

authors： Rolf JM,Eidels L

更新日期：1993-02-01 00:00:00

abstract：:Choline-binding proteins (CBPs) from Streptococcus pneumoniae are involved in several important processes. Inactivation of zmpB, a gene that encodes a surface-located putative zinc metalloprotease, in a S. pneumoniae serotype 4 strain was recently reported to reveal a composite phenotype, including extensive chain for...

journal_title：Molecular microbiology

authors： Bergé M,García P,Iannelli F,Prère MF,Granadel C,Polissi A,Claverys JP

更新日期：2001-03-01 00:00:00

abstract：:Many fundamental cellular processes depend on enzymes that utilize chemical energy to catalyse unfavourable reactions. Certain classes of ATPases provide a particularly vivid example of the process of energy conversion, employing cycles of nucleotide turnover to move and/or rearrange biological polymers such as protei...

journal_title：Molecular microbiology

authors： Thomsen ND,Berger JM

更新日期：2008-09-01 00:00:00

abstract：:Borrelia burgdorferi, the Lyme disease agent, has double-stranded linear plasmids with covalently closed ends. DNA at the ends, or telomeres, of two linear plasmids of B. burgdorferi strain B31 was examined. Telomeric sequences from both ends of a 16 kb linear plasmid and from one end of a 49 kb linear plasmid were cl...

journal_title：Molecular microbiology

authors： Hinnebusch J,Bergström S,Barbour AG

更新日期：1990-05-01 00:00:00

abstract：:The lipoprotein Lpp is the most numerically abundant protein in Escherichia coli, has been investigated for over 40 years, and has served as the paradigmatic bacterial lipoprotein since its initial discovery. It exists in two distinct forms: a 'bound-form', which is covalently bound to the cell's peptidoglycan layer, ...

journal_title：Molecular microbiology

authors： Cowles CE,Li Y,Semmelhack MF,Cristea IM,Silhavy TJ

更新日期：2011-03-01 00:00:00

abstract：:The presence of very different sets of enzymes, and in particular the presence of RNase E and RNase J, has been used to explain significant differences in RNA metabolism between the two model organisms Escherichia coli and Bacillus subtilis. However, these studies might have somewhat polarized our view of RNA metaboli...

journal_title：Molecular microbiology

authors： Taverniti V,Forti F,Ghisotti D,Putzer H

更新日期：2011-12-01 00:00:00

abstract：:Elevated expression of the plasma membrane drug efflux pump proteins Cdr1p and Cdr2p was shown to accompany decreased azole susceptibility in Candida albicans clinical isolates. DNA sequence analysis revealed extensive allelic heterozygosity, particularly of CDR2. Cdr2p alleles showed different abilities to transport ...

journal_title：Molecular microbiology

authors： Holmes AR,Tsao S,Ong SW,Lamping E,Niimi K,Monk BC,Niimi M,Kaneko A,Holland BR,Schmid J,Cannon RD

更新日期：2006-10-01 00:00:00

abstract：:The expression of dnaA is autoregulated, in that transcription of the gene increases when DnaA is inactivated (and initiation of replication prevented) and decreases when DnaA is supplied in excess. However, the inactivation of DnaA does not necessarily lead to increased DnaA production, as dnaA(Ts; temperature sensit...

journal_title：Molecular microbiology

authors： Smith RW,McAteer S,Masters M

更新日期：1997-03-01 00:00:00

abstract：:Legionella pneumophila, the causative agent of Legionnaires' disease, survives in macrophages by altering the endocytic pathway of its host cell. To accomplish this, the bacterium utilizes a type IVB secretion system to deliver effector molecules into the host cell cytoplasm. In a previous report, we performed an exte...

journal_title：Molecular microbiology

authors： Vincent CD,Friedman JR,Jeong KC,Sutherland MC,Vogel JP

更新日期：2012-07-01 00:00:00

abstract：:During Bacillus subtilis endospore formation, a complex protein coat is assembled around the maturing spore. The coat is made up of more than two dozen proteins that form an outer layer, which provides chemical resistance, and an inner layer, which may play a role in the activation of germination. A third, amorphous l...

journal_title：Molecular microbiology

authors： Seyler RW Jr,Henriques AO,Ozin AJ,Moran CP Jr

更新日期：1997-09-01 00:00:00

abstract：:Encased within the 280 kb genome in the capsid of the giant myovirus φKZ is an unusual cylindrical proteinaceous 'inner body' of highly ordered structure. We present here mass spectrometry, bioinformatic and biochemical studies that reveal novel information about the φKZ head and the complex inner body. The identifica...

journal_title：Molecular microbiology

authors： Thomas JA,Weintraub ST,Wu W,Winkler DC,Cheng N,Steven AC,Black LW

更新日期：2012-04-01 00:00:00

abstract：:The sensor kinase/response regulator system KdpD/KdpE of Escherichia coli regulates the expression of the kdpFABC operon, which encodes the high affinity K+ transport system KdpFABC. The membrane-bound sensor kinase KdpD consists of four transmembrane domains, a large cytoplasmic N-terminal domain and a cytoplasmic C-...

journal_title：Molecular microbiology

authors： Heermann R,Fohrmann A,Altendorf K,Jung K

更新日期：2003-02-01 00:00:00

abstract：:Formation of the asymmetrically located septum divides sporulating bacilli into two distinct cells: the mother cell and the prespore. The rigidifying wall material in the septum is subsequently removed by autolysis. Examination of published electron micrographs indicates that the two septal membranes then fuse to form...

journal_title：Molecular microbiology

authors： Higgins ML,Piggot PJ

更新日期：1992-09-01 00:00:00

abstract：:Streptococcus mutans, a commensal of the human oral cavity, can survive in the bloodstream and cause infective endocarditis (IE). However, the virulence factors associated with this manifestation of disease are not known. Here, we demonstrate that AtlA, an autolysin of S. mutans is a newly identified fibronectin (Fn) ...

journal_title：Molecular microbiology

authors： Jung CJ,Zheng QH,Shieh YH,Lin CS,Chia JS

更新日期：2009-11-01 00:00:00