Autoregulation of the Escherichia coli replication initiator protein, DnaA, is indirect.

abstract：:Expression of Escherichia coli hupA and hupB, the structural genes encoding the most abundant nucleoid-associated proteins HUalpha and HUbeta has been studied during cold shock. This article demonstrates that: (i) transcriptional expression of hupA is blocked following a sudden temperature downshift (from 37 degrees C...

journal_title：Molecular microbiology

authors： Giangrossi M,Giuliodori AM,Gualerzi CO,Pon CL

更新日期：2002-04-01 00:00:00

abstract：:The FNR protein in Escherichia coli is an O(2) sensor that modifies global gene expression to adapt the cell to anaerobic growth. Regulation of FNR involves continuous cycling of the protein between its active and inactive states under aerobic conditions without apparent function. This raises the question of what bene...

journal_title：Molecular microbiology

authors： Tolla DA,Savageau MA

更新日期：2011-01-01 00:00:00

abstract：:Type IV secretion systems (T4SS) are utilized by a wide range of Gram negative bacteria to deliver protein and DNA substrates to recipient cells. The best characterized T4SS are the type IVA systems, which exhibit extensive similarity to the Agrobacterium VirB T4SS. In contrast, type IVB secretion systems share almost...

journal_title：Molecular microbiology

authors： Vincent CD,Friedman JR,Jeong KC,Buford EC,Miller JL,Vogel JP

更新日期：2006-12-01 00:00:00

abstract：:Polycyclic aromatic hydrocarbons (PAHs) are persistent and toxic environmental pollutants that accumulate in anoxic habitats. With the exception of naphthalene, nothing is known about the microbial degradation of PAH in these environments. The challenge that must be met in anaerobic PAH degradation is the destabilizat...

journal_title：Molecular microbiology

authors： Sawers RG

更新日期：2015-01-01 00:00:00

abstract：:A simplified system using bacterial insertion sequence IS911 has been developed to investigate targeted insertion next to DNA sequences resembling IS ends. We show here that these IR-targeted events occur by an unusual mechanism. In the circular IS911 transposition intermediate the two IRs are abutted to form an IR/IR...

journal_title：Molecular microbiology

authors： Loot C,Turlan C,Chandler M

更新日期：2004-01-01 00:00:00

abstract：:srfA is a locus required for the production of the lipopeptide antibiotic surfactin. This locus is also necessary for efficient sporulation and competence development. Mutations in the 5' portion of the srfA operon affect all three of these processes, whereas mutations in the 3' portion of srfA only affect sporulation...

journal_title：Molecular microbiology

authors： van Sinderen D,Galli G,Cosmina P,de Ferra F,Withoff S,Venema G,Grandi G

更新日期：1993-05-01 00:00:00

abstract：:The genome of the malaria parasite Plasmodium falciparum contains several multicopy gene families, including var, rifin, stevor and Pfmc-2TM. These gene families undergo expression switching and appear to play a role in antigenic variation. It has recently been shown that forcing parasites to express high copy numbers...

journal_title：Molecular microbiology

authors： Howitt CA,Wilinski D,Llinás M,Templeton TJ,Dzikowski R,Deitsch KW

更新日期：2009-09-01 00:00:00

abstract：:Lactose is an abundant dietary carbohydrate metabolized by the dental pathogen Streptococcus mutans. Lactose metabolism presents both classic diauxic behaviors and long-term memory, where the bacteria can pause for >11 h before initiating growth on lactose. Here, we explored mechanisms contributing to unusual aspects ...

journal_title：Molecular microbiology

authors： Zeng L,Burne RA

更新日期：2020-09-03 00:00:00

abstract：:The diffusible factor synthase XanB2, originally identified in Xanthomonas campestris pv. campestris (Xcc), is highly conserved across a wide range of bacterial species, but its substrate and catalytic mechanism have not yet been investigated. Here, we show that XanB2 is a unique bifunctional chorismatase that hydroly...

journal_title：Molecular microbiology

authors： Zhou L,Wang JY,Wu J,Wang J,Poplawsky A,Lin S,Zhu B,Chang C,Zhou T,Zhang LH,He YW

更新日期：2013-01-01 00:00:00

abstract：:The relF gene in Escherichia coli is related to the hok gene on plasmid R1. Both genes encode small proteins which, when overexpressed in E. coli lead to collapse of the membrane potential and cell death. A third gene, designated gef, which encodes a homologous cell-toxic protein, has been isolated from E. coli DNA. B...

journal_title：Molecular microbiology

authors： Poulsen LK,Larsen NW,Molin S,Andersson P

更新日期：1989-11-01 00:00:00

abstract：:Among enteric bacteria, the ability to grow on N-acetyl-galactosamine (GalNAc or Aga) and on D-galactosamine (GalN or Gam) differs. Thus, strains B, C and EC3132 of Escherichia coli are Aga+ Gam+ whereas E. coli K-12 is Aga- Gam-, similarly to Klebsiella pneumoniae KAY2026, Klebsiella oxytoca M5a1 and Salmonella typhi...

journal_title：Molecular microbiology

authors： Brinkkötter A,Klöss H,Alpert C,Lengeler JW

更新日期：2000-07-01 00:00:00

abstract：:Sinorhizobium meliloti and host legumes enter into a nitrogen-fixing, symbiotic relationship triggered by an exchange of signals between bacteria and plant. S. meliloti produces Nod factor, which elicits the formation of nodules on plant roots, and succinoglycan, an exopolysaccharide that allows for bacterial invasion...

journal_title：Molecular microbiology

authors： Wells DH,Long SR

更新日期：2002-03-01 00:00:00

abstract：:GspB is a large cell-surface glycoprotein expressed by Streptococcus gordonii M99 that mediates binding of this organism to human platelets. This adhesin is glycosylated in the cytoplasm, and is then transported to the cell surface via an accessory Sec system. To assess the structural features of GspB that are needed ...

journal_title：Molecular microbiology

authors： Bensing BA,Takamatsu D,Sullam PM

更新日期：2005-12-01 00:00:00

abstract：:The malaria parasite Plasmodium falciparum is highly adapted to cope with the oxidative stress to which it is exposed during the erythrocytic stages of its life cycle. This includes the defence against oxidative insults arising from the parasite's metabolism of haemoglobin which results in the formation of reactive ox...

journal_title：Molecular microbiology

authors： Müller S

更新日期：2004-09-01 00:00:00

abstract：:DnaA protein (a trans-acting element) and its binding sequence, DnaA-box: (a cis-acting element) are two elements essential for the initiation of chromosomal replication in Escherichia coli and other enteric bacteria. Recently these two elements have been found to be conserved in three Gram-positive bacteria (Bacillus...

journal_title：Molecular microbiology

authors： Yoshikawa H,Ogasawara N

更新日期：1991-11-01 00:00:00

abstract：:Vibrio cholerae, the causative agent of the acute diarrheal disease cholera, is able to thrive in diverse habitats such as natural water bodies and inside human hosts. To ensure their survival, these bacteria rely on chemosensory pathways to sense and respond to changing environmental conditions. These pathways consti...

journal_title：Molecular microbiology

authors： Ortega DR,Kjaer A,Briegel A

更新日期：2020-09-01 00:00:00

abstract：:Bacterial cytokinesis requires the divisome, a complex of proteins that co-ordinates the invagination of the cytoplasmic membrane, inward growth of the peptidoglycan layer and the outer membrane. Assembly of the cell division proteins is tightly regulated and the order of appearance at the future division site is well...

journal_title：Molecular microbiology

authors： van den Ent F,Vinkenvleugel TM,Ind A,West P,Veprintsev D,Nanninga N,den Blaauwen T,Löwe J

更新日期：2008-04-01 00:00:00

abstract：:NsrR is a nitric oxide (NO)-sensitive transcription repressor that controls NO metabolism in a wide range of bacteria. In Bacillus subtilis, NsrR represses transcription of the nitrite reductase (nasDEF) genes that are under positive control of the ResD-ResE two-component signal transduction system. Derepression is ac...

journal_title：Molecular microbiology

authors： Kommineni S,Yukl E,Hayashi T,Delepine J,Geng H,Moënne-Loccoz P,Nakano MM

更新日期：2010-12-01 00:00:00

abstract：:The NarL and NarP proteins are homologous response regulators of Escherichia coli that control the expression of several operons in response to nitrate and nitrite. A consensus heptameric NarL DNA-binding sequence has been identified, and previous observations suggest that the NarP protein has a similar sequence speci...

journal_title：Molecular microbiology

authors： Darwin AJ,Tyson KL,Busby SJ,Stewart V

更新日期：1997-08-01 00:00:00

abstract：:A mechanistic hypothesis for the origin of the three domains of life is proposed. A population of evolving pre-cells is suggested to have had a membrane of a racemate of chiral lipids that continuously underwent spontaneous symmetry breaking by spatial phase segregation into two enantiomerically enriched membrane doma...

journal_title：Molecular microbiology

authors： Wächtershäuser G

更新日期：2003-01-01 00:00:00

abstract：:Restriction and modification (R-M) systems are generally thought to protect bacteria from invasion by foreign DNA. This paper proposes the existence of an alternative role for the phase-variable R-M systems encoded by the hsd loci of Mycoplasma pulmonis. Populations of M. pulmonis cells that arose during growth in dif...

journal_title：Molecular microbiology

authors： Gumulak-Smith J,Teachman A,Tu AH,Simecka JW,Lindsey JR,Dybvig K

更新日期：2001-05-01 00:00:00

abstract：:Comparison of the Leishmania infantum genome with Leishmania braziliensis and Leishmania major genomes has identified 25 L. infantum species-specific genes that are absent or pseudogenes in L. major and L. braziliensis. To determine whether these L. infantum species-specific genes are involved in visceral Leishmania i...

journal_title：Molecular microbiology

authors： Zhang WW,Matlashewski G

更新日期：2010-07-01 00:00:00

abstract：:The Escherichia coli fructose repressor, FruR, is known to regulate expression of several genes concerned with carbon utilization. Using a previously derived consensus sequence for FruR binding, additional potential operators were identified and tested for FruR binding in DNA band migration retardation assays. Operato...

journal_title：Molecular microbiology

authors： Ramseier TM,Bledig S,Michotey V,Feghali R,Saier MH Jr

更新日期：1995-06-01 00:00:00

abstract：:Iron-sulfur (Fe-S) clusters are prosthetic groups on proteins that function in a range of enzymatic and electron transfer reactions. Fe-S cluster synthesis is essential for the survival of all eukaryotes. Independent Fe-S cluster biosynthesis pathways occur in the mitochondrion, plastid, and cytosolic compartments of ...

journal_title：Molecular microbiology

authors： Aw YTV,Seidi A,Hayward JA,Lee J,Makota FV,Rug M,van Dooren GG

更新日期：2020-11-22 00:00:00

abstract：:The recently identified recA gene of the naturally transformable bacterium Streptococcus pneumoniae has been further characterized by constructing a recA null mutation and by investigating its regulation. The recA mutation has been shown to confer both DNA repair (as judged from sensitivity to u.v. and methyl methane ...

journal_title：Molecular microbiology

authors： Martin B,García P,Castanié MP,Claverys JP

更新日期：1995-01-01 00:00:00

abstract：:Integration host factor (IHF) binds to two sites near the origin of transfer of the conjugative antibiotic resistance plasmid, R100. DNase I footprinting shows that one site is immediately adjacent to oriT and the gene X promoter, and another is adjacent to the traM promoter. A third site, known only from retardation ...

journal_title：Molecular microbiology

authors： Dempsey WB,Fee BE

更新日期：1990-06-01 00:00:00

abstract：:Copy-number mutants of Staphylococcus aureus macrolide-lincosamide-streptogramin B (MLS) resistance plasmid pT48 were isolated by their resistance to the non-inducing macrolide, tylosin. One mutant plasmid, pcopD3, showed a three- to five-fold cis-dominant increase in copy number, and nucleotide sequence analysis reve...

journal_title：Molecular microbiology

authors： Catchpole I,Dyke KG

更新日期：1991-04-01 00:00:00

abstract：:The Rhizobium leguminosarum biovar phaseoli symbiotic plasmid pRP2JI carries a gene, melA, specifying the enzyme tyrosinase, which is responsible for the production of the pigment melanin in these bacteria. Transcription of melA is activated by the nifA gene of Rhizobium and, when the cloned melA gene is transferred t...

journal_title：Molecular microbiology

authors： Hawkins FK,Johnston AW

更新日期：1988-05-01 00:00:00

abstract：:Previous studies have shown that in Saccharomyces cerevisiae the mitochondrial and cytoplasmic forms of alanyl-tRNA synthetase are encoded by a single nuclear gene, ALA1, through alternative use of in-frame successive ACG triplets and a downstream AUG triplet. Here we show that despite the obvious participation of the...

journal_title：Molecular microbiology

authors： Huang HY,Tang HL,Chao HY,Yeh LS,Wang CC

更新日期：2006-04-01 00:00:00

abstract：:In order to reveal functional properties of recombination involving short ssDNAs in hyperthermophilic archaea, we evaluated oligonucleotide-mediated transformation (OMT) in Sulfolobus acidocaldarius and Escherichia coli as a function of the molecular properties of the ssDNA substrates. Unmodified ssDNAs as short as 20...

journal_title：Molecular microbiology

authors： Grogan DW,Stengel KR

更新日期：2008-09-01 00:00:00