Heterozygosity and functional allelic variation in the Candida albicans efflux pump genes CDR1 and CDR2.

abstract：:F plasmid traY protein binding to wild-type or deleted regions containing the TraY-binding site, sbyA, was studied in vitro. The principal DNA-protein complex was formed with DNA segments including the sbyA site defined by footprinting and (with lesser affinity) with truncated segments that retained the leftward two-t...

journal_title：Molecular microbiology

authors： Luo Y,Gao Q,Deonier RC

更新日期：1994-02-01 00:00:00

abstract：:Transfer RNAs (tRNAs) are the macromolecules that transfer activated amino acids from aminoacyl-tRNA synthetases to the ribosome, where they are used for the mRNA guided synthesis of proteins. Transfer RNAs are ancient molecules, perhaps even predating the existence of the translation machinery. Albeit old, these mole...

journal_title：Molecular microbiology

authors： Katz A,Elgamal S,Rajkovic A,Ibba M

更新日期：2016-08-01 00:00:00

abstract：:SsrA is a tmRNA involved in tagging polypeptides on stalled ribosomes. The resulting fusion proteins are then degraded. We purified endogenous SsrA-tagged proteins by means of a genetically engineered SsrA and identified some of them. Analysis of the proteins suggested that they are tagged at their C-terminal extremit...

journal_title：Molecular microbiology

authors： Collier J,Binet E,Bouloc P

更新日期：2002-08-01 00:00:00

abstract：:The dominant, polA1-independent replicon of pGSH500, rep beta (1.8 kb), consists of a cis-acting oriV region of 245 bp; a repB gene that is essential for autonomous replication and 18, 30 to 36 bp iterons which constitute the inc/cop region. The molecular organization of rep beta resembles that of mini-pCU1 (IncN). Fu...

journal_title：Molecular microbiology

authors： da Silva-Tatley FM,Steyn LM

更新日期：1993-03-01 00:00:00

abstract：:RNase protection experiments show that the sizes of the two R100 finP molecules are 74 and 135 nucleotides. In an RNase III mutant, finP transcripts form stable double-stranded hybrids of 108 bp and 68 bp with traJ transcripts. RNase protection experiments also show that most R100-1 transcripts originating in traM cro...

journal_title：Molecular microbiology

authors： Dempsey WB

更新日期：1994-07-01 00:00:00

abstract：:The rfb (O antigen) gene cluster of group C2 Salmonella differs from that of group B in a central region of 12.4 kb: we report the sequencing of this region of strain M67 (group C2) and a subsequent comparison with the central region of strain LT2 (group B). We find a block of seven open reading frames unique to group...

journal_title：Molecular microbiology

authors： Brown PK,Romana LK,Reeves PR

更新日期：1992-05-01 00:00:00

abstract：:Glutaredoxins and thioredoxins are small heat-stable oxidoreductases that have been conserved throughout evolution. The yeast Saccharomyces cerevisiae contains two gene pairs encoding cytoplasmic glutaredoxins (GRX1, GRX2) and thioredoxins (TRX1, TRX2). We report here that the quadruple trx1 trx2 grx1 grx2 mutant is i...

journal_title：Molecular microbiology

authors： Draculic T,Dawes IW,Grant CM

更新日期：2000-06-01 00:00:00

abstract：:The guinea pig intestinal symbiont Metabacterium polyspora is an uncultured, endospore-forming member of the Firmicutes. Unlike most endospore-forming bacteria, sporulation is an obligate part of the M. polyspora life cycle when it is associated with a guinea pig. Binary fission is limited to a brief period in its lif...

journal_title：Molecular microbiology

authors： Ward RJ,Angert ER

更新日期：2008-03-01 00:00:00

abstract：:The Salmonella enterica serotype Typhimurium (S. typhimurium) genome contains a large repertoire of putative fimbrial operons that remain poorly characterized because they are not expressed in vitro. In this study, insertions that induced expression of the putative stdABCD fimbrial operon were identified from a random...

journal_title：Molecular microbiology

authors： Chessa D,Winter MG,Nuccio SP,Tükel C,Bäumler AJ

更新日期：2008-05-01 00:00:00

abstract：:The K1 killer toxin of Saccharomyces cerevisiae consists of 103- and 83-residue alpha and beta components whose derivation, from a 316-residue precursor preprotoxin, requires processing at the alpha N-terminus (after ProArg-44), the alpha C-terminus (after ArgArg-149) and at the beta N-terminus (after LysArg-233). The...

journal_title：Molecular microbiology

authors： Zhu YS,Zhang XY,Cartwright CP,Tipper DJ

更新日期：1992-02-01 00:00:00

abstract：:Type IV secretion systems are virulence determinants in many bacteria and share extensive homology with many conjugal transfer systems. Although type IV systems and their homologues have been studied widely, the mechanism by which substrates are secreted remains unclear. In Agrobacterium, we show that type IV secretio...

journal_title：Molecular microbiology

authors： Pantoja M,Chen L,Chen Y,Nester EW

更新日期：2002-09-01 00:00:00

abstract：:The recently identified recA gene of the naturally transformable bacterium Streptococcus pneumoniae has been further characterized by constructing a recA null mutation and by investigating its regulation. The recA mutation has been shown to confer both DNA repair (as judged from sensitivity to u.v. and methyl methane ...

journal_title：Molecular microbiology

authors： Martin B,García P,Castanié MP,Claverys JP

更新日期：1995-01-01 00:00:00

abstract：:Crop destruction by the hemibiotrophic rice pathogen Magnaporthe oryzae requires plant defence suppression to facilitate extensive biotrophic growth in host cells before the onset of necrosis. How this is achieved at the genetic level is not well understood. Here, we report that a M. oryzae sirtuin, MoSir2, plays an e...

journal_title：Molecular microbiology

authors： Fernandez J,Marroquin-Guzman M,Nandakumar R,Shijo S,Cornwell KM,Li G,Wilson RA

更新日期：2014-10-01 00:00:00

abstract：:The fimbrial and afimbrial adhesins of the Dr family mediate the adherence of uropathogenic and diarrhoea-associated Escherichia coli to decay-accelerating factor (DAF) present on erythrocytes and other cell types. The Dr haemagglutinin binds type IV collagen and, unlike other members of the Dr family, mediates an adh...

journal_title：Molecular microbiology

authors： Carnoy C,Moseley SL

更新日期：1997-01-01 00:00:00

abstract：:Motile bacteria sense their physical and chemical environment through highly cooperative, ordered arrays of chemoreceptors. These signalling complexes phosphorylate a response regulator which in turn governs flagellar motor reversals, driving cells towards favourable environments. The structural changes that translate...

journal_title：Molecular microbiology

authors： Briegel A,Ames P,Gumbart JC,Oikonomou CM,Parkinson JS,Jensen GJ

更新日期：2013-09-01 00:00:00

abstract：:A series of lipoproteins was detected in the membrane fraction of Yersinia enterocolitica W227, a typical strain from serotype O:9. At least two of them, YlpA and YlpB, are encoded by the pYV plasmid. The sequence of ylpA reveals the presence of a typical lipoprotein signal peptide. The mature YlpA protein would be 22...

journal_title：Molecular microbiology

authors： China B,Michiels T,Cornelis GR

更新日期：1990-09-01 00:00:00

abstract：:The majority of bacterial genomes encode a high number of two-component systems controlling gene expression in response to a variety of different stimuli. The Gram-positive soil bacterium Corynebacterium glutamicum contains two homologous two-component systems (TCS) involved in the haem-dependent regulation of gene ex...

journal_title：Molecular microbiology

authors： Hentschel E,Mack C,Gätgens C,Bott M,Brocker M,Frunzke J

更新日期：2014-06-01 00:00:00

abstract：:In trying to identify genetic loci involved in the regulation of cap5 genes in Staphylococcus aureus, we isolated a transposon mutant that exhibited a growth defect, enhanced autolysis and increased sensitivity to Triton X-100 and penicillin, attributable in part to increased murein hydrolase activity. Analysis of the...

journal_title：Molecular microbiology

authors： Ingavale SS,Van Wamel W,Cheung AL

更新日期：2003-06-01 00:00:00

abstract：:In prokaryotes, the principal signal transduction systems operating at the level of protein phosphorylation are the two-component systems. A number of hybrid histidine protein kinases in these systems contain several receiver domains, however, the function of these receiver domains is unknown. The RodK kinase in Myxoc...

journal_title：Molecular microbiology

authors： Rasmussen AA,Wegener-Feldbrügge S,Porter SL,Armitage JP,Søgaard-Andersen L

更新日期：2006-04-01 00:00:00

abstract：:In mammalian cells RACK1 serves as a scaffold protein that has a role in integrating inputs from different signalling pathways and affects translation through association with ribosomes. Ustilago maydis contains a seven-WD40 repeat motif protein designated Rak1, which shows 68% identity to RACK1 and 51% identity to As...

journal_title：Molecular microbiology

authors： Wang L,Berndt P,Xia X,Kahnt J,Kahmann R

更新日期：2011-09-01 00:00:00

abstract：:Members of the bacterial genus Brucella are facultative intracellular pathogens that reside predominantly within membrane-bound compartments within two host cell types, macrophages and placental trophoblasts. Within macrophages, the brucellae route themselves to an intracellular compartment that is favourable for surv...

journal_title：Molecular microbiology

authors： Roop RM 2nd,Bellaire BH,Valderas MW,Cardelli JA

更新日期：2004-05-01 00:00:00

abstract：:We have demonstrated hypervariability of native short-motif repeats (microsatellites) in Escherichia coli. Twenty-five of the longest microsatellites in the E. coli genome were identified. These were analysed for length variability among 22 wild-type (non-mutator) isolates from the E. coli collection of reference (ECO...

journal_title：Molecular microbiology

authors： Metzgar D,Thomas E,Davis C,Field D,Wills C

更新日期：2001-01-01 00:00:00

abstract：:Filamentous hemagglutinin (FHA) is a critically important virulence factor produced by Bordetella species that cause respiratory infections in humans and other animals. It is also a prototypical member of the widespread two partner secretion (TPS) pathway family of proteins. First synthesized as a ~370 kDa protein cal...

journal_title：Molecular microbiology

authors： Nash ZM,Cotter PA

更新日期：2019-09-01 00:00:00

abstract：:The importance of methylthioadenosine/S-adenosylhomocysteine (MTA/SAH) nucleosidase in bacteria has started to be appreciated only in the past decade. A comprehensive analysis of its various roles here demonstrates that it is an integral component of the activated methyl cycle, which recycles adenine and methionine th...

journal_title：Molecular microbiology

authors： Parveen N,Cornell KA

更新日期：2011-01-01 00:00:00

abstract：:Maturation of c-type cytochromes in Escherichia coli is a complex process requiring eight membrane proteins encoded by the ccmABCDEFGH operon. CcmE is a mediator of haem delivery. It binds haem transiently at a conserved histidine residue and releases it for directed transfer to apocytochrome c. CcmC, an integral memb...

journal_title：Molecular microbiology

authors： Schulz H,Pellicioli EC,Thöny-Meyer L

更新日期：2000-09-01 00:00:00

abstract：:The flexible pilus of Aeromonas hydrophila is a morphologically and biochemically unique organelle which binds eukaryotic cell surfaces and whose expression is induced by specific physiochemical conditions. fxp, the structural gene coding for the flexible pilus subunit, was localized on a 7.6kb plasmid of A. hydrophil...

journal_title：Molecular microbiology

authors： Ho AS,Sohel I,Schoolnik GK

更新日期：1992-09-01 00:00:00

abstract：:Fungi appear to be unique in their requirement for a third soluble translation elongation factor. This factor, designated elongation factor 3 (EF-3), was first described in the yeast Saccharomyces cerevisiae and has subsequently been identified in a wide range of fungal species including Candida albicans and Schizosac...

journal_title：Molecular microbiology

authors： Belfield GP,Tuite MF

更新日期：1993-08-01 00:00:00

abstract：:A challenge common to all bacterial pathogens is to acquire nutrients from hostile host environments. Iron is an important cofactor required for essential cellular processes such as DNA repair, energy production and redox balance. Within a mammalian host, most iron is sequestered within heme, which in turn is predomin...

journal_title：Molecular microbiology

authors： Clark J,Terwilliger A,Nguyen C,Green S,Nobles C,Maresso A

更新日期：2019-08-01 00:00:00

abstract：:Individual segments of the polycistronic puf mRNA of Rhodobacter capsulatus exhibit extremely different half-lives contributing to the stoichiometry of light-harvesting and reaction centre complexes of this facultative phototrophic bacterium. While earlier investigations shed light on the processes leading to the degr...

journal_title：Molecular microbiology

authors： Heck C,Balzer A,Fuhrmann O,Klug G

更新日期：2000-01-01 00:00:00

abstract：:In addition to being the major citric acid cycle aconitase in Escherichia coli the aconitase B protein (AcnB) is also a post-transcriptional regulator of gene expression. The AcnB proteins represent a distinct branch of the aconitase superfamily that possess a HEAT-like domain (domain 5). The HEAT domains of other pro...

journal_title：Molecular microbiology

authors： Tang Y,Guest JR,Artymiuk PJ,Green J

更新日期：2005-06-01 00:00:00