Primary and secondary metabolism, and post-translational protein modifications, as portrayed by proteomic analysis of Streptomyces coelicolor.

abstract：:Myxococcus xanthus is a Gram-negative, soil-dwelling bacterium with a complex life cycle which includes fruiting body formation and sporulation in response to starvation. This developmental process is slow, requiring a minimum of 24-48 h, and requires cells to be at high cell density on a solid surface. It is known th...

journal_title：Molecular microbiology

authors： O'Connor KA,Zusman DR

更新日期：1997-05-01 00:00:00

abstract：:PII-like signalling molecules are trimeric proteins composed of 12-13 kDa polypeptides encoded by the glnB gene family. Heterologous expression of a cyanobacterial glnB gene in Escherichia coli leads to an inactivation of E. coli's own PII signalling system. In the present work, we show that this effect is caused by t...

journal_title：Molecular microbiology

authors： Forchhammer K,Hedler A,Strobel H,Weiss V

更新日期：1999-07-01 00:00:00

abstract：:By genetic analysis of a thermosensitive autolytic mutant whose phenotype was complemented by osmotic stabilization with sorbitol, we identified gene LYT2 of Saccharomyces cerevisiae, which is probably involved in cell wall formation. A yeast gene complementing lyt2 strains was cloned and shown to carry an open readin...

journal_title：Molecular microbiology

authors： Torres L,Martín H,García-Saez MI,Arroyo J,Molina M,Sánchez M,Nombela C

更新日期：1991-11-01 00:00:00

abstract：:A 4.0 kb EcoRI fragment of Bacillus subtilis conferring thiostrepton resistance was cloned and characterized. By nucleotide sequencing of the relevant region, six open reading frames were established, which corresponded to a part of spo0H, a ribosomal protein gene (rpmG), an unidentified open reading frame (orfE), a t...

journal_title：Molecular microbiology

authors： Jeong SM,Yoshikawa H,Takahashi H

更新日期：1993-10-01 00:00:00

abstract：:We have devised a simple method for the reconstitution of bacterial membrane proteins directly from small (1-20 ml) volumes of cell culture, thus eliminating the preparation of membrane vesicles. Cells are subjected to simultaneous lysozyme digestion and osmotic lysis, and after brief centrifugation ghosts are solubil...

journal_title：Molecular microbiology

authors： Varadhachary A,Maloney PC

更新日期：1990-08-01 00:00:00

abstract：:Clp ATPases, which include the ubiquitous HSP100 family, are classified according to their structural features and sequence similarities. During the course of the Bacillus subtilis genome sequencing project, we identified a gene encoding a new member of the HSP100 family. We designated this protein ClpE, as it is the ...

journal_title：Molecular microbiology

authors： Derré I,Rapoport G,Devine K,Rose M,Msadek T

更新日期：1999-05-01 00:00:00

abstract：:The F factor traY gene product (TraYp) is a site-specific DNA-binding protein involved in initiation of DNA transfer during bacterial conjugation. The sequence of TraYp exhibits a unique direct-repeat structure predicted to have a ribbon-helix-helix DNA-binding motif in each repeat unit. The stoichiometry of TraYp bin...

journal_title：Molecular microbiology

authors： Nelson WC,Matson SW

更新日期：1996-06-01 00:00:00

abstract：:A fragment of mitochondrial DNA containing the Kluyveromyces lactis gene for valine-tRNA (tRNAVAL) was isolated as a multicopy suppressor of a respiratory-deficient mutant of this yeast. The mutant produced a truncated Cox14p because of a +1 frameshift mutation in COX14, a nuclear gene encoding a protein imported into...

journal_title：Molecular microbiology

authors： Fiori A,Francisci S,Falcone C

更新日期：2002-10-01 00:00:00

abstract：:TonB protein functions as an energy transducer, coupling cytoplasmic membrane electrochemical potential to the active transport of vitamin B12 and Fe(III)-siderophore complexes across the outer membrane of Escherichia coli and other Gram-negative bacteria. Accumulated evidence indicates that TonB is anchored in the cy...

journal_title：Molecular microbiology

authors： Larsen RA,Wood GE,Postle K

更新日期：1993-12-01 00:00:00

abstract：:Phycobilisomes are the major constituents of the light-harvesting apparatus in both cyanobacteria and red algae and consist of a central core with radiating rods. From a genomic library of the cyanobacterium Calothrix 7601, a DNA fragment encoding allophycocyanin B, one of the two terminal energy acceptors of the core...

journal_title：Molecular microbiology

authors： Houmard J,Capuano V,Coursin T,Tandean de Marsac N

更新日期：1988-01-01 00:00:00

abstract：:In eukaryotes, frataxin deficiency (FXN) causes severe phenotypes including loss of iron-sulfur (Fe-S) cluster protein activity, accumulation of mitochondrial iron and leads to the neurodegenerative disease Friedreich's ataxia. In contrast, in prokaryotes, deficiency in the FXN homolog, CyaY, was reported not to cause...

journal_title：Molecular microbiology

authors： Roche B,Huguenot A,Barras F,Py B

更新日期：2015-02-01 00:00:00

abstract：:Ustilago maydis, the causal agent of corn smut disease, displays dimorphic growth in which it alternates between a budding haploid saprophyte and a filamentous dikaryotic pathogen. We are interested in identifying the genetic determinants of filamentous growth and pathogenicity in U. maydis. To do this, we have taken ...

journal_title：Molecular microbiology

authors： Mayorga ME,Gold SE

更新日期：1999-11-01 00:00:00

abstract：:Regions close to the replication terminus of the Escherichia coli chromosome are strongly refractory to genomic inversions. Since these regions also harbour polar replication terminator-like sequences or pause sites, we have investigated the possibility that slowing of replication as a result of pausing at inverted pa...

journal_title：Molecular microbiology

authors： François V,Louarn J,Patte J,Rebollo JE,Louarn JM

更新日期：1990-04-01 00:00:00

abstract：:In the budding yeast Saccharomyces cerevisiae, the preservation of the mating competent haploid (a or alpha) and the mating incompetent diploid (a/alpha) is necessary to prevent aneuploidy. Once haploid cells respond to pheromone, the mating-specific signal transduction pathway is activated, and the MAP kinase Fus3 ph...

journal_title：Molecular microbiology

authors： Gelli A

更新日期：2002-11-01 00:00:00

abstract：:We have previously shown that in the human pathogen Neisseria meningitidis group B (MenB) more than 200 genes are regulated in response to growth with iron. Among the Fur-dependent, upregulated genes identified by microarray analysis was a putative operon constituted by three genes, annotated as NMB1436, NMB1437 and N...

journal_title：Molecular microbiology

authors： Grifantini R,Frigimelica E,Delany I,Bartolini E,Giovinazzi S,Balloni S,Agarwal S,Galli G,Genco C,Grandi G

更新日期：2004-11-01 00:00:00

abstract：:A series of internal deletions in the lktA gene of Pasteurella haemolytica has been constructed. All of the deletions eliminated the lytic activity of the leukotoxin towards the bovine lymphoma cell line, BL-3. Deletions removing segments of the amino-proximal hydrophobic region, which is thought to constitute an esse...

journal_title：Molecular microbiology

authors： Cruz WT,Young R,Chang YF,Struck DK

更新日期：1990-11-01 00:00:00

abstract：:Type III secretion systems are found in many Gram-negative bacteria. They are activated by contact with eukaryotic cells and inject virulence proteins inside them. Host cell detection requires a protein complex located at the tip of the device's external injection needle. The Shigella tip complex (TC) is composed of I...

journal_title：Molecular microbiology

authors： Cheung M,Shen DK,Makino F,Kato T,Roehrich AD,Martinez-Argudo I,Walker ML,Murillo I,Liu X,Pain M,Brown J,Frazer G,Mantell J,Mina P,Todd T,Sessions RB,Namba K,Blocker AJ

更新日期：2015-01-01 00:00:00

abstract：:The enteric gamma-proteobacterium Photorhabdus luminescens kills a wide range of insects, whilst also maintaining a mutualistic relationship with soil nematodes from the family Heterorhabditis. Pathogenicity is associated with bacterial exponential growth, whilst mutualism is associated with post-exponential (stationa...

journal_title：Molecular microbiology

authors： Bager R,Roghanian M,Gerdes K,Clarke DJ

更新日期：2016-05-01 00:00:00

abstract：:Malaria transmission requires that the parasites differentiate into gametocytes prior to ingestion by a mosquito during a blood meal. Once in the mosquito midgut the gametocytes emerge from red blood cells (RBCs), fertilize, develop into ookinetes and finally infectious sporozoites. Gamete surface antigen, Pfs230, is ...

journal_title：Molecular microbiology

authors： Eksi S,Czesny B,van Gemert GJ,Sauerwein RW,Eling W,Williamson KC

更新日期：2006-08-01 00:00:00

abstract：:Vibrio cholerae, the causative agent of the acute diarrheal disease cholera, is able to thrive in diverse habitats such as natural water bodies and inside human hosts. To ensure their survival, these bacteria rely on chemosensory pathways to sense and respond to changing environmental conditions. These pathways consti...

journal_title：Molecular microbiology

authors： Ortega DR,Kjaer A,Briegel A

更新日期：2020-09-01 00:00:00

abstract：:The K1 killer toxin of Saccharomyces cerevisiae consists of 103- and 83-residue alpha and beta components whose derivation, from a 316-residue precursor preprotoxin, requires processing at the alpha N-terminus (after ProArg-44), the alpha C-terminus (after ArgArg-149) and at the beta N-terminus (after LysArg-233). The...

journal_title：Molecular microbiology

authors： Zhu YS,Zhang XY,Cartwright CP,Tipper DJ

更新日期：1992-02-01 00:00:00

abstract：:The differences between archaeal and bacterial flagella are becoming more apparent as research on the archaeal structure progresses. One crucial difference is the presence of a leader peptide on archaeal preflagellins, which is removed from the flagellin prior to its incorporation into the flagellar filament. The enzy...

journal_title：Molecular microbiology

authors： Bardy SL,Jarrell KF

更新日期：2003-11-01 00:00:00

abstract：:This review assembles data from three bodies of literature (bacterial genetics, plastid biogenesis and parasitology) that seldom have much direct cross-talk. After overcoming terminological complications to sort out microbial nifS from sufS genes, we connect a bacterial operon, recently found to be involved in iron me...

journal_title：Molecular microbiology

authors： Ellis KE,Clough B,Saldanha JW,Wilson RJ

更新日期：2001-09-01 00:00:00

abstract：:The Saccharomyces cerevisiae RAP1 protein (Rap1p) is a key multifunctional transcription factor. Using gel retardation analysis, four binding sites for Rap1p have been identified within the promoter of the RAP1 gene. These sites are located downstream of a binding site for the transcription factor Reb1p. The Reb1p sit...

journal_title：Molecular microbiology

authors： Graham IR,Chambers A

更新日期：1994-06-01 00:00:00

abstract：:Proteins with LytM (Peptidase_M23) domains are broadly distributed in bacteria and have been implicated in a variety of important processes, including cell division and cell-shape determination. Most LytM-like proteins that have been structurally and/or biochemically characterized are metallo-endopeptidases that cleav...

journal_title：Molecular microbiology

authors： Peters NT,Morlot C,Yang DC,Uehara T,Vernet T,Bernhardt TG

更新日期：2013-08-01 00:00:00

abstract：:Enhancement of enzyme thermostability by protein engineering gives us information about the thermostabilization mechanism as well as advantages for industrial use of enzymes. In this study, we enhanced the thermostability of endoglucanase EngB, one component of the cellulase complex (cellulosome) from Clostridium cell...

journal_title：Molecular microbiology

authors： Murashima K,Kosugi A,Doi RH

更新日期：2002-08-01 00:00:00

abstract：:The genome of the Bacillus subtilis 168-type strain contains 10 ribosomal RNA (rRNA) operons. In the intergenic spacer region (ISR) between the 16S and 23S rRNA genes, five rRNA operons, rrnI-H-G and rrnJ-W, lack a trinucleotide signature region. Precise determination of molecular weight (MW), using electrospray mass ...

journal_title：Molecular microbiology

authors： Shaver YJ,Nagpal ML,Fox KF,Rudner R,Fox A

更新日期：2001-10-01 00:00:00

abstract：:In enteric bacteria, proteins of the Hha/YmoA family play a role in the regulation of gene expression in response to environmental factors. Interaction of both Hha and YmoA with H-NS has been reported, and an Hha/H-NS complex has been shown to modulate expression in Escherichia coli of the haemolysin operon of plasmid...

journal_title：Molecular microbiology

authors： Paytubi S,Madrid C,Forns N,Nieto JM,Balsalobre C,Uhlin BE,Juárez A

更新日期：2004-10-01 00:00:00

abstract：:Transposon insertion mutagenesis and transformation were used to locate genes responsible for excision in the temperature phage HP1 of Haemophilus influenzae. A 6.5 kb segment of DNA near the left end of the phage genome was sequenced, and 11 new open reading frames were identified. Two face-to-face overlapping promot...

journal_title：Molecular microbiology

authors： Esposito D,Scocca JJ

更新日期：1994-08-01 00:00:00

abstract：:Cell division in prokaryotes initiates with assembly of the Z-ring at midcell, which, in Escherichia coli, is tethered to the inner leaflet of the cytoplasmic membrane through a direct interaction with FtsA, a widely conserved actin homolog. The Z-ring is comprised of polymers of tubulin-like FtsZ and has been suggest...

journal_title：Molecular microbiology

authors： Conti J,Viola MG,Camberg JL

更新日期：2018-02-01 00:00:00