Abstract:
:A gene bank of Azorhizobium caulinodans DNA constructed in the bacteriophage lambda GEM11 was screened with Rhizobium meliloti fixL and fixJ genes as probes. One positive recombinant phage, ORS lambda L, was isolated. The nucleotide sequence of a 3.7 kb fragment was established. Two open reading frames of 1512bp and 613bp were identified as fixL and fixJ. Kanamycin cartridges were inserted into the cloned fixL and fixJ genes and recombined into the host genome. The resulting mutants were Nif- Fix-, suggesting that the two genes were required for symbiotic nitrogen fixation and for nitrogen fixation in the free-living state. Using pnifH-lacZ and pnifA-lacZ fusions, it was shown that the FixLJ products controlled the expression of nifH and nifA in bacteria grown in the free-living state.
journal_name
Mol Microbioljournal_title
Molecular microbiologyauthors
Kaminski PA,Elmerich Cdoi
10.1111/j.1365-2958.1991.tb00738.xsubject
Has Abstractpub_date
1991-03-01 00:00:00pages
665-73issue
3eissn
0950-382Xissn
1365-2958journal_volume
5pub_type
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