Abstract：
:Fibronectin-binding proteins (FnBPs) are thought to be important for the attachment of Staphylococcus aureus during infection. The regulation of the genes fnbA and fnbB by the global regulatory loci sar and agr was examined using site-specific regulatory mutants of S. aureus strain Newman. The results from binding assays using both aqueous and solid-phase fibronectin as well as ligand blotting with biotinylated fibronectin showed that the expression of FnBPA is enhanced in the agr mutant but inhibited in the sar mutant and the sar-agr double mutant. The same regulatory pattern was observed in Northern blot analysis using fnbA-specific probes. The introduction of sar on a multicopy plasmid increased the already enhanced fnbA transcription of the agr mutant. FnBPB was not detectable by ligand blotting and the fnbB promoter activity in promoter fusion assays was not affected by either sar or agr. The sequence encompassing ORF3 located upstream of sarA was found to be essential for the activation of fnbA transcription. We hypothesize that this sequence may modulate SarA expression and/or activity on the post-transcriptional level. Gel shift assays demonstrated that SarA binds to the fnbA promoter fragments, probably as a dimer. DNase I footprinting assays with SarA revealed a protected area of 102 bp upstream of fnbA.
journal_name
Mol Microbioljournal_title
Molecular microbiologyauthors
Wolz C,Pöhlmann-Dietze P,Steinhuber A,Chien YT,Manna A,van Wamel W,Cheung Adoi
10.1046/j.1365-2958.2000.01853.xsubject
Has Abstractpub_date
2000-04-01 00:00:00pages
230-43issue
1eissn
0950-382Xissn
1365-2958pii
mmi1853journal_volume
36pub_type
杂志文章abstract：:Streptococcus pyogenes interacts with host fibronectin via distinct surface components. One of these components is the Sfbl protein (streptococcal fibronectin-binding protein, now specified as class I), an adhesin that represents a protein family with characteristic features. Here we present the complete structure of ...
journal_title：Molecular microbiology
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pub_type： 杂志文章
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更新日期：1992-05-01 00:00:00
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更新日期：1989-11-01 00:00:00
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journal_title：Molecular microbiology
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更新日期：2020-01-01 00:00:00
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