Abstract：
:BldD generally functions as a repressor controlling morphological development of Streptomyces. In this work, evidences that BldD also activates antibiotic production are provided. In Streptomyces roseosporus (which produces daptomycin widely used for treatment of human infections), deletion of bldD notably reduced daptomycin production, but enhanced sporulation. BldD stimulated daptomycin production by directly activating transcription of dpt structural genes and dptR3 (which encodes an indirect activator of daptomycin production), and repressed its own gene. BldD-binding sites on promoter regions of dptE, dptR3, and bldD were all found to contain BldD box-like sequences, facilitating prediction of new BldD targets. Two Streptomyces global regulatory genes, adpA and afsR, were confirmed to be directly activated by BldD. The protein AfsR was shown to act as an activator of daptomycin production, but a repressor of development. BldD directly represses nine key developmental genes. In Streptomyces avermitilis (which produces effective anthelmintic agents avermectins), BldD homolog (BldDsav) directly activates avermectin production through ave structural genes and cluster-situated activator gene aveR. This is the first report that BldD activates antibiotic biosynthesis both directly and via a cascade mechanism. BldD homologs are widely distributed among Streptomyces, our findings suggest that BldD may activate antibiotic production in other Streptomyces species.
journal_name
Mol Microbioljournal_title
Molecular microbiologyauthors
Yan H,Lu X,Sun D,Zhuang S,Chen Q,Chen Z,Li J,Wen Ydoi
10.1111/mmi.14405subject
Has Abstractpub_date
2020-01-01 00:00:00pages
123-142issue
1eissn
0950-382Xissn
1365-2958journal_volume
113pub_type
杂志文章abstract：:We have used a targeted gene deletion event to remove the coding region for the bgl1 gene encoding an extracellular beta-glucosidase from the genome of the cellulolytic fungus Trichoderma reesei. The bgl1 null mutants were used to investigate the role of beta-glucosidase in the hydrolysis of cellulose and induction of...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.1992.tb01777.x
更新日期：1992-11-01 00:00:00
abstract：:N-linked protein glycosylation occurs in all three branches of life, eukaryotes, bacteria and archaea. The simplest system is that of the bacterium, Campylobacter jejuni, in which a heptasaccharide glycan is added to multiple proteins from a single lipid carrier molecule. In the eukaryotic system a conserved tetradeca...
journal_title：Molecular microbiology
pub_type： 评论,杂志文章
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pub_type： 杂志文章
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pub_type： 杂志文章
doi：10.1111/j.1365-2958.1991.tb01993.x
更新日期：1991-11-01 00:00:00
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journal_title：Molecular microbiology
pub_type： 杂志文章
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更新日期：1991-10-01 00:00:00
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pub_type： 杂志文章
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journal_title：Molecular microbiology
pub_type： 杂志文章,评审
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pub_type： 杂志文章
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