The ObgE/CgtA GTPase influences the stringent response to amino acid starvation in Escherichia coli.

Abstract:

:The stringent response is important for bacterial survival under stressful conditions, such as amino acid starvation, and is characterized by the accumulation of ppGpp and pppGpp. ObgE (CgtA, YhbZ) is an essential conserved GTPase in Escherichia coli and several observations have implicated the protein in the control of the stringent response. However, consequences of the protein on specific responses to amino acid starvation have not been noted. We show that ObgE binds to ppGpp with biologically relevant affinity in vitro, implicating ppGpp as an in vivo ligand of ObgE. ObgE mutants increase the ratio of pppGpp to ppGpp within the cell during the stringent response. These changes are correlated with a delayed inhibition of DNA replication by the stringent response, delayed resumption of DNA replication after release, as well as a decreased survival after amino acid deprivation. With these data, we place ObgE as an active effector of the response to amino acid starvation in vivo. Our data correlate the pppGpp/ppGpp ratio with DNA replication control under bacterial starvation conditions, suggesting a possible role for the relative balance of these two nucleotides.

journal_name

Mol Microbiol

journal_title

Molecular microbiology

authors

Persky NS,Ferullo DJ,Cooper DL,Moore HR,Lovett ST

doi

10.1111/j.1365-2958.2009.06767.x

subject

Has Abstract

pub_date

2009-07-01 00:00:00

pages

253-66

issue

2

eissn

0950-382X

issn

1365-2958

pii

MMI6767

journal_volume

73

pub_type

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