Abstract:
:By genetic analysis of a thermosensitive autolytic mutant whose phenotype was complemented by osmotic stabilization with sorbitol, we identified gene LYT2 of Saccharomyces cerevisiae, which is probably involved in cell wall formation. A yeast gene complementing lyt2 strains was cloned and shown to carry an open reading frame coding for a 484-amino-acid protein exhibiting all the characteristic domains of serine/threonine protein kinases and highly homologous to other yeast protein kinases involved in control of the mitotic cycle. Mutants disrupted in the cloned gene also displayed an autolytic phenotype complemented by osmotic stabilization with sorbitol. However, genetic comparison of lyt2 mutants and disruptants of the protein kinase gene revealed that the cloned gene is not the structural gene LYT2 but a suppressor of the lytic phenotype, named gene SLT2, that was mapped to chromosome V. The product of gene SLT2 is the first protein kinase to be described in relation to the yeast cell-wall functions.
journal_name
Mol Microbioljournal_title
Molecular microbiologyauthors
Torres L,Martín H,García-Saez MI,Arroyo J,Molina M,Sánchez M,Nombela Cdoi
10.1111/j.1365-2958.1991.tb01993.xsubject
Has Abstractpub_date
1991-11-01 00:00:00pages
2845-54issue
11eissn
0950-382Xissn
1365-2958journal_volume
5pub_type
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