Tn10/IS10 transposition is downregulated at the level of transposase expression by the RNA-binding protein Hfq.

abstract：:The Escherichia coli Sm-like host factor I (Hfq) is thought to play direct and indirect roles in post-transcriptional regulation by targeting small regulatory RNAs and mRNAs. In this study, we have used proteomics to identify new mRNA targets of Hfq. We have identified 11 candidate proteins, synthesis of which was dif...

journal_title：Molecular microbiology

authors： Vecerek B,Moll I,Afonyushkin T,Kaberdin V,Bläsi U

更新日期：2003-11-01 00:00:00

abstract：:Staphylococcus aureus is a leading cause of infective endocarditis (IE). Platelet activation promoted by S. aureus resulting in aggregation and thrombus formation is an important step in the pathogenesis of IE. Here, we report that the fibrinogen/fibronectin-binding proteins FnBPA and FnBPB are major platelet-activati...

journal_title：Molecular microbiology

authors： Fitzgerald JR,Loughman A,Keane F,Brennan M,Knobel M,Higgins J,Visai L,Speziale P,Cox D,Foster TJ

更新日期：2006-01-01 00:00:00

abstract：:We found that mutations that increased the transcription of the mgtCBR (Mg2+ transport-related) operon conferred increased thermotolerance on this organism. The 5' leader of the mgtCBR mRNA contains two short open reading frames (ORFs), mgtM and mgtP, whose translation regulates the expression of the mgtCBR operon by ...

journal_title：Molecular microbiology

authors： Gall AR,Hegarty AE,Datsenko KA,Westerman RP,SanMiguel P,Csonka LN

更新日期：2018-08-01 00:00:00

abstract：:Nitrogen metabolism in Aspergillus nidulans is regulated by AREA, a member of the GATA family of transcription factors. One mechanism that modulates AREA activity involves the rapid degradation of the areA transcript when sufficient NH4+ or Gln are available. This signalling mechanism has been shown to require a regio...

journal_title：Molecular microbiology

authors： Morozov IY,Martinez MG,Jones MG,Caddick MX

更新日期：2000-09-01 00:00:00

abstract：:In Saccharomyces cerevisiae MATa cells, export of the a-factor mating pheromone is mediated by Ste6p, a member of the ATP-binding cassette (ABC) superfamily of transporters and a close homologue of mammalian multidrug transporter P-glycoproteins (Pgps). We have used functional complementation of a ste6delta mutation t...

journal_title：Molecular microbiology

authors： Raymond M,Dignard D,Alarco AM,Mainville N,Magee BB,Thomas DY

更新日期：1998-02-01 00:00:00

abstract：:During pathogenesis, Mycobacterium tuberculosis (Mtb) colonizes environments, such as the macrophage or necrotic granuloma, that are acidic and rich in cholesterol and fatty acids. The goal of this study was to examine how acidic pH and available carbon sources interact to regulate Mtb physiology. Here we report that ...

journal_title：Molecular microbiology

authors： Baker JJ,Johnson BK,Abramovitch RB

更新日期：2014-10-01 00:00:00

abstract：:Candida albicans hypha formation which has been stimulated via the Ras1-cAMP-Efg1 signalling cascade is inhibited by farnesol, a C. albicans autoregulatory factor, and small molecules such as dodecanol. In cultures containing farnesol or dodecanol, hypha formation was restored upon addition of dibutyryl-cAMP. The CAI4...

journal_title：Molecular microbiology

authors： Davis-Hanna A,Piispanen AE,Stateva LI,Hogan DA

更新日期：2008-01-01 00:00:00

abstract：:Ethylenediaminetetraacetate (EDTA) is currently the most abundant organic pollutant due to its recalcitrance and extensive use. Only a few bacteria can degrade it, using EDTA monooxygenase (EmoA) to initiate the degradation. EmoA is an FMNH2 -dependent monooxygenase that requires an NADH:FMN oxidoreductase (EmoB) to p...

journal_title：Molecular microbiology

authors： Jun SY,Lewis KM,Youn B,Xun L,Kang C

更新日期：2016-06-01 00:00:00

abstract：:Escherichia coli has two heat shock regulons under the transcriptional control of Esigma32 and EsigmaE RNA polymerases. These polymerases control the expression of genes, the products of which are needed for correct folding of proteins in the cytoplasm and the extracytoplasm respectively. In this study, we report that...

journal_title：Molecular microbiology

authors： Klein G,Dartigalongue C,Raina S

更新日期：2003-04-01 00:00:00

abstract：:Fungi capable of sexual reproduction use heterothallic (self-sterile) or homothallic (self-fertile) mating strategies. In most ascomycetes, a single mating type locus, MAT, with two alternative forms (MAT1-1 and MAT1-2) called idiomorphs, controls mating ability. In heterothallic ascomycetes, these alternative idiomor...

journal_title：Molecular microbiology

authors： Lee J,Lee T,Lee YW,Yun SH,Turgeon BG

更新日期：2003-10-01 00:00:00

abstract：:Sinorhizobium meliloti is a soil bacterium that invades the root nodules it induces on Medicago sativa, whereupon it undergoes an alteration of its cell cycle and differentiates into nitrogen-fixing, elongated and polyploid bacteroid with higher membrane permeability. In Caulobacter crescentus, a related alphaproteoba...

journal_title：Molecular microbiology

authors： Pini F,Frage B,Ferri L,De Nisco NJ,Mohapatra SS,Taddei L,Fioravanti A,Dewitte F,Galardini M,Brilli M,Villeret V,Bazzicalupo M,Mengoni A,Walker GC,Becker A,Biondi EG

更新日期：2013-10-01 00:00:00

abstract：:In this paper we show that the Escherichia coli protein Fis has a regulatory function in Mu transposition in the presence of Mu repressor. Fis can lower the transposition frequency of a mini-Mu 3-80-fold, but only if the Mu repressor is expressed simultaneously. In this novel type of regulation of transposition by the...

journal_title：Molecular microbiology

authors： van Drunen CM,van Zuylen C,Mientjes EJ,Goosen N,van de Putte P

更新日期：1993-10-01 00:00:00

abstract：:The classic expression pattern of the Fis global regulatory protein during batch culture consists of a high peak in the early logarithmic phase of growth, followed by a sharp decrease through mid-exponential growth phase until Fis is almost undetectable at the end of the exponential phase. We discovered that this patt...

journal_title：Molecular microbiology

authors： O Cróinín T,Dorman CJ

更新日期：2007-10-01 00:00:00

abstract：:Bacillus subtilis responds to signals of environmental and metabolic stress by inducing over 40 general stress genes under the control of the sigma B transcription factor. sigma B activity is regulated post-translationally by a multi-component network composed of two coupled partner-switching modules, RsbX-RsbS-RsbT a...

journal_title：Molecular microbiology

authors： Akbar S,Kang CM,Gaidenko TA,Price CW

更新日期：1997-05-01 00:00:00

abstract：:The involvement of signal transduction in the repair of radiation-induced damage to DNA has been known in eukaryotes but remains understudied in bacteria. This article for the first time demonstrates a role for the periplasmic lipoprotein (YfgL) with protein kinase activity transducing a signal for DNA strand break re...

journal_title：Molecular microbiology

authors： Khairnar NP,Kamble VA,Mangoli SH,Apte SK,Misra HS

更新日期：2007-07-01 00:00:00

abstract：:In the yeast Saccharomyces cerevisiae starvation for nitrogen on a glucose-containing medium causes entrance into G0 and downregulation of all targets of the PKA pathway. Re-addition of a nitrogen source in the presence of glucose causes rapid activation of trehalase and other PKA targets. Trehalase activation upon am...

journal_title：Molecular microbiology

authors： Van Nuland A,Vandormael P,Donaton M,Alenquer M,Lourenço A,Quintino E,Versele M,Thevelein JM

更新日期：2006-03-01 00:00:00

abstract：:Toxin-antitoxin loci have been identified in almost all free-living prokaryotes, often in high copy numbers. The biological function and molecular targets of the abundant vapBC loci are not yet known. Here we analyse the vapBC loci of Salmonella LT2 and Shigella plasmid pMYSH6000. Both loci encode putative PIN (PilT N...

journal_title：Molecular microbiology

authors： Winther KS,Gerdes K

更新日期：2009-05-01 00:00:00

abstract：:The Escherichia coli dam gene and upstream sequences were cloned from the Kohara phage 4D4. Five promoters were found to contribute to dam gene transcription. P1 and P2 (the major promoter) were situated approximately 3.5 kb upstream of the structural gene, P3 was within the aroB gene, P4 was within the urf74.3 gene, ...

journal_title：Molecular microbiology

authors： Løbner-Olesen A,Boye E,Marinus MG

更新日期：1992-07-01 00:00:00

abstract：:Effector translocation is central to the virulence of many bacterial pathogens, including Streptococcus pyogenes, which utilizes the cholesterol-dependent cytolysin Streptolysin O (SLO) to translocate the NAD(+) glycohydrolase SPN into host cells during infection. SLO's translocation activity does not require host cel...

journal_title：Molecular microbiology

authors： Mozola CC,Caparon MG

更新日期：2015-09-01 00:00:00

abstract：:The effect of the unc transcription terminator on expression of uncC, encoding the epsilon subunit of Escherichia coli F1-ATPase, from plasmids was studied. The cloned sequence in pTK1 included the uncC ribosome binding site, the uncC structural gene, and the unc transcription terminator. The cloned region in pSD37 wa...

journal_title：Molecular microbiology

authors： Patel AM,Dallmann HG,Skakoon EN,Kapala TD,Dunn SD

更新日期：1990-11-01 00:00:00

abstract：:A 1.3 kb cDNA (cDNA52) was derived from Trypanosoma cruzi trypomastigote mRNA. Using single stranded probes in Northern blots, we identified the putative coding strand of cDNA52. In addition, a minor band was detected in RNA from epimastigotes that was absent in RNA from trypomastigotes. Nucleotide sequence analysis r...

journal_title：Molecular microbiology

authors： Thertulien R,Harth G,Haidaris CG

更新日期：1991-01-01 00:00:00

abstract：:Bacteria of the genera Bacillus and Clostridium form highly resistant spores, which in the case of some pathogens act as the infectious agents. An exosporium forms the outermost layer of some spores; it plays roles in protection, adhesion, dissemination, host targeting in pathogens and germination control. The exospor...

journal_title：Molecular microbiology

authors： Terry C,Jiang S,Radford DS,Wan Q,Tzokov S,Moir A,Bullough PA

更新日期：2017-05-01 00:00:00

abstract：:The endophytic fungus Epichloë festucae systemically colonizes the intercellular spaces of cool-season grasses to establish a mutualistic symbiosis. Hyphal growth of the endophyte within the host plant is tightly regulated and synchronized with the growth of the host plant. A genetic screen to identify symbiotic genes...

journal_title：Molecular microbiology

authors： Tanaka A,Kamiya S,Ozaki Y,Kameoka S,Kayano Y,Saikia S,Akano F,Uemura A,Takagi H,Terauchi R,Maruyama JI,Hammadeh HH,Fleissner A,Scott B,Takemoto D

更新日期：2020-10-01 00:00:00

abstract：:Escherichia coli RNA polymerase is able to site-specifically melt 12 bp of promoter DNA at temperatures far below those normally associated with DNA melting. Here we consider several models to explain how RNA polymerase destabilizes duplex DNA. One popular model proposes that upon binding to the promoter, RNA polymera...

journal_title：Molecular microbiology

authors： deHaseth PL,Helmann JD

更新日期：1995-06-01 00:00:00

abstract：:Two distinct merR genes, which regulate expression of the mercuric ion resistance gene (mer), of Thiobacillus ferrooxidans strain E-15 have been cloned, sequenced and termed merR1 and merR2. As a result of gene walking around two merR genes, it was found that these two genes were quite close in distance. The nucleotid...

journal_title：Molecular microbiology

authors： Inoue C,Sugawara K,Kusano T

更新日期：1991-11-01 00:00:00

abstract：:Chemoreceptors play a central role in chemotaxis, allowing bacteria to detect chemical gradients and bias their swimming behavior in order to navigate toward favorable environments. The genome of the kiwifruit pathogen, Pseudomonas syringae pv. actinidiae (Psa) strain NZ-V13 encodes 43 predicted chemoreceptors, none o...

journal_title：Molecular microbiology

authors： McKellar JL,Minnell JJ,Gerth ML

更新日期：2015-05-01 00:00:00

abstract：:A series of plasmids carrying an IRL-kan-IRR transposable cassette, in which IRL and IRR are the left- and right-terminal sequences of IS91, have been constructed. These cassettes could be complemented for transposition with similar efficiency when IS91 transposase was provided either in cis or in trans. A total of 87...

journal_title：Molecular microbiology

authors： Bernales I,Mendiola MV,de la Cruz F

更新日期：1999-07-01 00:00:00

abstract：:Cytoadherence to the vaginal epithelium is a critical step in infection by the eukaryotic flagellate Trichomonas vaginalis. Four trichomonad surface proteins (AP65, AP51, AP33 and AP23) mediate cytoadherence. The cDNA encoding the AP65 adhesin was isolated from a phagemid cDNA expression library by screening with anti...

journal_title：Molecular microbiology

authors： Alderete JF,O'Brien JL,Arroyo R,Engbring JA,Musatovova O,Lopez O,Lauriano C,Nguyen J

更新日期：1995-07-01 00:00:00

abstract：:Site-specific recombination by the Cre recombinase takes place at a simple DNA site (loxP), requires no additional proteins and gives topologically simple recombination products. In contrast, cer and psi sites for Xer recombination contain approximately 150 bp of accessory sequences, require accessory proteins PepA, A...

journal_title：Molecular microbiology

authors： Gourlay SC,Colloms SD

更新日期：2004-04-01 00:00:00

abstract：:Cellulosomes are key for lignocellulosic biomass degradation in cellulolytic Clostridia. Better understanding of the mechanism of cellulosome regulation would allow us to improve lignocellulose hydrolysis. It is hypothesized that cellulosomal protease inhibitors would regulate cellulosome architecture and then lignoce...

journal_title：Molecular microbiology

authors： Xu T,Li Y,He Z,Zhou J

更新日期：2014-02-01 00:00:00