Abstract：
:Chemoreceptors play a central role in chemotaxis, allowing bacteria to detect chemical gradients and bias their swimming behavior in order to navigate toward favorable environments. The genome of the kiwifruit pathogen, Pseudomonas syringae pv. actinidiae (Psa) strain NZ-V13 encodes 43 predicted chemoreceptors, none of which has been characterized. We developed a high-throughput fluorescence-based thermal shift assay for identifying the signal molecules that are recognized by a given chemoreceptor ligand binding domain (LBD). Using this assay, we characterized the ligand binding profiles of three Psa homologs of the P. aeruginosa PAO1 amino acid chemoreceptors PctA, PctB and PctC. Each recombinant LBD was screened against 95 potential ligands. The three Psa homologs, named pscA, pscB and pscC (Psa chemoreceptors A, B and C) bound 3, 10 and 3 amino acids respectively. In each case, their binding profiles were distinct from their P. aeruginosa PAO1 homologs. Notably, Psa PscA-LBD only bound the acidic amino acids l-aspartate, d-aspartate and l-glutamate, whereas P. aeruginosa PctA-LBD binds all of the l-proteinogenic amino acids except for l-aspartate and l-glutamate. A combination of homology modeling, site-directed mutagenesis and functional screening identified a single amino acid residue in the Psa PscA-LBD (Ala146) that is critically important for determining its narrow specificity.
journal_name
Mol Microbioljournal_title
Molecular microbiologyauthors
McKellar JL,Minnell JJ,Gerth MLdoi
10.1111/mmi.12964subject
Has Abstractpub_date
2015-05-01 00:00:00pages
694-707issue
4eissn
0950-382Xissn
1365-2958journal_volume
96pub_type
杂志文章abstract：:Differential expression of the genes expressing Pap pili in Escherichia coli was suggested to involve mRNAs with different stabilities. As the result of a post-transcriptional processing event, a papA gene-specific mRNA product (mRNA-A) accumulates in large excess relative to the primary mRNA-BA transcript. Our result...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.1991.tb01928.x
更新日期：1991-07-01 00:00:00
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pub_type： 杂志文章
doi：10.1046/j.1365-2958.2003.03867.x
更新日期：2004-02-01 00:00:00
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pub_type： 杂志文章
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更新日期：2011-05-01 00:00:00
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journal_title：Molecular microbiology
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doi：10.1046/j.1365-2958.1999.01157.x
更新日期：1999-01-01 00:00:00
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更新日期：1999-10-01 00:00:00
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pub_type： 杂志文章,评审
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更新日期：2001-09-01 00:00:00
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journal_title：Molecular microbiology
pub_type： 杂志文章,评审
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更新日期：1991-05-01 00:00:00
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更新日期：2002-11-01 00:00:00
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pub_type： 杂志文章
doi：10.1046/j.1365-2958.2001.02248.x
更新日期：2001-02-01 00:00:00
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doi：10.1111/j.1365-2958.2012.08064.x
更新日期：2012-06-01 00:00:00
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更新日期：2005-12-01 00:00:00
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pub_type： 杂志文章
doi：10.1111/j.1365-2958.1989.tb00107.x
更新日期：1989-01-01 00:00:00
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journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.2007.05724.x
更新日期：2007-05-01 00:00:00
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journal_title：Molecular microbiology
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journal_title：Molecular microbiology
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doi：10.1046/j.1365-2958.2000.01708.x
更新日期：2000-01-01 00:00:00
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journal_title：Molecular microbiology
pub_type： 杂志文章
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journal_title：Molecular microbiology
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更新日期：1994-09-01 00:00:00
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journal_title：Molecular microbiology
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更新日期：2013-08-01 00:00:00
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journal_title：Molecular microbiology
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journal_title：Molecular microbiology
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更新日期：2004-05-01 00:00:00
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pub_type： 杂志文章
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更新日期：2005-11-01 00:00:00
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journal_title：Molecular microbiology
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doi：10.1046/j.1365-2958.1998.01114.x
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