Abstract:
:Previous evidence showed that b- and a-type flagellins of Pseudomonas aeruginosa are modified in vivo by phosphorylation at tyrosine. This research was designed to demonstrate phosphorylation of flagellin at tyrosine in vitro. Evidence presented showed that flagellin is labelled by [gamma-32P]-ATP, but not by [alpha-32P]-ATP, when incubated with cell envelope fractions. Results suggested that autophosphorylation of a 42 kDa membrane protein occurred. No activity was detected in cytoplasmic fractions. Flagellin protein was identified by flagella-specific monoclonal antibody (mAb) and was labelled with anti-phosphotyrosine mAb. Confirmation of tyrosine kinase activity was shown by labelling of synthetic poly(Glu:Tyr) as a substrate with [gamma-32P]-ATP. Labelling of poly(Glu:Tyr) was heat sensitive and time dependent. Labelled phosphotyrosine was observed in partial acid hydrolysates of substrates. Using poly(Glu:Tyr) as substrate, tyrosine kinase activity was shown to be inhibited by sulphydryl reagents. It appears that tyrosine kinase and flagellin phosphorylation occur in several Pseudomonas spp. Location of phosphotyrosine in a conserved region of flagellin may serve as a cell signal so that intact flagellin is appropriately exported.
journal_name
Mol Microbioljournal_title
Molecular microbiologyauthors
South SL,Nichols R,Montie TCdoi
10.1111/j.1365-2958.1994.tb01078.xsubject
Has Abstractpub_date
1994-06-01 00:00:00pages
903-10issue
6eissn
0950-382Xissn
1365-2958journal_volume
12pub_type
杂志文章abstract::Proteins with LytM (Peptidase_M23) domains are broadly distributed in bacteria and have been implicated in a variety of important processes, including cell division and cell-shape determination. Most LytM-like proteins that have been structurally and/or biochemically characterized are metallo-endopeptidases that cleav...
journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/mmi.12304
更新日期:2013-08-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2958.2010.07335.x
更新日期:2010-10-01 00:00:00
abstract::Most of the genes required for the conjugative transfer of DNA are encoded by the 33 kb transfer (tra) operon of F-like conjugative plasmids. Transcription of the tra operon is positively regulated by the TraJ transcriptional activator which, in turn, is negatively regulated by the FinOP fertility inhibition system. T...
journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2958.1994.tb02177.x
更新日期:1994-11-01 00:00:00
abstract::A Plasmodium falciparum hexose transporter (PfHT) has previously been shown to be a facilitative glucose and fructose transporter. Its expression in Xenopus laevis oocytes and the use of a glucose analogue inhibitor permitted chemical validation of PfHT as a novel drug target. Following recent re-annotations of the P....
journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2958.2010.07060.x
更新日期:2010-03-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2958.1998.00849.x
更新日期:1998-06-01 00:00:00
abstract::Red cell invasion by Plasmodium merozoites involves multiple steps such as attachment, apical reorientation, junction formation and entry into a parasitophorous vacuole. These steps are mediated by specific molecular interactions. P. vivax and the simian parasite P. knowlesi require interaction with the Duffy blood gr...
journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2958.2005.04523.x
更新日期:2005-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:2010-06-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2958.2007.05770.x
更新日期:2007-07-01 00:00:00
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pub_type: 杂志文章
doi:10.1111/mmi.12898
更新日期:2015-03-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2958.1993.tb00948.x
更新日期:1993-11-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/mmi.14575
更新日期:2020-10-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2958.1995.tb02397.x
更新日期:1995-04-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2958.1993.tb00901.x
更新日期:1993-10-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2958.1996.00111.x
更新日期:1996-10-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2958.2002.02768.x
更新日期:2002-02-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2958.2005.04912.x
更新日期:2005-12-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2958.2012.08064.x
更新日期:2012-06-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2958.2008.06165.x
更新日期:2008-04-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/mmi.13629
更新日期:2017-04-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章,评审
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更新日期:1998-10-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:2001-11-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2958.1996.00074.x
更新日期:1996-09-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/mmi.13147
更新日期:2015-11-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章,评审
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更新日期:2012-10-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2958.1999.01245.x
更新日期:1999-02-01 00:00:00
abstract::The ubiquitous human commensal Escherichia coli has been well investigated through its model representative E. coli K-12. In this work, we initially characterized E. coli Fec10, a recently isolated human commensal strain of phylogroup A/sequence type ST10. Compared to E. coli K-12, the 4.88 Mbp Fec10 genome is charact...
journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/mmi.14614
更新日期:2020-09-28 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2958.2010.07180.x
更新日期:2010-06-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2958.1996.386918.x
更新日期:1996-02-01 00:00:00
abstract::Transcript degradation is a key step in gene regulation. In eukaryotes, mRNA decay is generally initiated by removal of the poly(A) tail mediated by the Ccr4-Caf1-Not complex. Deadenylated transcripts are then rapidly degraded, primarily via the decapping-dependent pathway. Components of this pathway can be localized ...
journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2958.2010.07118.x
更新日期:2010-04-01 00:00:00
abstract::A growing class of proteins regulates transcription through interaction with DNA-dependent RNA polymerase. Here we report that a recently identified, highly conserved sporulation gene ylyA encodes a novel RNA polymerase-binding protein that influences the expression of genes under the control of the late-acting, sporu...
journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/mmi.12262
更新日期:2013-07-01 00:00:00