Entamoeba histolytica and Entamoeba dispar: differences in numbers and expression of cysteine proteinase genes.

abstract：:The genetic organization of the spoVAF-serA area of the Bacillus subtilis chromosome and its putative transcription map have been derived from analysis of the nucleotide sequence. In order to confirm this transcription map as regards size of transcripts and to determine growth conditions for their appearance, we under...

journal_title：Molecular microbiology

authors： Azevedo V,Sorokin A,Ehrlich SD,Serror P

更新日期：1993-10-01 00:00:00

abstract：:Colonies of strains carrying a stable lambdaplacMu15 translational fusion displayed sharply defined intense staining at the centre on Xgal medium. The fusion was in fiu (ferric ion uptake), encoding an iron-regulated outer membrane protein (IROMP) controlled via four overlapping ferric uptake regulator (Fur) boxes in ...

journal_title：Molecular microbiology

authors： Newman DL,Shapiro JA

更新日期：1999-07-01 00:00:00

abstract：:Although diminutive in size, bacteria possess highly diverse and spatially confined cellular structures. Two related alphaproteobacteria, Sinorhizobium meliloti and Caulobacter crescentus, serve as models for investigating the genetic basis of morphological variations. S. meliloti, a symbiont of leguminous plants, syn...

journal_title：Molecular microbiology

authors： Fields AT,Navarrete CS,Zare AZ,Huang Z,Mostafavi M,Lewis JC,Rezaeihaghighi Y,Brezler BJ,Ray S,Rizzacasa AL,Barnett MJ,Long SR,Chen EJ,Chen JC

更新日期：2012-06-01 00:00:00

abstract：:In Escherichia coli, after DNA damage, the SOS response increases the transcription (and protein levels) of approximately 50 genes. As DNA repair ensues, the level of transcription returns to homeostatic levels. ClpXP and other proteases return the high levels of several SOS proteins to homeostasis. When all SOS genes...

journal_title：Molecular microbiology

authors： Warr AR,Klimova AN,Nwaobasi AN,Sandler SJ

更新日期：2019-02-01 00:00:00

abstract：:Two operons, comAB and comCDE, play a key role in the co-ordination of spontaneous competence development in cultures of Streptococcus pneumoniae. ComAB is required for export of the comC-encoded competence-stimulating peptide (CSP). Upon CSP binding, the histidine kinase ComD activates ComE, its cognate response regu...

journal_title：Molecular microbiology

authors： Martin B,Prudhomme M,Alloing G,Granadel C,Claverys JP

更新日期：2000-11-01 00:00:00

abstract：:The ferric uptake regulator (Fur) protein is known to act as a Fe2+-dependent transcriptional repressor of bacterial promoters. Here, we show that, in Helicobacter pylori, Fur can mediate the regulation of iron-activated genes in contrast to classical Fur regulation, in which iron acts as a co-repressor. Inactivation ...

journal_title：Molecular microbiology

authors： Delany I,Spohn G,Rappuoli R,Scarlato V

更新日期：2001-12-01 00:00:00

abstract：:The analysis of completed archaeal genome sequences led to the identification of a set of approximately 10-20 genes whose protein products were inferred to be involved in chromosomal DNA replication. Until recently, however, little was known of the biochemical properties of these proteins. Here, I review recent progre...

journal_title：Molecular microbiology

authors： MacNeill SA

更新日期：2001-05-01 00:00:00

abstract：:Protein kinase G of Mycobacterium tuberculosis has been implicated in virulence and in regulation of glutamate metabolism. Here we show that this kinase undergoes a pattern of autophosphorylation that is distinct from that of other M. tuberculosis protein kinases characterized to date and we identify GarA as a substra...

journal_title：Molecular microbiology

authors： O'Hare HM,Durán R,Cerveñansky C,Bellinzoni M,Wehenkel AM,Pritsch O,Obal G,Baumgartner J,Vialaret J,Johnsson K,Alzari PM

更新日期：2008-12-01 00:00:00

abstract：:A hallmark of the biofilm architecture is the presence of microcolonies. However, little is known about the underlying mechanisms governing microcolony formation. In the pathogen Pseudomonas aeruginosa, microcolony formation is dependent on the two-component regulator MifR, with mifR mutant biofilms exhibiting an over...

journal_title：Molecular microbiology

authors： Petrova OE,Schurr JR,Schurr MJ,Sauer K

更新日期：2012-11-01 00:00:00

abstract：:Initiation and re-initiation of chromosomal DNA replication in bacteria rely on divergent multiprotein assemblies, which direct the functional delivery of the replicative helicase on single-stranded DNA (ssDNA) at specific sites. These two processes are triggered either at the single chromosomal origin oriC or at arre...

journal_title：Molecular microbiology

authors： Bruand C,Velten M,McGovern S,Marsin S,Sérèna C,Ehrlich SD,Polard P

更新日期：2005-02-01 00:00:00

abstract：:The cytokinetic apparatus of bacteria is initially formed by the polymerization of the tubulin-like FtsZ protein into a ring structure at midcell. This so-called Z-ring facilitates the recruitment of many additional proteins to the division site to form the mature divisome machine. Although the assembly pathway leadin...

journal_title：Molecular microbiology

authors： Tsang MJ,Bernhardt TG

更新日期：2015-03-01 00:00:00

abstract：:Copy-number mutants of Staphylococcus aureus macrolide-lincosamide-streptogramin B (MLS) resistance plasmid pT48 were isolated by their resistance to the non-inducing macrolide, tylosin. One mutant plasmid, pcopD3, showed a three- to five-fold cis-dominant increase in copy number, and nucleotide sequence analysis reve...

journal_title：Molecular microbiology

authors： Catchpole I,Dyke KG

更新日期：1991-04-01 00:00:00

abstract：:The suf and isc operons of Escherichia coli have been implicated in Fe-S cluster assembly. However, it has been unclear why E. coli has two systems for Fe-S cluster biosynthesis. We have examined the regulatory characteristics and mutant phenotypes of both operons to discern if the two operons have redundant functions...

journal_title：Molecular microbiology

authors： Outten FW,Djaman O,Storz G

更新日期：2004-05-01 00:00:00

abstract：:A PCR-amplified DNA fragment of the relA gene from genomic Bacillus subtilis DNA was used to isolate the entire relA/spoT homologue and two adjacent open reading frames (ORFs) from a lambda ZAP Express library. The relA gene, which encodes a protein of 734 amino acid residues (aa), is flanked by an ORF (170aa) that sh...

journal_title：Molecular microbiology

authors： Wendrich TM,Marahiel MA

更新日期：1997-10-01 00:00:00

abstract：:The DNA repeat region of fcrA76, the gene encoding a group A streptococcal Fc-binding protein, was subcloned in-frame into an Escherichia coli plasmid expression vector. The expressed protein product displayed the same Fc-binding properties as the full-length Fc-binding protein expressed from fcrA76. The affinity-puri...

journal_title：Molecular microbiology

authors： Heath DG,Boyle MD,Cleary PP

更新日期：1990-12-01 00:00:00

abstract：:Fungi capable of sexual reproduction use heterothallic (self-sterile) or homothallic (self-fertile) mating strategies. In most ascomycetes, a single mating type locus, MAT, with two alternative forms (MAT1-1 and MAT1-2) called idiomorphs, controls mating ability. In heterothallic ascomycetes, these alternative idiomor...

journal_title：Molecular microbiology

authors： Lee J,Lee T,Lee YW,Yun SH,Turgeon BG

更新日期：2003-10-01 00:00:00

abstract：:Agrobacterium tumefaciens uses a type IV secretion (T4S) system composed of VirB proteins and VirD4 to deliver oncogenic DNA (T-DNA) and protein substrates to susceptible plant cells during the course of infection. Here, by use of the Transfer DNA ImmunoPrecipitation (TrIP) assay, we present evidence that the mobiliza...

journal_title：Molecular microbiology

authors： Cascales E,Atmakuri K,Liu Z,Binns AN,Christie PJ

更新日期：2005-10-01 00:00:00

abstract：:A series of internal deletions in the lktA gene of Pasteurella haemolytica has been constructed. All of the deletions eliminated the lytic activity of the leukotoxin towards the bovine lymphoma cell line, BL-3. Deletions removing segments of the amino-proximal hydrophobic region, which is thought to constitute an esse...

journal_title：Molecular microbiology

authors： Cruz WT,Young R,Chang YF,Struck DK

更新日期：1990-11-01 00:00:00

abstract：:A method of insertional mutagenesis for naturally transformable organisms has been adapted from Haemophilus influenzae and applied to the study of the pathogenesis of Campylobacter jejuni. A series of kanamycin-resistant insertional mutants of C. jejuni 81-176 has been generated and screened for loss of ability to inv...

journal_title：Molecular microbiology

authors： Yao R,Burr DH,Doig P,Trust TJ,Niu H,Guerry P

更新日期：1994-12-01 00:00:00

abstract：:Experimental evidence suggests that apicomplexan parasites possess bipartite promoters with basal and regulated cis-elements similar to other eukaryotes. Using a dual luciferase model adapted for recombinational cloning and use in Toxoplasma gondii, we show that genomic regions flanking 16 parasite genes, which encomp...

journal_title：Molecular microbiology

authors： Behnke MS,Radke JB,Smith AT,Sullivan WJ Jr,White MW

更新日期：2008-06-01 00:00:00

abstract：:In matings between Lactococcus lactis strains, the conjugative transposons Tn916 and Tn919 are found in the chromosome of the transconjugants in the same place as in the chromosome of the donor, indicating that no transposition has occurred. In agreement with this, the frequency of L. lactis transconjugants from intra...

journal_title：Molecular microbiology

authors： Bringel F,Van Alstine GL,Scott JR

更新日期：1991-12-01 00:00:00

abstract：:The DNA damage and stress response pathways interact to regulate cellular responses to genotoxins and environmental stresses. How these pathways interact in Schizosaccharomyces pombe is not well understood. We demonstrate that osmotic stress suppresses the DNA damage sensitivity of checkpoint mutants, and that this oc...

journal_title：Molecular microbiology

authors： Alao JP,Huis In 't Veld PJ,Buhse F,Sunnerhagen P

更新日期：2010-07-01 00:00:00

abstract：:Yersinia enterocolitica uses type III secretion to transport Yop proteins into the cytoplasm of host cells. Previous work generated hypotheses for both co- and post-translational transport mechanisms in the Yersinia type III pathway. Here, we used ubiquitin (Ub) and UBP1, the Ub-specific protease, to examine whether Y...

journal_title：Molecular microbiology

authors： Quenee LE,Schneewind O

更新日期：2007-07-01 00:00:00

abstract：:Visual inspection showed clear evidence of a history of intraspecies recombinational exchanges within the neighbouring meningococcal shikimate dehydrogenase (aroE) and glutamine synthetase (glnA) genes, which was supported by the non-congruence of the trees constructed from the sequences of these genes from different ...

journal_title：Molecular microbiology

authors： Zhou J,Bowler LD,Spratt BG

更新日期：1997-02-01 00:00:00

abstract：:Bacterial cell shapes are manifestations of programs carried out by multi-protein machines that synthesize and remodel the resilient peptidoglycan (PG) mesh and other polymers surrounding cells. GpsB protein is conserved in low-GC Gram-positive bacteria and is not essential in rod-shaped Bacillus subtilis, where it pl...

journal_title：Molecular microbiology

authors： Land AD,Tsui HC,Kocaoglu O,Vella SA,Shaw SL,Keen SK,Sham LT,Carlson EE,Winkler ME

更新日期：2013-12-01 00:00:00

abstract：:In F. graminearum, the transcription factor TRI6 positively regulates the trichothecene biosynthetic gene cluster (BGC) leading to the production of the secondary metabolite 15-acetyl deoxynivalenol. Secondary metabolites are not essential for survival, instead, they enable the pathogen to successfully infect its host...

journal_title：Molecular microbiology

authors： Shostak K,Bonner C,Sproule A,Thapa I,Shields SWJ,Blackwell B,Vierula J,Overy D,Subramaniam R

更新日期：2020-10-01 00:00:00

abstract：:In the chemotaxis of Escherichia coli, adaptation requires the methylation and demethylation of transmembrane receptors, which are catalysed by the methyltransferase CheR and the methylesterase CheB respectively. CheR binds to major chemoreceptors through their C-terminal motif NWETF, which is distinct from the methyl...

journal_title：Molecular microbiology

authors： Shiomi D,Okumura H,Homma M,Kawagishi I

更新日期：2000-04-01 00:00:00

abstract：:Maturation of c-type cytochromes in Escherichia coli is a complex process requiring eight membrane proteins encoded by the ccmABCDEFGH operon. CcmE is a mediator of haem delivery. It binds haem transiently at a conserved histidine residue and releases it for directed transfer to apocytochrome c. CcmC, an integral memb...

journal_title：Molecular microbiology

authors： Schulz H,Pellicioli EC,Thöny-Meyer L

更新日期：2000-09-01 00:00:00

abstract：:The td group I intron interrupting the thymidylate synthase (TS) gene of phage T4 is a mobile intron that encodes the homing endonuclease I-TevI. Efficient RNA splicing of the intron is required to restore function of the TS gene, while expression of I-TevI from within the intron is required to initiate intron mobilit...

journal_title：Molecular microbiology

authors： Gibb EA,Edgell DR

更新日期：2010-10-01 00:00:00

abstract：:Establishment of an axis of cell polarity and differentiation of the cell poles are fundamental aspects of cellular development in many organisms. We compared the effects of two bacterial cytoskeletal-like systems, the MreB and MinCDE systems, on these processes in Escherichia coli. We report that the Min proteins are...

journal_title：Molecular microbiology

authors： Shih YL,Kawagishi I,Rothfield L

更新日期：2005-11-01 00:00:00