Abstract:
:A PCR-amplified DNA fragment of the relA gene from genomic Bacillus subtilis DNA was used to isolate the entire relA/spoT homologue and two adjacent open reading frames (ORFs) from a lambda ZAP Express library. The relA gene, which encodes a protein of 734 amino acid residues (aa), is flanked by an ORF (170aa) that shares high similarity to adenine phosphoribosyltransferase genes (apt), and downstream by an ORF (131 aa) of unknown function. This genetic organization is similar to that in Streptomyces coelicolor A3(2) and Streptococcus equismilis H46A. relA shows significant similarity to the Escherichia coli relA and spoT genes, which are responsible for the synthesis and degradation of the highly phosphorylated guanosine nucleotides (p)ppGpp, triggering the stringent response. Deletion of the relA gene generated a (p)ppGpp0 phenotype that demonstrated its essential role in the response to amino acid deprivation and resulted in impaired/lowered induction of proteins involved in stress response as well as amino acid biosynthesis, as judged by two-dimensional gel electrophoresis. The same effects of impaired induction of some sigmaB-independent proteins could also be shown in a sigB/relA double mutant, supporting the role of relA in derepression/induction of catabolic and anabolic genes during stringent response.
journal_name
Mol Microbioljournal_title
Molecular microbiologyauthors
Wendrich TM,Marahiel MAdoi
10.1046/j.1365-2958.1997.5511919.xsubject
Has Abstractpub_date
1997-10-01 00:00:00pages
65-79issue
1eissn
0950-382Xissn
1365-2958journal_volume
26pub_type
杂志文章abstract::Recent studies examining the molecular mechanisms of isoniazid (INH) resistance in Mycobacterium tuberculosis have demonstrated that a significant percentage of drug-resistant strains are mutated in the katG gene which encodes a catalase-peroxidase, and the majority of these alterations are missense mutations which re...
journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2958.1996.00133.x
更新日期:1996-11-01 00:00:00
abstract::The AMA1 sequence is an efficient plasmid replicator and transformation enhancer in Aspergillus nidulans. It comprises two long perfect inverted repeats (MATE elements) flanking a short, unique, central spacer. Subclone analysis indicates that the complete inverted duplication, but not the unique central spacer, is ne...
journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2958.1996.tb02629.x
更新日期:1996-04-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2958.2002.02956.x
更新日期:2002-06-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2958.2005.05010.x
更新日期:2006-02-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:1991-03-01 00:00:00
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pub_type: 杂志文章
doi:10.1046/j.1365-2958.2003.03768.x
更新日期:2003-11-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 评论,杂志文章
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更新日期:2011-04-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/mmi.12749
更新日期:2014-10-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章,评审
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更新日期:1989-11-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:2010-10-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/mmi.12304
更新日期:2013-08-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/mmi.12067
更新日期:2012-12-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2958.2010.07518.x
更新日期:2011-03-01 00:00:00
abstract::The overall transcription of DNA has previously been demonstrated to proceed at extremely high levels of accuracy. We review the evidence that the process of transcription is subject to proof-reading in the Hopfield sense. In addition, we speculate that the proof-reading activity associated with transcription is subje...
journal_title:Molecular microbiology
pub_type: 杂志文章,评审
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更新日期:1991-05-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2958.2000.01679.x
更新日期:2000-01-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2958.1999.01477.x
更新日期:1999-07-01 00:00:00
abstract::The partition system of the P1 plasmid, P1par, consists of the ParA and ParB proteins and a cis-acting site, parS. It is responsible for the orderly segregation of plasmid copies to daughter cells. Plasmids with null mutations in parA or parB replicate normally, but missegregate. ParB binds specifically to the parS si...
journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2958.1997.4761842.x
更新日期:1997-09-01 00:00:00
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pub_type: 杂志文章
doi:10.1046/j.1365-2958.2002.03119.x
更新日期:2002-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:2007-11-01 00:00:00
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journal_title:Molecular microbiology
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journal_title:Molecular microbiology
pub_type: 杂志文章,评审
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更新日期:2011-07-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章,评审
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更新日期:1990-01-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/mmi.14078
更新日期:2018-09-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:2001-11-01 00:00:00
abstract::Alternative sigma (σ) factors govern expression of bacterial genes in response to diverse environmental signals. In Pseudomonas aeruginosa σPvdS directs expression of genes for production of a siderophore, pyoverdine, as well as a toxin and a protease. σFpvI directs expression of a receptor for ferripyoverdine import....
journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/mmi.13855
更新日期:2017-12-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:2005-02-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:2005-03-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:1993-12-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2958.1994.tb00484.x
更新日期:1994-09-01 00:00:00
abstract::Using a previously developed expression system based on the erythromycin-producing strain of Saccharopolyspora erythraea, O-methyltransferases from the spinosyn biosynthetic gene cluster of Saccharopolyspora spinosa have been shown to modify a rhamnosyl sugar attached to a 14-membered polyketide macrolactone. The spnI...
journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2958.2001.02594.x
更新日期:2001-09-01 00:00:00