Families of bacterial signal-transducing proteins.

abstract：:We have used a targeted gene deletion event to remove the coding region for the bgl1 gene encoding an extracellular beta-glucosidase from the genome of the cellulolytic fungus Trichoderma reesei. The bgl1 null mutants were used to investigate the role of beta-glucosidase in the hydrolysis of cellulose and induction of...

journal_title：Molecular microbiology

authors： Fowler T,Brown RD Jr

更新日期：1992-11-01 00:00:00

abstract：:Carbapenems such as meropenem are being investigated for their potential therapeutic utility against highly drug-resistant tuberculosis. These β-lactams target the transpeptidases that introduce interpeptide cross-links into bacterial peptidoglycan thereby controlling rigidity of the bacterial envelope. Treatment of M...

journal_title：Molecular microbiology

authors： Kumar P,Arora K,Lloyd JR,Lee IY,Nair V,Fischer E,Boshoff HI,Barry CE 3rd

更新日期：2012-10-01 00:00:00

abstract：:RecA2278-5 is a mutant RecA protein (RecAmut) bearing two amino acid substitutions, Gly-278 to Thr and Val-275 to Phe, in the alpha-helix H of the C-terminal subdomain of the protein. RecA2278-5 mutant cells are unusual in that they are thermosensitive for recombination but almost normal for DNA repair of UV damage an...

journal_title：Molecular microbiology

authors： Alexseyev AA,Baitin DM,Kuramitsu S,Ogawa T,Ogawa H,Lanzov VA

更新日期：1997-01-01 00:00:00

abstract：:Several Fusarium species produce the polyketide mycotoxin zearalenone (ZEA), a causative agent of hyperestrogenic syndrome in animals that is often found in F. graminearum-infected cereals in temperate regions. The ZEA biosynthetic cluster genes PKS4, PKS13, ZEB1 and ZEB2 encode a reducing polyketide synthase, a non-r...

journal_title：Molecular microbiology

authors： Park AR,Son H,Min K,Park J,Goo JH,Rhee S,Chae SK,Lee YW

更新日期：2015-09-01 00:00:00

abstract：:Sequence-inherent targeting information directs polypeptides synthesized in the cytosol to their respective cellular compartment. Some proteins use ambiguous sorting signals or specific folding properties to be dually distributed between the cytosol and mitochondria. A study published in this issue of Molecular Microb...

journal_title：Molecular microbiology

authors： Herrmann JM

更新日期：2009-04-01 00:00:00

abstract：:Phaseolotoxin, a phytotoxin of Pseudomonas syringae pv. phaseolicola, is produced at 18 degrees C but not at 28 degrees C. Here we report that a fragment (24.4 kb) cloned from the wild-type strain, which does not harbour a gene(s) involved in phaseolotoxin biosynthesis, abolishes this thermoregulation in the wild type...

journal_title：Molecular microbiology

authors： Rowley KB,Clements DE,Mandel M,Humphreys T,Patil SS

更新日期：1993-05-01 00:00:00

abstract：:The molecular mechanisms underpinning switching of variant antigens on the surface of Plasmodium falciparum-infected erythrocytes are poorly understood. We tested the hypothesis that insertional disruption of the A4var gene, one of two var genes located within the subtelomeric region of one end of chromosome 13, would...

journal_title：Molecular microbiology

authors： Horrocks P,Pinches R,Kyes S,Kriek N,Lee S,Christodoulou Z,Newbold CI

更新日期：2002-08-01 00:00:00

abstract：:The phsA gene encodes phenoxazinone synthase (PHS), which catalyses the penultimate step in the pathway for actinomycin biosynthesis in Streptomyces antibioticus. The phsA promoter strikingly resembles a putative Streptomyces sigma E cognate promoter, and purified E sigma E holoenzyme transcribed the phsA promoter in ...

journal_title：Molecular microbiology

authors： Jones GH,Paget MS,Chamberlin L,Buttner MJ

更新日期：1997-01-01 00:00:00

abstract：:The minimum time (E) required for a new pair of replication origins (oriCs) produced upon initiating a round of replication to be ready to initiate the next round after one cell mass doubling, the 'eclipse', is explained in terms of a minimal distance (l(min)) that the replication forks must move away from oriC before...

journal_title：Molecular microbiology

authors： Zaritsky A,Vischer N,Rabinovitch A

更新日期：2007-01-01 00:00:00

abstract：:Bacillus subtilis SpoIIE is a Ser protein phosphatase whose action on the phosphoprotein SpoIIAA triggers the cell type-specific activation of a sporulation transcription factor. Here we report that SpoIIE displays sequence similarity to the PP2C family of eukaryotic Ser/Thr protein phosphatases, and that residues com...

journal_title：Molecular microbiology

authors： Adler E,Donella-Deana A,Arigoni F,Pinna LA,Stragler P

更新日期：1997-01-01 00:00:00

abstract：:The ability of Salmonella typhimurium to survive and replicate within murine macrophages is dependent on a low phagosomal pH. This requirement for an acidic vacuole suggests that low pH is an important environmental stimulus for the transcription of genes necessary for intracellular survival. To study the behaviour of...

journal_title：Molecular microbiology

authors： Valdivia RH,Falkow S

更新日期：1996-10-01 00:00:00

abstract：:Mechanisms of hydrogen peroxide generation in Escherichia coli were investigated using a strain lacking scavenging enzymes. Surprisingly, the deletion of many abundant flavoenzymes that are known to autoxidize in vitro did not substantially lessen overall H(2)O(2) formation. However, H(2)O(2) production diminished by ...

journal_title：Molecular microbiology

authors： Korshunov S,Imlay JA

更新日期：2010-03-01 00:00:00

abstract：:9 alpha-Hydroxylation of 4-androstene-3,17-dione (AD) and 1,4-androstadiene-3,17-dione (ADD) is catalysed by 3-ketosteroid 9 alpha-hydroxylase (KSH), a key enzyme in microbial steroid catabolism. Very limited knowledge is presently available on the KSH enzyme. Here, we report for the first time the identification and ...

journal_title：Molecular microbiology

authors： van der Geize R,Hessels GI,van Gerwen R,van der Meijden P,Dijkhuizen L

更新日期：2002-08-01 00:00:00

abstract：:Nitrogen metabolism genes of Bacillus subtilis are regulated by the availability of rapidly metabolizable nitrogen sources, but not by any mechanism analogous to the two-component Ntr regulatory system found in enteric bacteria. Instead, at least three regulatory proteins independently control the expression of gene p...

journal_title：Molecular microbiology

authors： Fisher SH

更新日期：1999-04-01 00:00:00

abstract：:Encased within the 280 kb genome in the capsid of the giant myovirus φKZ is an unusual cylindrical proteinaceous 'inner body' of highly ordered structure. We present here mass spectrometry, bioinformatic and biochemical studies that reveal novel information about the φKZ head and the complex inner body. The identifica...

journal_title：Molecular microbiology

authors： Thomas JA,Weintraub ST,Wu W,Winkler DC,Cheng N,Steven AC,Black LW

更新日期：2012-04-01 00:00:00

abstract：:The nucleotide sequence of a 2711bp DNA segment which contains the N-terminal coding sequence and the 5' flanking region of a crystal protein gene (bta) from Bacillus thuringiensis subsp. aizawai 7.29 has been determined. The coding region encodes an 824 amino-acid polypeptide corresponding to a carboxy-terminally tru...

journal_title：Molecular microbiology

authors： Sanchis V,Lereclus D,Menou G,Chaufaux J,Guo S,Lecadet MM

更新日期：1989-02-01 00:00:00

abstract：:The osteolytic toxin of Pasteurella multocida induces bone resorption in vivo and in vitro (Foged et al., 1988; Kimman et al., 1987). In this report the toxin-encoding toxA gene is sequenced, and the deduced primary structure of the toxin shows a protein of 1285 amino acids, containing a striking homology to a metal-b...

journal_title：Molecular microbiology

authors： Petersen SK

更新日期：1990-05-01 00:00:00

abstract：:In response to genotoxic stress (GS), Candida albicans can undergo polarized growth and massive genome rearrangements including loss-of-heterozygosity (LOH) events. We evaluated the contribution of the CaRad53p and CaDun1p kinases of the DNA damage checkpoint (DDCP) in these processes. Characterization of C. albicans ...

journal_title：Molecular microbiology

authors： Loll-Krippleber R,d'Enfert C,Feri A,Diogo D,Perin A,Marcet-Houben M,Bougnoux ME,Legrand M

更新日期：2014-02-01 00:00:00

abstract：:In contrast to most primary metabolism genes, the genes involved in secondary metabolism and certain nutrient utilization pathways are clustered in fungi. Recently a nuclear protein, LaeA, was found to be required for the transcription of several secondary metabolite gene clusters in Aspergillus nidulans. Here we show...

journal_title：Molecular microbiology

authors： Bok JW,Noordermeer D,Kale SP,Keller NP

更新日期：2006-09-01 00:00:00

abstract：:Two pathogenic species within the genus Neisseria cause the diseases gonorrhoea and meningitis. While vaccines are available to protect against four N. meningitidis serogroups, there is currently no commercial vaccine to protect against serogroup B or against N. gonorrhoeae. Moreover, the available vaccines have signi...

journal_title：Molecular microbiology

authors： Noinaj N,Buchanan SK,Cornelissen CN

更新日期：2012-10-01 00:00:00

abstract：:Expression of the cysteine regulon in Salmonella typhimurium and Escherichia coli is controlled by the LysR-type transcriptional activator CysB and by the inducer N-acetyl-L-serine. Sulphide and thiosulphate are anti-inducers. Two highly purified constitutive CysB proteins, CysB(T149M) and CysB(T149P), were found to b...

journal_title：Molecular microbiology

authors： Colyer TE,Kredich NM

更新日期：1996-07-01 00:00:00

abstract：:In response to nitrate availability, Escherichia coli regulates the synthesis of a number of enzymes involved in anaerobic respiration and fermentation. When nitrate is present, nitrate reductase (narGHJI) gene expression is induced, while expression of the DMSO/TMAO reductase (dmsABC), fumarate reductase (frdABCD) an...

journal_title：Molecular microbiology

authors： Chiang RC,Cavicchioli R,Gunsalus RP

更新日期：1992-07-01 00:00:00

abstract：:Cytochrome c biogenesis describes the posttranslational pathway for the conversion of pre-apocytochrome c into the mature holocytochrome c. It involves an unknown number of consecutive biochemical steps, including translocation of the precursor polypeptide and haem into the periplasm and the covalent linkage between t...

journal_title：Molecular microbiology

authors： Thöny-Meyer L,Ritz D,Hennecke H

更新日期：1994-04-01 00:00:00

abstract：:A search with the proposed amino acid translation product from the new 'candidate gene' for human Menkes disease against protein sequence libraries showed a remarkable similarity to that for the cadmium efflux ATPase from Staphylococcus aureus resistance plasmids. The Menkes sequence appears closer to the CadA Cd2+ se...

journal_title：Molecular microbiology

authors： Silver S,Nucifora G,Phung LT

更新日期：1993-10-01 00:00:00

abstract：:RbpA is an RNA polymerase-binding protein that occurs in the actinomycete family of bacteria and is regulated by the disulphide stress-response sigma factor, sigma(R), in Streptomyces coelicolor. Here we demonstrate that rbpA null mutants exhibit a slow-growth phenotype and are particularly sensitive to the transcript...

journal_title：Molecular microbiology

authors： Newell KV,Thomas DP,Brekasis D,Paget MS

更新日期：2006-05-01 00:00:00

abstract：:Eeyarestatin 1 (ES1) is an inhibitor of endoplasmic reticulum (ER) associated protein degradation, Sec61-dependent Ca2+ homeostasis and protein translocation into the ER. Recently, evidence was presented showing that a smaller analog of ES1, ES24, targets the Sec61-translocon, and captures it in an open conformation t...

journal_title：Molecular microbiology

authors： Steenhuis M,Koningstein GM,Oswald J,Pick T,O'Keefe S,Koch HG,Cavalié A,Whitehead RC,Swanton E,High S,Luirink J

更新日期：2020-08-15 00:00:00

abstract：:The pulmonary pathogen Legionella pneumophila uses the Dot/Icm type IV secretion system (T4SS) to replicate inside host cells. This apparatus translocates proteins into macrophages to alter their endocytic pathway and enable bacterial growth. Although the secretion ATPase DotB is critical for T4SS function, its specif...

journal_title：Molecular microbiology

authors： Sexton JA,Yeo HJ,Vogel JP

更新日期：2005-07-01 00:00:00

abstract：:The Dot/Icm system is a type IVb secretion system used by Legionella pneumophila to modulate vesicular transport in both protozoan and mammalian host cells. It has been shown that proteins and processes that are highly conserved in all eukaryotic cells are targets for some of the proteins injected by the Dot/Icm syste...

journal_title：Molecular microbiology

authors： Campodonico EM,Chesnel L,Roy CR

更新日期：2005-05-01 00:00:00

abstract：:Erwinia chrysanthemi, a phytopathogenic bacterium, produces a protease inhibitor which is a low-molecular-weight, heat-stable protein. In addition to its action on the three E. chrysanthemi extracellular proteases A, B and C, it also strongly inhibits the 50 kD extracellular protease of Serratia marcescens. Its struct...

journal_title：Molecular microbiology

authors： Létoffé S,Delepelaire P,Wandersman C

更新日期：1989-01-01 00:00:00

abstract：:Proteinaceous, hair-like appendages known as fimbriae or pili commonly extend from the surface of prokaryotic cells and serve important functions such as cell adhesion, biofilm formation, motility and DNA transfer. Here we show that a novel group of archaea from cold, sulphidic springs has developed cell surface appen...

journal_title：Molecular microbiology

authors： Moissl C,Rachel R,Briegel A,Engelhardt H,Huber R

更新日期：2005-04-01 00:00:00