Sigma-E is required for the production of the antibiotic actinomycin in Streptomyces antibioticus.

abstract：:The Vipp1 protein is essential in cyanobacteria and chloroplasts for the maintenance of photosynthetic function and thylakoid membrane architecture. To investigate its mode of action we generated strains of the cyanobacteria Synechocystis sp. PCC6803 and Synechococcus sp. PCC7942 in which Vipp1 was tagged with green f...

journal_title：Molecular microbiology

authors： Bryan SJ,Burroughs NJ,Shevela D,Yu J,Rupprecht E,Liu LN,Mastroianni G,Xue Q,Llorente-Garcia I,Leake MC,Eichacker LA,Schneider D,Nixon PJ,Mullineaux CW

更新日期：2014-10-13 00:00:00

abstract：:Photoregulation of pigmentation during complementary chromatic acclimation (CCA) is well studied in Fremyella diplosiphon; however, mechanistic insights into the CCA-associated morphological changes are still emerging. F. diplosiphon cells are rectangular under green light (GL), whereas cells are smaller and spherical...

journal_title：Molecular microbiology

authors： Singh SP,Montgomery BL

更新日期：2014-07-01 00:00:00

abstract：:The mobile insertion sequence, IS6110, is an important marker in tracking of Mycobacterium tuberculosis strains. Here, we demonstrate that IS6110 can upregulate downstream genes through an outward-directed promoter in its 3' end, thus adding to the significance of this element. Promoter activity was orientation depend...

journal_title：Molecular microbiology

authors： Safi H,Barnes PF,Lakey DL,Shams H,Samten B,Vankayalapati R,Howard ST

更新日期：2004-05-01 00:00:00

abstract：:When Escherichia coli is exposed to redox-cycling drugs, its SoxR transcription factor is activated by oxidation of its [2Fe-2S] cluster. In aerobic cells these drugs generate superoxide, and because superoxide dismutase (SOD) is a member of the SoxRS regulon, superoxide was initially thought to be the activator of So...

journal_title：Molecular microbiology

authors： Gu M,Imlay JA

更新日期：2011-03-01 00:00:00

abstract：UNLABELLED:Haem Nitric oxide/OXygen (H-NOX) binding domains are a family of haemoprotein sensors that are widespread in bacterial genomes, but limited information is available on their function. Legionella pneumophila is the only prokaryote found, thus far, to encode two H-NOX proteins. This paper presents data support...

journal_title：Molecular microbiology

authors： Carlson HK,Vance RE,Marletta MA

更新日期：2010-08-01 00:00:00

abstract：:Strains of Escherichia coli have been produced which express very high levels of the tRNA(1Leu) isoacceptor. This was accomplished by transforming cells with plasmids containing the leuV operon which encodes three copies of the tRNA(1Leu) gene. Most transformants grew very slowly and exhibited a 15-fold increase in ce...

journal_title：Molecular microbiology

authors： Wahab SZ,Rowley KO,Holmes WM

更新日期：1993-01-01 00:00:00

abstract：:N-terminal protein acetylation is common in eukaryotes and halophilic archaea, but very rare in bacteria. We demonstrate that some of the most abundant proteins present in the crenarchaeote Sulfolobus solfataricus, including subunits of the thermosome, proteosome and ribosome, are acetylated at the N-terminus. Modific...

journal_title：Molecular microbiology

authors： Mackay DT,Botting CH,Taylor GL,White MF

更新日期：2007-06-01 00:00:00

abstract：:The 29 kDa protein of pathogenic Entamoeba histolytica is a cysteine-rich surface antigen which we recently characterized by cDNA sequencing and by using monoclonal antibodies which differentiated between pathogenic and non-pathogenic clinical isolates. To determine the structure and biochemical attributes of this pro...

journal_title：Molecular microbiology

authors： Flores BM,Batzer MA,Stein MA,Petersen C,Diedrich DL,Torian BE

更新日期：1993-03-01 00:00:00

abstract：:The porins of Gram-negative bacteria are responsible for the 'molecular sieve' properties of the outer membrane. They form large water-filled channels which allow the diffusion of hydrophilic molecules into the periplasmic space. Owing to the strong hydrophilicity of their amino acid sequence and the nature of their s...

journal_title：Molecular microbiology

authors： Jeanteur D,Lakey JH,Pattus F

更新日期：1991-09-01 00:00:00

abstract：:Lipopolysaccharides (LPS), particularly the O-antigen component, are one of many virulence determinants necessary for Shigella flexneri pathogenesis. O-antigen biosynthesis is determined mostly by genes located in the rfb region of the chromosome. The rfc/wzy gene encodes the O-antigen polymerase, an integral membrane...

journal_title：Molecular microbiology

authors： Daniels C,Vindurampulle C,Morona R

更新日期：1998-06-01 00:00:00

abstract：:The analysis of completed archaeal genome sequences led to the identification of a set of approximately 10-20 genes whose protein products were inferred to be involved in chromosomal DNA replication. Until recently, however, little was known of the biochemical properties of these proteins. Here, I review recent progre...

journal_title：Molecular microbiology

authors： MacNeill SA

更新日期：2001-05-01 00:00:00

abstract：:Many physiological adjustments to nutrient changes involve ppGpp. Recent attempts to deduce ppGpp regulatory effects using proteomics or gene profiling can rigorously identify proteins or transcripts, but the functional significance is often unclear. Using a random screen for synthetic lethals we found a ppGpp-depende...

journal_title：Molecular microbiology

authors： Harinarayanan R,Murphy H,Cashel M

更新日期：2008-08-01 00:00:00

abstract：:Nitrogen metabolism genes of Bacillus subtilis are regulated by the availability of rapidly metabolizable nitrogen sources, but not by any mechanism analogous to the two-component Ntr regulatory system found in enteric bacteria. Instead, at least three regulatory proteins independently control the expression of gene p...

journal_title：Molecular microbiology

authors： Fisher SH

更新日期：1999-04-01 00:00:00

abstract：:Plasmodium parasites possess a single pyruvate dehydrogenase (PDH) enzyme complex that is localized to the plastid-like organelle known as the apicoplast. Unlike most eukaryotes, Plasmodium parasites lack a mitochondrial PDH. The PDH complex catalyses the conversion of pyruvate to acetyl-CoA, an important precursor fo...

journal_title：Molecular microbiology

authors： Pei Y,Tarun AS,Vaughan AM,Herman RW,Soliman JM,Erickson-Wayman A,Kappe SH

更新日期：2010-02-01 00:00:00

abstract：:Bacteria tightly regulate intracellular zinc levels to ensure sufficient zinc to support essential functions, while preventing toxicity. The bacterial response to zinc limitation includes the expression of putative zinc metallochaperones belonging to subfamily 1 of the COG0523 family of G3E GTPases. However, the clien...

journal_title：Molecular microbiology

authors： Chandrangsu P,Huang X,Gaballa A,Helmann JD

更新日期：2019-09-01 00:00:00

abstract：:Cpmk2, encoding a mitogen-activated protein (MAP) kinase from the ascomycete Claviceps purpurea, is an orthologue of SLT2 from Saccharomyces cerevisiae, the first isolated from a biotrophic, non-appressorium-forming pathogen. Deletion mutants obtained by a gene replacement approach show impaired vegetative properties ...

journal_title：Molecular microbiology

authors： Mey G,Held K,Scheffer J,Tenberge KB,Tudzynski P

更新日期：2002-10-01 00:00:00

abstract：:The most abundant oxidants controlling bacterial colonization on mucosal barrier epithelia are hypochlorous acid (HOCl), hypobromous acid (HOBr) and hypothiocyanous acid (HOSCN). All three oxidants are highly antimicrobial but little is known about their relative efficacies, their respective cellular targets, or what ...

journal_title：Molecular microbiology

authors： Groitl B,Dahl JU,Schroeder JW,Jakob U

更新日期：2017-11-01 00:00:00

abstract：:Misfolded proteins are usually arrested in the endoplasmic reticulum (ER) and degraded by the ER-associated degradation (ERAD) machinery. Several mutant alleles of PMA1, the gene coding for the plasma membrane H(+)-ATPase, render misfolded proteins that are retained in the ER and degraded by ERAD. A subset of misfolde...

journal_title：Molecular microbiology

authors： Mazón MJ,Eraso P,Portillo F

更新日期：2007-02-01 00:00:00

abstract：:The Frz pathway of Myxococcus xanthus controls cell reversal frequency to support directional motility during swarming and fruiting body formation. Previously, we showed that phosphorylation of the response regulator FrzZ correlates with reversal frequencies, suggesting that this activity represents the output of the ...

journal_title：Molecular microbiology

authors： Kaimer C,Zusman DR

更新日期：2016-04-01 00:00:00

abstract：:Translating ribosomes require elongation factor P (EF-P) to incorporate consecutive prolines (XPPX) into nascent peptide chains. The proteome of Corynebacterium glutamicum ATCC 13032 contains a total of 1,468 XPPX motifs, many of which are found in proteins involved in primary and secondary metabolism. We show here th...

journal_title：Molecular microbiology

authors： Pinheiro B,Petrov DP,Guo L,Martins GB,Bramkamp M,Jung K

更新日期：2020-10-04 00:00:00

abstract：:Overexpression of the MDR1 gene, encoding a multi-drug efflux pump of the major facilitator superfamily, is a major cause of resistance to the widely used antifungal agent fluconazole and other toxic substances in the fungal pathogen Candida albicans. We found that all tested clinical and in vitro generated C. albican...

journal_title：Molecular microbiology

authors： Dunkel N,Blass J,Rogers PD,Morschhäuser J

更新日期：2008-08-01 00:00:00

abstract：:Bacterial protein secretion is catalysed by the SecYEG protein-conducting channel complexed with the SecA ATPase motor. To gain insight into the SecA-SecYEG interaction we used peptide arrays, thermodynamic quantification, mutagenesis and functional assays. Our data reveal that: (i) SecA binds with low affinity on sev...

journal_title：Molecular microbiology

authors： Karamanou S,Bariami V,Papanikou E,Kalodimos CG,Economou A

更新日期：2008-10-01 00:00:00

abstract：:The HypB protein from Bradyrhizobium japonicum is a metal-binding GTPase required for hydrogenase expression. In-frame mutagenesis of hypB resulted in strains that were partially or completely deficient in hydrogenase expression, depending on the degree of disruption of the gene. Complete deletion of the gene yielded ...

journal_title：Molecular microbiology

authors： Olson JW,Fu C,Maier RJ

更新日期：1997-04-01 00:00:00

abstract：:In conditions of halted or limited genome replication, like those experienced in sporulating cells of Bacillus subtilis, a more immediate detriment caused by DNA damage is altering the transcriptional programme that drives this developmental process. Here, we report that mfd, which encodes a conserved bacterial protei...

journal_title：Molecular microbiology

authors： Ramírez-Guadiana FH,Del Carmen Barajas-Ornelas R,Ayala-García VM,Yasbin RE,Robleto E,Pedraza-Reyes M

更新日期：2013-12-01 00:00:00

abstract：:We used in vivo oxidative cross-linking of engineered cysteine pairs to assess conformational changes in the four-helix transmembrane domain of chemoreceptor Trg. Extending previous work, we searched for and found a fourth cross-linking pair that spanned the intrasubunit interface between transmembrane helix 1 (TM1) a...

journal_title：Molecular microbiology

authors： Beel BD,Hazelbauer GL

更新日期：2001-05-01 00:00:00

abstract：:Until recently, rare-earth elements (REEs) had been thought to be biologically inactive. This view changed with the discovery of the methanol dehydrogenase XoxF that strictly relies on REEs for its activity. Some methylotrophs only contain xoxF, while others, including the model phyllosphere colonizer Methylobacterium...

journal_title：Molecular microbiology

authors： Ochsner AM,Hemmerle L,Vonderach T,Nüssli R,Bortfeld-Miller M,Hattendorf B,Vorholt JA

更新日期：2019-05-01 00:00:00

abstract：:Each bacterium has to co-ordinate its growth with division to ensure genetic stability of the population. Consequently, cell division and growth are tightly regulated phenomena, albeit different bacteria utilise one of several alternative regulatory mechanisms to maintain control. Here we consider GpsB, which is linke...

journal_title：Molecular microbiology

authors： Rismondo J,Cleverley RM,Lane HV,Großhennig S,Steglich A,Möller L,Mannala GK,Hain T,Lewis RJ,Halbedel S

更新日期：2016-03-01 00:00:00

abstract：:The availability of nutrients is a major determinant for the timing of morphogenesis and antibiotic production in the soil-dwelling bacterium Streptomyces coelicolor. Here we show that N-acetylglucosamine transport, the first step of an important nutrient signalling cascade, is mediated by the NagE2 permease of the ph...

journal_title：Molecular microbiology

authors： Nothaft H,Rigali S,Boomsma B,Swiatek M,McDowall KJ,van Wezel GP,Titgemeyer F

更新日期：2010-03-01 00:00:00

abstract：:The structures of the choline-dependent pneumococcal murein hydrolases, LYTA amidase and CPL1 lysozyme, and the choline-independent CPL7 lysozyme were analysed by controlled proteolytic digestions. The trypsin cleavage of the CPL1 and CPL7 lysozymes produced two resistant polypeptides, F1 and F7 respectively, correspo...

journal_title：Molecular microbiology

authors： Sanz JM,Díaz E,García JL

更新日期：1992-04-01 00:00:00

abstract：:The identical partial deep-core structure of Hep alpha 1-3Hep alpha 1-5KDO in Salmonella typhimurium LT2 LPS and Neisseria gonorrhoeae LOS enabled us to isolate a DNA fragment from N. gonorrhoeae that was able to complement the alpha 1,5 LOS heptosyltransferase defect in the S. typhimurium rfaC630 (SA1377) mutant. SDS...

journal_title：Molecular microbiology

authors： Zhou D,Lee NG,Apicella MA

更新日期：1994-11-01 00:00:00