Abstract：
:The Vipp1 protein is essential in cyanobacteria and chloroplasts for the maintenance of photosynthetic function and thylakoid membrane architecture. To investigate its mode of action we generated strains of the cyanobacteria Synechocystis sp. PCC6803 and Synechococcus sp. PCC7942 in which Vipp1 was tagged with green fluorescent protein at the C-terminus and expressed from the native chromosomal locus. There was little perturbation of function. Live-cell fluorescence imaging shows dramatic relocalisation of Vipp1 under high light. Under low light, Vipp1 is predominantly dispersed in the cytoplasm with occasional concentrations at the outer periphery of the thylakoid membranes. High light induces Vipp1 coalescence into localised puncta within minutes, with net relocation of Vipp1 to the vicinity of the cytoplasmic membrane and the thylakoid membranes. Pull-downs and mass spectrometry identify an extensive collection of proteins that are directly or indirectly associated with Vipp1 only after high-light exposure. These include not only photosynthetic and stress-related proteins but also RNA-processing, translation and protein assembly factors. This suggests that the Vipp1 puncta could be involved in protein assembly. One possibility is that Vipp1 is involved in the formation of stress-induced localised protein assembly centres, enabling enhanced protein synthesis and delivery to membranes under stress conditions.
journal_name
Mol Microbioljournal_title
Molecular microbiologyauthors
Bryan SJ,Burroughs NJ,Shevela D,Yu J,Rupprecht E,Liu LN,Mastroianni G,Xue Q,Llorente-Garcia I,Leake MC,Eichacker LA,Schneider D,Nixon PJ,Mullineaux CWdoi
10.1111/mmi.12826subject
Has Abstractpub_date
2014-10-13 00:00:00eissn
0950-382Xissn
1365-2958pub_type
杂志文章abstract：:Visual inspection showed clear evidence of a history of intraspecies recombinational exchanges within the neighbouring meningococcal shikimate dehydrogenase (aroE) and glutamine synthetase (glnA) genes, which was supported by the non-congruence of the trees constructed from the sequences of these genes from different ...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2958.1997.2681633.x
更新日期：1997-02-01 00:00:00
abstract：:A number of strains of the widespread aerobic soil bacterium, Bacillus sphaericus, possess crystalline inclusions of a toxin lethal to a variety of insect (larvae) which are vectors of major tropical diseases. Partial amino acid sequence data from one strain, B. sphaericus 2362 have permitted us to design oligonucleot...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.1987.tb00511.x
更新日期：1987-09-01 00:00:00
abstract：:The recombination of short homologous ends in Escherichia coli has been known for 30 years, and it is often used for both site-directed mutagenesis and in vivo cloning. For cloning, a plasmid and target DNA fragments were converted into linear DNA fragments with short homologous ends, which are joined via recombinatio...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/mmi.14677
更新日期：2020-12-29 00:00:00
abstract：:During pathogenesis, Mycobacterium tuberculosis (Mtb) colonizes environments, such as the macrophage or necrotic granuloma, that are acidic and rich in cholesterol and fatty acids. The goal of this study was to examine how acidic pH and available carbon sources interact to regulate Mtb physiology. Here we report that ...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/mmi.12688
更新日期：2014-10-01 00:00:00
abstract：:The presence of capsular exopolysaccharide (EPS) in Mollicutes has been inferred from electron micrographs for over 50 years without conclusive data to support the production of complex carbohydrates by the organism. Mycoplasma pulmonis binds the lectin Griffonia simplicifolia I (GS-I), which is specific for terminal ...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.2009.06720.x
更新日期：2009-06-01 00:00:00
abstract：:Meningococcal gdhA, encoding the NADP-specific l-glutamate dehydrogenase (NADP-GDH), is essential for systemic infection in an infant rat model. In this paper, a limited transcriptional analysis detected differences in gdhA expression among clinical isolates. In strains expressing high levels of gdhA mRNA, two promote...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.2003.03947.x
更新日期：2004-03-01 00:00:00
abstract：:In the yeast Saccharomyces cerevisiae, genes involved in phospholipid biosynthesis are activated by ICRE (inositol/choline-responsive element) up-stream motifs and the corresponding heterodimeric binding factor, Ino2 + Ino4. Both Ino2 and Ino4 contain basic helix-loop-helix (bHLH) domains required for ICRE binding, wh...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2958.2003.03501.x
更新日期：2003-05-01 00:00:00
abstract：:Billions of years of evolution have yielded today's complex metabolic networks driven by efficient and highly specialized enzymes. In contrast, the metabolism of the earliest cellular life forms was likely much simpler with only a few enzymes of comparatively low activity. It has been speculated that these early enzym...
journal_title：Molecular microbiology
pub_type： 社论
doi：10.1111/mmi.13742
更新日期：2017-08-01 00:00:00
abstract：:We screened the putative rap-phr (response regulator aspartyl-phosphate phosphatase-phosphatase regulator) systems identified in the Bacillus subtilis genome for a rap gene that affects aprE (alkaline protease gene) expression by using a multicopy plasmid. We found that rapG was involved in the regulation of aprE, whi...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2958.2003.03665.x
更新日期：2003-09-01 00:00:00
abstract：:The sex pheromone cAM373 of Enterococcus faecalis and the related staph-cAM373 of Staphylococcus aureus were found to correspond to heptapeptides located within the C-termini of the signal sequences of putative prelipoproteins. The deduced mature forms of the lipoproteins share no detectable homology and presumably se...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2958.2002.02922.x
更新日期：2002-05-01 00:00:00
abstract：:Protein phosphatases 2C are a family of conserved enzymes involved in many aspects of the cell biology. We reported that, in the yeast Saccharomyces cerevisiae, overexpression of the Ptc3p isoform resulted in increased lithium tolerance in the hypersensitive hal3 background. We have found that the tolerance induced by...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.2006.05370.x
更新日期：2006-10-01 00:00:00
abstract：:The fungal pathogen Candida albicans can transition from budding to hyphal growth, which promotes biofilm formation and invasive growth into tissues. Stimulation of adenylyl cyclase to form cAMP induces hyphal morphogenesis. The failure of cells lacking adenylyl cyclase (cyr1Δ) to form hyphae has suggested that cAMP s...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/mmi.13588
更新日期：2017-03-01 00:00:00
abstract：:Elevated expression of the plasma membrane drug efflux pump proteins Cdr1p and Cdr2p was shown to accompany decreased azole susceptibility in Candida albicans clinical isolates. DNA sequence analysis revealed extensive allelic heterozygosity, particularly of CDR2. Cdr2p alleles showed different abilities to transport ...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.2006.05357.x
更新日期：2006-10-01 00:00:00
abstract：:Ustilago maydis, the causal agent of corn smut disease, displays dimorphic growth in which it alternates between a budding haploid saprophyte and a filamentous dikaryotic pathogen. We are interested in identifying the genetic determinants of filamentous growth and pathogenicity in U. maydis. To do this, we have taken ...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2958.1999.01610.x
更新日期：1999-11-01 00:00:00
abstract：:Ribonucleases J1 and J2 of Bacillus subtilis are evolutionarily conserved enzymes combining an endoribonucleolytic and a 5'-3' exoribonucleolytic activity in a single polypeptide. Their endoribonucleolytic cleavage specificity resembles that of RNase E, a key player in the processing and degradation of RNA in Escheric...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.2008.06400.x
更新日期：2008-10-01 00:00:00
abstract：:The csiD-ygaF-gabDTP region in the Escherichia coli genome represents a cluster of sigma S-controlled genes. Here, we investigated promoter structures, sigma factor dependencies, potential co-regulation and environmental regulatory patterns for all of these genes. We find that this region constitutes a complex operon ...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2958.2003.03867.x
更新日期：2004-02-01 00:00:00
abstract：:While examining the molecular basis for the lack of actin-based motility for the non-pathogenic spotted fever group (SFG) R. peacockii, we identified a novel insertion sequence (IS) element, ISRpe1, disrupting the coding sequence of rickA, demonstrated to induce actin-tail polymerization for the SFG rickettsiae. This ...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.2005.04806.x
更新日期：2005-10-01 00:00:00
abstract：:Proteic toxin-antitoxin (TA) loci were first identified in bacterial plasmids, and they were regarded as involved in stable plasmid maintenance by a so-called 'addiction' mechanism. Later, chromosomally encoded TA loci were identified and their function ascribed to survival mechanisms when bacteria were subjected to s...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.2006.05027.x
更新日期：2006-02-01 00:00:00
abstract：:The partition system of the P1 plasmid, P1par, consists of the ParA and ParB proteins and a cis-acting site, parS. It is responsible for the orderly segregation of plasmid copies to daughter cells. Plasmids with null mutations in parA or parB replicate normally, but missegregate. ParB binds specifically to the parS si...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2958.1997.4761842.x
更新日期：1997-09-01 00:00:00
abstract：:Chromalveolates like the diatom Phaeodactylum tricornutum arose through the uptake of a red alga by a phagotrophic protist, a process termed secondary endosymbiosis. In consequence, the plastids are surrounded by two additional membranes compared with primary plastids. This plastid morphology poses additional barriers...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.2010.07142.x
更新日期：2010-05-01 00:00:00
abstract：:The cloned Citrobacter freundii ampC beta-lactamase is inducible in the presence of its regulatory gene ampR in Escherichia coli (Lindberg et al., 1985). The basal level of expression and inducibility are affected by two E. coli proteins encoded by the closely linked ampD and ampE genes. Deletion of both genes led to ...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.1989.tb00259.x
更新日期：1989-08-01 00:00:00
abstract：:A 2.6 kb ClaI-BamHI DNA fragment of megaplasmid 2 of Rhizobium meliloti 2011 was found to carry genes involved in exopolysaccharide synthesis and infection of alfalfa nodules. The analysis of the nucleotide sequence of this DNA fragment revealed the existence of two open reading frames (ORFs) running in opposite direc...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.1991.tb00799.x
更新日期：1991-06-01 00:00:00
abstract：:We describe the application of immunofluorescence microscopy to visualization of the subcellular localization of proteins involved in coat morphogenesis and chromosome packaging during the process of sporulation in Bacillus subtilis. In confirmation and extension of previous findings, we show that SpolVA, which is res...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.1995.mmi_18030459.x
更新日期：1995-11-01 00:00:00
abstract：:The export of large negatively charged capsular polysaccharides across the outer membrane represents a significant challenge to Gram negative bacteria. In the case of Escherichia coli group 2 capsular polysaccharides, the mechanism of export across the outer membrane was unknown, with no identified candidate outer mem...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.2005.05010.x
更新日期：2006-02-01 00:00:00
abstract：:Most of the genes required for the conjugative transfer of DNA are encoded by the 33 kb transfer (tra) operon of F-like conjugative plasmids. Transcription of the tra operon is positively regulated by the TraJ transcriptional activator which, in turn, is negatively regulated by the FinOP fertility inhibition system. T...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.1994.tb02177.x
更新日期：1994-11-01 00:00:00
abstract：:The volume of electron flow through the cbb3 branch of the electron transport chain and the redox state of the quinone pool generate signals that regulate photosynthesis gene expression in Rhodobacter sphaeroides. An inhibitory signal is generated at the level of the catalytic subunit of the cbb3 cytochrome c oxidase ...
journal_title：Molecular microbiology
pub_type： 杂志文章,评审
doi：10.1111/j.1365-2958.2001.02299.x
更新日期：2001-03-01 00:00:00
abstract：:Members of the bacterial genus Brucella are facultative intracellular pathogens that reside predominantly within membrane-bound compartments within two host cell types, macrophages and placental trophoblasts. Within macrophages, the brucellae route themselves to an intracellular compartment that is favourable for surv...
journal_title：Molecular microbiology
pub_type： 杂志文章,评审
doi：10.1111/j.1365-2958.2004.04017.x
更新日期：2004-05-01 00:00:00
abstract：:Whereas in Bacillus subtilis, a general stress response stimulon under the control of a single sigma factor (SigB) is induced by different physiological and environmental stresses (heat, salt or ethanol shock), in Streptomyces coelicolor, these environmental stresses induce independent sets of proteins, and its genome...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2958.2003.03302.x
更新日期：2003-02-01 00:00:00
abstract：:Atovaquone represents a class of antimicrobial agents with a broad-spectrum activity against various parasitic infections, including malaria, toxoplasmosis and Pneumocystis pneumonia. In malaria parasites, atovaquone inhibits mitochondrial electron transport at the level of the cytochrome bc1 complex and collapses mit...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2958.1999.01515.x
更新日期：1999-08-01 00:00:00
abstract：:Strains of Escherichia coli have been produced which express very high levels of the tRNA(1Leu) isoacceptor. This was accomplished by transforming cells with plasmids containing the leuV operon which encodes three copies of the tRNA(1Leu) gene. Most transformants grew very slowly and exhibited a 15-fold increase in ce...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.1993.tb01116.x
更新日期：1993-01-01 00:00:00
