Multifunctional enzymes from reduced genomes - model proteins for simple primordial metabolism?

abstract：:The Staphylococcus epidermidis genes icaABC are involved in the synthesis of the polysaccharide intercellular adhesin (PIA), which is located mainly on the cell surface, as shown by immunofluorescence studies with PIA-specific antiserum. PIA was shown to be a linear beta-1,6-linked glucosaminoglycan composed of at lea...

journal_title：Molecular microbiology

authors： Heilmann C,Schweitzer O,Gerke C,Vanittanakom N,Mack D,Götz F

更新日期：1996-06-01 00:00:00

abstract：:Natural transformation is a potent driver for genetic diversification in bacterial populations. It involves exogenous DNA binding, uptake, transport and internalization into the cytoplasm, where DNA can be processed and integrated into the host chromosome. Direct visualisation of transforming DNA (tDNA) has been limit...

journal_title：Molecular microbiology

authors： Corbinais C,Mathieu A,Kortulewski T,Radicella JP,Marsin S

更新日期：2016-09-01 00:00:00

abstract：:In May 2004, over 100 bacteriologists from 19 different countries discussed recent progress in identification and understanding of individual signal transfer mechanisms in bacteria and in the mutual interactions between these systems to form a functional living cell. The meeting was held in San Feliu and supported by ...

journal_title：Molecular microbiology

authors： Hellingwerf KJ

更新日期：2004-10-01 00:00:00

abstract：:The septum-located DNA translocase, FtsK, acts to co-ordinate the late steps of Escherichia coli chromosome segregation with cell division. The FtsK gamma regulatory subdomain interacts with 8 bp KOPS DNA sequences, which are oriented from the replication origin to the terminus region (ter) in each arm of the chromoso...

journal_title：Molecular microbiology

authors： Sivanathan V,Emerson JE,Pages C,Cornet F,Sherratt DJ,Arciszewska LK

更新日期：2009-02-01 00:00:00

abstract：:Most of the structural components of the flagellum of Salmonella typhimurium are exported through a flagellum-specific pathway, which is a member of the family of type III secretory pathways. The export apparatus for this process is poorly understood. A previous study has shown that two proteins, about 23 and 26 kDa i...

journal_title：Molecular microbiology

authors： Fan F,Ohnishi K,Francis NR,Macnab RM

更新日期：1997-12-01 00:00:00

abstract：:Sphingomyelin is the main sphingolipid in Trypanosoma brucei, the causative agent of African sleeping sickness. In vitro and in vivo characterization of the T. brucei neutral sphingomyelinase demonstrates that it is directly involved in sphingomyelin catabolism. Gene knockout studies in the bloodstream form of the par...

journal_title：Molecular microbiology

authors： Young SA,Smith TK

更新日期：2010-06-01 00:00:00

abstract：:We have demonstrated hypervariability of native short-motif repeats (microsatellites) in Escherichia coli. Twenty-five of the longest microsatellites in the E. coli genome were identified. These were analysed for length variability among 22 wild-type (non-mutator) isolates from the E. coli collection of reference (ECO...

journal_title：Molecular microbiology

authors： Metzgar D,Thomas E,Davis C,Field D,Wills C

更新日期：2001-01-01 00:00:00

abstract：:Avermectins produced by Streptomyces avermitilis are effective anthelmintic agents. The autoregulatory signalling molecule that triggers avermectin biosynthesis is a novel butenolide-type molecule, avenolide, rather than common γ-butyrolactones (GBLs). We identified AvaR2, a pseudo GBL receptor homologue, as an import...

journal_title：Molecular microbiology

authors： Zhu J,Sun D,Liu W,Chen Z,Li J,Wen Y

更新日期：2016-11-01 00:00:00

abstract：:The Gram-negative bacterium Vibrio cholerae is the infectious agent responsible for the disease Asiatic cholera. The genes required for V. cholerae virulence, such as those encoding the cholera toxin (CT) and toxin-coregulated pilus (TCP), are controlled by a cascade of transcriptional activators. Ultimately, the dire...

journal_title：Molecular microbiology

authors： Withey JH,DiRita VJ

更新日期：2005-05-01 00:00:00

abstract：:Infections with the azole-refractory yeast Candida glabrata are now commonly treated with the echinocandins caspofungin (CSF) or micafungin (MCF). True resistance (>  32-fold decreased susceptibility) to these lipopeptide inhibitors of cell wall synthesis is rare and strictly associated with mutations in integral memb...

journal_title：Molecular microbiology

authors： Healey KR,Katiyar SK,Raj S,Edlind TD

更新日期：2012-10-01 00:00:00

abstract：:The largest subunit of RNA polymerase II, Rpb1, contains an unusual C-terminal domain (CTD) composed of numerous repeats of the YSPTSPS consensus sequence. This sequence is the target of post-translational modifications such as phosphorylation, glycosylation, methylation and transitions between stereoisomeric states, ...

journal_title：Molecular microbiology

authors： Drogat J,Hermand D

更新日期：2012-06-01 00:00:00

abstract：:In Saccharomyces cerevisiae, glucose activation of cAMP synthesis requires both the presence of the G-protein-coupled receptor (GPCR) system, Gpr1-Gpa2, and uptake and phosphorylation of the sugar. In a hxt-null strain that lacks all physiologically important glucose carriers, glucose transport as well as glucose-indu...

journal_title：Molecular microbiology

authors： Rolland F,De Winde JH,Lemaire K,Boles E,Thevelein JM,Winderickx J

更新日期：2000-10-01 00:00:00

abstract：:Chlamydiae are obligate intracellular bacteria which undergo a unique developmental cycle, alternating between non-replicative elementary bodies (EBs) and replicative reticulate bodies (RBs). The transition from RB to EB is characterized by condensation of the chromosome into a dense nucleoid structure. The chlamydial...

journal_title：Molecular microbiology

authors： Barry CE 3rd,Brickman TJ,Hackstadt T

更新日期：1993-07-01 00:00:00

abstract：:Ail, a multifunctional outer membrane protein of Yersinia pestis, confers cell binding, Yop delivery and serum resistance activities. Resistance to complement proteins in serum is critical for the survival of Y. pestis during the septicemic stage of plague infections. Bacteria employ a variety of tactics to evade the ...

journal_title：Molecular microbiology

authors： Thomson JJ,Plecha SC,Krukonis ES

更新日期：2019-01-01 00:00:00

abstract：:Previous studies have shown that in Saccharomyces cerevisiae the mitochondrial and cytoplasmic forms of alanyl-tRNA synthetase are encoded by a single nuclear gene, ALA1, through alternative use of in-frame successive ACG triplets and a downstream AUG triplet. Here we show that despite the obvious participation of the...

journal_title：Molecular microbiology

authors： Huang HY,Tang HL,Chao HY,Yeh LS,Wang CC

更新日期：2006-04-01 00:00:00

abstract：:Ribonucleases J1 and J2 of Bacillus subtilis are evolutionarily conserved enzymes combining an endoribonucleolytic and a 5'-3' exoribonucleolytic activity in a single polypeptide. Their endoribonucleolytic cleavage specificity resembles that of RNase E, a key player in the processing and degradation of RNA in Escheric...

journal_title：Molecular microbiology

authors： Mäder U,Zig L,Kretschmer J,Homuth G,Putzer H

更新日期：2008-10-01 00:00:00

abstract：:In response to nitrate availability, Escherichia coli regulates the synthesis of a number of enzymes involved in anaerobic respiration and fermentation. When nitrate is present, nitrate reductase (narGHJI) gene expression is induced, while expression of the DMSO/TMAO reductase (dmsABC), fumarate reductase (frdABCD) an...

journal_title：Molecular microbiology

authors： Chiang RC,Cavicchioli R,Gunsalus RP

更新日期：1992-07-01 00:00:00

abstract：:Monoclonal antibodies against the lipopolysaccharide (LPS) of the deep rough mutant I-69 Rd-/b+ of Haemophilus influenzae were obtained after immunization of mice with sheep erythrocytes which had been coated with de-O-acylated LPS. Characterization of antibodies was performed by enzyme immuno assay (EIA) using LPS or...

journal_title：Molecular microbiology

authors： Rozalski A,Brade L,Kosma P,Moxon R,Kusumoto S,Brade H

更新日期：1997-02-01 00:00:00

abstract：:In response to discrete environmental cues, Saccharomyces cerevisiae cells adjust patterns of gene expression and protein activity to optimize metabolism. Nutrient-sensing systems situated in the plasma membrane (PM) of yeast have only recently been discovered. Ssy1p is one of three identified components of the Ssy1p-...

journal_title：Molecular microbiology

authors： Forsberg H,Gilstring CF,Zargari A,Martínez P,Ljungdahl PO

更新日期：2001-10-01 00:00:00

abstract：:Pathogenic enterotoxigenic Escherichia coli (ETEC) are the major bacterial cause of diarrhea in young children in developing countries and in travelers, causing significant mortality in children. Adhesive fimbriae are a prime virulence factor for ETEC, initiating colonization of the small intestinal epithelium. Simila...

journal_title：Molecular microbiology

authors： Mortezaei N,Epler CR,Shao PP,Shirdel M,Singh B,McVeigh A,Uhlin BE,Savarino SJ,Andersson M,Bullitt E

更新日期：2015-01-01 00:00:00

abstract：:In Escherichia coli, after DNA damage, the SOS response increases the transcription (and protein levels) of approximately 50 genes. As DNA repair ensues, the level of transcription returns to homeostatic levels. ClpXP and other proteases return the high levels of several SOS proteins to homeostasis. When all SOS genes...

journal_title：Molecular microbiology

authors： Warr AR,Klimova AN,Nwaobasi AN,Sandler SJ

更新日期：2019-02-01 00:00:00

abstract：:The cell walls of Sulfolobales species consist of proteinaceous S-layers assembled from two polypeptides, SlaA and SlaB. We isolated the large S-layer protein of Acidianus ambivalens and both S-layer subunits of Sulfolobus solfataricus and Metallosphaera sedula, respectively. The slaAB genes, lying adjacently in the c...

journal_title：Molecular microbiology

authors： Veith A,Klingl A,Zolghadr B,Lauber K,Mentele R,Lottspeich F,Rachel R,Albers SV,Kletzin A

更新日期：2009-07-01 00:00:00

abstract：:Interpretation of the constantly expanding body of genomic information requires that the function of each gene be established. Here we report the genomic analysis and structural modelling of a previously uncharacterized redox-metabolism protein UrdA (SO_4620) of Shewanella oneidensis MR-1, which led to a discovery of ...

journal_title：Molecular microbiology

authors： Bogachev AV,Bertsova YV,Bloch DA,Verkhovsky MI

更新日期：2012-12-01 00:00:00

abstract：:The mechanistic details of the pathogenesis of Chlamydia, an obligate intracellular pathogen of global importance, have eluded scientists due to the scarcity of traditional molecular genetic tools to investigate this organism. Here we report a chemical biology strategy that has uncovered the first essential protease f...

journal_title：Molecular microbiology

authors： Gloeckl S,Ong VA,Patel P,Tyndall JD,Timms P,Beagley KW,Allan JA,Armitage CW,Turnbull L,Whitchurch CB,Merdanovic M,Ehrmann M,Powers JC,Oleksyszyn J,Verdoes M,Bogyo M,Huston WM

更新日期：2013-08-01 00:00:00

abstract：:Listeria monocytogenes FrvA (Lmo0641) is critical for virulence in the mouse model and is an ortholog of the Bacillus subtilis Fur- and PerR-regulated Fe(II) efflux P1B4 -type ATPase PfeT. Previously, FrvA was suggested to protect against heme toxicity. Here, we demonstrate that an frvA mutant is sensitive to iron int...

journal_title：Molecular microbiology

authors： Pi H,Patel SJ,Argüello JM,Helmann JD

更新日期：2016-06-01 00:00:00

abstract：:Transcriptional regulation is the key to ensuring that proteins are expressed at the proper time and the proper amount. In Escherichia coli, the transcription factor cAMP receptor protein (CRP) is responsible for much of this regulation. Questions remain, however, regarding the regulation of CRP activity itself. Here,...

journal_title：Molecular microbiology

authors： Davis R,Écija-Conesa A,Gallego-Jara J,de Diego T,Filippova EV,Kuffel G,Anderson WF,Gibson BW,Schilling B,Canovas M,Wolfe AJ

更新日期：2018-01-01 00:00:00

abstract：:Trypanothione reductase (TR), a flavoprotein oxidoreductase central to the unique thiol-redox system that operates in trypanosomatid protozoa, has been proposed as a potential target for the chemotherapy of trypanosomatid infections. In this study, targeted gene replacement was used to obtain evidence that TR is an es...

journal_title：Molecular microbiology

authors： Tovar J,Wilkinson S,Mottram JC,Fairlamb AH

更新日期：1998-07-01 00:00:00

abstract：:Many bacterial gene regulatory circuits are controlled by temperature. Temperature-mediated regulation occurs at the level of transcription and translation. Supercoiling, changes in mRNA conformation and protein conformation are all implicated in thermosensing. Bacterial virulence functions are often temperature regul...

journal_title：Molecular microbiology

authors： Hurme R,Rhen M

更新日期：1998-10-01 00:00:00

abstract：:In Escherichia coli the flagellar regulon consists of more than 60 genes organized in three hierarchically and temporally regulated transcriptional classes. The flagellar sigma factor FliA (sigma(28)) is responsible for class 3 expression and, in the early phase of flagellar assembly, is inhibited by its anti-sigma fa...

journal_title：Molecular microbiology

authors： Barembruch C,Hengge R

更新日期：2007-07-01 00:00:00

abstract：:Cell cycle transitions are often triggered by the proteolysis of key regulatory proteins. In Caulobacter crescentus, the G1-S transition involves the degradation of an essential DNA-binding response regulator, CtrA, by the ClpXP protease. Here, we show that another critical cell cycle regulator, SciP, is also degraded...

journal_title：Molecular microbiology

authors： Gora KG,Cantin A,Wohlever M,Joshi KK,Perchuk BS,Chien P,Laub MT

更新日期：2013-03-01 00:00:00