An unusual pattern of protein expression and localization of yeast alanyl-tRNA synthetase isoforms.

abstract：:The Clostridium difficile toxA and toxB genes, encoding cytotoxic and enterotoxic proteins responsible for antibiotic-associated colitis and pseudomembranous colitis, were shown to be transcribed both from gene-specific promoters and from promoters of upstream genes. However, the gene-specific transcripts represented ...

journal_title：Molecular microbiology

authors： Dupuy B,Sonenshein AL

更新日期：1998-01-01 00:00:00

abstract：:The presence of capsular exopolysaccharide (EPS) in Mollicutes has been inferred from electron micrographs for over 50 years without conclusive data to support the production of complex carbohydrates by the organism. Mycoplasma pulmonis binds the lectin Griffonia simplicifolia I (GS-I), which is specific for terminal ...

journal_title：Molecular microbiology

authors： Daubenspeck JM,Bolland JR,Luo W,Simmons WL,Dybvig K

更新日期：2009-06-01 00:00:00

abstract：:The recent emergence of indolent and rapidly fatal drug-resistant strains of Mycobacterium tuberculosis has renewed interest in defining the molecular mechanisms of drug resistance in the tubercle bacilli. In this report, we have examined the mechanism of resistance to streptomycin (Sm) in M. tuberculosis through the ...

journal_title：Molecular microbiology

authors： Nair J,Rouse DA,Bai GH,Morris SL

更新日期：1993-11-01 00:00:00

abstract：:Ustilago maydis, the causal agent of corn smut disease, displays dimorphic growth in which it alternates between a budding haploid saprophyte and a filamentous dikaryotic pathogen. We are interested in identifying the genetic determinants of filamentous growth and pathogenicity in U. maydis. To do this, we have taken ...

journal_title：Molecular microbiology

authors： Mayorga ME,Gold SE

更新日期：1999-11-01 00:00:00

abstract：:The translational roles of the Shine-Dalgarno sequence, the initiation codon, the space between them, and the second codon have been studied. The Shine-Dalgarno sequence UAAGGAGG initiated translation roughly four times more efficiently than did the shorter AAGGA sequence. Each Shine-Dalgarno sequence required a minim...

journal_title：Molecular microbiology

authors： Ringquist S,Shinedling S,Barrick D,Green L,Binkley J,Stormo GD,Gold L

更新日期：1992-05-01 00:00:00

abstract：:Expression of the cysteine regulon in Salmonella typhimurium and Escherichia coli is controlled by the LysR-type transcriptional activator CysB and by the inducer N-acetyl-L-serine. Sulphide and thiosulphate are anti-inducers. Two highly purified constitutive CysB proteins, CysB(T149M) and CysB(T149P), were found to b...

journal_title：Molecular microbiology

authors： Colyer TE,Kredich NM

更新日期：1996-07-01 00:00:00

abstract：:Comparison of the Leishmania infantum genome with Leishmania braziliensis and Leishmania major genomes has identified 25 L. infantum species-specific genes that are absent or pseudogenes in L. major and L. braziliensis. To determine whether these L. infantum species-specific genes are involved in visceral Leishmania i...

journal_title：Molecular microbiology

authors： Zhang WW,Matlashewski G

更新日期：2010-07-01 00:00:00

abstract：:The alternative sigma factor sigma 54 is required for transcription of nitrogen fixation genes in Klebsiella pneumoniae and other diazotrophs. The nif genes, and other E sigma 54-dependent genes whose products are necessary for a wide range of processes, are postively regulated. A unifying model that is well supported...

journal_title：Molecular microbiology

authors： Buck M,Cannon W

更新日期：1992-06-01 00:00:00

abstract：:The Bacillus subtilis ssuBACD gene cluster is required for the utilization of aliphatic sulphonates as sulphur sources. The regulation of expression of the ssu genes was studied in constructs carrying chromosomal transcriptional fusions of the ssu promoter region to lacZ. When sulphate or cystine served as sulphur sou...

journal_title：Molecular microbiology

authors： van der Ploeg JR,Barone M,Leisinger T

更新日期：2001-03-01 00:00:00

abstract：:The gene encoding the multifunctional enzyme enniatin synthetase from Fusarium scirpi (esyn1) was isolated and characterized by transcriptional mapping and expression studies in Escherichia coli. This is the first example of a gene encoding an N-methyl peptide synthetase. The nucleotide sequence revealed an open readi...

journal_title：Molecular microbiology

authors： Haese A,Schubert M,Herrmann M,Zocher R

更新日期：1993-03-01 00:00:00

abstract：:The bvg locus contains two genes, bvgA and bvgS, which control the expression of the virulence-associated genes in Bordetella species by a system similar to the two-component systems used by a variety of bacterial species to respond to environmental stimuli. We determined the nucleotide sequence of the bvg loci of Bor...

journal_title：Molecular microbiology

authors： Aricò B,Scarlato V,Monack DM,Falkow S,Rappuoli R

更新日期：1991-10-01 00:00:00

abstract：:The replication-initiator protein DnaA is ubiquitous in the eubacterial world. It binds to an asymmetric 9 bp consensus DNA sequence, the DnaA box. Besides its primary function as an initiator, it acts as a transcription factor that represses or activates several genes, or terminates transcription, depending on the lo...

journal_title：Molecular microbiology

authors： Messer W,Weigel C

更新日期：1997-04-01 00:00:00

abstract：:Strains of Escherichia coli have been produced which express very high levels of the tRNA(1Leu) isoacceptor. This was accomplished by transforming cells with plasmids containing the leuV operon which encodes three copies of the tRNA(1Leu) gene. Most transformants grew very slowly and exhibited a 15-fold increase in ce...

journal_title：Molecular microbiology

authors： Wahab SZ,Rowley KO,Holmes WM

更新日期：1993-01-01 00:00:00

abstract：:Biofilm formation is a social behaviour that generates favourable conditions for sustained survival in the natural environment. For the Gram-positive bacterium Bacillus subtilis the process involves the differentiation of cell fate within an isogenic population and the production of communal goods that form the biofil...

journal_title：Molecular microbiology

authors： Cairns LS,Hobley L,Stanley-Wall NR

更新日期：2014-08-01 00:00:00

abstract：:Eukaryotic cells have evolved a complex set of intracellular organelles, each of which possesses a specific complement of enzymes and performs unique metabolic functions. This compartmentalization of cellular functions provides a level of metabolic control not available to prokaryotes. However, it presents the eukaryo...

journal_title：Molecular microbiology

authors： Aitchison JD,Nuttley WM,Szilard RK,Brade AM,Glover JR,Rachubinski RA

更新日期：1992-12-01 00:00:00

abstract：:Polycyclic aromatic hydrocarbons (PAHs) are persistent and toxic environmental pollutants that accumulate in anoxic habitats. With the exception of naphthalene, nothing is known about the microbial degradation of PAH in these environments. The challenge that must be met in anaerobic PAH degradation is the destabilizat...

journal_title：Molecular microbiology

authors： Sawers RG

更新日期：2015-01-01 00:00:00

abstract：:Integrative and conjugative elements (ICEs), a.k.a. conjugative transposons, are mobile genetic elements involved in many biological processes, including pathogenesis, symbiosis and the spread of antibiotic resistance. Unlike conjugative plasmids that are extra-chromosomal and replicate autonomously, ICEs are integrat...

journal_title：Molecular microbiology

authors： Lee CA,Babic A,Grossman AD

更新日期：2010-01-01 00:00:00

abstract：:ecs is a three-cistron operon of Bacillus subtilis, encoding proteins with similarity to the ATPase (EcsA) and hydrophobic components (EcsB) of ABC transporters. The ecsA26 point mutation was shown to cause a strong processing defect of a secreted alpha-amylase precursor (preAmyQ) and of three other exoproteins. North...

journal_title：Molecular microbiology

authors： Leskelä S,Wahlström E,Hyyryläinen HL,Jacobs M,Palva A,Sarvas M,Kontinen VP

更新日期：1999-01-01 00:00:00

abstract：:Initiation and re-initiation of chromosomal DNA replication in bacteria rely on divergent multiprotein assemblies, which direct the functional delivery of the replicative helicase on single-stranded DNA (ssDNA) at specific sites. These two processes are triggered either at the single chromosomal origin oriC or at arre...

journal_title：Molecular microbiology

authors： Bruand C,Velten M,McGovern S,Marsin S,Sérèna C,Ehrlich SD,Polard P

更新日期：2005-02-01 00:00:00

abstract：:The td group I intron interrupting the thymidylate synthase (TS) gene of phage T4 is a mobile intron that encodes the homing endonuclease I-TevI. Efficient RNA splicing of the intron is required to restore function of the TS gene, while expression of I-TevI from within the intron is required to initiate intron mobilit...

journal_title：Molecular microbiology

authors： Gibb EA,Edgell DR

更新日期：2010-10-01 00:00:00

abstract：:The natural resistance of Mycobacterium abscessus to most commonly available antibiotics seriously limits chemotherapeutic treatment options, which is particularly challenging for cystic fibrosis patients infected with this rapid-growing mycobacterium. New drugs with novel molecular targets are urgently needed against...

journal_title：Molecular microbiology

authors： Dupont C,Viljoen A,Dubar F,Blaise M,Bernut A,Pawlik A,Bouchier C,Brosch R,Guérardel Y,Lelièvre J,Ballell L,Herrmann JL,Biot C,Kremer L

更新日期：2016-08-01 00:00:00

abstract：:In Saccharomyces cerevisiae, glucose activation of cAMP synthesis requires both the presence of the G-protein-coupled receptor (GPCR) system, Gpr1-Gpa2, and uptake and phosphorylation of the sugar. In a hxt-null strain that lacks all physiologically important glucose carriers, glucose transport as well as glucose-indu...

journal_title：Molecular microbiology

authors： Rolland F,De Winde JH,Lemaire K,Boles E,Thevelein JM,Winderickx J

更新日期：2000-10-01 00:00:00

abstract：:The Yersinia Ysc apparatus is made of more than 20 proteins, 11 of which have homologues in many type III systems. Here, we characterize YscP from Yersinia enterocolitica. This 515-residue protein has a high proline content, a large tandem repetition and a slow migration in SDS-PAGE. Unlike the products of neighbourin...

journal_title：Molecular microbiology

authors： Stainier I,Bleves S,Josenhans C,Karmani L,Kerbourch C,Lambermont I,Tötemeyer S,Boyd A,Cornelis GR

更新日期：2000-09-01 00:00:00

abstract：:The archaeal transcription apparatus is chimeric: its core components (RNA polymerase and basal factors) closely resemble those of eukaryotic RNA polymerase II, but the putative archaeal transcriptional regulators are overwhelmingly of bacterial type. Particular interest attaches to how these bacterial-type effectors,...

journal_title：Molecular microbiology

authors： Ouhammouch M,Langham GE,Hausner W,Simpson AJ,El-Sayed NM,Geiduschek EP

更新日期：2005-05-01 00:00:00

abstract：:The genes of nicotine dehydrogenase (NDH) were identified, cloned and sequenced from the catabolic plasmid pAO1 of Arthrobacter nicotinovorans. In immediate proximity to this gene cluster is the beginning of the 6-hydroxy-L-niotine oxidase (6-HLNO) gene. NDH is composed of three subunits (A, B and C) of M(r) 30,011, 1...

journal_title：Molecular microbiology

authors： Grether-Beck S,Igloi GL,Pust S,Schilz E,Decker K,Brandsch R

更新日期：1994-09-01 00:00:00

abstract：:Whereas tetrahydrofolate is an essential cofactor in all bacteria, the gene that encodes the enzyme dihydrofolate reductase (DHFR) could not be identified in many of the bacteria whose genomes have been entirely sequenced. In this communication we show that the halophilic archaea Halobacterium salinarum and Haloarcula...

journal_title：Molecular microbiology

authors： Levin I,Giladi M,Altman-Price N,Ortenberg R,Mevarech M

更新日期：2004-12-01 00:00:00

abstract：:The stalk is a universal structure of the large ribosomal subunit involved in the function of translation factors. The bacterial stalk is highly stable but its stability is notably reduced in eukaryotes, favouring a translation regulatory activity of this ribosomal domain, which has not been reported in prokaryotes. T...

journal_title：Molecular microbiology

authors： Santos C,Ballesta JP

更新日期：2005-10-01 00:00:00

abstract：:Erwinia chrysanthemi, a phytopathogenic bacterium, produces a protease inhibitor which is a low-molecular-weight, heat-stable protein. In addition to its action on the three E. chrysanthemi extracellular proteases A, B and C, it also strongly inhibits the 50 kD extracellular protease of Serratia marcescens. Its struct...

journal_title：Molecular microbiology

authors： Létoffé S,Delepelaire P,Wandersman C

更新日期：1989-01-01 00:00:00

abstract：:The cytokinetic apparatus of bacteria is initially formed by the polymerization of the tubulin-like FtsZ protein into a ring structure at midcell. This so-called Z-ring facilitates the recruitment of many additional proteins to the division site to form the mature divisome machine. Although the assembly pathway leadin...

journal_title：Molecular microbiology

authors： Tsang MJ,Bernhardt TG

更新日期：2015-03-01 00:00:00

abstract：:In most soft-rotting Erwinia spp., including E. carotovora subsp. carotovora strain 71 (Ecc71), production of the plant cell wall degrading enzyme pectin lyase (Pnl) is activated by DNA-damaging agents such as mitomycin C (MC). Induction of Pnl production in Ecc71 requires a functional recA gene and the rdg locus. DNA...

journal_title：Molecular microbiology

authors： Liu Y,Chatterjee A,Chatterjee AK

更新日期：1994-12-01 00:00:00