An unusual pattern of protein expression and localization of yeast alanyl-tRNA synthetase isoforms.


:Previous studies have shown that in Saccharomyces cerevisiae the mitochondrial and cytoplasmic forms of alanyl-tRNA synthetase are encoded by a single nuclear gene, ALA1, through alternative use of in-frame successive ACG triplets and a downstream AUG triplet. Here we show that despite the obvious participation of the non-AUG-initiated leader peptide in mitochondrial localization, the leader peptide per se cannot target a cytoplasmic passenger protein into mitochondria under normal conditions. Functional mapping further shows that an efficient targeting signal is composed of the leader peptide and an 18-residue sequence downstream of Met1. Consistent to this observation, overexpression of the cytoplasmic form enables it to overcome the compartmental barrier and function in the mitochondria as well, but deletion of as few as eight amino acid residues from its amino-terminus eliminates such a potential. Thus, the sequence upstream of the first in-frame AUG initiator not only carries an unusual initiation site, but also contributes to a novel pattern of protein expression and localization.


Mol Microbiol


Molecular microbiology


Huang HY,Tang HL,Chao HY,Yeh LS,Wang CC




Has Abstract


2006-04-01 00:00:00














  • Interaction of the sensor module of Mycobacterium tuberculosis H37Rv KdpD with members of the Lpr family.

    abstract::The genetic and biochemical mechanisms by which Mycobacterium tuberculosis senses and responds to the complex environment that it encounters during infection and persistence within the host remain unknown. In a number of bacterial species, the Kdp signal transduction pathway appears to be the primary response to envir...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Steyn AJ,Joseph J,Bloom BR

    更新日期:2003-02-01 00:00:00

  • The free and bound forms of Lpp occupy distinct subcellular locations in Escherichia coli.

    abstract::The lipoprotein Lpp is the most numerically abundant protein in Escherichia coli, has been investigated for over 40 years, and has served as the paradigmatic bacterial lipoprotein since its initial discovery. It exists in two distinct forms: a 'bound-form', which is covalently bound to the cell's peptidoglycan layer, ...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Cowles CE,Li Y,Semmelhack MF,Cristea IM,Silhavy TJ

    更新日期:2011-03-01 00:00:00

  • Promoter selectivity control of Escherichia coli RNA polymerase by ionic strength: differential recognition of osmoregulated promoters by E sigma D and E sigma S holoenzymes.

    abstract::Transcription in vitro of two osmoregulated promoters, for the Escherichia coli osmB and osmY genes, was analysed using two species of RNA polymerase holoenzyme reconstituted from purified core enzyme and either sigma D (sigma 70, the major sigma in exponentially growing cells) or sigma S (sigma 38, the principal sigm...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Ding Q,Kusano S,Villarejo M,Ishihama A

    更新日期:1995-05-01 00:00:00

  • Requirements for ribosomal protein S1 for translation initiation of mRNAs with and without a 5' leader sequence.

    abstract::It has previously been proposed that Escherichia coli ribosomal protein S1 is required for the translation of highly structured mRNAs. In this study, we have examined the influence of structural features at or near the start codon of different mRNAs. The requirement for ribosomal protein S1 for translation initiation ...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Tedin K,Resch A,Bläsi U

    更新日期:1997-07-01 00:00:00

  • Three-dimensional electron microscopy reconstruction and cysteine-mediated crosslinking provide a model of the type III secretion system needle tip complex.

    abstract::Type III secretion systems are found in many Gram-negative bacteria. They are activated by contact with eukaryotic cells and inject virulence proteins inside them. Host cell detection requires a protein complex located at the tip of the device's external injection needle. The Shigella tip complex (TC) is composed of I...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Cheung M,Shen DK,Makino F,Kato T,Roehrich AD,Martinez-Argudo I,Walker ML,Murillo I,Liu X,Pain M,Brown J,Frazer G,Mantell J,Mina P,Todd T,Sessions RB,Namba K,Blocker AJ

    更新日期:2015-01-01 00:00:00

  • Molecular basis of intercellular adhesion in the biofilm-forming Staphylococcus epidermidis.

    abstract::The Staphylococcus epidermidis genes icaABC are involved in the synthesis of the polysaccharide intercellular adhesin (PIA), which is located mainly on the cell surface, as shown by immunofluorescence studies with PIA-specific antiserum. PIA was shown to be a linear beta-1,6-linked glucosaminoglycan composed of at lea...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Heilmann C,Schweitzer O,Gerke C,Vanittanakom N,Mack D,Götz F

    更新日期:1996-06-01 00:00:00

  • Interaction of CodY, a novel Bacillus subtilis DNA-binding protein, with the dpp promoter region.

    abstract::The product of the codY gene is required for nutritional repression of the Bacillus subtillis dipeptide permease operon (dpp), an operon expressed at early stationary phase in nutrient-rich medium. Though unrelated to any known DNA-binding protein, CodY was shown to bind specifically to the dpp promoter region. DNase ...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Serror P,Sonenshein AL

    更新日期:1996-05-01 00:00:00

  • Extensive alanine scanning reveals protein-protein and protein-DNA interaction surfaces in the global regulator FlhD from Escherichia coli.

    abstract::FlhD and FlhC are the transcriptional activators of the flagellar regulon. The heterotetrameric complex formed by these two proteins activates the transcription of the class II flagellar genes. The flagellar regulon consists not only of flagellar genes, but also of the chemotactic genes and some receptor proteins. Rec...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Campos A,Matsumura P

    更新日期:2001-02-01 00:00:00

  • Selective and non-selective bottlenecks as drivers of the evolution of hypermutable bacterial loci.

    abstract::Bottlenecks reduce the size of the gene pool within populations of all life forms with implications for their subsequent survival. Here, we examine the effects of bottlenecks on bacterial commensal-pathogens during transmission between, and dissemination within, hosts. By reducing genetic diversity, bottlenecks may al...

    journal_title:Molecular microbiology

    pub_type: 杂志文章,评审


    authors: De Ste Croix M,Holmes J,Wanford JJ,Moxon ER,Oggioni MR,Bayliss CD

    更新日期:2020-03-01 00:00:00

  • Functional dependence on calcineurin by variants of the Saccharomyces cerevisiae vacuolar Ca2+/H+ exchanger Vcx1p.

    abstract::The Ca(2+)-dependent protein phosphatase calcineurin is an important regulator of ion transporters from many organisms, including the Saccharomyces cerevisiae vacuolar Ca(2+)/H(+) exchanger Vcx1p. In yeast and plants, cation/H(+) exchangers are important in shaping cytosolic Ca(2+) levels involved in signal transducti...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Pittman JK,Cheng NH,Shigaki T,Kunta M,Hirschi KD

    更新日期:2004-11-01 00:00:00

  • Identification of novel virulence factors associated with signal transduction pathways in Alternaria brassicicola.

    abstract::Alternaria brassicicola is an important, necrotrophic fungal pathogen that causes black spot disease on Brassicas. In order to study pathogenicity mechanisms, gene deletion mutants were generated for 21 putative regulatory genes including kinases and transcription factors subjectively selected from the annotated A. br...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Cho Y,Kim KH,La Rota M,Scott D,Santopietro G,Callihan M,Mitchell TK,Lawrence CB

    更新日期:2009-06-01 00:00:00

  • Life cycle studies of the hexose transporter of Plasmodium species and genetic validation of their essentiality.

    abstract::A Plasmodium falciparum hexose transporter (PfHT) has previously been shown to be a facilitative glucose and fructose transporter. Its expression in Xenopus laevis oocytes and the use of a glucose analogue inhibitor permitted chemical validation of PfHT as a novel drug target. Following recent re-annotations of the P....

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Slavic K,Straschil U,Reininger L,Doerig C,Morin C,Tewari R,Krishna S

    更新日期:2010-03-01 00:00:00

  • Alteration in glycerol and metalloid permeability by a single mutation in the extracellular C-loop of Leishmania major aquaglyceroporin LmAQP1.

    abstract::The Leishmania major aquaglyceroporin, LmAQP1, is responsible for the transport of antimonite [Sb(III)], an activated form of Pentostam or Glucantime. Downregulation of LmAQP1 provides resistance to trivalent antimony compounds and increased expression of LmAQP1 in drug-resistant parasites can reverse the resistance. ...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Uzcategui NL,Zhou Y,Figarella K,Ye J,Mukhopadhyay R,Bhattacharjee H

    更新日期:2008-12-01 00:00:00

  • Identification of MrpI as the sole recombinase that regulates the phase variation of MR/P fimbria, a bladder colonization factor of uropathogenic Proteus mirabilis.

    abstract::Proteus mirabilis is a common cause of urinary tract infection (UTI) in individuals with structural abnormalities or long-term catheterization. The expression of mannose-resistant/Proteus-like (MR/P) fimbria is phase variable because of the inversion of a 251 bp DNA fragment that carries the promoter for the mrp opero...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Li X,Lockatell CV,Johnson DE,Mobley HL

    更新日期:2002-08-01 00:00:00

  • Mutagenesis, cloning and complementation analysis of C4-dicarboxylate transport genes from Rhodobacter capsulatus.

    abstract::Transposon mutagenesis was used to isolate insertion mutants of the photosynthetic bacterium Rhodobacter capsulatus which were unable to grow under aerobic conditions in the dark on malate, succinate or fumarate as sole carbon sources. Of five mutants isolated, all were deficient in C4-dicarboxylate transport. However...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Hamblin MJ,Shaw JG,Curson JP,Kelly DJ

    更新日期:1990-09-01 00:00:00

  • The purified SoxABCD quinol oxidase complex of Sulfolobus acidocaldarius contains a novel haem.

    abstract::A respiratory quinol oxidase complex that is encoded by the soxABCD operon has been purified from the thermoacidophilic archaeon Sulfolobus acidocaldarius. The enzyme was solubilized with dodecyl maltoside and purified in the presence of this detergent and ethylene glycol. The complex is hydrodynamically homogeneous a...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Lübben M,Warne A,Albracht SP,Saraste M

    更新日期:1994-07-01 00:00:00

  • Cdv-based cell division and cell cycle organization in the thaumarchaeon Nitrosopumilus maritimus.

    abstract::Cell division is mediated by different mechanisms in different evolutionary lineages. While bacteria and euryarchaea utilize an FtsZ-based mechanism, most crenarchaea divide using the Cdv system, related to the eukaryotic ESCRT-III machinery. Intriguingly, thaumarchaeal genomes encode both FtsZ and Cdv protein homolog...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Pelve EA,Lindås AC,Martens-Habbena W,de la Torre JR,Stahl DA,Bernander R

    更新日期:2011-11-01 00:00:00

  • tRNAPro -mediated downregulation of elongation factor P is required for mgtCBR expression during Salmonella infection.

    abstract::Bacterial ribosome requires elongation factor P to translate fragments harbouring consecutive proline codons. Given the abundance of ORFs with potential EF-P regulated sites, EF-P was assumed to be constitutively expressed. Here, we report that the intracellular pathogen Salmonella enterica serovar Typhimurium decreas...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Nam D,Choi E,Shin D,Lee EJ

    更新日期:2016-10-01 00:00:00

  • Buffering of stable RNA promoter activity against DNA relaxation requires a far upstream sequence.

    abstract::The stable RNA promoters of Escherichia coli are exquisitely sensitive to variations in the superhelical density of DNA. Previously, we have shown that binding of the DNA architectural protein FIS at the upstream activating sequences (UASs) of stable RNA promoters prevents the transcription complexes from inactivation...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Rochman M,Blot N,Dyachenko M,Glaser G,Travers A,Muskhelishvili G

    更新日期:2004-07-01 00:00:00

  • Lon protease inactivation, or translocation of the lon gene, potentiate bacterial evolution to antibiotic resistance.

    abstract::Previous work demonstrated that selection for Escherichia coli mutants with low antibiotic resistance frequently resulted in co-selection of lon mutations and that lon(-) mutants evolved higher-level resistance faster than a lon(+) strain. Here we show that lon mutation causes a very low multidrug resistance by induci...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Nicoloff H,Andersson DI

    更新日期:2013-12-01 00:00:00

  • Ferric uptake regulator mutants of Pseudomonas aeruginosa with distinct alterations in the iron-dependent repression of exotoxin A and siderophores in aerobic and microaerobic environments.

    abstract::Because the ferric uptake regulator (fur) appears to be an essential gene in Pseudomonas aeruginosa, resistance to manganese was used as an enrichment to isolate strains carrying point mutations in the fur gene in order to assess its role in the co-ordinate expression of siderophores and exotoxin A (ETA). This report ...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Barton HA,Johnson Z,Cox CD,Vasil AI,Vasil ML

    更新日期:1996-09-01 00:00:00

  • Bre1 and Ubp8 regulate H2B mono-ubiquitination and the reversible yeast-hyphae transition in Candida albicans.

    abstract::The reversible yeast-hyphae transition of the human fungal pathogen Candida albicans is tightly linked to its pathogenicity. In this study, we show that histone H2B mono-ubiquitination (H2Bub) at lysine 123 was maintained at a low level in the yeast state, whereas it increased significantly during yeast-to-hyphae tran...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Zhu W,Fan X,Zhao Q,Xu Y,Wang X,Chen J

    更新日期:2020-10-03 00:00:00

  • Structural insights into the enzyme catalysis from comparison of three forms of dissimilatory sulphite reductase from Desulfovibrio gigas.

    abstract::The crystal structures of two active forms of dissimilatory sulphite reductase (Dsr) from Desulfovibrio gigas, Dsr-I and Dsr-II, are compared at 1.76 and 2.05 Å resolution respectively. The dimeric α2β2γ2 structure of Dsr-I contains eight [4Fe-4S] clusters, two saddle-shaped sirohaems and two flat sirohydrochlorins. I...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Hsieh YC,Liu MY,Wang VC,Chiang YL,Liu EH,Wu WG,Chan SI,Chen CJ

    更新日期:2010-12-01 00:00:00

  • Characterization of an Escherichia coli rotA mutant, affected in periplasmic peptidyl-prolyl cis/trans isomerase.

    abstract::The rotA gene of Escherichia coli encodes a peptidyl-prolyl cis/trans isomerase (PPIase), which is supposed to catalyse protein folding in the periplasm. To investigate the importance of the enzyme, the rotA gene was cloned and a chromosomal deletion mutant was created. The rotA mutant was normally viable. No residual...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Kleerebezem M,Heutink M,Tommassen J

    更新日期:1995-10-01 00:00:00

  • The Crc protein participates in down-regulation of the Lon gene to promote rhamnolipid production and rhl quorum sensing in Pseudomonas aeruginosa.

    abstract::Rhamnolipid acts as a virulence factor during Pseudomonas aeruginosa infection. Here, we show that deletion of the catabolite repression control (crc) gene in P. aeruginosa leads to a rhamnolipid-negative phenotype. This effect is mediated by the down-regulation of rhl quorum sensing (QS). We discover that a disruptio...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Yang N,Ding S,Chen F,Zhang X,Xia Y,Di H,Cao Q,Deng X,Wu M,Wong CC,Tian XX,Yang CG,Zhao J,Lan L

    更新日期:2015-05-01 00:00:00

  • Species-specific binding of human secretory component to SpsA protein of Streptococcus pneumoniae via a hexapeptide motif.

    abstract::SpsA, a pneumococcal surface protein belonging to the family of choline-binding proteins, interacts specifically with secretory immunglobulin A (SIgA) via the secretory component (SC). SIgA and free SC from mouse, rat, rabbit and guinea-pig failed to interact with SpsA indicating species-specific binding to human SIgA...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Hammerschmidt S,Tillig MP,Wolff S,Vaerman JP,Chhatwal GS

    更新日期:2000-05-01 00:00:00

  • Targeting and sequestration of truncated Pfs230 in an intraerythrocytic compartment during Plasmodium falciparum gametocytogenesis.

    abstract::For malaria to be transmitted, the Plasmodium falciparum parasite must invade an erythrocyte and undergo gametocytogenesis. When mature intraerythrocytic gametocytes are taken up in a blood meal by a mosquito they emerge as gametes and, once fertilized, continue to differentiate into infectious sporozoites. One of the...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Eksi S,Stump A,Fanning SL,Shenouda MI,Fujioka H,Williamson KC

    更新日期:2002-06-01 00:00:00

  • Nitric oxide-sensitive and -insensitive interaction of Bacillus subtilis NsrR with a ResDE-controlled promoter.

    abstract::NsrR is a nitric oxide (NO)-sensitive transcription repressor that controls NO metabolism in a wide range of bacteria. In Bacillus subtilis, NsrR represses transcription of the nitrite reductase (nasDEF) genes that are under positive control of the ResD-ResE two-component signal transduction system. Derepression is ac...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Kommineni S,Yukl E,Hayashi T,Delepine J,Geng H,Moënne-Loccoz P,Nakano MM

    更新日期:2010-12-01 00:00:00

  • Rpc25, a conserved RNA polymerase III subunit, is critical for transcription initiation.

    abstract::Rpc25 is a strongly conserved subunit of RNA polymerase III with homology to Rpa43 in RNA polymerase I, Rpb7 in RNA polymerase II and the archaeal RpoE subunit. A central domain of Rpc25 can replaced the corresponding region of Rpb7 with little or no growth defect, underscoring the functional relatedness of these prot...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Zaros C,Thuriaux P

    更新日期:2005-01-01 00:00:00

  • Evidence that trypanothione reductase is an essential enzyme in Leishmania by targeted replacement of the tryA gene locus.

    abstract::Trypanothione reductase (TR), a flavoprotein oxidoreductase central to the unique thiol-redox system that operates in trypanosomatid protozoa, has been proposed as a potential target for the chemotherapy of trypanosomatid infections. In this study, targeted gene replacement was used to obtain evidence that TR is an es...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Tovar J,Wilkinson S,Mottram JC,Fairlamb AH

    更新日期:1998-07-01 00:00:00