Translation initiation in Escherichia coli: sequences within the ribosome-binding site.

abstract：:The ro mutants of Neurospora crassa are defective in nuclear migration and hyphal morphogenesis. Several of the ro loci have recently been shown to encode components of the dynein/dynactin motor complex. Here we report on the cloning and characterization of the ro-2 gene which codes for a novel 80 kDa protein that has...

journal_title：Molecular microbiology

authors： Vierula PJ,Mais JM

更新日期：1997-04-01 00:00:00

abstract：:Small regulatory RNAs (sRNAs) are well known to command bacterial protein synthesis by modulating the translation and decay of target mRNAs. Most sRNAs are specifically regulated by a cognate transcription factor under certain growth or stress conditions. Investigations of the conserved Hfq-dependent MicM sRNA in Esch...

journal_title：Molecular microbiology

authors： Vogel J

更新日期：2009-09-01 00:00:00

abstract：:We have identified gene fusions of polyamine biosynthetic enzymes S-adenosylmethionine decarboxylase (AdoMetDC, speD) and aminopropyltransferase (speE) orthologues in diverse bacterial phyla. Both domains are functionally active and we demonstrate the novel de novo synthesis of the triamine spermidine from the diamine...

journal_title：Molecular microbiology

authors： Green R,Hanfrey CC,Elliott KA,McCloskey DE,Wang X,Kanugula S,Pegg AE,Michael AJ

更新日期：2011-08-01 00:00:00

abstract：:Streptomyces scabies is the best studied of those streptomycetes that cause an economically important disease known as potato scab. The phytotoxin thaxtomin is made exclusively by these pathogens and is required for virulence. Here we describe regulation of thaxtomin biosynthesis by TxtR, a member of the AraC/XylS fam...

journal_title：Molecular microbiology

authors： Joshi MV,Bignell DR,Johnson EG,Sparks JP,Gibson DM,Loria R

更新日期：2007-11-01 00:00:00

abstract：:Aspergillus fumigatus, an important human nosocomial pathogen, is resistant to sordarin derivatives, a new family of antifungals that inhibit protein synthesis by interaction with the EF-2-ribosomal stalk complex. To explore the role of the A. fumigatus ribosome in the resistance mechanism, the fungal stalk proteins w...

journal_title：Molecular microbiology

authors： Santos C,Ballesta JP

更新日期：2002-01-01 00:00:00

abstract：:Expression of the Aspergillus nidulans 22 kDa endoxylanase gene, xlnA, is controlled by at least three mechanisms: specific induction by xylan or xylose; carbon catabolite repression (CCR); and regulation by ambient pH. Deletion analysis of xlnA upstream sequences has identified two positively acting regions: one that...

journal_title：Molecular microbiology

authors： Orejas M,MacCabe AP,Pérez González JA,Kumar S,Ramón D

更新日期：1999-01-01 00:00:00

abstract：:To study the role of cell division in the process of nucleoid segregation, we measured the DNA content of individual nucleoids in isogenic Escherichia coli cell division mutants by image cytometry. In pbpB(Ts) and ftsZ strains growing as filaments at 42 degrees C, nucleoids contained, on average, more than two chromos...

journal_title：Molecular microbiology

authors： Huls PG,Vischer NO,Woldringh CL

更新日期：1999-09-01 00:00:00

abstract：:Renewed interest in gene amplification stems from its importance in evolution and a variety of medical problems ranging from drug resistance to cancer. However, amplified DNA segments (amplicons) are not fully characterized in any organism. Here we report a novel Acinetobacter baylyi system for genome-wide studies. Am...

journal_title：Molecular microbiology

authors： Seaton SC,Elliott KT,Cuff LE,Laniohan NS,Patel PR,Neidle EL

更新日期：2012-02-01 00:00:00

abstract：:Escherichia coli FtsN is a bitopic membrane protein that is essential for triggering active cell constriction. A small periplasmic subdomain ((E) FtsN) is required and sufficient for function, but its mechanism of action is unclear. We isolated extragenic (E) FtsN*-suppressing mutations that restore division in cells ...

journal_title：Molecular microbiology

authors： Liu B,Persons L,Lee L,de Boer PA

更新日期：2015-03-01 00:00:00

abstract：:In F. graminearum, the transcription factor TRI6 positively regulates the trichothecene biosynthetic gene cluster (BGC) leading to the production of the secondary metabolite 15-acetyl deoxynivalenol. Secondary metabolites are not essential for survival, instead, they enable the pathogen to successfully infect its host...

journal_title：Molecular microbiology

authors： Shostak K,Bonner C,Sproule A,Thapa I,Shields SWJ,Blackwell B,Vierula J,Overy D,Subramaniam R

更新日期：2020-10-01 00:00:00

abstract：:A transposon mutant of Escherichia coli 5K was isolated which reduced 10- to 50-fold the secreted extracellular haemolytic activity of cells carrying the complete hlyCABD operon while leaving unaffected the intracellular haemolytic activity and the levels of intracellular and extracellular haemolysin protein, HlyA. Th...

journal_title：Molecular microbiology

authors： Stanley PL,Diaz P,Bailey MJ,Gygi D,Juarez A,Hughes C

更新日期：1993-11-01 00:00:00

abstract：:Agrobacterium tumefaciens is a plant pathogen that persists as surface-associated populations on plants or soil particles. A genetic screen for A. tumefaciens mutants deficient for surface interactions identified a mutant that forms thin, sparsely populated biofilms, but is proficient for initial attachment. The mutan...

journal_title：Molecular microbiology

authors： Ramey BE,Matthysse AG,Fuqua C

更新日期：2004-06-01 00:00:00

abstract：:The earliest event in bacterial cell division is the formation of a Z ring, composed of the tubulin-like FtsZ protein, at the division site at midcell. This ring then recruits several other division proteins and together they drive the formation of a division septum between two replicated chromosomes. Here we show tha...

journal_title：Molecular microbiology

authors： Peters PC,Migocki MD,Thoni C,Harry EJ

更新日期：2007-04-01 00:00:00

abstract：:Trans-acting small regulatory RNAs (sRNAs) are key players in the regulation of gene expression in bacteria. There are hundreds of different sRNAs in a typical bacterium, which in contrast to eukaryotic microRNAs are more heterogeneous in length, sequence composition, and secondary structure. The vast majority of sRNA...

journal_title：Molecular microbiology

authors： Georg J,Lalaouna D,Hou S,Lott SC,Caldelari I,Marzi S,Hess WR,Romby P

更新日期：2020-03-01 00:00:00

abstract：:Kinetoplastid topoisomerase IB is an unusual bisubunit enzyme where reconstitution of the large (LdTOPIL or L) and small (LdTOPIS or S) subunits shows functional activity. It is yet to be deciphered whether one subunit or both navigate the heterodimer to its cellular DNA targets. Tethering a specific DNA-binding prote...

journal_title：Molecular microbiology

authors： BoseDasgupta S,Ganguly A,Das BB,Roy A,Khalkho NV,Majumder HK

更新日期：2008-01-01 00:00:00

abstract：:The analysis of completed archaeal genome sequences led to the identification of a set of approximately 10-20 genes whose protein products were inferred to be involved in chromosomal DNA replication. Until recently, however, little was known of the biochemical properties of these proteins. Here, I review recent progre...

journal_title：Molecular microbiology

authors： MacNeill SA

更新日期：2001-05-01 00:00:00

abstract：:Regulation of gene expression in trypanosomatid parasitic protozoa is mainly achieved posttranscriptionally. RNA-binding proteins (RBPs) associate to 3' untranslated regions in mRNAs through dedicated domains such as the RNA recognition motif (RRM). Trypanosoma cruzi UBP1 (TcUBP1) is an RRM-type RBP involved in stabil...

journal_title：Molecular microbiology

authors： Cassola A,Romaniuk MA,Primrose D,Cervini G,D'Orso I,Frasch AC

更新日期：2015-09-01 00:00:00

abstract：:Resistance to quinoline antimalarial drugs has emerged in different parts of the world and involves sets of discrete mutational changes in pfcrt and pfmdr1 in the human malaria parasite Plasmodium falciparum. To better understand how the different polymorphic haplotypes of pfmdr1 and pfcrt contribute to drug resistanc...

journal_title：Molecular microbiology

authors： Sanchez CP,Mayer S,Nurhasanah A,Stein WD,Lanzer M

更新日期：2011-11-01 00:00:00

abstract：:Helicobacter pylori is anomalous among non nitrogen-fixing bacteria in containing an incomplete NIF system for Fe-S cluster assembly comprising two essential proteins, NifS (cysteine desulfurase) and NifU (scaffold protein). Although nifU deletion strains cannot be obtained via the conventional gene replacement, a Nif...

journal_title：Molecular microbiology

authors： Benoit SL,Holland AA,Johnson MK,Maier RJ

更新日期：2018-05-01 00:00:00

abstract：:Previous work demonstrated that selection for Escherichia coli mutants with low antibiotic resistance frequently resulted in co-selection of lon mutations and that lon(-) mutants evolved higher-level resistance faster than a lon(+) strain. Here we show that lon mutation causes a very low multidrug resistance by induci...

journal_title：Molecular microbiology

authors： Nicoloff H,Andersson DI

更新日期：2013-12-01 00:00:00

abstract：:Each bacterium has to co-ordinate its growth with division to ensure genetic stability of the population. Consequently, cell division and growth are tightly regulated phenomena, albeit different bacteria utilise one of several alternative regulatory mechanisms to maintain control. Here we consider GpsB, which is linke...

journal_title：Molecular microbiology

authors： Rismondo J,Cleverley RM,Lane HV,Großhennig S,Steglich A,Möller L,Mannala GK,Hain T,Lewis RJ,Halbedel S

更新日期：2016-03-01 00:00:00

abstract：:RNase E autoregulates its production in Escherichia coli by governing the decay rate of rne (RNase E) mRNA. It does so by a mechanism that is dependent in part on hp2, a cis-acting stem-loop within the rne 5' untranslated region. In principle, hp2 could function either as a cleavage site for RNase E or as a binding si...

journal_title：Molecular microbiology

authors： Schuck A,Diwa A,Belasco JG

更新日期：2009-04-01 00:00:00

abstract：:Sinorhizobium meliloti is a soil bacterium that invades the root nodules it induces on Medicago sativa, whereupon it undergoes an alteration of its cell cycle and differentiates into nitrogen-fixing, elongated and polyploid bacteroid with higher membrane permeability. In Caulobacter crescentus, a related alphaproteoba...

journal_title：Molecular microbiology

authors： Pini F,Frage B,Ferri L,De Nisco NJ,Mohapatra SS,Taddei L,Fioravanti A,Dewitte F,Galardini M,Brilli M,Villeret V,Bazzicalupo M,Mengoni A,Walker GC,Becker A,Biondi EG

更新日期：2013-10-01 00:00:00

abstract：:Superoxide dismutase, catalase, glutathione peroxidase and peroxiredoxins form an antioxidant network protecting cells against reactive oxygen species (ROS). Catalase is a potent H2O2-detoxifying enzyme, which is unexpectedly absent in some members of the Kinetoplastida and Apicomplexa, but present in Toxoplasma gondi...

journal_title：Molecular microbiology

authors： Ding M,Kwok LY,Schlüter D,Clayton C,Soldati D

更新日期：2004-01-01 00:00:00

abstract：:Lactose is an abundant dietary carbohydrate metabolized by the dental pathogen Streptococcus mutans. Lactose metabolism presents both classic diauxic behaviors and long-term memory, where the bacteria can pause for >11 h before initiating growth on lactose. Here, we explored mechanisms contributing to unusual aspects ...

journal_title：Molecular microbiology

authors： Zeng L,Burne RA

更新日期：2020-09-03 00:00:00

abstract：:The mechanisms of transcription repression and derepression in vivo are not fully understood. We have obtained evidence that begins to clarify the minimum requirements for counteracting nucleosomal repression in vivo. Location of the TATA element near the nucleosome dyad does not block RNA polymerase II transcription ...

journal_title：Molecular microbiology

authors： Moss DR,Laybourn PJ

更新日期：2000-06-01 00:00:00

abstract：:Helicobacter pylori colonizes the human stomach and is a potential cause of peptic ulceration or gastric adenocarcinoma. H. pylori secretes a pore-forming toxin known as vacuolating cytotoxin A (VacA). The 88 kDa secreted VacA protein, composed of an N-terminal p33 domain and a C-terminal p55 domain, assembles into wa...

journal_title：Molecular microbiology

authors： Pyburn TM,Foegeding NJ,González-Rivera C,McDonald NA,Gould KL,Cover TL,Ohi MD

更新日期：2016-10-01 00:00:00

abstract：:Chromalveolates like the diatom Phaeodactylum tricornutum arose through the uptake of a red alga by a phagotrophic protist, a process termed secondary endosymbiosis. In consequence, the plastids are surrounded by two additional membranes compared with primary plastids. This plastid morphology poses additional barriers...

journal_title：Molecular microbiology

authors： Hempel F,Felsner G,Maier UG

更新日期：2010-05-01 00:00:00

abstract：:OmpR activates expression of the two-component regulatory system located on Salmonella pathogenicity island 2 (SPI-2) that controls the expression of a type III secretion system, as well as many other genes required for systemic infection in mice. Measurements of SsrA and SsrB protein levels under different growth con...

journal_title：Molecular microbiology

authors： Feng X,Walthers D,Oropeza R,Kenney LJ

更新日期：2004-11-01 00:00:00

abstract：:Strains of Escherichia coli have been produced which express very high levels of the tRNA(1Leu) isoacceptor. This was accomplished by transforming cells with plasmids containing the leuV operon which encodes three copies of the tRNA(1Leu) gene. Most transformants grew very slowly and exhibited a 15-fold increase in ce...

journal_title：Molecular microbiology

authors： Wahab SZ,Rowley KO,Holmes WM

更新日期：1993-01-01 00:00:00