当前位置： SCI文献检索 > MOLECULAR MICROBIOLOGY期刊下所有文献 > Independent evolutionary origins of functional polyamine biosynthetic enzyme fusions catalysing de novo diamine to triamine formation.

Independent evolutionary origins of functional polyamine biosynthetic enzyme fusions catalysing de novo diamine to triamine formation.

Abstract：

:We have identified gene fusions of polyamine biosynthetic enzymes S-adenosylmethionine decarboxylase (AdoMetDC, speD) and aminopropyltransferase (speE) orthologues in diverse bacterial phyla. Both domains are functionally active and we demonstrate the novel de novo synthesis of the triamine spermidine from the diamine putrescine by fusion enzymes from β-proteobacterium Delftia acidovorans and δ-proteobacterium Syntrophus aciditrophicus, in a ΔspeDE gene deletion strain of Salmonella enterica sv. Typhimurium. Fusion proteins from marine α-proteobacterium Candidatus Pelagibacter ubique, actinobacterium Nocardia farcinica, chlorobi species Chloroherpeton thalassium, and β-proteobacterium D. acidovorans each produce a different profile of non-native polyamines including sym-norspermidine when expressed in Escherichia coli. The different aminopropyltransferase activities together with phylogenetic analysis confirm independent evolutionary origins for some fusions. Comparative genomic analysis strongly indicates that gene fusions arose by merger of adjacent open reading frames. Independent fusion events, and horizontal and vertical gene transfer contributed to the scattered phyletic distribution of the gene fusions. Surprisingly, expression of fusion genes in E. coli and S. Typhimurium revealed novel latent spermidine catabolic activity producing non-native 1,3-diaminopropane in these species. We have also identified fusions of polyamine biosynthetic enzymes agmatine deiminase and N-carbamoylputrescine amidohydrolase in archaea, and of S-adenosylmethionine decarboxylase and ornithine decarboxylase in the single-celled green alga Micromonas.

journal_name

Mol Microbiol

journal_title

Molecular microbiology

authors

Green R,Hanfrey CC,Elliott KA,McCloskey DE,Wang X,Kanugula S,Pegg AE,Michael AJ

doi

10.1111/j.1365-2958.2011.07757.x

subject

Has Abstract

pub_date

2011-08-01 00:00:00

pages

1109-24

issue

4

eissn

0950-382X

issn

1365-2958

journal_volume

81

pub_type

杂志文章

相关文献

MOLECULAR MICROBIOLOGY文献大全
  • An alternative pathway for reduced folate biosynthesis in bacteria and halophilic archaea.

    abstract：:Whereas tetrahydrofolate is an essential cofactor in all bacteria, the gene that encodes the enzyme dihydrofolate reductase (DHFR) could not be identified in many of the bacteria whose genomes have been entirely sequenced. In this communication we show that the halophilic archaea Halobacterium salinarum and Haloarcula...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2958.2004.04339.x

    authors： Levin I,Giladi M,Altman-Price N,Ortenberg R,Mevarech M

    更新日期：2004-12-01 00:00:00

  • The novel phosphatase RosC catalyzes the last unknown step of roseoflavin biosynthesis in Streptomyces davaonensis.

    abstract：:The bacterium Streptomyces davaonensis produces the antibiotic roseoflavin, which is a riboflavin (vitamin B2 ) analog. The key enzyme of roseoflavin biosynthesis is the 8-demethyl-8-amino-riboflavin-5'-phosphate (AFP) synthase RosB which synthesizes AFP from riboflavin-5'-phosphate. AFP is not a substrate for the las...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1111/mmi.14567

    authors： Schneider C,Konjik V,Kißling L,Mack M

    更新日期：2020-10-01 00:00:00

  • Role of the Escherichia coli SbmA in the antimicrobial activity of proline-rich peptides.

    abstract：:In contrast to many antimicrobial peptides, members of the proline-rich group of antimicrobial peptides inactivate Gram-negative bacteria by a non-lytic mechanism. Several lines of evidence indicate that they are internalized into bacteria and their activity mediated by interaction with unknown cellular components. Wi...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2958.2007.05903.x

    authors： Mattiuzzo M,Bandiera A,Gennaro R,Benincasa M,Pacor S,Antcheva N,Scocchi M

    更新日期：2007-10-01 00:00:00

  • Identification and characterization of the pupB gene encoding an inducible ferric-pseudobactin receptor of Pseudomonas putida WCS358.

    abstract：:Pseudomonas putida WCS358 can transport iron complexed to a wide variety of pseudobactins produced by other Pseudomonas strains. The pupB gene encoding an outer membrane ferric-pseudobactin receptor was isolated from a genomic library of P. putida WCS358. The PupB receptor facilitated iron transport via two distinct h...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2958.1993.tb01603.x

    authors： Koster M,van de Vossenberg J,Leong J,Weisbeek PJ

    更新日期：1993-05-01 00:00:00

  • Reverse transcriptase in bacteria.

    abstract：:Reverse transcriptase, discovered in 1970 in retroviruses, has until recently been found only in eukaryotic organisms. Recently it was shown to occur in two groups of bacteria: myxobacteria and Escherichia coli. The gene for reverse transcriptase is part of a chromosomal genetic element that codes for the production o...

    journal_title：Molecular microbiology

    pub_type： 杂志文章,评审

    doi：10.1111/j.1365-2958.1989.tb00264.x

    authors： Lim D,Maas WK

    更新日期：1989-08-01 00:00:00

  • Analysis of the distribution and evolution of the ATP-dependent DNA ligases of bacteria delineates a distinct phylogenetic group 'Lig E'.

    abstract：:Prior to the discovery of a minimal ATP-dependent DNA ligase in Haemophilus influenzae, bacteria were thought to only possess a NAD-dependent ligase, which was involved in sealing of Okazaki fragments. We now know that a diverse range of bacterial species possess up to six of these accessory bacterial ATP-dependent DN...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1111/mmi.13229

    authors： Williamson A,Hjerde E,Kahlke T

    更新日期：2016-01-01 00:00:00

  • Osmotic induction of the periplasmic trehalase in Escherichia coli K12: characterization of the treA gene promoter.

    abstract：:The Escherichia coli treA gene encodes an osmotically inducible periplasmic trehalase. A strain carrying a treA-lacZ transcriptional fusion was constructed. The beta-galactosidase activity produced in this strain growing exponentially in a medium of high osmotic pressure was 10-fold higher than that produced in a medi...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2958.1991.tb00745.x

    authors： Repoila F,Gutierrez C

    更新日期：1991-03-01 00:00:00

  • Iron-sulfur protein maturation in Helicobacter pylori: identifying a Nfu-type cluster carrier protein and its iron-sulfur protein targets.

    abstract：:Helicobacter pylori is anomalous among non nitrogen-fixing bacteria in containing an incomplete NIF system for Fe-S cluster assembly comprising two essential proteins, NifS (cysteine desulfurase) and NifU (scaffold protein). Although nifU deletion strains cannot be obtained via the conventional gene replacement, a Nif...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1111/mmi.13942

    authors： Benoit SL,Holland AA,Johnson MK,Maier RJ

    更新日期：2018-05-01 00:00:00

  • ahpC, a gene involved in isoniazid resistance of the Mycobacterium tuberculosis complex.

    abstract：:A gene conferring low-level isoniazid (INH) resistance on Mycobacterium smegmatis was isolated from a cosmid library of the genome of an INH-resistant Mycobacterium bovis strain. The gene had good homology with ahpC, the product of which is a subunit of alkyl hydroperoxide reductase, and also with a family of thiol-sp...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2958.1996.449980.x

    authors： Wilson TM,Collins DM

    更新日期：1996-03-01 00:00:00

  • A nuclear protein NsiA from Epichloë festucae interacts with a MAP kinase MpkB and regulates the expression of genes required for symbiotic infection and hyphal cell fusion.

    abstract：:The endophytic fungus Epichloë festucae systemically colonizes the intercellular spaces of cool-season grasses to establish a mutualistic symbiosis. Hyphal growth of the endophyte within the host plant is tightly regulated and synchronized with the growth of the host plant. A genetic screen to identify symbiotic genes...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1111/mmi.14568

    authors： Tanaka A,Kamiya S,Ozaki Y,Kameoka S,Kayano Y,Saikia S,Akano F,Uemura A,Takagi H,Terauchi R,Maruyama JI,Hammadeh HH,Fleissner A,Scott B,Takemoto D

    更新日期：2020-10-01 00:00:00

  • Lipid domains in bacterial membranes.

    abstract：:The recent development of specific probes for lipid molecules has led to the discovery of lipid domains in bacterial membranes, that is, of membrane areas differing in lipid composition. A view of the membrane as a patchwork is replacing the assumption of lipid homogeneity inherent in the fluid mosaic model of Singer ...

    journal_title：Molecular microbiology

    pub_type： 杂志文章,评审

    doi：10.1111/j.1365-2958.2006.05317.x

    authors： Matsumoto K,Kusaka J,Nishibori A,Hara H

    更新日期：2006-09-01 00:00:00

  • Acidianus, Sulfolobus and Metallosphaera surface layers: structure, composition and gene expression.

    abstract：:The cell walls of Sulfolobales species consist of proteinaceous S-layers assembled from two polypeptides, SlaA and SlaB. We isolated the large S-layer protein of Acidianus ambivalens and both S-layer subunits of Sulfolobus solfataricus and Metallosphaera sedula, respectively. The slaAB genes, lying adjacently in the c...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2958.2009.06746.x

    authors： Veith A,Klingl A,Zolghadr B,Lauber K,Mentele R,Lottspeich F,Rachel R,Albers SV,Kletzin A

    更新日期：2009-07-01 00:00:00

  • Mismatch repair and the regulation of phase variation in Neisseria meningitidis.

    abstract：:Neisseria meningitidis controls the expression of several genes involved in host adaptation by a process known as phase variation. The phase variation frequency of haemoglobin (Hb) receptors among clinical isolates of serogroups A, B and C differed drastically, ranging from approximately 10(-6) to 10(-2) cfu-1. Freque...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2958.2001.02408.x

    authors： Richardson AR,Stojiljkovic I

    更新日期：2001-05-01 00:00:00

  • Hop-family Helicobacter outer membrane adhesins form a novel class of Type 5-like secretion proteins with an interrupted β-barrel domain.

    abstract：:The human stomach pathogen Helicobacter pyloriattaches to healthy and inflamed gastric tissue through members of a paralogous family of 'Helicobacter outer membrane proteins' (Hops), including adhesins BabA, SabA, HopQ, LabA and HopZ. Hops share a conserved 25 kDa C-terminal region that is thought to form an autotrans...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1111/mmi.14075

    authors： Coppens F,Castaldo G,Debraekeleer A,Subedi S,Moonens K,Lo A,Remaut H

    更新日期：2018-10-01 00:00:00

  • Cutting out the φC31 prophage.

    abstract：:To establish a lysogenic lifestyle, the temperate bacteriophage φC31 integrates its genome into the chromosome of its Streptomyces host, by site-specific recombination between attP (the attachment site in the phage DNA) and attB (the chromosomal attachment site). This reaction is promoted by a phage-encoded serine rec...

    journal_title：Molecular microbiology

    pub_type： 评论,杂志文章

    doi：10.1111/j.1365-2958.2011.07699.x

    authors： Stark WM

    更新日期：2011-06-01 00:00:00

  • Evidence for dynamic in vivo interconversion of the conformational states of IscU during iron-sulfur cluster biosynthesis.

    abstract：:IscU is a central component of the ISC machinery and serves as a scaffold for de novo assembly of Fe-S clusters. The dedicated chaperone system composed of the Hsp70-chaperone HscA and the J-protein cochaperone HscB synergistically interacts with IscU and facilitates cluster transfer from IscU to recipient apo-protein...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1111/mmi.14646

    authors： Sato S,Matsushima Y,Kanazawa M,Tanaka N,Fujishiro T,Kunichika K,Nakamura R,Tomioka H,Wada K,Takahashi Y

    更新日期：2020-11-17 00:00:00

  • Pseudo-allelic relationship between non-homologous genes concerned with biosynthesis of polyglycerol phosphate and polyribitol phosphate teichoic acids in Bacillus subtilis strains 168 and W23.

    abstract：:A 60 kbp region of the Bacillus subtilis chromosome encompassing the genes concerned with teichoic acid biosynthesis has been subjected to physical analysis. No homology was detected by Southern hybridization between DNA segments encoding the tag genes of strain 168, concerned with polyglycerol phosphate (poly(groP)) ...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2958.1989.tb00166.x

    authors： Young M,Mauël C,Margot P,Karamata D

    更新日期：1989-12-01 00:00:00

  • Molecular characterization of catalase from Bordetella pertussis: identification of the katA promoter in an upstream insertion sequence.

    abstract：:In this report we evaluate the role of catalase in the survival of Bordetella pertussis within human polymorphonuclear leukocytes (PMNs). Crude extracts of B. pertussis exhibited a single catalase activity when subjected to non-denaturing polyacrylamide gel electrophoresis and assayed for catalase activity. A plasmid ...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2958.1994.tb01272.x

    authors： DeShazer D,Wood GE,Friedman RL

    更新日期：1994-10-01 00:00:00

  • Regulated, sequential processing by multiple proteases is required for proper maturation and release of Bordetella filamentous hemagglutinin.

    abstract：:Filamentous hemagglutinin (FHA) is a critically important virulence factor produced by Bordetella species that cause respiratory infections in humans and other animals. It is also a prototypical member of the widespread two partner secretion (TPS) pathway family of proteins. First synthesized as a ~370 kDa protein cal...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1111/mmi.14318

    authors： Nash ZM,Cotter PA

    更新日期：2019-09-01 00:00:00

  • Regulation of competence for genetic transformation in Streptococcus pneumoniae: expression of dpnA, a late competence gene encoding a DNA methyltransferase of the DpnII restriction system.

    abstract：:The chromosomal DpnII gene cassette of Streptococcus pneumoniae encodes two methyltransferases and an endonuclease. One methyltransferase acts on double-stranded and the other on single-stranded DNA. Two mRNAs are transcribed from the cassette. One, a SigA promoter transcript, includes all three genes; the other inclu...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2958.2000.01777.x

    authors： Lacks SA,Ayalew S,de la Campa AG,Greenberg B

    更新日期：2000-03-01 00:00:00

  • In vitro reconstitution of a trimeric complex of DivIB, DivIC and FtsL, and their transient co-localization at the division site in Streptococcus pneumoniae.

    abstract：:DivIB, DivIC and FtsL are bacterial proteins essential for cell division, which show interdependencies for their stabilities and localization. We have reconstituted in vitro a trimeric complex consisting of the recombinant extracellular domains of the three proteins from Streptococcus pneumoniae. The extracellular dom...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2958.2004.04408.x

    authors： Noirclerc-Savoye M,Le Gouëllec A,Morlot C,Dideberg O,Vernet T,Zapun A

    更新日期：2005-01-01 00:00:00

  • Analysis of the replicon region and identification of an rRNA operon on pBM400 of Bacillus megaterium QM B1551.

    abstract：:An 18 633 bp region containing the replicon from the approximately 53 kb pBM400 plasmid of Bacillus megaterium QM B1551 has been sequenced and characterized. This region contained a complete rRNA operon plus 10 other potential open reading frames (ORFs). The replicon consisted of an upstream promoter and three contigu...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2958.2001.02292.x

    authors： Kunnimalaiyaan M,Stevenson DM,Zhou Y,Vary PS

    更新日期：2001-02-01 00:00:00

  • Carbon catabolite repression in Aspergillus nidulans involves deubiquitination.

    abstract：:The best studied role of ubiquitination is to mark proteins for destruction by the proteasome but, in addition, it has recently been shown to promote macromolecular assembly and function, and alter protein function, thus playing a regulatory role distinct from protein degradation. Deubiquinating enzymes, the ubiquitin...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2958.2001.02474.x

    authors： Lockington RA,Kelly JM

    更新日期：2001-06-01 00:00:00

  • EthR, a repressor of the TetR/CamR family implicated in ethionamide resistance in mycobacteria, octamerizes cooperatively on its operator.

    abstract：:Ethionamide (ETH) is an important second-line antitubercular drug used for the treatment of patients infected with multidrug-resistant Mycobacterium tuberculosis. Although ETH is a structural analogue of isoniazid, only little cross-resistance to these two drugs is observed among clinical isolates. Both isoniazid and ...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2958.2003.03809.x

    authors： Engohang-Ndong J,Baillat D,Aumercier M,Bellefontaine F,Besra GS,Locht C,Baulard AR

    更新日期：2004-01-01 00:00:00

  • Interspecies recombination, and phylogenetic distortions, within the glutamine synthetase and shikimate dehydrogenase genes of Neisseria meningitidis and commensal Neisseria species.

    abstract：:Visual inspection showed clear evidence of a history of intraspecies recombinational exchanges within the neighbouring meningococcal shikimate dehydrogenase (aroE) and glutamine synthetase (glnA) genes, which was supported by the non-congruence of the trees constructed from the sequences of these genes from different ...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2958.1997.2681633.x

    authors： Zhou J,Bowler LD,Spratt BG

    更新日期：1997-02-01 00:00:00

  • Genetic ablation of a Maurer's cleft protein prevents assembly of the Plasmodium falciparum virulence complex.

    abstract：:The malaria parasite Plasmodium falciparum assembles knob structures underneath the erythrocyte membrane that help present the major virulence protein, P. falciparum erythrocyte membrane protein-1 (PfEMP1). Membranous structures called Maurer's clefts are established in the erythrocyte cytoplasm and function as sortin...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2958.2011.07740.x

    authors： Dixon MW,Kenny S,McMillan PJ,Hanssen E,Trenholme KR,Gardiner DL,Tilley L

    更新日期：2011-08-01 00:00:00

  • The use of a rare codon specifically during development?

    abstract：:A range of circumstantial evidence suggests that in Streptomyces spp., genes required for vegetative growth do not contain the leucine codon TTA. Instead, the codon seems to be confined to a few genes necessary during differentiation, when the colonies begin to produce aerial hyphae and antibiotics. Thus, mutations in...

    journal_title：Molecular microbiology

    pub_type： 杂志文章,评审

    doi：10.1111/j.1365-2958.1991.tb01845.x

    authors： Leskiw BK,Bibb MJ,Chater KF

    更新日期：1991-12-01 00:00:00

  • Four signalling domains in the hybrid histidine protein kinase RodK of Myxococcus xanthus are required for activity.

    abstract：:In prokaryotes, the principal signal transduction systems operating at the level of protein phosphorylation are the two-component systems. A number of hybrid histidine protein kinases in these systems contain several receiver domains, however, the function of these receiver domains is unknown. The RodK kinase in Myxoc...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2958.2006.05118.x

    authors： Rasmussen AA,Wegener-Feldbrügge S,Porter SL,Armitage JP,Søgaard-Andersen L

    更新日期：2006-04-01 00:00:00

  • Neisseria gonorrhoeae acquires mutations in analogous regions of gyrA and parC in fluoroquinolone-resistant isolates.

    abstract：:Neisseria gonorrhoeae homologues of gyrA and parC have been identified using hybridization probes generated from conserved regions of diverse gyrA genes. These genes have been tentatively identified as gyrA and parC, based on predicted amino acid sequence homologies to known GyrA homologues from numerous bacterial spe...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2958.1994.tb01297.x

    authors： Belland RJ,Morrison SG,Ison C,Huang WM

    更新日期：1994-10-01 00:00:00

  • Secondary metabolic gene cluster silencing in Aspergillus nidulans.

    abstract：:In contrast to most primary metabolism genes, the genes involved in secondary metabolism and certain nutrient utilization pathways are clustered in fungi. Recently a nuclear protein, LaeA, was found to be required for the transcription of several secondary metabolite gene clusters in Aspergillus nidulans. Here we show...

    journal_title：Molecular microbiology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2958.2006.05330.x

    authors： Bok JW,Noordermeer D,Kale SP,Keller NP

    更新日期：2006-09-01 00:00:00