Abstract：
:Neisseria gonorrhoeae homologues of gyrA and parC have been identified using hybridization probes generated from conserved regions of diverse gyrA genes. These genes have been tentatively identified as gyrA and parC, based on predicted amino acid sequence homologies to known GyrA homologues from numerous bacterial species and to ParC from Escherichia coli and Salmonella typhimurium. The gyrA gene maps to a physical location distant from the gyrB locus on the gonococcal chromosome, which is similar to the situation found in E. coli. The parC gene is not closely linked (i.e. greater than 9 kb) to an identifiable parE gene in N. gonorrhoeae. The gonococcal GyrA is slightly larger than its E. coli homologue and contains several small insertions near the C-terminus of the predicted open reading frame. A series of ciprofloxacin-resistant mutants were selected by passage of N. gonorrhoeae on increasing concentrations of the antibiotic. Sequential passage resulted in the selection of isolates with minimum inhibitory concentrations approximately 10,000-fold higher than the parental strain. Mutations within gyrA resulted in low to moderate levels of resistance, while strains with high-level resistance acquired analogous mutations in both gyrA and parC. Resistance mutations were readily transferred between N. gonorrhoeae strains by transformation. The frequencies of transformation, resulting in different levels of ciprofloxacin resistance, further support the notion that both gyrA and parC genes are involved in the establishment of extreme levels of ciprofloxacin resistance.
journal_name
Mol Microbioljournal_title
Molecular microbiologyauthors
Belland RJ,Morrison SG,Ison C,Huang WMdoi
10.1111/j.1365-2958.1994.tb01297.xsubject
Has Abstractpub_date
1994-10-01 00:00:00pages
371-80issue
2eissn
0950-382Xissn
1365-2958journal_volume
14pub_type
杂志文章abstract：:The stalk is a universal structure of the large ribosomal subunit involved in the function of translation factors. The bacterial stalk is highly stable but its stability is notably reduced in eukaryotes, favouring a translation regulatory activity of this ribosomal domain, which has not been reported in prokaryotes. T...
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doi：10.1111/j.1365-2958.2005.04816.x
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journal_title：Molecular microbiology
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doi：10.1046/j.1365-2958.2000.01708.x
更新日期：2000-01-01 00:00:00
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更新日期：1997-04-01 00:00:00
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pub_type： 杂志文章,评审
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更新日期：2000-07-01 00:00:00
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更新日期：2003-02-01 00:00:00
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更新日期：2000-01-01 00:00:00
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更新日期：1993-05-01 00:00:00
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journal_title：Molecular microbiology
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更新日期：1991-08-01 00:00:00
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更新日期：2007-11-01 00:00:00
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更新日期：2001-07-01 00:00:00
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更新日期：2008-09-01 00:00:00
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更新日期：2000-03-01 00:00:00
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