Abstract：
:The Escherichia coli treA gene encodes an osmotically inducible periplasmic trehalase. A strain carrying a treA-lacZ transcriptional fusion was constructed. The beta-galactosidase activity produced in this strain growing exponentially in a medium of high osmotic pressure was 10-fold higher than that produced in a medium of low osmotic pressure, demonstrating that treA transcription is osmotically inducible. treA transcriptional induction depends neither on the presence of trehalase itself nor on the synthesis of cytoplasmic trehalose which occurs in response to osmotic stress in wild-type E. coli strains. The treA promoter was identified by S1 nuclease protection. Deletion analysis demonstrated that sequences sufficient for the osmotic induction lie downstream from nucleotide -40 with respect to the transcription start. Transcription initiation at treAp required the presence of a functional sigma 70 subunit of RNA polymerase. treA expression was increased in the presence of a mutation in osmZ, which was previously identified as leading to a partially constitutive expression of the osmotically inducible proU operon.
journal_name
Mol Microbioljournal_title
Molecular microbiologyauthors
Repoila F,Gutierrez Cdoi
10.1111/j.1365-2958.1991.tb00745.xsubject
Has Abstractpub_date
1991-03-01 00:00:00pages
747-55issue
3eissn
0950-382Xissn
1365-2958journal_volume
5pub_type
杂志文章abstract：:The DNA encoding the zinc metalloprotease of Legionella pneumophila Philadelphia 1 has been isolated and expressed in Escherichia coli. This protein, which is 38,000 Daltons in size, possesses immunological and biochemical properties identical to those previously described for the purified L. pneumophila protease. Per...
journal_title：Molecular microbiology
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更新日期：1995-06-01 00:00:00
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journal_title：Molecular microbiology
pub_type： 杂志文章
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更新日期：1994-05-01 00:00:00
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更新日期：2017-03-01 00:00:00
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pub_type： 杂志文章
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更新日期：1996-06-01 00:00:00
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pub_type： 杂志文章
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更新日期：2004-02-01 00:00:00
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更新日期：1996-11-01 00:00:00
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pub_type： 杂志文章,评审
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更新日期：1991-11-01 00:00:00
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