The novel phosphatase RosC catalyzes the last unknown step of roseoflavin biosynthesis in Streptomyces davaonensis.

abstract：:Ribonucleotide reductases supply cells with their deoxyribonucleotides. Three enzyme types are known, classes I, II and III. Class II enzymes are anaerobic whereas class I enzymes are aerobic, and so class I and II enzymes are often produced by the same organism under opposing oxygen regimes. Escherichia coli contains...

journal_title：Molecular microbiology

authors： Andrews SC

更新日期：2011-04-01 00:00:00

abstract：:Malaria transmission requires that the parasites differentiate into gametocytes prior to ingestion by a mosquito during a blood meal. Once in the mosquito midgut the gametocytes emerge from red blood cells (RBCs), fertilize, develop into ookinetes and finally infectious sporozoites. Gamete surface antigen, Pfs230, is ...

journal_title：Molecular microbiology

authors： Eksi S,Czesny B,van Gemert GJ,Sauerwein RW,Eling W,Williamson KC

更新日期：2006-08-01 00:00:00

abstract：:Listeria monocytogenes is a Gram-positive intracellular bacterium responsible for severe opportunistic infections in humans and animals. Signature-tagged mutagenesis (STM) was used to identify a gene named fbpA, required for efficient liver colonization of mice inoculated intravenously. FbpA was also shown to be requi...

journal_title：Molecular microbiology

authors： Dramsi S,Bourdichon F,Cabanes D,Lecuit M,Fsihi H,Cossart P

更新日期：2004-07-01 00:00:00

abstract：:Fungi defend their ecological niche against antagonists by producing antibiosis molecules. Some of these molecules are only produced upon confrontation with the antagonist. The basidiomycete Coprinopsis cinerea induces the expression of the sesquiterpene synthase-encoding gene cop6 and its two neighboring genes coding...

journal_title：Molecular microbiology

authors： Stöckli M,Morinaka BI,Lackner G,Kombrink A,Sieber R,Margot C,Stanley CE,deMello AJ,Piel J,Künzler M

更新日期：2019-08-01 00:00:00

abstract：:Cell division is mediated by different mechanisms in different evolutionary lineages. While bacteria and euryarchaea utilize an FtsZ-based mechanism, most crenarchaea divide using the Cdv system, related to the eukaryotic ESCRT-III machinery. Intriguingly, thaumarchaeal genomes encode both FtsZ and Cdv protein homolog...

journal_title：Molecular microbiology

authors： Pelve EA,Lindås AC,Martens-Habbena W,de la Torre JR,Stahl DA,Bernander R

更新日期：2011-11-01 00:00:00

abstract：:XylR is a σ⁵⁴-dependent transcriptional factor of Pseudomonas putida that activates the Pu promoter of the TOL plasmid upon binding its natural effector, m-xylene. The search for mutants of the signal-sensing module of XylR that respond to the xenobiotic compound 2,4-dinitrotoluene recurrently yields protein variants ...

journal_title：Molecular microbiology

authors： de Las Heras A,de Lorenzo V

更新日期：2011-03-01 00:00:00

abstract：:N-terminal protein acetylation is common in eukaryotes and halophilic archaea, but very rare in bacteria. We demonstrate that some of the most abundant proteins present in the crenarchaeote Sulfolobus solfataricus, including subunits of the thermosome, proteosome and ribosome, are acetylated at the N-terminus. Modific...

journal_title：Molecular microbiology

authors： Mackay DT,Botting CH,Taylor GL,White MF

更新日期：2007-06-01 00:00:00

abstract：:The location of FtsQ, an Escherichia coli protein essential for cell division, is, under physiological conditions, in the cytoplasmic membrane facing towards the periplasmic space. An amino-proximal hydrophobic domain is required for FtsQ to reach its location and for its activity in the cell. Overexpression of modifi...

journal_title：Molecular microbiology

authors： Dopazo A,Palacios P,Sánchez M,Pla J,Vicente M

更新日期：1992-03-01 00:00:00

abstract：:Random gene tagging was used to obtain new mutants of the marine cyanobacterium, Synechococcus sp. PCC7002, with defects in the CO2-concentrating mechanism (CCM). Two of these mutants, K22 and A41, showed poor growth at limiting CO2. Isolation and sequencing of a 6. 6 kb genomic region revealed the existence of five p...

journal_title：Molecular microbiology

authors： Klughammer B,Sültemeyer D,Badger MR,Price GD

更新日期：1999-06-01 00:00:00

abstract：:Site-specific recombination by the Cre recombinase takes place at a simple DNA site (loxP), requires no additional proteins and gives topologically simple recombination products. In contrast, cer and psi sites for Xer recombination contain approximately 150 bp of accessory sequences, require accessory proteins PepA, A...

journal_title：Molecular microbiology

authors： Gourlay SC,Colloms SD

更新日期：2004-04-01 00:00:00

abstract：:Although cytoplasmic bridges between adjacent cells in the filaments of nitrogen-fixing cyanobacteria have been known for decades, the existence also of a continuous periplasm along the filaments raised the possibility that alternative modes of communication between cells could be utilized. The latter hypothesis was i...

journal_title：Molecular microbiology

authors： Haselkorn R

更新日期：2008-11-01 00:00:00

abstract：:Helicobacter pylori is a human gastric pathogen associated with gastric and duodenal ulcers as well as gastric cancer. Mounting evidence suggests this pathogen's motility is prerequisite for successful colonization of human gastric tissues. Here, we isolated an H. pylori G27 HP0518 mutant exhibiting altered motility i...

journal_title：Molecular microbiology

authors： Asakura H,Churin Y,Bauer B,Boettcher JP,Bartfeld S,Hashii N,Kawasaki N,Mollenkopf HJ,Jungblut PR,Brinkmann V,Meyer TF

更新日期：2010-12-01 00:00:00

abstract：:The bioluminescent marine bacterium Vibrio harveyi controls light production (lux) by an elaborate quorum-sensing circuit. V. harveyi produces and responds to two different autoinducer signals (AI-1 and AI-2) to modulate the luciferase structural operon (luxCDABEGH) in response to changes in cell-population density. U...

journal_title：Molecular microbiology

authors： Lilley BN,Bassler BL

更新日期：2000-05-01 00:00:00

abstract：:The 136 codon (408 bp) denA gene encoding endonuclease II (Endoll) of bacteriophage T4 was unambiguously identified through sequencing and subsequent cloning. Endoll prepared from cloned DNA through coupled in vitro transcription-translation nicked and cut DNA in vitro in a sequence-specific manner. In vitro (and in v...

journal_title：Molecular microbiology

authors： Carlson K,Kosturko LD,Nyström AC

更新日期：1999-03-01 00:00:00

abstract：:We found that mutations that increased the transcription of the mgtCBR (Mg2+ transport-related) operon conferred increased thermotolerance on this organism. The 5' leader of the mgtCBR mRNA contains two short open reading frames (ORFs), mgtM and mgtP, whose translation regulates the expression of the mgtCBR operon by ...

journal_title：Molecular microbiology

authors： Gall AR,Hegarty AE,Datsenko KA,Westerman RP,SanMiguel P,Csonka LN

更新日期：2018-08-01 00:00:00

abstract：:Ureaplasma urealyticum (U. urealyticum), belonging to the class Mollicutes, is a human pathogen colonizing the urogenital tract and causes among other things respiratory diseases in premature infants. We have studied the salvage of pyrimidine deoxynucleosides in U. urealyticum and cloned a key salvage enzyme, thymidin...

journal_title：Molecular microbiology

authors： Carnrot C,Wehelie R,Eriksson S,Bölske G,Wang L

更新日期：2003-11-01 00:00:00

abstract：:Listeriolysin O (LLO) binds to cholesterol-containing membranes in which it oligomerizes to form pores. Preincubation of the toxin with cholesterol is known to inhibit haemolysis, whereas the oxidized form of cholesterol has no inhibitory effect. Using immunoblot analyses and flow cytometry we demonstrate that preincu...

journal_title：Molecular microbiology

authors： Jacobs T,Darji A,Frahm N,Rohde M,Wehland J,Chakraborty T,Weiss S

更新日期：1998-06-01 00:00:00

abstract：:Most available knowledge on fungal arginine metabolism is derived from studies on Saccharomyces cerevisiae, in which arginine catabolism is initiated by releasing urea via the arginase reaction. Orthologues of the S. cerevisiae genes encoding the first three enzymes in the arginase pathway were cloned from Kluyveromyc...

journal_title：Molecular microbiology

authors： Romagnoli G,Verhoeven MD,Mans R,Fleury Rey Y,Bel-Rhlid R,van den Broek M,Seifar RM,Ten Pierick A,Thompson M,Müller V,Wahl SA,Pronk JT,Daran JM

更新日期：2014-07-01 00:00:00

abstract：:The archaeal transcription apparatus is chimeric: its core components (RNA polymerase and basal factors) closely resemble those of eukaryotic RNA polymerase II, but the putative archaeal transcriptional regulators are overwhelmingly of bacterial type. Particular interest attaches to how these bacterial-type effectors,...

journal_title：Molecular microbiology

authors： Ouhammouch M,Langham GE,Hausner W,Simpson AJ,El-Sayed NM,Geiduschek EP

更新日期：2005-05-01 00:00:00

abstract：:Two papers in this issue of Molecular Microbiology from Carol Gross and colleagues describe experiments that investigate how two alternative Escherichia coli sigma proteins recognize target promoter -10 regions. A combination of genetics, biochemistry and bioinformatics is used to show that determinants in two adjacen...

journal_title：Molecular microbiology

authors： Busby SJ

更新日期：2009-05-01 00:00:00

abstract：:PilO of Pseudomonas aeruginosa 1244 catalyses the attachment of an O-antigen repeating unit to the beta-carbon of the pilin C-terminal residue, a serine. The present study was conducted to locate the regions of this enzyme important in catalysis and to establish the cellular location of the pilin glycosylation reactio...

journal_title：Molecular microbiology

authors： Qutyan M,Paliotti M,Castric P

更新日期：2007-12-01 00:00:00

abstract：:A two-hybrid approach was applied to screen for proteins with the ability to interact with PHB synthase (PhaC1) of Ralstonia eutropha. The H16_A0141 gene (phaM) was identified in the majority of positive clones. PhaM (26.6 kDa) strongly interacted with PhaC1 and with phasin PhaP5 but not with PhaP1 or other PHB granul...

journal_title：Molecular microbiology

authors： Pfeiffer D,Wahl A,Jendrossek D

更新日期：2011-11-01 00:00:00

abstract：:The transport of iron complexes through outer membrane transporters from Gram-negative bacteria is highly dependent on the TonB system. Together, the three components of the system, TonB, ExbB and ExbD, energize the transport of iron complexes through the outer membrane by utilizing the proton motive force across the ...

journal_title：Molecular microbiology

authors： Garcia-Herrero A,Peacock RS,Howard SP,Vogel HJ

更新日期：2007-11-01 00:00:00

abstract：:The nucleotide sequence of a 2711bp DNA segment which contains the N-terminal coding sequence and the 5' flanking region of a crystal protein gene (bta) from Bacillus thuringiensis subsp. aizawai 7.29 has been determined. The coding region encodes an 824 amino-acid polypeptide corresponding to a carboxy-terminally tru...

journal_title：Molecular microbiology

authors： Sanchis V,Lereclus D,Menou G,Chaufaux J,Guo S,Lecadet MM

更新日期：1989-02-01 00:00:00

abstract：:Phaseolotoxin, a phytotoxin of Pseudomonas syringae pv. phaseolicola, is produced at 18 degrees C but not at 28 degrees C. Here we report that a fragment (24.4 kb) cloned from the wild-type strain, which does not harbour a gene(s) involved in phaseolotoxin biosynthesis, abolishes this thermoregulation in the wild type...

journal_title：Molecular microbiology

authors： Rowley KB,Clements DE,Mandel M,Humphreys T,Patil SS

更新日期：1993-05-01 00:00:00

abstract：:The septum-located DNA translocase, FtsK, acts to co-ordinate the late steps of Escherichia coli chromosome segregation with cell division. The FtsK gamma regulatory subdomain interacts with 8 bp KOPS DNA sequences, which are oriented from the replication origin to the terminus region (ter) in each arm of the chromoso...

journal_title：Molecular microbiology

authors： Sivanathan V,Emerson JE,Pages C,Cornet F,Sherratt DJ,Arciszewska LK

更新日期：2009-02-01 00:00:00

abstract：:Ethionamide (ETH) is an important second-line antitubercular drug used for the treatment of patients infected with multidrug-resistant Mycobacterium tuberculosis. Although ETH is a structural analogue of isoniazid, only little cross-resistance to these two drugs is observed among clinical isolates. Both isoniazid and ...

journal_title：Molecular microbiology

authors： Engohang-Ndong J,Baillat D,Aumercier M,Bellefontaine F,Besra GS,Locht C,Baulard AR

更新日期：2004-01-01 00:00:00

abstract：:A model for the topology of the PhoE porin has been proposed according to which the polypeptide traverses the outer membrane sixteen times mostly as amphipathic beta-sheets, thereby exposing eight loops at the cell surface. Until now, no evidence has been obtained for the surface exposure of the third loop. Recently, ...

journal_title：Molecular microbiology

authors： Struyvé M,Visser J,Adriaanse H,Benz R,Tommassen J

更新日期：1993-01-01 00:00:00

abstract：:We have characterized a number of mutations in fnr that interfere with FNR-dependent transcription activation at two promoters where the FNR-binding site is centred around 41 1/2 bp upstream from the transcription start site. The substituted residues in all but one of these FNR mutants are clustered around a presumed ...

journal_title：Molecular microbiology

authors： Bell A,Busby S

更新日期：1994-01-01 00:00:00

abstract：:TonB protein functions as an energy transducer, coupling cytoplasmic membrane electrochemical potential to the active transport of vitamin B12 and Fe(III)-siderophore complexes across the outer membrane of Escherichia coli and other Gram-negative bacteria. Accumulated evidence indicates that TonB is anchored in the cy...

journal_title：Molecular microbiology

authors： Larsen RA,Wood GE,Postle K

更新日期：1993-12-01 00:00:00