Abstract:
:The bacterial enhancer binding proteins (bEBP) are members of the AAA+ protein family and have a highly conserved 'DE' Walker B motif thought to be involved in the catalytic function of the protein with an active role in nucleotide hydrolysis. Based on detailed structural data, we analysed the functionality of the conserved 'DE' Walker B motif of a bEBP model, phage shock protein F (PspF), to investigate the role of these residues in the sigma(54)-dependent transcription activation process. We established their role in the regulation of PspF self-association and in the relay of the ATPase activity to the remodelling of an RNA polymerase.promoter complex (Esigma(54).DNA). Specific substitutions of the conserved glutamate (E) allowed the identification of new functional ATP.bEBP.Esigma(54) complexes which are stable and transcriptionally competent, providing a new tool to study the initial events of the sigma(54)-dependent transcription activation process. In addition, we show the importance of this glutamate residue in sigma(54).DNA conformation sensing, permitting the identification of new intermediate stages within the transcription activation pathway.
journal_name
Mol Microbioljournal_title
Molecular microbiologyauthors
Joly N,Rappas M,Wigneshweraraj SR,Zhang X,Buck Mdoi
10.1111/j.1365-2958.2007.05901.xsubject
Has Abstractpub_date
2007-11-01 00:00:00pages
583-95issue
3eissn
0950-382Xissn
1365-2958pii
MMI5901journal_volume
66pub_type
杂志文章abstract::The ferric uptake regulator (Fur) protein is known to act as a Fe2+-dependent transcriptional repressor of bacterial promoters. Here, we show that, in Helicobacter pylori, Fur can mediate the regulation of iron-activated genes in contrast to classical Fur regulation, in which iron acts as a co-repressor. Inactivation ...
journal_title:Molecular microbiology
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journal_title:Molecular microbiology
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pub_type: 杂志文章,评审
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journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:2017-03-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
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