Abstract:
:The protozoan parasite Trypanosoma brucei develops antigenic variation to escape the immune response of its host. To this end, the trypanosome genome contains multiple telomeric expression sites competent for transcription of variant surface glycoprotein genes, but as a rule only a single antigen is expressed at any time. We used reverse transcription-PCR (RT-PCR) to analyse transcription of different segments of the expression sites in different variant clones of two independent strains of T. brucei. The results indicated that RNA polymerase is installed and active at the beginning of many, if not all, expression sites simultaneously, but that a progressive arrest of RNA elongation occurs in all but one site. This defect is linked to inefficient RNA processing and RNA release from the nucleus. Therefore, functional transcription in the active site appears to depend on the selective recruitment of a RNA elongation/processing machinery.
journal_name
Mol Microbioljournal_title
Molecular microbiologyauthors
Vanhamme L,Poelvoorde P,Pays A,Tebabi P,Van Xong H,Pays Edoi
10.1046/j.1365-2958.2000.01844.xsubject
Has Abstractpub_date
2000-04-01 00:00:00pages
328-40issue
2eissn
0950-382Xissn
1365-2958pii
mmi1844journal_volume
36pub_type
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