Mapping of the msDNA operon in the chromosome of Escherichia coli B.

abstract：:A gene bank of Azorhizobium caulinodans DNA constructed in the bacteriophage lambda GEM11 was screened with Rhizobium meliloti fixL and fixJ genes as probes. One positive recombinant phage, ORS lambda L, was isolated. The nucleotide sequence of a 3.7 kb fragment was established. Two open reading frames of 1512bp and 6...

journal_title：Molecular microbiology

authors： Kaminski PA,Elmerich C

更新日期：1991-03-01 00:00:00

abstract：:The porin oprE gene of Pseudomonas aeruginosa PAO1 was isolated. Its nucleotide sequence indicated that the structural gene of 1383 nucleotide residues encodes a precursor consisting of 460 amino acid residues with a signal peptide of 29 amino acid residues, which was confirmed by the N-terminal 23-amino-acid sequence...

journal_title：Molecular microbiology

authors： Yamano Y,Nishikawa T,Komatsu Y

更新日期：1993-05-01 00:00:00

abstract：:A 2 kb DNA fragment isolated from a cosmid library of Aquaspirillum magnetotacticum strain MS-1 complements the aromatic-metabolite requirements and iron-uptake deficiencies of Escherichia coli and Salmonella typhimurium strains that lack a functional aroD (biosynthetic dehydrodquinase) sequence. All recombinant cosmi...

journal_title：Molecular microbiology

authors： Berson AE,Hudson DV,Waleh NS

更新日期：1991-09-01 00:00:00

abstract：:The Gram-negative bacterium Vibrio cholerae is the infectious agent responsible for the disease Asiatic cholera. The genes required for V. cholerae virulence, such as those encoding the cholera toxin (CT) and toxin-coregulated pilus (TCP), are controlled by a cascade of transcriptional activators. Ultimately, the dire...

journal_title：Molecular microbiology

authors： Withey JH,DiRita VJ

更新日期：2005-05-01 00:00:00

abstract：:IS231 constitutes a family of insertion sequences widespread among Bacillus thuringiensis subspecies. Three new IS231 variants have been isolated from B. thuringiensis subspecies finitimus (IS231 D and E) and israelensis (IS231F). Like the previously described IS231A, B and C, these 1.7 kb elements display single open...

journal_title：Molecular microbiology

authors： Rezsöhazy R,Hallet B,Delcour J

更新日期：1992-07-01 00:00:00

abstract：:The Escherichia coli UvrD helicase (or helicase II) is known for its involvement in DNA repair. We report that UvrD is required for DNA replication of several different rolling-circle plasmids in E. coli, whereas its homologue, the Rep helicase, is not. Lack of UvrD helicase does not impair the first step of plasmid r...

journal_title：Molecular microbiology

authors： Bruand C,Ehrlich SD

更新日期：2000-01-01 00:00:00

abstract：:The inv locus of Yersinia enterocolitica is sufficient to convert a non-invasive Escherichia coli K12 strain into a microorganism that is able to penetrate cultured mammalian cells. The nucleotide sequence of inv reveals an open reading frame corresponding to an 835-amino-acid protein that is homologous to the invasin...

journal_title：Molecular microbiology

authors： Young VB,Miller VL,Falkow S,Schoolnik GK

更新日期：1990-07-01 00:00:00

abstract：:Two distinct merR genes, which regulate expression of the mercuric ion resistance gene (mer), of Thiobacillus ferrooxidans strain E-15 have been cloned, sequenced and termed merR1 and merR2. As a result of gene walking around two merR genes, it was found that these two genes were quite close in distance. The nucleotid...

journal_title：Molecular microbiology

authors： Inoue C,Sugawara K,Kusano T

更新日期：1991-11-01 00:00:00

abstract：:The role of cAMP signalling during germination of asexual spores (conidia) of the filamentous fungus Aspergillus nidulans was investigated. A. nidulans strains defective for adenylate cyclase (CyaA) or for the functionally overlapping cAMP-dependent protein kinase (PkaA) and newly characterized SchA protein kinase, ho...

journal_title：Molecular microbiology

authors： Fillinger S,Chaveroche MK,Shimizu K,Keller N,d'Enfert C

更新日期：2002-05-01 00:00:00

abstract：:Small GTP-binding proteins of the highly conserved Rho family act as molecular switches regulating cell signalling, cytoskeletal organization and vesicle trafficking in eukaryotic cells. Here we show that in the dimorphic plant pathogenic fungus Ustilago maydis deletion of either cdc42 or rac1 results in loss of virul...

journal_title：Molecular microbiology

authors： Mahlert M,Leveleki L,Hlubek A,Sandrock B,Bölker M

更新日期：2006-01-01 00:00:00

abstract：:Legionella pneumophila, the causative agent of Legionnaires' disease, survives in macrophages by altering the endocytic pathway of its host cell. To accomplish this, the bacterium utilizes a type IVB secretion system to deliver effector molecules into the host cell cytoplasm. In a previous report, we performed an exte...

journal_title：Molecular microbiology

authors： Vincent CD,Friedman JR,Jeong KC,Sutherland MC,Vogel JP

更新日期：2012-07-01 00:00:00

abstract：:Rpc25 is a strongly conserved subunit of RNA polymerase III with homology to Rpa43 in RNA polymerase I, Rpb7 in RNA polymerase II and the archaeal RpoE subunit. A central domain of Rpc25 can replaced the corresponding region of Rpb7 with little or no growth defect, underscoring the functional relatedness of these prot...

journal_title：Molecular microbiology

authors： Zaros C,Thuriaux P

更新日期：2005-01-01 00:00:00

abstract：:Copper ions are essential but also very toxic. Copper resistance in bacteria is based on export of the toxic ion, oxidation from Cu(I) to Cu(II), and sequestration by copper-binding metal chaperones, which deliver copper ions to efflux systems or metal-binding sites of copper-requiring proteins. In their publication i...

journal_title：Molecular microbiology

authors： Nies DH,Herzberg M

更新日期：2013-02-01 00:00:00

abstract：:The type-VII ESX-1 secretion apparatus, encoded by the esx-1 genetic locus, is essential for the export of EsxA and EsxB, two major virulence factors of Mycobacterium tuberculosis. ESX-1 also requires the products of the unlinked espACD operon for optimal function and these proteins are considered integral parts of th...

journal_title：Molecular microbiology

authors： Chen JM,Zhang M,Rybniker J,Boy-Röttger S,Dhar N,Pojer F,Cole ST

更新日期：2013-09-01 00:00:00

abstract：:Recent footprinting, sedimentation and neutron-scattering results obtained in vivo or on pre-translocation and post-translocation ribosomal complexes are integrated with cross-linking and immunoelectron microscopy information. It is proposed that the 30S subunit pulses during translocation and that its pre- and post-t...

journal_title：Molecular microbiology

authors： Laughrea M

更新日期：1994-03-01 00:00:00

abstract：:The Rhizobium leguminosarum biovar phaseoli symbiotic plasmid pRP2JI carries a gene, melA, specifying the enzyme tyrosinase, which is responsible for the production of the pigment melanin in these bacteria. Transcription of melA is activated by the nifA gene of Rhizobium and, when the cloned melA gene is transferred t...

journal_title：Molecular microbiology

authors： Hawkins FK,Johnston AW

更新日期：1988-05-01 00:00:00

abstract：:A key component to the success of Mycobacterium tuberculosis as a pathogen is the ability to sense and adapt metabolically to the diverse range of conditions encountered in vivo, such as oxygen tension, environmental pH and nutrient availability. Although nitrogen is an essential nutrient for every organism, little is...

journal_title：Molecular microbiology

authors： Williams KJ,Jenkins VA,Barton GR,Bryant WA,Krishnan N,Robertson BD

更新日期：2015-09-01 00:00:00

abstract：:The haemolytic activity of Serratia marcescens is determined by two proteins, ShlA and ShlB. ShlA integrates into the erythrocyte membrane and causes osmotic lysis through channel formation. The conformation of ShlA and its interaction with erythrocyte membranes were studied by determining the cleavage of ShlA by adde...

journal_title：Molecular microbiology

authors： Schiebel E,Braun V

更新日期：1989-03-01 00:00:00

abstract：:The PII protein has been considered pivotal to the dual cascade regulating ammonia assimilation through glutamine synthetase activity. Here we show that PII, encoded by the glnB gene, is not always essential; for instance upon ammonia deprivation of a glnB deletion strain, glutamine synthetase can be deadenylylated as...

journal_title：Molecular microbiology

authors： van Heeswijk WC,Hoving S,Molenaar D,Stegeman B,Kahn D,Westerhoff HV

更新日期：1996-07-01 00:00:00

abstract：:Quinone-based compounds have been exploited to treat infectious diseases and cancer, with such chemicals often functioning as inhibitors of key metabolic pathways or as prodrugs. Here, we screened an aziridinyl 1,4-benzoquinone (ABQ) library against the causative agents of trypanosomiasis, and cutaneous leishmaniasis,...

journal_title：Molecular microbiology

authors： Meredith EL,Kumar A,Konno A,Szular J,Alsford S,Seifert K,Horn D,Wilkinson SR

更新日期：2017-10-01 00:00:00

abstract：:In Escherichia coli the flagellar regulon consists of more than 60 genes organized in three hierarchically and temporally regulated transcriptional classes. The flagellar sigma factor FliA (sigma(28)) is responsible for class 3 expression and, in the early phase of flagellar assembly, is inhibited by its anti-sigma fa...

journal_title：Molecular microbiology

authors： Barembruch C,Hengge R

更新日期：2007-07-01 00:00:00

abstract：:In Saccharomyces cerevisiae, the transcription factors Gln3p and Nil1p of the GATA family play a determinant role in expression of genes that are subject to nitrogen catabolite repression. Here we report the isolation of a new yeast mutant, gan1-1, exhibiting dramatically decreased NAD-linked glutamate dehydrogenase (...

journal_title：Molecular microbiology

authors： Soussi-Boudekou S,André B

更新日期：1999-02-01 00:00:00

abstract：:In Pseudomonas aeruginosa three type VI secretion systems (T6SSs) coexist, called H1- to H3-T6SSs. Several T6SS components are proposed to be part of a macromolecular complex resembling the bacteriophage tail. The T6SS protein HsiE1 (TagJ) is unique to the H1-T6SS and absent from the H2- and H3-T6SSs. We demonstrate t...

journal_title：Molecular microbiology

authors： Lossi NS,Manoli E,Simpson P,Jones C,Hui K,Dajani R,Coulthurst SJ,Freemont P,Filloux A

更新日期：2012-10-01 00:00:00

abstract：:The MtrCDE multidrug pump, from Neisseria gonorrhoeae, is assembled from the inner and outer membrane proteins MtrD and MtrE, which are connected by the periplasmic membrane fusion protein MtrC. Although it is clear that MtrD delivers drugs to the channel of MtrE, it remains unclear how drug delivery and channel openi...

journal_title：Molecular microbiology

authors： Janganan TK,Bavro VN,Zhang L,Borges-Walmsley MI,Walmsley AR

更新日期：2013-05-01 00:00:00

abstract：:The Escherichia coli dam gene and upstream sequences were cloned from the Kohara phage 4D4. Five promoters were found to contribute to dam gene transcription. P1 and P2 (the major promoter) were situated approximately 3.5 kb upstream of the structural gene, P3 was within the aroB gene, P4 was within the urf74.3 gene, ...

journal_title：Molecular microbiology

authors： Løbner-Olesen A,Boye E,Marinus MG

更新日期：1992-07-01 00:00:00

abstract：:Elaboration of a capsule composed of one of a range of acidic polysaccharides is a common feature of many bacteria, particularly those capable of causing serious infections in humans. Biochemical and genetical analyses of capsule biogenesis in Escherichia coli are beginning to reveal new aspects of polysaccharide bios...

journal_title：Molecular microbiology

authors： Boulnois GJ,Jann K

更新日期：1989-12-01 00:00:00

abstract：:Pseudomonas aeruginosa, an opportunistic human pathogen, is a major causative agent of mortality and morbidity in immunocompromised individuals and those with cystic fibrosis (CF). In CF patients, the secretion of abnormally high amounts of mucus into the airways contributes to their susceptibility to infection by P. ...

journal_title：Molecular microbiology

authors： Wang J,Lory S,Ramphal R,Jin S

更新日期：1996-12-01 00:00:00

abstract：:Filamentous heterocyst-forming cyanobacteria are a beautiful example of prokaryotic multicellularity. The filaments can achieve simultaneous nitrogen fixation and oxygenic photosynthesis by cooperation between two cell types: the photosynthetic vegetative cells and the nitrogen-fixing heterocysts. The multicellular fe...

journal_title：Molecular microbiology

authors： Mullineaux CW,Nürnberg DJ

更新日期：2014-12-01 00:00:00

abstract：:In bacteria, DNA supercoil movement is restricted to subchromosomal regions or 'domains.' To elucidate the nature of domain boundaries, we analysed reaction kinetics for gammadelta site-specific resolution in six chromosomal intervals ranging in size from 14 to 90 kb. In stationary cultures of Salmonella typhimurium, ...

journal_title：Molecular microbiology

authors： Staczek P,Higgins NP

更新日期：1998-09-01 00:00:00

abstract：:Clostridium difficile, an emerging nosocomial pathogen of increasing clinical significance, produces two large protein toxins that are responsible for the cellular damage associated with the disease. The precise mechanisms by which toxin synthesis is regulated in response to environmental change have yet to be discove...

journal_title：Molecular microbiology

authors： Matamouros S,England P,Dupuy B

更新日期：2007-06-01 00:00:00