Abstract:
:Defensins represent an evolutionarily conserved group of small peptides with potent antibacterial activities. We report here that extracellular proteinases secreted by the human pathogens Pseudomonas aeruginosa, Enterococcus faecalis and Streptococcus pyogenes release dermatan sulphate by degrading dermatan sulphate-containing proteoglycans, such as decorin. Dermatan sulphate was found to bind to neutrophil-derived alpha-defensin, and this binding completely neutralized its bactericidal activity. During infection, proteoglycan degradation and release of dermatan sulphate may therefore represent a previously unknown virulence mechanism, which could serve as a target for novel antibacterial strategies.
journal_name
Mol Microbioljournal_title
Molecular microbiologyauthors
Schmidtchen A,Frick IM,Björck Ldoi
10.1046/j.1365-2958.2001.02251.xsubject
Has Abstractpub_date
2001-02-01 00:00:00pages
708-13issue
3eissn
0950-382Xissn
1365-2958pii
mmi2251journal_volume
39pub_type
杂志文章abstract::Agrobacterium tumefaciens transforms plants by transferring DNA to the plant cell nucleus. The VirB membrane proteins are postulated to form a pore for the transport of the DNA across the bacterial membranes. Immunofluorescence and immunoelectron microscopy were used to study the transport pore complex. Three likely c...
journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2958.2000.01876.x
更新日期:2000-05-01 00:00:00
abstract::BldD generally functions as a repressor controlling morphological development of Streptomyces. In this work, evidences that BldD also activates antibiotic production are provided. In Streptomyces roseosporus (which produces daptomycin widely used for treatment of human infections), deletion of bldD notably reduced dap...
journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/mmi.14405
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abstract::Conjugative transfer of toxin and antibiotic resistance plasmids in Clostridium perfringens is mediated by the tcp conjugation locus. Surprisingly, neither a relaxase gene nor an origin of transfer (oriT) has been identified on these plasmids, which are typified by the 47 kb tetracycline resistance plasmid pCW3. The t...
journal_title:Molecular microbiology
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2958.1995.tb02279.x
更新日期:1995-03-01 00:00:00
abstract::Chlamydiae are bacterial parasites that carry out a distinct developmental cycle within host cells; however, the mechanisms by which these organisms regulate stage-specific gene expression are not known. We identified a DNA element located between nucleotide (nt) -135 and -90 upstream from the transcription start poin...
journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2958.2001.02531.x
更新日期:2001-07-01 00:00:00
abstract::The de novo purine biosynthetic enzymes 5-amino-4-imidazolecarboxamide-ribonucleotide (AICAR) transformylase (EC 2.1.2.3), IMP cyclohydrolase (EC 3.5.4.10) and glycineamide-ribonucleotide (GAR) synthetase (EC 2.1.2.2) are encoded by the purHD locus of Escherichia coli. The DNA sequence of this locus revealed two open ...
journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2958.1990.tb00605.x
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abstract::The flexible pilus of Aeromonas hydrophila is a morphologically and biochemically unique organelle which binds eukaryotic cell surfaces and whose expression is induced by specific physiochemical conditions. fxp, the structural gene coding for the flexible pilus subunit, was localized on a 7.6kb plasmid of A. hydrophil...
journal_title:Molecular microbiology
pub_type: 杂志文章
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abstract::The mechanisms by which micro-organisms sense and internalize extracellular pH signals are not completely understood. One example of a known external pH-sensing process is the fungal-specific Rim/Pal signal transduction pathway. Fungi, such as the opportunistic pathogen Cryptococcus neoformans, use Rim signaling to se...
journal_title:Molecular microbiology
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doi:10.1111/mmi.13998
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pub_type: 杂志文章
doi:10.1111/mmi.12749
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abstract::The majority of characterized bacterial dipeptide permeases (Dpp) are membrane-associated complexes of five proteins belonging to the ABC-transporter family. They have been found to be involved in the uptake of essential amino acids, haem production, chemotaxis and sporulation. A 5.8 kb genomic DNA fragment of the ser...
journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2958.1998.00898.x
更新日期:1998-06-01 00:00:00
abstract::To identify Rhodobacter capsulatus nif genes necessary for the alternative nitrogenase, strains carrying defined mutations in 32 genes and open reading frames of nif region A, B or C were constructed. The ability of these mutants to grow on nitrogen-free medium with molybdenum (Nif phenotype) or in a nifHDK deletion b...
journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:1993-05-01 00:00:00
abstract::A recurring theme in morphogenesis is the coupling of the expression of genes that drive morphogenesis and the morphogenetic process per se. This coupling ensures that gene expression and morphogenesis are carried out in synchrony. Morphogenesis of the spore-filled fruiting bodies in Myxococcus xanthus illustrates thi...
journal_title:Molecular microbiology
pub_type: 杂志文章,评审
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更新日期:2003-04-01 00:00:00
abstract::Haemophilus influenzae is a human-specific pathogen and a major source of morbidity worldwide. Infection with this organism begins with colonization of the nasopharynx, a process that probably depends on adherence to respiratory epithelium. The Hia autotransporter protein is the major adhesin ex-pressed by a subset of...
journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2958.2002.03189.x
更新日期:2002-11-01 00:00:00
abstract::While Vipp1 (also known as IM30) clearly is essential for proper biogenesis of thylakoid membranes in chloroplasts and cyanobacteria, the exact function of Vipp1/IM30 still remains unclear. The recent in vivo study of Gutu et al. now demonstrates that Vipp1/IM30 forms localized puncta specifically at highly curved mem...
journal_title:Molecular microbiology
pub_type: 评论,杂志文章
doi:10.1111/mmi.13924
更新日期:2018-04-01 00:00:00
abstract::Early diverged extant organisms, which may serve as convenient laboratory models to look for and study evolutionary ancient features of eukaryotic cell biology, are rare. The diplomonad Giardia intestinalis, a protozoan parasite known to cause diarrhoeal disease, has become an increasingly popular object of basic rese...
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更新日期:2004-07-01 00:00:00
abstract::In Xanthomonas campestris pv. phaseoli, a gene for the alkyl hydroperoxide reductase subunit C (ahpC) had unique patterns of regulation by various forms of OxyR. Reduced OxyR repressed expression of the gene, whereas oxidized OxyR activated its expression. This dual regulation of ahpC is unique and unlike all other Ox...
journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2958.2000.02107.x
更新日期:2000-09-01 00:00:00
abstract::Legionella pneumophila, the causative agent of Legionnaires' disease, survives in macrophages by altering the endocytic pathway of its host cell. To accomplish this, the bacterium utilizes a type IVB secretion system to deliver effector molecules into the host cell cytoplasm. In a previous report, we performed an exte...
journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2958.2012.08118.x
更新日期:2012-07-01 00:00:00
abstract::The nucleotide sequence of the ugp genes of Escherichia coli K-12, which encode a phosphate-limitation inducible uptake system for sn-glycerol-3-phosphate and glycerophosphoryl diesters, was determined. The genetic organization of the operon differed from previously published results. A single promoter, containing a p...
journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:1988-11-01 00:00:00
abstract::The Helicobacter pylori vacuolating cytotoxin (VacA) induces degenerative vacuolation of sensitive mammalian cell lines. Although evidence is accumulating that VacA enters cells and functions from an intracellular site of action, the biochemical mechanism by which VacA mediates cellular vacuolation has not been establ...
journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2958.2002.02818.x
更新日期:2002-03-01 00:00:00
abstract::The process of bacterial cell division involves the assembly of a complex of proteins at the site of septation that probably provides both the structural and the cytokinetic functions required for elaboration and closure of the septal annulus. During sporulation in Bacillus subtilis, this complex of proteins is modifi...
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pub_type: 杂志文章
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journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:2011-05-01 00:00:00
abstract::Alternative sigma (σ) factors govern expression of bacterial genes in response to diverse environmental signals. In Pseudomonas aeruginosa σPvdS directs expression of genes for production of a siderophore, pyoverdine, as well as a toxin and a protease. σFpvI directs expression of a receptor for ferripyoverdine import....
journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/mmi.13855
更新日期:2017-12-01 00:00:00
abstract::The sensor kinase/response regulator system KdpD/KdpE of Escherichia coli regulates the expression of the kdpFABC operon, which encodes the high affinity K+ transport system KdpFABC. The membrane-bound sensor kinase KdpD consists of four transmembrane domains, a large cytoplasmic N-terminal domain and a cytoplasmic C-...
journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2958.2003.03348.x
更新日期:2003-02-01 00:00:00
abstract::Electron microscopic studies have demonstrated that various gliding filamentous cyanobacteria have trichome surfaces with a common structural organization. They contain an S-layer attached to the outer membrane and an array of parallel fibrils on top of the S-layer. In all species studied, the helical arrangement of t...
journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2958.1997.5971972.x
更新日期:1997-11-01 00:00:00
abstract::The newly sequenced genome of Streptomyces coelicolor is estimated to encode 7825 theoretical proteins. We have mapped approximately 10% of the theoretical proteome experimentally using two-dimensional gel electrophoresis and matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry. Pro...
journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2958.2002.03219.x
更新日期:2002-11-01 00:00:00
abstract::Chromosomes are divided into topologically independent regions, called domains, by the action of uncharacterized barriers. With the goal of identifying domain barrier components, we designed a genetic selection for mutants with reduced negative supercoiling of the Escherichia coli chromosome. We employed a strain that...
journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2958.2005.04799.x
更新日期:2005-09-01 00:00:00
abstract::The GATA factor AreA is a wide-domain regulator in Aspergillus nidulans with transcriptional activation and chromatin remodelling functions. AreA interacts with the nitrate-specific Zn(2)-C(6) cluster protein NirA and both proteins cooperate to synergistically activate nitrate-responsive genes. We have previously esta...
journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2958.2005.04957.x
更新日期:2006-01-01 00:00:00
abstract::The effect of the unc transcription terminator on expression of uncC, encoding the epsilon subunit of Escherichia coli F1-ATPase, from plasmids was studied. The cloned sequence in pTK1 included the uncC ribosome binding site, the uncC structural gene, and the unc transcription terminator. The cloned region in pSD37 wa...
journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2958.1990.tb02043.x
更新日期:1990-11-01 00:00:00
abstract::A new mechanism for the turning-off of gene expression in Salmonella Pathogenicity Island 1 (SPI-1) is proposed by Espinosa and Casadesús, which involves the action of the LeuO quiescent regulator, by two different pathways. A major one through the activation of the hilE gene, where the HilE protein would in turn inac...
journal_title:Molecular microbiology
pub_type: 评论,杂志文章
doi:10.1111/mmi.12514
更新日期:2014-03-01 00:00:00
abstract::Among players in neurobiology, ion channels are the demigods that underlie all our senses, behaviour and intelligence. In animals, these 'gated pores' detect ligands, voltage, heat or stretch forces and emit electric or ionic signals. Patch clamp and genome sequencing now show that nearly all microbes also have these ...
journal_title:Molecular microbiology
pub_type: 杂志文章,评审
doi:10.1111/j.1365-2958.2004.04180.x
更新日期:2004-07-01 00:00:00