Abstract:
:A transposon mutant of Escherichia coli 5K was isolated which reduced 10- to 50-fold the secreted extracellular haemolytic activity of cells carrying the complete hlyCABD operon while leaving unaffected the intracellular haemolytic activity and the levels of intracellular and extracellular haemolysin protein, HlyA. The transposon insertion was identified within the rfaP gene (required for attachment of phosphate-containing substituents to the lipopolysaccharide inner core), and extracellular haemolytic activity was restored in trans by the intact rfaP gene. The loss in cytolytic activity of the secreted HlyA protein was not related to the HlyC-directed acylation of the protoxin. Activity of the secreted toxin was restored by chaotropic agents and during rate-zonal centrifugation the mutant-secreted HlyA migrated as a larger species than the wild type. The results indicate that the rfaP mutation affects the aggregation behaviour of the active toxin during or following the signal peptide-independent secretion process.
journal_name
Mol Microbioljournal_title
Molecular microbiologyauthors
Stanley PL,Diaz P,Bailey MJ,Gygi D,Juarez A,Hughes Cdoi
10.1111/j.1365-2958.1993.tb00948.xsubject
Has Abstractpub_date
1993-11-01 00:00:00pages
781-7issue
4eissn
0950-382Xissn
1365-2958journal_volume
10pub_type
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