Screening Leishmania donovani-specific genes required for visceral infection.

abstract：:In response to discrete environmental cues, Saccharomyces cerevisiae cells adjust patterns of gene expression and protein activity to optimize metabolism. Nutrient-sensing systems situated in the plasma membrane (PM) of yeast have only recently been discovered. Ssy1p is one of three identified components of the Ssy1p-...

journal_title：Molecular microbiology

authors： Forsberg H,Gilstring CF,Zargari A,Martínez P,Ljungdahl PO

更新日期：2001-10-01 00:00:00

abstract：:Bacteria of Shigella spp. are responsible for shigellosis in humans. They use a type III secretion (TTS) system encoded by a 200 kb virulence plasmid to enter epithelial cells and trigger apoptosis in macrophages. This TTS system comprises a secretion apparatus, translocators and effectors that transit through this ap...

journal_title：Molecular microbiology

authors： Penno C,Sansonetti P,Parsot C

更新日期：2005-04-01 00:00:00

abstract：:Saccharomyces cerevisiae neutral trehalase, encoded by NTH1, controls trehalose hydrolysis in response to multiple stress conditions, including nutrient limitation. The presence of three stress responsive elements (STREs, CCCCT) in the NTH1 promoter suggested that the transcriptional activator proteins Msn2 and Msn4, ...

journal_title：Molecular microbiology

authors： Zähringer H,Thevelein JM,Nwaka S

更新日期：2000-01-01 00:00:00

abstract：:We report the cloning and determination of the nucleotide sequence of the gene encoding nucleoside diphosphate kinase (Ndk) from Pseudomonas aeruginosa. The amino acid sequence of Ndk was highly homologous with other known bacterial and eukaryotic Ndks (39.9 to 58.3% amino acid identity). We have previously reported t...

journal_title：Molecular microbiology

authors： Sundin GW,Shankar S,Chugani SA,Chopade BA,Kavanaugh-Black A,Chakrabarty AM

更新日期：1996-06-01 00:00:00

abstract：:The majority of bacterial genomes encode a high number of two-component systems controlling gene expression in response to a variety of different stimuli. The Gram-positive soil bacterium Corynebacterium glutamicum contains two homologous two-component systems (TCS) involved in the haem-dependent regulation of gene ex...

journal_title：Molecular microbiology

authors： Hentschel E,Mack C,Gätgens C,Bott M,Brocker M,Frunzke J

更新日期：2014-06-01 00:00:00

abstract：:Beta-oxidation (beta-ox) occurs exclusively in the peroxisomes of Saccharomyces cerevisiae and other yeasts, leading to the supposition that fungi lack mitochondrial beta-ox. Here we present unequivocal evidence that the filamentous fungus Aspergillus nidulans houses both peroxisomal and mitochondrial beta-ox. While g...

journal_title：Molecular microbiology

authors： Maggio-Hall LA,Keller NP

更新日期：2004-12-01 00:00:00

abstract：:Cyclic di-GMP (c-di-GMP) controls the transition between sessility and motility in many bacterial species. This regulation is achieved by a variety of mechanisms including alteration of transcription initiation and inhibition of flagellar function. How c-di-GMP inhibits the motility of Vibrio cholerae has not been det...

journal_title：Molecular microbiology

authors： Srivastava D,Hsieh ML,Khataokar A,Neiditch MB,Waters CM

更新日期：2013-12-01 00:00:00

abstract：:Five amino acids (Y105, Y176, Y189, Y189, W207) that constitute the substrate binding site of PHB depolymerase PhaZ7 were identified. All residues are located at a single surface-exposed location of PhaZ7. Exchange of these amino acids by less hydrophobic, hydrophilic or negatively charged residues reduced binding of ...

journal_title：Molecular microbiology

authors： Jendrossek D,Hermawan S,Subedi B,Papageorgiou AC

更新日期：2013-11-01 00:00:00

abstract：:Agrobacterium tumefaciens is a plant pathogen that persists as surface-associated populations on plants or soil particles. A genetic screen for A. tumefaciens mutants deficient for surface interactions identified a mutant that forms thin, sparsely populated biofilms, but is proficient for initial attachment. The mutan...

journal_title：Molecular microbiology

authors： Ramey BE,Matthysse AG,Fuqua C

更新日期：2004-06-01 00:00:00

abstract：:Photorhabdus and Xenorhabdus bacteria colonize the intestines of the infective soil-dwelling stage of entomophagous nematodes, Heterorhabditis and Steinernema, respectively. These nematodes infect susceptible insect larvae and release the bacteria into the insect blood. The bacteria kill the insect larvae and convert ...

journal_title：Molecular microbiology

authors： Goodrich-Blair H,Clarke DJ

更新日期：2007-04-01 00:00:00

abstract：:In this study we uncover two genes in Lactobacillus brevis ATCC 367, tstT and tstR, encoding for a rhodanese and a transcriptional regulator involved in cyanide detoxification. TstT (LVIS_0852) belongs to a new class of thiosulphate:cyanide sulphurtransferases. We found that TstR (LVIS_0853) modulates both the express...

journal_title：Molecular microbiology

authors： Pagliai FA,Murdoch CC,Brown SM,Gonzalez CF,Lorca GL

更新日期：2014-05-01 00:00:00

abstract：:We studied the molecular mechanism of neuronal calcium sensor-1 (NCS-1) signaling pathway for tolerance to Ca2+ stress in Neurospora crassa. Increasing concentration of Ca2+ increased the expression of ncs-1; however, the calcineurin inhibitor FK506 severely reduced ncs-1 mRNA transcript levels. Chromatin immunoprecip...

journal_title：Molecular microbiology

authors： Gohain D,Tamuli R

更新日期：2019-06-01 00:00:00

abstract：:Wolinella succinogenes can grow by anaerobic respiration with nitrate or nitrite using formate as electron donor. Two forms of nitrite reductase were isolated from the membrane fraction of W. succinogenes. One form consisted of a 58 kDa polypeptide (NrfA) that was identical to the periplasmic nitrite reductase. The ot...

journal_title：Molecular microbiology

authors： Simon J,Gross R,Einsle O,Kroneck PM,Kröger A,Klimmek O

更新日期：2000-02-01 00:00:00

abstract：:Amino acid sequence analyses have indicated that the amino-terminal part of bacteriophage PRD1 structural protein P7 carries a conserved transglycosylase domain. We analysed wild-type PRD1 and different mutant particles in zymograms and found a glycolytic activity that was associated with protein P7. This is the first...

journal_title：Molecular microbiology

authors： Rydman PS,Bamford DH

更新日期：2000-07-01 00:00:00

abstract：:DNA double-strand break repair can be accomplished by homologous recombination when a sister chromatid or a homologous chromosome is available. However, the study of sister chromatid double-strand break repair in prokaryotes is complicated by the difficulty in targeting a break to only one copy of two essentially iden...

journal_title：Molecular microbiology

authors： Cromie GA,Leach DR

更新日期：2001-08-01 00:00:00

abstract：:Release factors RF1 and RF2 are required in bacteria for the cleavage of peptidyl-tRNA. A single sequence motif, GGQ, is conserved in all eubacterial, archaebacterial and eukaryotic release factors and may mimic the CCA end of tRNA, although the position of the motif in the crystal structures of human eRF1 and Escheri...

journal_title：Molecular microbiology

authors： Mora L,Heurgué-Hamard V,Champ S,Ehrenberg M,Kisselev LL,Buckingham RH

更新日期：2003-01-01 00:00:00

abstract：:The bacteriocin pediocin PA-1 operon of Pediococcus acidilactici PAC1.0 encompasses four genes: pedA, pedB, pedC and pedD. Transcription of the operon results in the formation of two overlapping transcripts, probably originating from a single promoter upstream of pedA. The major transcript comprises pedA, pedB, and pe...

journal_title：Molecular microbiology

authors： Venema K,Kok J,Marugg JD,Toonen MY,Ledeboer AM,Venema G,Chikindas ML

更新日期：1995-08-01 00:00:00

abstract：:Successful pathogens must be able to swiftly respond to and repair DNA damages inflicted by the host defence. The replication protein A (RPA) complex plays multiple roles in DNA damage response and is regulated by phosphorylation. However, the regulators of RPA phosphorylation remain unclear. Here, we investigated Rfa...

journal_title：Molecular microbiology

authors： Gao J,Wang H,Wong AH,Zeng G,Huang Z,Wang Y,Sang J,Wang Y

更新日期：2014-10-01 00:00:00

abstract：:Francisella tularensis are the causative agent of the zoonotic disease, tularaemia. Among four F. tularensis subspecies, ssp. novicida (F. novicida) is pathogenic only for immunocompromised individuals, while all four subspecies are pathogenic for mice. This study utilized proteomic and bioinformatic approaches to ide...

journal_title：Molecular microbiology

authors： Hager AJ,Bolton DL,Pelletier MR,Brittnacher MJ,Gallagher LA,Kaul R,Skerrett SJ,Miller SI,Guina T

更新日期：2006-10-01 00:00:00

abstract：:We have previously described HpuAB, a two-component receptor that mediates binding to haemoglobin (Hb), haemoglobin-haptoglobin (Hb-Hp) and apo-haptoglobin (Hp). In this communication, we constructed non-polar mutations in the hpuA and hpuB loci to examine the individual roles of HpuA and HpuB. Our results indicate th...

journal_title：Molecular microbiology

authors： Lewis LA,Gipson M,Hartman K,Ownbey T,Vaughn J,Dyer DW

更新日期：1999-06-01 00:00:00

abstract：:The ctaD gene encoding subunit I of the aa3-type cytochrome c oxidase from Rhodobacter sphaeroides has been cloned. The gene encodes a polypeptide of 565 residues which is highly homologous to the sequences of subunit I from other prokaryotic and eukaryotic sources, e.g. 51% identity with that from bovine, and 75% ide...

journal_title：Molecular microbiology

authors： Shapleigh JP,Gennis RB

更新日期：1992-03-01 00:00:00

abstract：:The nucleotide sequence (6138 bp) of a microaerobically inducible region (hupV/VI) from the Rhizobium leguminosarum bv. viciae hydrogenase gene cluster has been determined. Six genes, arranged as a single operon, were identified, and designated hypA, B, F, C, D and E based on the sequence similarities of all of them, ...

journal_title：Molecular microbiology

authors： Rey L,Murillo J,Hernando Y,Hidalgo E,Cabrera E,Imperial J,Ruiz-Argüeso T

更新日期：1993-05-01 00:00:00

abstract：:The DNA encoding the zinc metalloprotease of Legionella pneumophila Philadelphia 1 has been isolated and expressed in Escherichia coli. This protein, which is 38,000 Daltons in size, possesses immunological and biochemical properties identical to those previously described for the purified L. pneumophila protease. Per...

journal_title：Molecular microbiology

authors： Quinn FD,Tompkins LS

更新日期：1989-06-01 00:00:00

abstract：:In vivo and in vitro analyses indicate that transcription of the argO gene coding for an arginine exporter is regulated by the global transcriptional regulator Lrp, an effect that went by unnoticed in previous genome-scale screenings of the Lrp regulatory network in Escherichia coli. Lrp activates the argO promoter fo...

journal_title：Molecular microbiology

authors： Peeters E,Nguyen Le Minh P,Foulquié-Moreno M,Charlier D

更新日期：2009-12-01 00:00:00

abstract：:Chlamydiae are bacterial parasites that carry out a distinct developmental cycle within host cells; however, the mechanisms by which these organisms regulate stage-specific gene expression are not known. We identified a DNA element located between nucleotide (nt) -135 and -90 upstream from the transcription start poin...

journal_title：Molecular microbiology

authors： Zhong J,Douglas AL,Hatch TP

更新日期：2001-07-01 00:00:00

abstract：:Helicobacter pylori colonization of the human stomach is characterized by profound disease-causing inflammation. Bacterial proteins that detoxify reactive oxygen species or recognize damaged DNA adducts promote infection, suggesting that H. pylori requires DNA damage repair for successful in vivo colonization. The mol...

journal_title：Molecular microbiology

authors： Amundsen SK,Fero J,Hansen LM,Cromie GA,Solnick JV,Smith GR,Salama NR

更新日期：2008-08-01 00:00:00

abstract：:The Incl1 conjugative plasmid Collb-P9 carries a psiB gene that prevents induction of the SOS response in host bacteria. This locus is located 2.5 kb downstream of the ssb (single-stranded DNA-binding protein) gene in the leading region. This portion of Collb is strikingly similar to part of the leading region of the ...

journal_title：Molecular microbiology

authors： Jones AL,Barth PT,Wilkins BM

更新日期：1992-03-01 00:00:00

abstract：:Helicobacter pylori is anomalous among non nitrogen-fixing bacteria in containing an incomplete NIF system for Fe-S cluster assembly comprising two essential proteins, NifS (cysteine desulfurase) and NifU (scaffold protein). Although nifU deletion strains cannot be obtained via the conventional gene replacement, a Nif...

journal_title：Molecular microbiology

authors： Benoit SL,Holland AA,Johnson MK,Maier RJ

更新日期：2018-05-01 00:00:00

abstract：:The identification, isolation and characterization of a new Aspergillus nidulans positive-acting gene metR, which encodes a transcriptional activator of sulphur metabolism, is reported. metR mutants are tight auxotrophs requiring methionine or homocysteine for growth. Mutations in the metR gene are epistatic to mutati...

journal_title：Molecular microbiology

authors： Natorff R,Sieńko M,Brzywczy J,Paszewski A

更新日期：2003-08-01 00:00:00

abstract：:The crystal structures of two active forms of dissimilatory sulphite reductase (Dsr) from Desulfovibrio gigas, Dsr-I and Dsr-II, are compared at 1.76 and 2.05 Å resolution respectively. The dimeric α2β2γ2 structure of Dsr-I contains eight [4Fe-4S] clusters, two saddle-shaped sirohaems and two flat sirohydrochlorins. I...

journal_title：Molecular microbiology

authors： Hsieh YC,Liu MY,Wang VC,Chiang YL,Liu EH,Wu WG,Chan SI,Chen CJ

更新日期：2010-12-01 00:00:00