Abstract:
:The ctaD gene encoding subunit I of the aa3-type cytochrome c oxidase from Rhodobacter sphaeroides has been cloned. The gene encodes a polypeptide of 565 residues which is highly homologous to the sequences of subunit I from other prokaryotic and eukaryotic sources, e.g. 51% identity with that from bovine, and 75% identity with that from Paracoccus denitrificans. The ctaD gene was deleted from the chromosome of R. sphaeroides, resulting in a strain that spectroscopically lacks cytochrome a. This strain maintains about 50% of the cytochrome c oxidase activity of the wild-type strain owing to the presence of an alternate o-type cytochrome c oxidase. The aa3-type oxidase was restored by complementing the chromosomal deletion with a plasmid-borne copy of the ctaD gene. This system is well suited for site-directed mutagenesis probing of the structure and function of cytochrome c oxidase.
journal_name
Mol Microbioljournal_title
Molecular microbiologyauthors
Shapleigh JP,Gennis RBdoi
10.1111/j.1365-2958.1992.tb01511.xsubject
Has Abstractpub_date
1992-03-01 00:00:00pages
635-42issue
5eissn
0950-382Xissn
1365-2958journal_volume
6pub_type
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