Abstract:
:Wild strains of Bacillus subtilis are capable of forming architecturally complex communities of cells known as biofilms. Critical to biofilm formation is the eps operon, which is believed to be responsible for the biosynthesis of an exopolysaccharide that binds chains of cells together in bundles. We report that transcription of eps is under the negative regulation of SinR, a repressor that was found to bind to multiple sites in the regulatory region of the operon. Mutations in sinR bypassed the requirement in biofilm formation of two genes of unknown function, ylbF and ymcA, and sinI, which is known to encode an antagonist of SinR. We propose that these genes are members of a pathway that is responsible for counteracting SinR-mediated repression. We further propose that SinR is a master regulator that governs the transition between a planktonic state in which the bacteria swim as single cells in liquid or swarm in small groups over surfaces, and a sessile state in which the bacteria adhere to each other to form bundled chains and assemble into multicellular communities.
journal_name
Mol Microbioljournal_title
Molecular microbiologyauthors
Kearns DB,Chu F,Branda SS,Kolter R,Losick Rdoi
10.1111/j.1365-2958.2004.04440.xsubject
Has Abstractpub_date
2005-02-01 00:00:00pages
739-49issue
3eissn
0950-382Xissn
1365-2958pii
MMI4440journal_volume
55pub_type
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