Heme catabolism in the causative agent of anthrax.


:A challenge common to all bacterial pathogens is to acquire nutrients from hostile host environments. Iron is an important cofactor required for essential cellular processes such as DNA repair, energy production and redox balance. Within a mammalian host, most iron is sequestered within heme, which in turn is predominantly bound by hemoglobin. While little is understood about the mechanisms by which bacterial hemophores attain heme from host-hemoglobin, even less is known about intracellular heme processing. Bacillus anthracis, the causative agent of anthrax, displays a remarkable ability to grow in mammalian hosts. Hypothesizing this pathogen harbors robust ways to catabolize heme, we characterize two new intracellular heme-binding proteins that are distinct from the previously described IsdG heme monooxygenase. The first of these, HmoA, binds and degrades heme, is necessary for heme detoxification and facilitates growth on heme iron sources. The second protein, HmoB, binds and degrades heme too, but is not necessary for heme utilization or virulence. The loss of both HmoA and IsdG renders B. anthracis incapable of causing anthrax disease. The additional loss of HmoB in this background increases clearance of bacilli in lungs, which is consistent with this protein being important for survival in alveolar macrophages.


Mol Microbiol


Molecular microbiology


Clark J,Terwilliger A,Nguyen C,Green S,Nobles C,Maresso A




Has Abstract


2019-08-01 00:00:00












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    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Morgan SJ,Felek S,Gadwal S,Koropatkin NM,Perry JW,Bryson AB,Krukonis ES

    更新日期:2011-07-01 00:00:00

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    abstract::In prokaryotes, the principal signal transduction systems operating at the level of protein phosphorylation are the two-component systems. A number of hybrid histidine protein kinases in these systems contain several receiver domains, however, the function of these receiver domains is unknown. The RodK kinase in Myxoc...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Rasmussen AA,Wegener-Feldbrügge S,Porter SL,Armitage JP,Søgaard-Andersen L

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    abstract::Dynamic oscillation of the Min system in Escherichia coli determines the placement of the division plane at the midcell. In addition to stimulating MinD ATPase activity, we report here that MinE can directly interact with the membrane and this interaction contributes to the proper MinDE localization and dynamics. The ...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Hsieh CW,Lin TY,Lai HM,Lin CC,Hsieh TS,Shih YL

    更新日期:2010-01-01 00:00:00

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    abstract::Expression of the Saccharomyces cerevisiae nuclear gene CYB2 encoding the mitochondrial enzyme L-(+)-lactate-cytochrome c oxidoreductase (EC is subject to several strict metabolic controls at the transcriptional level: repression due to glucose fermentation, derepression by ethanol, induction by lactate and i...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Ramil E,Agrimonti C,Shechter E,Gervais M,Guiard B

    更新日期:2000-09-01 00:00:00

  • The Aspergillus nidulans metR gene encodes a bZIP protein which activates transcription of sulphur metabolism genes.

    abstract::The identification, isolation and characterization of a new Aspergillus nidulans positive-acting gene metR, which encodes a transcriptional activator of sulphur metabolism, is reported. metR mutants are tight auxotrophs requiring methionine or homocysteine for growth. Mutations in the metR gene are epistatic to mutati...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


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  • Cloning, characterization and disruption of a (p)ppGpp synthetase gene (relA) of Streptomyces coelicolor A3(2).

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    pub_type: 杂志文章


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    journal_title:Molecular microbiology

    pub_type: 杂志文章


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    更新日期:2009-01-01 00:00:00

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    journal_title:Molecular microbiology

    pub_type: 杂志文章,评审


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    journal_title:Molecular microbiology

    pub_type: 杂志文章


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    更新日期:2010-06-01 00:00:00

  • Role of His residues in Bacillus subtilis cytochrome b558 for haem binding and assembly of succinate: quinone oxidoreductase (complex II).

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    journal_title:Molecular microbiology

    pub_type: 杂志文章


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    journal_title:Molecular microbiology

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  • Magnaporthe oryzae nucleoside diphosphate kinase is required for metabolic homeostasis and redox-mediated host innate immunity suppression.

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    pub_type: 杂志文章


    authors: Rocha RO,Wilson RA

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    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: da Silva-Tatley FM,Steyn LM

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    journal_title:Molecular microbiology

    pub_type: 杂志文章


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    更新日期:2003-01-01 00:00:00

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    abstract::Type IV secretion systems (T4SS) are utilized by a wide range of Gram negative bacteria to deliver protein and DNA substrates to recipient cells. The best characterized T4SS are the type IVA systems, which exhibit extensive similarity to the Agrobacterium VirB T4SS. In contrast, type IVB secretion systems share almost...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Vincent CD,Friedman JR,Jeong KC,Buford EC,Miller JL,Vogel JP

    更新日期:2006-12-01 00:00:00

  • Competitive activation of the Escherichia coli argO gene coding for an arginine exporter by the transcriptional regulators Lrp and ArgP.

    abstract::In vivo and in vitro analyses indicate that transcription of the argO gene coding for an arginine exporter is regulated by the global transcriptional regulator Lrp, an effect that went by unnoticed in previous genome-scale screenings of the Lrp regulatory network in Escherichia coli. Lrp activates the argO promoter fo...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Peeters E,Nguyen Le Minh P,Foulquié-Moreno M,Charlier D

    更新日期:2009-12-01 00:00:00

  • Tn5 transposase loops DNA in the absence of Tn5 transposon end sequences.

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    pub_type: 杂志文章


    authors: Adams CD,Schnurr B,Skoko D,Marko JF,Reznikoff WS

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    journal_title:Molecular microbiology

    pub_type: 杂志文章,评审


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    更新日期:2007-04-01 00:00:00

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    journal_title:Molecular microbiology

    pub_type: 杂志文章


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    abstract::Escherichia coli RnlA-RnlB is a newly identified toxin-antitoxin (TA) system that plays a role in bacteriophage resistance. RnlA functions as a toxin with mRNA endoribonuclease activity and the cognate antitoxin RnlB inhibits RnlA toxicity in E. coli cells. Interestingly, T4 phage encodes the antitoxin Dmd, which acts...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Wei Y,Gao ZQ,Otsuka Y,Naka K,Yonesaki T,Zhang H,Dong YH

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  • Purification and functional studies of a potent modified quorum-sensing peptide and a two-peptide bacteriocin in Streptococcus mutans.

    abstract::Bacteria use quorum-sensing signals or autoinducers to communicate. The signals in Gram-positive bacteria are often peptides activated by proteolytic removal of an N-terminal leader sequence. While investigating stimulation of antimicrobial peptide production by the Streptococcus mutans synthetic competence stimulatin...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Petersen FC,Fimland G,Scheie AA

    更新日期:2006-09-01 00:00:00

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    journal_title:Molecular microbiology

    pub_type: 杂志文章


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    更新日期:1999-08-01 00:00:00

  • The purified Shigella IpaB and Salmonella SipB translocators share biochemical properties and membrane topology.

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    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Hume PJ,McGhie EJ,Hayward RD,Koronakis V

    更新日期:2003-07-01 00:00:00

  • Evidence for the genetic interaction between the actin-binding protein Vrp1 and the RhoGAP Rgd1 mediated through Rho3p and Rho4p in Saccharomyces cerevisiae.

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    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Roumanie O,Peypouquet MF,Bonneu M,Thoraval D,Doignon F,Crouzet M

    更新日期:2000-06-01 00:00:00

  • Characterization of the diphtheria toxin receptor-binding domain.

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    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Rolf JM,Eidels L

    更新日期:1993-02-01 00:00:00

  • The sugar-specific outer membrane channel ScrY contains functional characteristics of general diffusion pores and substrate-specific porins.

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    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Schülein K,Schmid K,Benzl R

    更新日期:1991-09-01 00:00:00

  • The penicillin sensory transducer, BlaR, involved in the inducibility of beta-lactamase synthesis in Bacillus licheniformis is embedded in the plasma membrane via a four-alpha-helix bundle.

    abstract::Prediction studies, conformational analyses and membrane-topology mapping lead to the conclusion that the penicillin sensory transducer, BlaR, involved in the inducibility of beta-lactamase synthesis in Bacillus licheniformis, is embedded in the plasma membrane bilayer via four transmembrane segments TM1-TM4 that form...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Hardt K,Joris B,Lepage S,Brasseur R,Lampen JO,Frère JM,Fink AL,Ghuysen JM

    更新日期:1997-03-01 00:00:00