Abstract:
:The AMA1 sequence is an efficient plasmid replicator and transformation enhancer in Aspergillus nidulans. It comprises two long perfect inverted repeats (MATE elements) flanking a short, unique, central spacer. Subclone analysis indicates that the complete inverted duplication, but not the unique central spacer, is necessary for efficient plasmid replication. The smallest fragments able to affect transformation efficiency lie within the AT-rich portions of the inverted repeats. We demonstrate that two or more copies of the repeat in any relative orientation are able to perform the replicator function. A single copy of a MATE element increases transformation frequency to a modest extent but leads to multiple rearrangement, unstable integration or concatenation of vector molecules. Multimeric concatenates generated during this process are more sable mitotically, and when reisolated, transform the fungus at a much higher frequency than the original monomeric vector. Selection for multiple products which resemble amplified DNA in various eukaryotic systems.
journal_name
Mol Microbioljournal_title
Molecular microbiologyauthors
Aleksenko A,Gems D,Clutterbuck Jdoi
10.1111/j.1365-2958.1996.tb02629.xsubject
Has Abstractpub_date
1996-04-01 00:00:00pages
427-34issue
2eissn
0950-382Xissn
1365-2958journal_volume
20pub_type
杂志文章abstract::Enteropathogenic Escherichia coli (EPEC) is a leading cause of infant diarrhoea. EPEC mediates several effects on host epithelial cells, including activation of signal-transduction pathways, cytoskeletal rearrangement along with pedestal and attaching/effacing lesion formation. It has been previously shown that the EP...
journal_title:Molecular microbiology
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更新日期:1995-04-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:1996-03-01 00:00:00
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更新日期:2005-08-01 00:00:00
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pub_type: 杂志文章
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更新日期:2005-11-01 00:00:00
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更新日期:1999-07-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:1996-04-01 00:00:00
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