Abstract:
:A search was performed for a periplasmic molecular chaperone which may assist outer membrane proteins of Escherichia coli on their way from the cytoplasmic to the outer membrane. Proteins of the periplasmic space were fractionated on an affinity column with sepharose-bound outer membrane porin OmpF. A 17 kDa polypeptide was the predominant protein retained by this column. The corresponding gene was found in a gene bank; it encodes the periplasmic protein Skp. The protein was isolated and it could be demonstrated that it bound outer membrane proteins, following SDS-PAGE, with high selectivity. Among these were OmpA, OmpC, OmpF and the maltoporin LamB. The chromosomal skp gene was inactivated by a deletion causing removal of most of the signal peptide plus 107 residues of the 141-residue mature protein. The mutant was viable but possessed much-reduced concentrations of outer membrane proteins. This defect was fully restored by a plasmid-borne skp gene which may serve as a periplasmic chaperone.
journal_name
Mol Microbioljournal_title
Molecular microbiologyauthors
Chen R,Henning Udoi
10.1111/j.1365-2958.1996.tb02473.xsubject
Has Abstractpub_date
1996-03-01 00:00:00pages
1287-94issue
6eissn
0950-382Xissn
1365-2958journal_volume
19pub_type
杂志文章abstract::Pseudomonas aeruginosa PAO1, an opportunistic human pathogen, deploys several strategies to resist antibiotics. It uses multidrug efflux pumps, including the MexAB-OprM pump, for antibiotic resistance, and it also produces hydrogen sulfide (H2 S) that provides some defense against antibiotics. MexR functions as a tran...
journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/mmi.14593
更新日期:2020-12-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2958.2008.06189.x
更新日期:2008-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:2004-06-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2958.2009.06691.x
更新日期:2009-05-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2958.2003.03850.x
更新日期:2004-01-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:1997-07-01 00:00:00
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pub_type: 杂志文章
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更新日期:2010-12-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2958.1995.tb02437.x
更新日期:1995-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:2007-01-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:1990-01-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2958.2011.07597.x
更新日期:2011-04-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:2019-04-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:2001-08-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:1989-03-01 00:00:00
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pub_type: 杂志文章
doi:10.1046/j.1365-2958.2000.02012.x
更新日期:2000-08-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2958.2011.07869.x
更新日期:2011-11-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/mmi.14314
更新日期:2019-09-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:1987-07-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:2000-04-01 00:00:00
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journal_title:Molecular microbiology
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journal_title:Molecular microbiology
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更新日期:1997-07-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:2005-02-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:2020-10-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/mmi.14414
更新日期:2020-01-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2958.2002.03045.x
更新日期:2002-08-01 00:00:00
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pub_type: 杂志文章
doi:10.1046/j.1365-2958.2002.02916.x
更新日期:2002-04-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:1998-07-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:2006-09-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2958.2004.04154.x
更新日期:2004-07-01 00:00:00
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pub_type: 杂志文章
doi:10.1111/j.1365-2958.2009.06985.x
更新日期:2010-01-01 00:00:00