A novel two-gene requirement for the octanoyltransfer reaction of Bacillus subtilis lipoic acid biosynthesis.

abstract：:The interaction of Neisseria meningitidis with the meninges that surround and protect the brain is a pivotal event in the progression of bacterial meningitis. Two models of the human meninges were established in vitro, using (i) sections of fresh human brain and (ii) cultures of viable cells grown from human meningiom...

journal_title：Molecular microbiology

authors： Hardy SJ,Christodoulides M,Weller RO,Heckels JE

更新日期：2000-05-01 00:00:00

abstract：:Salmonella typhimurium responds to a variety of environmental stresses by accumulating the alternative sigma factor sigmaS. The repertoire of sigmaS -dependent genes that are subsequently expressed confers tolerance to a variety of potentially lethal conditions including low pH and stationary phase. The mechanism(s) r...

journal_title：Molecular microbiology

authors： Webb C,Moreno M,Wilmes-Riesenberg M,Curtiss R 3rd,Foster JW

更新日期：1999-10-01 00:00:00

abstract：:Escherichia coli RNA polymerase is able to site-specifically melt 12 bp of promoter DNA at temperatures far below those normally associated with DNA melting. Here we consider several models to explain how RNA polymerase destabilizes duplex DNA. One popular model proposes that upon binding to the promoter, RNA polymera...

journal_title：Molecular microbiology

authors： deHaseth PL,Helmann JD

更新日期：1995-06-01 00:00:00

abstract：:Expression from the Escherichia coli nrf operon promoter is activated by the anaerobically triggered transcription factor, FNR, and by the nitrate/nitrite ion-controlled response regulators, NarL or NarP, but is repressed by the IHF and Fis proteins. Here, we present in vitro studies on the nrf promoter, using permang...

journal_title：Molecular microbiology

authors： Browning DF,Grainger DC,Beatty CM,Wolfe AJ,Cole JA,Busby SJ

更新日期：2005-07-01 00:00:00

abstract：:Bacterial surface layers (S-layers) are extracellular protein networks that act as molecular sieves and protect a large variety of archaea and bacteria from hostile environments. Atomic force microscopy (AFM) was used to asses the S-layer of Coryne-bacterium glutamicum formed of PS2 proteins that assemble into hexamer...

journal_title：Molecular microbiology

authors： Scheuring S,Stahlberg H,Chami M,Houssin C,Rigaud JL,Engel A

更新日期：2002-05-01 00:00:00

abstract：:The recombination of short homologous ends in Escherichia coli has been known for 30 years, and it is often used for both site-directed mutagenesis and in vivo cloning. For cloning, a plasmid and target DNA fragments were converted into linear DNA fragments with short homologous ends, which are joined via recombinatio...

journal_title：Molecular microbiology

authors： Yang Y,Wang T,Yu Q,Liu H,Xun L,Xia Y

更新日期：2020-12-29 00:00:00

abstract：:The assembly of the class 5 colonization factor antigen I (CFA/I) fimbriae of enterotoxigenic E. coli was proposed to proceed via the alternate chaperone-usher pathway. Here, we show that in the absence of the chaperone CfaA, CfaB, the major pilin subunit of CFA/I fimbriae, is able to spontaneously refold and polymeri...

journal_title：Molecular microbiology

authors： Bao R,Liu Y,Savarino SJ,Xia D

更新日期：2016-12-01 00:00:00

abstract：:The creA gene of A. nidulans encodes a wide-domain regulatory protein mediating carbon catabolite repression. Northern blot analysis of creA mRNA revealed a complex expression profile: the addition of monosaccharides to a carbon-starved culture of A. nidulans provoked a strong transient stimulation of creA transcript ...

journal_title：Molecular microbiology

authors： Strauss J,Horvath HK,Abdallah BM,Kindermann J,Mach RL,Kubicek CP

更新日期：1999-04-01 00:00:00

abstract：:Dps is a nucleoid-associated protein that plays a major role in condensation of the Escherichia coli chromosome in stationary phase. Here we show that two other nucleoid-associated proteins, Fis and H-NS, can bind at the dps gene promoter and downregulate its activity. Both Fis and H-NS selectively repress the dps pro...

journal_title：Molecular microbiology

authors： Grainger DC,Goldberg MD,Lee DJ,Busby SJ

更新日期：2008-06-01 00:00:00

abstract：:The Leishmania major aquaglyceroporin, LmAQP1, is responsible for the transport of trivalent metalloids, arsenite and antimonite. We have earlier shown that downregulation of LmAQP1 provides resistance to trivalent antimony compounds whereas increased expression of LmAQP1 in drug-resistant parasites can reverse the re...

journal_title：Molecular microbiology

authors： Figarella K,Uzcategui NL,Zhou Y,LeFurgey A,Ouellette M,Bhattacharjee H,Mukhopadhyay R

更新日期：2007-08-01 00:00:00

abstract：:Superoxide dismutase, catalase, glutathione peroxidase and peroxiredoxins form an antioxidant network protecting cells against reactive oxygen species (ROS). Catalase is a potent H2O2-detoxifying enzyme, which is unexpectedly absent in some members of the Kinetoplastida and Apicomplexa, but present in Toxoplasma gondi...

journal_title：Molecular microbiology

authors： Ding M,Kwok LY,Schlüter D,Clayton C,Soldati D

更新日期：2004-01-01 00:00:00

abstract：:A unique 373 bp region (igr66) between grpE and dnaK of Streptococcus mutans lacks a promoter but is required for optimal production of DnaK. Northern blotting using probes specific to hrcA, igr66 or dnaK revealed multiple transcripts produced from the dnaK operon and 5'-RACE mapped 5' termini of multiple dnaK transcr...

journal_title：Molecular microbiology

authors： Palmer SR,Burne RA

更新日期：2015-10-01 00:00:00

abstract：:In bacteria, DNA supercoil movement is restricted to subchromosomal regions or 'domains.' To elucidate the nature of domain boundaries, we analysed reaction kinetics for gammadelta site-specific resolution in six chromosomal intervals ranging in size from 14 to 90 kb. In stationary cultures of Salmonella typhimurium, ...

journal_title：Molecular microbiology

authors： Staczek P,Higgins NP

更新日期：1998-09-01 00:00:00

abstract：:Only one species of Shigella, Shigella dysenteriae 1, has been demonstrated to produce Shiga toxin (Stx). Stx is closely related to the toxins produced by Shiga toxin-producing Escherichia coli (STEC). In STEC, these toxins are often encoded on lambdoid bacteriophages and are major virulence factors for these organism...

journal_title：Molecular microbiology

authors： McDonough MA,Butterton JR

更新日期：1999-12-01 00:00:00

abstract：:The translocation of the diphtheria toxin catalytic domain from the lumen of early endosomes into the cytosol of eukaryotic cells is an essential step in the intoxication process. We have previously shown that the in vitro translocation of the catalytic domain from the lumen of toxin pre-loaded endosomal vesicles to t...

journal_title：Molecular microbiology

authors： Trujillo C,Taylor-Parker J,Harrison R,Murphy JR

更新日期：2010-05-01 00:00:00

abstract：:The Bacillus subtilis ssuBACD gene cluster is required for the utilization of aliphatic sulphonates as sulphur sources. The regulation of expression of the ssu genes was studied in constructs carrying chromosomal transcriptional fusions of the ssu promoter region to lacZ. When sulphate or cystine served as sulphur sou...

journal_title：Molecular microbiology

authors： van der Ploeg JR,Barone M,Leisinger T

更新日期：2001-03-01 00:00:00

abstract：:We found that mutations that increased the transcription of the mgtCBR (Mg2+ transport-related) operon conferred increased thermotolerance on this organism. The 5' leader of the mgtCBR mRNA contains two short open reading frames (ORFs), mgtM and mgtP, whose translation regulates the expression of the mgtCBR operon by ...

journal_title：Molecular microbiology

authors： Gall AR,Hegarty AE,Datsenko KA,Westerman RP,SanMiguel P,Csonka LN

更新日期：2018-08-01 00:00:00

abstract：:An 18 633 bp region containing the replicon from the approximately 53 kb pBM400 plasmid of Bacillus megaterium QM B1551 has been sequenced and characterized. This region contained a complete rRNA operon plus 10 other potential open reading frames (ORFs). The replicon consisted of an upstream promoter and three contigu...

journal_title：Molecular microbiology

authors： Kunnimalaiyaan M,Stevenson DM,Zhou Y,Vary PS

更新日期：2001-02-01 00:00:00

abstract：:Pseudomonas syringae pv. tomato (Pst) strain DC3000 infects the model plants Arabidopsis thaliana and tomato, causing disease symptoms characterized by necrotic lesions surrounded by chlorosis. One mechanism used by Pst DC3000 to infect host plants is the type III protein secretion system, which is thought to deliver ...

journal_title：Molecular microbiology

authors： Zwiesler-Vollick J,Plovanich-Jones AE,Nomura K,Bandyopadhyay S,Joardar V,Kunkel BN,He SY

更新日期：2002-09-01 00:00:00

abstract：:Expression of the cysteine regulon in Salmonella typhimurium and Escherichia coli is controlled by the LysR-type transcriptional activator CysB and by the inducer N-acetyl-L-serine. Sulphide and thiosulphate are anti-inducers. Two highly purified constitutive CysB proteins, CysB(T149M) and CysB(T149P), were found to b...

journal_title：Molecular microbiology

authors： Colyer TE,Kredich NM

更新日期：1996-07-01 00:00:00

abstract：:The analysis of completed archaeal genome sequences led to the identification of a set of approximately 10-20 genes whose protein products were inferred to be involved in chromosomal DNA replication. Until recently, however, little was known of the biochemical properties of these proteins. Here, I review recent progre...

journal_title：Molecular microbiology

authors： MacNeill SA

更新日期：2001-05-01 00:00:00

abstract：:Populations of surface-attached microorganisms comprising either single or multiple species are commonly referred to as biofilms. Using a simple assay for the initiation of biofilm formation (e.g. attachment to an abiotic surface) by Pseudomonas fluorescens strain WCS365, we have shown that: (i) P. fluorescens can for...

journal_title：Molecular microbiology

authors： O'Toole GA,Kolter R

更新日期：1998-05-01 00:00:00

abstract：:Pseudomonas putida WCS358 can transport iron complexed to a wide variety of pseudobactins produced by other Pseudomonas strains. The pupB gene encoding an outer membrane ferric-pseudobactin receptor was isolated from a genomic library of P. putida WCS358. The PupB receptor facilitated iron transport via two distinct h...

journal_title：Molecular microbiology

authors： Koster M,van de Vossenberg J,Leong J,Weisbeek PJ

更新日期：1993-05-01 00:00:00

abstract：:We report here the cloning of the arginine repressor gene argR of Bacillus stearothermophilus and the characterization and purification to homogeneity of its product. The deduced amino acid sequence of the 16.8-kDa ArgR subunit shares 72% identity with its mesophilic homologue AhrC of Bacilus subtilis. Sequence analys...

journal_title：Molecular microbiology

authors： Dion M,Charlier D,Wang H,Gigot D,Savchenko A,Hallet JN,Glansdorff N,Sakanyan V

更新日期：1997-07-01 00:00:00

abstract：:Chemotactic adaptation to persisting stimulation involves reversible methylation of the chemoreceptors that form complexes with the histidine kinase CheA at a cell pole. The methyltransferase CheR targets to the C-terminal NWETF sequence of the chemoreceptor. In contrast, localization of the methylesterase CheB is lar...

journal_title：Molecular microbiology

authors： Banno S,Shiomi D,Homma M,Kawagishi I

更新日期：2004-08-01 00:00:00

abstract：:One often compares cells to computers, and signalling proteins to transistors. Location and wiring of those molecular transistors is paramount in defining the function of the subcellular chips. The bacterial chemotactic sensing apparatus is a large, stable assembly consisting of thousands of receptors, signal transduc...

journal_title：Molecular microbiology

authors： Shimizu TS,Le Novère N

更新日期：2008-07-01 00:00:00

abstract：:In the chemotaxis of Escherichia coli, adaptation requires the methylation and demethylation of transmembrane receptors, which are catalysed by the methyltransferase CheR and the methylesterase CheB respectively. CheR binds to major chemoreceptors through their C-terminal motif NWETF, which is distinct from the methyl...

journal_title：Molecular microbiology

authors： Shiomi D,Okumura H,Homma M,Kawagishi I

更新日期：2000-04-01 00:00:00

abstract：:The endoribonuclease RNase E plays an important role in RNA processing and degradation in Escherichia coli. The construction of an E. coli strain in which the cellular concentration of RNase E can be precisely controlled has made it possible to examine and quantify the effect of RNase E scarcity on RNA decay, gene reg...

journal_title：Molecular microbiology

authors： Jain C,Deana A,Belasco JG

更新日期：2002-02-01 00:00:00

abstract：:SpollE is an integral membrane protein that governs the establishment of cell-specific gene transcription during the process of sporulation in Bacillus subtilis. Synthesis of SpollE commences shortly after the onset of sporulation, after which the protein localizes at sites of potential cell division near both ends of...

journal_title：Molecular microbiology

authors： Levin PA,Losick R,Stragier P,Arigoni F

更新日期：1997-09-01 00:00:00

abstract：:The complete nucleotide sequence of the gene for a cell-surface protein antigen (PAc) of Streptococcus mutans MT8148 (serotype c) was determined. The pac gene consisted of 4695 bp and coded for a 170773D protein. The pac gene product contained a putative 38 amino acid signal peptide, resulting in a 166817D mature prot...

journal_title：Molecular microbiology

authors： Okahashi N,Sasakawa C,Yoshikawa M,Hamada S,Koga T

更新日期：1989-05-01 00:00:00