Abstract:
:Virion proteins recognize their cognate nucleic acid for encapsidation into virions through recognition of a specific nucleotide sequence contained within that nucleic acid. Viruses like bacteriophage P22, which have partially circularly permuted, double-stranded virion DNAs, encapsidate DNA through processive series of packaging events in which DNA is recognized for packaging only once at the beginning of the series. Thus a single DNA recognition event programmes the encapsidation of multiple virion chromosomes. The protein product of P22 gene 3, a terminase component, is thought to be responsible for this recognition. The site on the P22 genome that is recognized by the gene 3 protein to initiate packaging series is called the pac site. We report here a strategy for assaying pac site activity in vivo, and the utilization of this system to identify and characterize the site genetically. It is an asymmetric site that spans 22 basepairs and is located near the centre of P22 gene 3.
journal_name
Mol Microbioljournal_title
Molecular microbiologyauthors
Wu H,Sampson L,Parr R,Casjens Sdoi
10.1046/j.1365-2958.2002.03114.xsubject
Has Abstractpub_date
2002-09-01 00:00:00pages
1631-46issue
6eissn
0950-382Xissn
1365-2958pii
3114journal_volume
45pub_type
杂志文章abstract::Platelet binding by Streptococcus gordonii strain M99 is strongly correlated with the expression of the large surface glycoprotein GspB. A 14 kb chromosomal region downstream of gspB was previously shown to be required for the expression of this protein. The region encodes SecA2 and SecY2, which are components of an a...
journal_title:Molecular microbiology
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journal_title:Molecular microbiology
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pub_type: 评论,杂志文章
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更新日期:1994-07-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2958.1996.393934.x
更新日期:1996-02-01 00:00:00
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pub_type: 杂志文章
doi:10.1046/j.1365-2958.2001.02531.x
更新日期:2001-07-01 00:00:00
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pub_type: 杂志文章
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更新日期:2010-12-01 00:00:00
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