traJ sense RNA initiates at two different promoters in R100-1 and forms two stable hybrids with antisense finP RNA.

Abstract:

:RNase protection experiments show that the sizes of the two R100 finP molecules are 74 and 135 nucleotides. In an RNase III mutant, finP transcripts form stable double-stranded hybrids of 108 bp and 68 bp with traJ transcripts. RNase protection experiments also show that most R100-1 transcripts originating in traM cross the traM-traJ intergenic region and end inside the untranslated leader region of traJ. Some extend into the traJ open reading frame. These findings mean that the antisense finP RNA, thought to regulate traJ translation, must regulate traJ transcripts from both J and M promoters.

journal_name

Mol Microbiol

journal_title

Molecular microbiology

authors

Dempsey WB

doi

10.1111/j.1365-2958.1994.tb00425.x

subject

Has Abstract

pub_date

1994-07-01 00:00:00

pages

313-26

issue

2

eissn

0950-382X

issn

1365-2958

journal_volume

13

pub_type

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