Cis-acting signals controlling translational initiation in the thermophilic archaeon Sulfolobus solfataricus.

abstract：:5'-nucleotidases (5'-NTs) are enzymes that catalyze the hydrolysis of nucleoside monophosphates to produce nucleosides and phosphate. Since the identification of adenosine synthase A (AdsA) in Staphylococcus aureus in 2009, several other 5'-NTs have been discovered in Gram-positive cocci, mainly in streptococci. Despi...

journal_title：Molecular microbiology

authors： Soh KY,Loh JMS,Proft T

更新日期：2020-04-01 00:00:00

abstract：:An essential early event in Shigella and Salmonella pathogenesis is invasion of non-phagocytic intestinal epithelial cells. Pathogen entry is triggered by the delivery of multiple bacterial effector proteins into target mammalian cells. The Shigella invasion plasmid antigen B (IpaB), which inserts into the host plasma...

journal_title：Molecular microbiology

authors： Hume PJ,McGhie EJ,Hayward RD,Koronakis V

更新日期：2003-07-01 00:00:00

abstract：:Peptide transport in microorganisms is important for nutrition of the cell and various signalling processes including regulation of gene expression, sporulation, chemotaxis, competence and virulence development. Peptide transport is mediated via different combinations of ion-linked and ATP-binding cassette (ABC) trans...

journal_title：Molecular microbiology

authors： Doeven MK,Kok J,Poolman B

更新日期：2005-08-01 00:00:00

abstract：:Genes acrAB encode a multidrug efflux pump in Escherichia coli. We have previously reported that transcription of acrAB is increased under general stress conditions (i.e. 4% ethanol, 0.5 M NaCl, and the stationary phase in Luria-Bertani medium). In this study, lacZ transcriptional fusions and an in vitro gel mobility ...

journal_title：Molecular microbiology

authors： Ma D,Alberti M,Lynch C,Nikaido H,Hearst JE

更新日期：1996-01-01 00:00:00

abstract：:Type IV secretion systems are increasingly recognized as important virulence determinants of Gram-negative bacterial pathogens. While the examination of several type IV-secreted proteins suggested that their secretion depends on C-terminal signals, the nature of these signals and their conservation among different sys...

journal_title：Molecular microbiology

authors： Hohlfeld S,Pattis I,Püls J,Plano GV,Haas R,Fischer W

更新日期：2006-03-01 00:00:00

abstract：:The proton-driven flagellar motor of Salmonella enterica can accommodate a dozen MotA/B stators in a load-dependent manner. The C-terminal periplasmic domain of MotB acts as a structural switch to regulate the number of active stators in the motor in response to load change. The cytoplasmic loop termed MotAC is respon...

journal_title：Molecular microbiology

authors： Pourjaberi SNS,Terahara N,Namba K,Minamino T

更新日期：2017-11-01 00:00:00

abstract：:Klebsiella pullulanase is a lipoprotein synthesized as a precursor with a signal peptide, which is processed by lipoprotein signal peptidase. To clarify the role of lipid modification of pullulanase, we purified lipid-modified wild-type and the unmodified (mutant) pullulanases and compared their properties. The Km and...

journal_title：Molecular microbiology

authors： Yamashita M,Nakagawa A,Katsuragi N,Murooka Y

更新日期：1992-02-01 00:00:00

abstract：:Cell division in prokaryotes initiates with assembly of the Z-ring at midcell, which, in Escherichia coli, is tethered to the inner leaflet of the cytoplasmic membrane through a direct interaction with FtsA, a widely conserved actin homolog. The Z-ring is comprised of polymers of tubulin-like FtsZ and has been suggest...

journal_title：Molecular microbiology

authors： Conti J,Viola MG,Camberg JL

更新日期：2018-02-01 00:00:00

abstract：:RecA2278-5 is a mutant RecA protein (RecAmut) bearing two amino acid substitutions, Gly-278 to Thr and Val-275 to Phe, in the alpha-helix H of the C-terminal subdomain of the protein. RecA2278-5 mutant cells are unusual in that they are thermosensitive for recombination but almost normal for DNA repair of UV damage an...

journal_title：Molecular microbiology

authors： Alexseyev AA,Baitin DM,Kuramitsu S,Ogawa T,Ogawa H,Lanzov VA

更新日期：1997-01-01 00:00:00

abstract：:Programmed frameshifting in the RF2 gene (prfB) involves an intragenic Shine-Dalgarno (SD) sequence. To investigate the role of SD-ASD pairing in the mechanism of frameshifting, we have analysed the effect of spacing between the SD sequence and P codon on P-site tRNA binding and RF2-dependent termination. When the spa...

journal_title：Molecular microbiology

authors： Devaraj A,Fredrick K

更新日期：2010-12-01 00:00:00

abstract：:Campylobacter jejuni, a prevalent cause of bacterial gastroenteritis, must adapt to different environments to be a successful pathogen. We previously identified a C. jejuni two-component regulatory system (Cj1226/7c) as upregulated during cell infections. Analyses described herein led us to designate the system CprRS ...

journal_title：Molecular microbiology

authors： Svensson SL,Davis LM,MacKichan JK,Allan BJ,Pajaniappan M,Thompson SA,Gaynor EC

更新日期：2009-01-01 00:00:00

abstract：:We recently described the use of selective transposon mutagenesis to generate a series of avirulent mutants of a pathogenic strain of Salmonella typhimurium. Cloning and sequencing of the insertion sites from two of these mutants reveals that both have identical locations within an open reading frame that is highly ho...

journal_title：Molecular microbiology

authors： Johnson K,Charles I,Dougan G,Pickard D,O'Gaora P,Costa G,Ali T,Miller I,Hormaeche C

更新日期：1991-02-01 00:00:00

abstract：:A Rhizobium phaseoli cytochrome mutant, unable to oxidize N,N,N',N'-tetramethyl-p-phenylenediamine (TMPD), was isolated after Mu-dl (Kan lac) mutagenesis of the wild-type strain CE-3. Mutant strain CFN4202 had sixfold less haem-c but similar levels of b type, o and aa3 cytochromes than the wild-type strain. CFN4202 st...

journal_title：Molecular microbiology

authors： Soberón M,Aguilar GR,Sánchez F

更新日期：1993-04-01 00:00:00

abstract：:The bioluminescent marine bacterium Vibrio harveyi controls light production (lux) by an elaborate quorum-sensing circuit. V. harveyi produces and responds to two different autoinducer signals (AI-1 and AI-2) to modulate the luciferase structural operon (luxCDABEGH) in response to changes in cell-population density. U...

journal_title：Molecular microbiology

authors： Lilley BN,Bassler BL

更新日期：2000-05-01 00:00:00

abstract：:Neuroinvasive Escherichia coli K1 synthesizes and assembles a polysialic acid capsule virulence factor on the external leaflet of the outer membrane. This capsule functions in pathogenesis by blocking non-immune host defence mechanisms and acting as a relatively non-immunogenic molecular mimic of the polysialic acid c...

journal_title：Molecular microbiology

authors： Cieslewicz M,Vimr E

更新日期：1997-10-01 00:00:00

abstract：:Small regulatory RNAs (sRNAs) are well known to command bacterial protein synthesis by modulating the translation and decay of target mRNAs. Most sRNAs are specifically regulated by a cognate transcription factor under certain growth or stress conditions. Investigations of the conserved Hfq-dependent MicM sRNA in Esch...

journal_title：Molecular microbiology

authors： Vogel J

更新日期：2009-09-01 00:00:00

abstract：:Cytoadherence to the vaginal epithelium is a critical step in infection by the eukaryotic flagellate Trichomonas vaginalis. Four trichomonad surface proteins (AP65, AP51, AP33 and AP23) mediate cytoadherence. The cDNA encoding the AP65 adhesin was isolated from a phagemid cDNA expression library by screening with anti...

journal_title：Molecular microbiology

authors： Alderete JF,O'Brien JL,Arroyo R,Engbring JA,Musatovova O,Lopez O,Lauriano C,Nguyen J

更新日期：1995-07-01 00:00:00

abstract：:Coli surface antigen 6 (CS6) is a widely expressed enterotoxigenic Escherichia coli (ETEC) colonization factor that mediates bacterial attachment to the small intestinal epithelium. CS6 is a polymer of two protein subunits CssA and CssB, which are secreted and assembled on the cell surface via the CssC/CssD chaperone ...

journal_title：Molecular microbiology

authors： Roy SP,Rahman MM,Yu XD,Tuittila M,Knight SD,Zavialov AV

更新日期：2012-12-01 00:00:00

abstract：:The streptococcal antigen I/II (AgI/II)-family polypeptides are cell wall-anchored adhesins expressed by most indigenous oral streptococci. Proteins sharing 30-40% overall amino acid sequence similarities with AgI/II-family proteins are also expressed by Streptococcus pyogenes. The S. pyogenes M28_Spy1325 polypeptide ...

journal_title：Molecular microbiology

authors： Maddocks SE,Wright CJ,Nobbs AH,Brittan JL,Franklin L,Strömberg N,Kadioglu A,Jepson MA,Jenkinson HF

更新日期：2011-08-01 00:00:00

abstract：:The utilization of several sugars in Escherichia coli is regulated by the Phosphotransferase System (PTS), in which diverse sugar utilization modules compete for phosphoryl flux from the general PTS proteins. Existing theoretical work predicts a winner-take-all outcome when this flux limits carbon uptake. To date, no ...

journal_title：Molecular microbiology

authors： Westermayer SA,Fritz G,Gutiérrez J,Megerle JA,Weißl MP,Schnetz K,Gerland U,Rädler JO

更新日期：2016-05-01 00:00:00

abstract：:The Salmonella pathogenicity island (SPI-1) encodes approximately 35 proteins involved in assembly of a type III secretion system (T3SS) which endows Salmonella with the ability to invade eukaryotic cells. We have discovered a novel SPI-1 gene, invR, which expresses an abundant small non-coding RNA (sRNA). The invR ge...

journal_title：Molecular microbiology

authors： Pfeiffer V,Sittka A,Tomer R,Tedin K,Brinkmann V,Vogel J

更新日期：2007-12-01 00:00:00

abstract：:Among enteric bacteria, the ability to grow on N-acetyl-galactosamine (GalNAc or Aga) and on D-galactosamine (GalN or Gam) differs. Thus, strains B, C and EC3132 of Escherichia coli are Aga+ Gam+ whereas E. coli K-12 is Aga- Gam-, similarly to Klebsiella pneumoniae KAY2026, Klebsiella oxytoca M5a1 and Salmonella typhi...

journal_title：Molecular microbiology

authors： Brinkkötter A,Klöss H,Alpert C,Lengeler JW

更新日期：2000-07-01 00:00:00

abstract：:Pseudomonas aeruginosa, a Gram-negative bacterium, is a significant opportunistic pathogen associated with skin and soft tissue infections, nosocomial pneumonia and sepsis. In addition, it can chronically colonize the lungs of cystic fibrosis (CF) patients. Overproduction of the exopolysaccharide called alginate provi...

journal_title：Molecular microbiology

authors： Damron FH,Goldberg JB

更新日期：2012-05-01 00:00:00

abstract：:Lipid metabolism in Trypanosoma brucei, the causative agent of African sleeping sickness, differs from its human host in several fundamental ways. This has lead to the validation of a plethora of novel drug targets, giving hope of novel chemical intervention against this neglected disease. Cytidine diphosphate diacylg...

journal_title：Molecular microbiology

authors： Lilley AC,Major L,Young S,Stark MJ,Smith TK

更新日期：2014-05-01 00:00:00

abstract：:The bacterium Burkholderia glumae causes rice grain rot by producing toxoflavin, whose expression is regulated by quorum sensing (QS). We report a major deviation from the current paradigm for the regulation of bacterial polar flagellum genes. The N-octanoyl homoserine lactone (C8-HSL)-deficient mutant of B. glumae is...

journal_title：Molecular microbiology

authors： Kim J,Kang Y,Choi O,Jeong Y,Jeong JE,Lim JY,Kim M,Moon JS,Suga H,Hwang I

更新日期：2007-04-01 00:00:00

abstract：:Invasive sporozoite and merozoite stages of malaria parasites that infect mammals enter and subsequently reside in hepatocytes and red blood cells respectively. Each invasive stage may exhibit unique adaptations that allow it to interact with and survive in its distinct host cell environment, and these adaptations are...

journal_title：Molecular microbiology

authors： Kaiser K,Matuschewski K,Camargo N,Ross J,Kappe SH

更新日期：2004-03-01 00:00:00

abstract：:Teichoic acids (TAs) are the most abundant glycopolymers in the cell wall of Listeria, an opportunistic Gram-positive pathogen that causes severe foodborne infections. Two different structural classes of Listeria TA exist: the polyribitolphosphate-based wall teichoic acid (WTA) that is covalently anchored to the pepti...

journal_title：Molecular microbiology

authors： Sumrall ET,Keller AP,Shen Y,Loessner MJ

更新日期：2020-03-01 00:00:00

abstract：SUMMARY:The conserved bacterial protein RloC, a distant homologue of the tRNA(Lys) anticodon nuclease (ACNase) PrrC, is shown here to act as a wobble nucleotide-excising and Zn(++)-responsive tRNase. The more familiar PrrC is silenced by a genetically linked type I DNA restriction-modification (R-M) enzyme, activated b...

journal_title：Molecular microbiology

authors： Davidov E,Kaufmann G

更新日期：2008-09-01 00:00:00

abstract：:Gut bacteria recognize accessible glycan substrates within a complex environment. Carbohydrate binding modules (CBMs) of cell surface glycoside hydrolases often drive binding to the target substrate. Eubacterium rectale, an important butyrate-producing organism in the gut, consumes a limited range of substrates, inclu...

journal_title：Molecular microbiology

authors： Cockburn DW,Suh C,Medina KP,Duvall RM,Wawrzak Z,Henrissat B,Koropatkin NM

更新日期：2018-01-01 00:00:00

abstract：:We report here the cloning of the arginine repressor gene argR of Bacillus stearothermophilus and the characterization and purification to homogeneity of its product. The deduced amino acid sequence of the 16.8-kDa ArgR subunit shares 72% identity with its mesophilic homologue AhrC of Bacilus subtilis. Sequence analys...

journal_title：Molecular microbiology

authors： Dion M,Charlier D,Wang H,Gigot D,Savchenko A,Hallet JN,Glansdorff N,Sakanyan V

更新日期：1997-07-01 00:00:00