Differential transcriptome profiling identifies Plasmodium genes encoding pre-erythrocytic stage-specific proteins.

abstract：:Using a previously developed expression system based on the erythromycin-producing strain of Saccharopolyspora erythraea, O-methyltransferases from the spinosyn biosynthetic gene cluster of Saccharopolyspora spinosa have been shown to modify a rhamnosyl sugar attached to a 14-membered polyketide macrolactone. The spnI...

journal_title：Molecular microbiology

authors： Gaisser S,Lill R,Wirtz G,Grolle F,Staunton J,Leadlay PF

更新日期：2001-09-01 00:00:00

abstract：:Proteinaceous, hair-like appendages known as fimbriae or pili commonly extend from the surface of prokaryotic cells and serve important functions such as cell adhesion, biofilm formation, motility and DNA transfer. Here we show that a novel group of archaea from cold, sulphidic springs has developed cell surface appen...

journal_title：Molecular microbiology

authors： Moissl C,Rachel R,Briegel A,Engelhardt H,Huber R

更新日期：2005-04-01 00:00:00

abstract：:The cell wall is a crucial structural feature in the vast majority of bacteria and comprises a covalently closed network of peptidoglycan (PG) strands. While PG synthesis is important for survival under many conditions, the cell wall is also a dynamic structure, undergoing degradation and remodeling by 'autolysins', e...

journal_title：Molecular microbiology

authors： Weaver AI,Jiménez-Ruiz V,Tallavajhala SR,Ransegnola BP,Wong KQ,Dörr T

更新日期：2019-10-01 00:00:00

abstract：:In general, wild Escherichia coli strains can grow effectively under moderately acidic organic acid-rich conditions. We found that the Shiga Toxin-producing E. coli (STEC) O157:H7 NGY9 grows more quickly than a K-12 strain in Luria-Bertani (LB)-2-morpholinoethanesulphonic acid (MES) broth supplemented with acetic acid...

journal_title：Molecular microbiology

authors： Barua S,Yamashino T,Hasegawa T,Yokoyama K,Torii K,Ohta M

更新日期：2002-02-01 00:00:00

abstract：:Bacteria tightly regulate intracellular zinc levels to ensure sufficient zinc to support essential functions, while preventing toxicity. The bacterial response to zinc limitation includes the expression of putative zinc metallochaperones belonging to subfamily 1 of the COG0523 family of G3E GTPases. However, the clien...

journal_title：Molecular microbiology

authors： Chandrangsu P,Huang X,Gaballa A,Helmann JD

更新日期：2019-09-01 00:00:00

abstract：:A collection of 20 strains of Helicobacter pylori from several regions of the world was studied to better understand the population genetic structure and diversity of this species. Sequences of fragments from seven housekeeping genes (atpA, efp, mutY, ppa, trpC, ureI, yphC ) and two virulence-associated genes (cagA, v...

journal_title：Molecular microbiology

authors： Achtman M,Azuma T,Berg DE,Ito Y,Morelli G,Pan ZJ,Suerbaum S,Thompson SA,van der Ende A,van Doorn LJ

更新日期：1999-05-01 00:00:00

abstract：:In Escherichia coli, RecA protein regulates the DNA damage-inducible survival-enhancing SOS response. Mutant allele recA730, which causes constitutive SOS expression, is lethal at high temperatures in B/r, a derivative of wild-type B, but not in K-12 or in certain B/r--K-12 hybrids. We present evidence that killing is...

journal_title：Molecular microbiology

authors： Kirchner J,Lim D,Witkin EM,Garvey N,Roegner-Maniscalco V

更新日期：1992-10-01 00:00:00

abstract：:The energy requirement for the second step in pullulanase secretion by the general secretory pathway was studied in Escherichia coli. In order to uncouple the two steps in the secretion pathway (across the cytoplasmic and outer membranes, respectively) and to facilitate kinetic analysis of secretion, a variant form of...

journal_title：Molecular microbiology

authors： Possot OM,Letellier L,Pugsley AP

更新日期：1997-05-01 00:00:00

abstract：:Only few small, regulatory RNAs encoded opposite another gene have been identified in bacteria. Here, we report the characterization of a locus where a small RNA (SymR) is encoded in cis to an SOS-induced gene whose product shows homology to the antitoxin MazE (SymE). Synthesis of the SymE protein is tightly repressed...

journal_title：Molecular microbiology

authors： Kawano M,Aravind L,Storz G

更新日期：2007-05-01 00:00:00

abstract：:Post-translational modifications account for much of the biological diversity generated at the proteome level. Of these, glycosylation is the most prevalent. Long thought to be unique to Eukarya, it is now clear that both Bacteria and Archaea are also capable of N-glycosylation, namely the covalent linkage of oligosac...

journal_title：Molecular microbiology

authors： Yurist-Doutsch S,Chaban B,VanDyke DJ,Jarrell KF,Eichler J

更新日期：2008-06-01 00:00:00

abstract：:We recently described the use of selective transposon mutagenesis to generate a series of avirulent mutants of a pathogenic strain of Salmonella typhimurium. Cloning and sequencing of the insertion sites from two of these mutants reveals that both have identical locations within an open reading frame that is highly ho...

journal_title：Molecular microbiology

authors： Johnson K,Charles I,Dougan G,Pickard D,O'Gaora P,Costa G,Ali T,Miller I,Hormaeche C

更新日期：1991-02-01 00:00:00

abstract：:A challenge common to all bacterial pathogens is to acquire nutrients from hostile host environments. Iron is an important cofactor required for essential cellular processes such as DNA repair, energy production and redox balance. Within a mammalian host, most iron is sequestered within heme, which in turn is predomin...

journal_title：Molecular microbiology

authors： Clark J,Terwilliger A,Nguyen C,Green S,Nobles C,Maresso A

更新日期：2019-08-01 00:00:00

abstract：:The DNA of wild-type Streptomyces lividans 66 is degraded during electrophoresis in buffers containing traces of ferrous iron. S. lividans ZX1, a mutant selected for resistance to DNA degradation, simultaneously became sensitive to phi HAU3, a wide-host-range temperate bacteriophage. A DNA fragment conferring phi HAU3...

journal_title：Molecular microbiology

authors： Zhou X,Deng Z,Hopwood DA,Kieser T

更新日期：1994-06-01 00:00:00

abstract：:Small repeat sequences in bacterial genomes, which represent non-autonomous mobile elements, have close similarities to archaeon and eukaryotic miniature inverted repeat transposable elements. These repeat elements are found in both intergenic and intragenic chromosomal regions, and contain an array of diverse motifs....

journal_title：Molecular microbiology

authors： Delihas N

更新日期：2008-02-01 00:00:00

abstract：:The Escherichia coli rodA and ftsW genes and the spoVE gene of Bacillus subtilis encode membrane proteins that control peptidoglycan synthesis during cellular elongation, division and sporulation respectively. While rodA and ftsW are essential genes in E. coli, the B. subtilis spoVE gene is dispensable for growth and ...

journal_title：Molecular microbiology

authors： Henriques AO,Glaser P,Piggot PJ,Moran CP Jr

更新日期：1998-04-01 00:00:00

abstract：:Transposon mutagenesis was used to isolate insertion mutants of the photosynthetic bacterium Rhodobacter capsulatus which were unable to grow under aerobic conditions in the dark on malate, succinate or fumarate as sole carbon sources. Of five mutants isolated, all were deficient in C4-dicarboxylate transport. However...

journal_title：Molecular microbiology

authors： Hamblin MJ,Shaw JG,Curson JP,Kelly DJ

更新日期：1990-09-01 00:00:00

abstract：:OmpR activates expression of the two-component regulatory system located on Salmonella pathogenicity island 2 (SPI-2) that controls the expression of a type III secretion system, as well as many other genes required for systemic infection in mice. Measurements of SsrA and SsrB protein levels under different growth con...

journal_title：Molecular microbiology

authors： Feng X,Walthers D,Oropeza R,Kenney LJ

更新日期：2004-11-01 00:00:00

abstract：:Factors that enhance the transmission of pathogens are poorly understood. We show that Vibrio cholerae shed in human 'rice-water' stools have a 10-fold lower oral infectious dose in an animal model than in vitro grown V. cholerae, which may aid in transmission during outbreaks. Furthermore, we identify a bacterial fac...

journal_title：Molecular microbiology

authors： Butler SM,Nelson EJ,Chowdhury N,Faruque SM,Calderwood SB,Camilli A

更新日期：2006-04-01 00:00:00

abstract：:XylR is a σ⁵⁴-dependent transcriptional factor of Pseudomonas putida that activates the Pu promoter of the TOL plasmid upon binding its natural effector, m-xylene. The search for mutants of the signal-sensing module of XylR that respond to the xenobiotic compound 2,4-dinitrotoluene recurrently yields protein variants ...

journal_title：Molecular microbiology

authors： de Las Heras A,de Lorenzo V

更新日期：2011-03-01 00:00:00

abstract：:Bacterial cell division requires the assembly of FtsZ protofilaments into a dynamic structure called the 'Z-ring'. The Z-ring recruits the division machinery and directs local cell wall remodeling for constriction. The organization and dynamics of protofilaments within the Z-ring coordinate local cell wall synthesis d...

journal_title：Molecular microbiology

authors： Sundararajan K,Vecchiarelli A,Mizuuchi K,Goley ED

更新日期：2018-10-01 00:00:00

abstract：:Replacement of the amino-terminal 40-amino-acid region of the 588-amino-acid precursor of the membrane-bound penicillin-binding protein 3 (PBP3) by the decapeptide MKGKEFQAWI was carried out by altering the amino-coding end of the ftsI gene. Insertion of the modified gene into a runaway-replication plasmid under the c...

journal_title：Molecular microbiology

authors： Belder JB,Nguyen-Distèche M,Houba-Herin N,Ghuysen JM,Maruyama IN,Hara H,Hirota Y,Inouye M

更新日期：1988-07-01 00:00:00

abstract：:In mid-June, the second Young Microbiologists Symposium took place under the broad title of 'Microbe signalling, organization and pathogenesis' on the picturesque campus of University College Cork, Ireland. The symposium attracted 150 microbiologists from 15 different countries. The key feature of this meeting was tha...

journal_title：Molecular microbiology

authors： Caly DL,Coulthurst SJ,Geoghegan JA,Malone JG,Ryan RP

更新日期：2012-11-01 00:00:00

abstract：:SigD and SigE (Salmonella invasion gene) are proteins needed for optimal invasion of Salmonella typhimurium into eukaryotic cells in vitro. SigD is a secreted protein and SigE is a putative chaperone required for SigD stability and/or secretion. SigD is secreted by a type III secretion apparatus encoded within a patho...

journal_title：Molecular microbiology

authors： Darwin KH,Miller VL

更新日期：2000-02-01 00:00:00

abstract：:The malaria parasite Plasmodium falciparum assembles knob structures underneath the erythrocyte membrane that help present the major virulence protein, P. falciparum erythrocyte membrane protein-1 (PfEMP1). Membranous structures called Maurer's clefts are established in the erythrocyte cytoplasm and function as sortin...

journal_title：Molecular microbiology

authors： Dixon MW,Kenny S,McMillan PJ,Hanssen E,Trenholme KR,Gardiner DL,Tilley L

更新日期：2011-08-01 00:00:00

abstract：:Toxoplasma gondii is an obligate intracellular protozoan parasite whose rapid lytic replication cycles define its pathogenicity. We identified a temperature-sensitive growth mutant, FV-P6, which irreversibly arrests before the middle of the G1 stage of the tachyzoite cell cycle. This arrest is caused by a point mutati...

journal_title：Molecular microbiology

authors： Szatanek T,Anderson-White BR,Faugno-Fusci DM,White M,Saeij JP,Gubbels MJ

更新日期：2012-05-01 00:00:00

abstract：:Lactose is an abundant dietary carbohydrate metabolized by the dental pathogen Streptococcus mutans. Lactose metabolism presents both classic diauxic behaviors and long-term memory, where the bacteria can pause for >11 h before initiating growth on lactose. Here, we explored mechanisms contributing to unusual aspects ...

journal_title：Molecular microbiology

authors： Zeng L,Burne RA

更新日期：2020-09-03 00:00:00

abstract：:We have shown previously that deletion of the Saccharomyces cerevisiae UMP1 gene encoding the 20S proteasome maturase causes sensitivity to UV radiation. In the current report, we have extended this finding to show that mutations specifically compromising chymotrypsin-like or trypsin-like activity of 20S proteasome pe...

journal_title：Molecular microbiology

authors： Podlaska A,McIntyre J,Skoneczna A,Sledziewska-Gojska E

更新日期：2003-09-01 00:00:00

abstract：:Pseudomonas aeruginosa can express two distinct forms of lipopolysaccharide (LPS), called A-band and B-band. As an attempt to understand the molecular biology of the synthesis and regulation of these LPS antigens, a recombinant plasmid, pFV3, containing genes for A-band expression was isolated previously. In the prese...

journal_title：Molecular microbiology

authors： Lightfoot J,Lam JS

更新日期：1993-05-01 00:00:00

abstract：:The interaction of Neisseria meningitidis with the meninges that surround and protect the brain is a pivotal event in the progression of bacterial meningitis. Two models of the human meninges were established in vitro, using (i) sections of fresh human brain and (ii) cultures of viable cells grown from human meningiom...

journal_title：Molecular microbiology

authors： Hardy SJ,Christodoulides M,Weller RO,Heckels JE

更新日期：2000-05-01 00:00:00

abstract：:The AbcR small RNAs (sRNAs) are a fascinating example of two highly conserved sRNAs that differ tremendously at the functional level among organisms. From their transcriptional activation to their regulatory capabilities, the AbcR sRNAs exhibit varying characteristics in three well-studied bacteria belonging to the Rh...

journal_title：Molecular microbiology

authors： Sheehan LM,Caswell CC

更新日期：2018-01-01 00:00:00