Abstract：
:We recently described the use of selective transposon mutagenesis to generate a series of avirulent mutants of a pathogenic strain of Salmonella typhimurium. Cloning and sequencing of the insertion sites from two of these mutants reveals that both have identical locations within an open reading frame that is highly homologous to a gene, htrA, encoding a heat-shock protein in Escherichia coli. DNA sequence analysis of S. typhimurium htrA reveals the presence of a gene capable of encoding a protein with a calculated Mr of 49316 that has 88.7% protein:protein homology with its E. coli counterpart. In E. coli, lesions in this gene, also known as degP, reduce proteolytic degradation of aberrant periplasmic proteins. Characteristics of the S. typhimurium htrA mutants, 046 and 014, in vivo and in vitro suggested that they are avirulent because of impaired ability to survive and/or replicate in host tissues. In vitro, the S. typhimurium htrA mutants 046 and 014 are not temperature-sensitive but were found to be more susceptible to oxidative stress than the parent, suggesting that they may be less able to withstand oxidative killing within macrophages.
journal_name
Mol Microbioljournal_title
Molecular microbiologyauthors
Johnson K,Charles I,Dougan G,Pickard D,O'Gaora P,Costa G,Ali T,Miller I,Hormaeche Cdoi
10.1111/j.1365-2958.1991.tb02122.xsubject
Has Abstractpub_date
1991-02-01 00:00:00pages
401-7issue
2eissn
0950-382Xissn
1365-2958journal_volume
5pub_type
杂志文章abstract：:Genetic recombination impacts on neisserial biology in two ways: (i) specific loci undergo rearrangement at high frequency leading to the formation of many different alleles; and (ii) Neisseria, being naturally competent for DNA transformation, provide a means to disseminate the novel alleles throughout a population. ...
journal_title：Molecular microbiology
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journal_title：Molecular microbiology
pub_type： 杂志文章
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journal_title：Molecular microbiology
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pub_type： 杂志文章
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更新日期：1998-08-01 00:00:00
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更新日期：2008-09-01 00:00:00
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更新日期：2012-12-01 00:00:00
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journal_title：Molecular microbiology
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