Abstract：
:Escherichia coli loses its rod shape by inactivation of PBP2 (penicillin-binding protein 2), target of the beta-lactam mecillinam. Under these conditions, cell division is blocked in rich medium. Division in the absence of PBP2 activity is restored (and resistance to mecillinam is conferred) when the three cell division proteins FtsQ, FtsA and FtsZ are overproduced, but not when only one or two of them are overproduced. Division in the absence of PBP2 activity is also restored by a doubling in the ppGpp pool, as in the argS201 mutant. However, the nucleotide ppGpp, a transcriptional regulator of many operons, does not govern any of the five promoters of the ftsQAZoperon, as shown by S1 mapping of ftsQAZ mRNA 5' ends in exponentially growing wild-type cells in the mecillinam-resistant argS201 mutant (intermediate ppGpp level) or during the stringent response elicited by isoleucine starvation (high ppGpp level). Furthermore, the concentration of FtsZ protein is not increased in exponentially growing mecillinam-resistant argS201 cells. These results show that the ftsQAZ operon is not the ppGpp target responsible for mecillinam resistance. We are currently trying to identify those targets that, at intermediate ppGpp levels, allow cells to divide as spheres in the absence of PBP2.
journal_name
Mol Microbioljournal_title
Molecular microbiologyauthors
Navarro F,Robin A,D'Ari R,Joseleau-Petit Ddoi
10.1046/j.1365-2958.1998.00974.xsubject
Has Abstractpub_date
1998-08-01 00:00:00pages
815-23issue
3eissn
0950-382Xissn
1365-2958journal_volume
29pub_type
杂志文章abstract：:The mechanisms of transcription repression and derepression in vivo are not fully understood. We have obtained evidence that begins to clarify the minimum requirements for counteracting nucleosomal repression in vivo. Location of the TATA element near the nucleosome dyad does not block RNA polymerase II transcription ...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2958.2000.01940.x
更新日期：2000-06-01 00:00:00
abstract：:Members of the bacterial genus Brucella are facultative intracellular pathogens that reside predominantly within membrane-bound compartments within two host cell types, macrophages and placental trophoblasts. Within macrophages, the brucellae route themselves to an intracellular compartment that is favourable for surv...
journal_title：Molecular microbiology
pub_type： 杂志文章,评审
doi：10.1111/j.1365-2958.2004.04017.x
更新日期：2004-05-01 00:00:00
abstract：:Neisseria meningitidis controls the expression of several genes involved in host adaptation by a process known as phase variation. The phase variation frequency of haemoglobin (Hb) receptors among clinical isolates of serogroups A, B and C differed drastically, ranging from approximately 10(-6) to 10(-2) cfu-1. Freque...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2958.2001.02408.x
更新日期：2001-05-01 00:00:00
abstract：:Poly(A) polymerase I (PAP I) of Escherichia coli is a member of the nucleotidyltransferase (Ntr) superfamily that includes the eukaryotic PAPs and all the known tRNA CCA-adding enzymes. Five highly conserved aspartic acids in the putative catalytic site of PAP I were changed to either alanine or proline, demonstrating...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2958.1999.01394.x
更新日期：1999-05-01 00:00:00
abstract：:Proteic toxin-antitoxin (TA) loci were first identified in bacterial plasmids, and they were regarded as involved in stable plasmid maintenance by a so-called 'addiction' mechanism. Later, chromosomally encoded TA loci were identified and their function ascribed to survival mechanisms when bacteria were subjected to s...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.2006.05027.x
更新日期：2006-02-01 00:00:00
abstract：:The stable maintenance of low-copy-number plasmids requires active partitioning, with the most common mechanism in prokaryotes involving the ATPase ParA. ParA proteins undergo intricate spatiotemporal relocations across the nucleoid, dynamics that function to position plasmids at equally spaced intervals. This spacing...
journal_title：Molecular microbiology
pub_type： 评论,杂志文章
doi：10.1111/j.1365-2958.2010.07316.x
更新日期：2010-10-01 00:00:00
abstract：:Expression of the Aspergillus nidulans 22 kDa endoxylanase gene, xlnA, is controlled by at least three mechanisms: specific induction by xylan or xylose; carbon catabolite repression (CCR); and regulation by ambient pH. Deletion analysis of xlnA upstream sequences has identified two positively acting regions: one that...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2958.1999.01157.x
更新日期：1999-01-01 00:00:00
abstract：:Virion proteins recognize their cognate nucleic acid for encapsidation into virions through recognition of a specific nucleotide sequence contained within that nucleic acid. Viruses like bacteriophage P22, which have partially circularly permuted, double-stranded virion DNAs, encapsidate DNA through processive series ...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2958.2002.03114.x
更新日期：2002-09-01 00:00:00
abstract：:A gene conferring low-level isoniazid (INH) resistance on Mycobacterium smegmatis was isolated from a cosmid library of the genome of an INH-resistant Mycobacterium bovis strain. The gene had good homology with ahpC, the product of which is a subunit of alkyl hydroperoxide reductase, and also with a family of thiol-sp...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2958.1996.449980.x
更新日期：1996-03-01 00:00:00
abstract：:Replacement of the amino-terminal 40-amino-acid region of the 588-amino-acid precursor of the membrane-bound penicillin-binding protein 3 (PBP3) by the decapeptide MKGKEFQAWI was carried out by altering the amino-coding end of the ftsI gene. Insertion of the modified gene into a runaway-replication plasmid under the c...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.1988.tb00058.x
更新日期：1988-07-01 00:00:00
abstract：:We screened the putative rap-phr (response regulator aspartyl-phosphate phosphatase-phosphatase regulator) systems identified in the Bacillus subtilis genome for a rap gene that affects aprE (alkaline protease gene) expression by using a multicopy plasmid. We found that rapG was involved in the regulation of aprE, whi...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2958.2003.03665.x
更新日期：2003-09-01 00:00:00
abstract：:Initiation of translation involves recognition of the start codon by the initiator tRNA in the 30S subunit. To investigate the role of ribosomal RNA (rRNA) in this process, we isolated a number of 16S rRNA mutations that increase translation from the non-canonical start codon AUC. These mutations cluster to distinct r...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.2009.06598.x
更新日期：2009-03-01 00:00:00
abstract：:Establishment of an axis of cell polarity and differentiation of the cell poles are fundamental aspects of cellular development in many organisms. We compared the effects of two bacterial cytoskeletal-like systems, the MreB and MinCDE systems, on these processes in Escherichia coli. We report that the Min proteins are...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.2005.04841.x
更新日期：2005-11-01 00:00:00
abstract：:The flagellar genes of Caulobacter crescentus are expressed under cell-cycle control. Expression is regulated by both flagellar assembly cues and cell-cycle events. In this paper we define the sequences required for the expression of the flgF operon, a new class of sigma 54 flagellar promoter. This promoter type is ex...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.1995.tb02300.x
更新日期：1995-04-01 00:00:00
abstract：:Bacteria react to phosphate starvation by activating genes involved in the transport and assimilation of phosphate as well as other phosphorous compounds. Some soil bacteria have evolved an additional mechanism for saving phosphorous. Under phosphate-limiting conditions, they replace their membrane phospholipids by li...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2958.1999.01325.x
更新日期：1999-04-01 00:00:00
abstract：:Malaria parasites scavenge nutrients from their host but also harbour enzymatic pathways for de novo macromolecule synthesis. One such pathway is apicoplast-targeted type II fatty acid synthesis, which is essential for late liver-stage development in rodent malaria. It is likely that fatty acids synthesized in the api...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/mmi.12485
更新日期：2014-02-01 00:00:00
abstract：:In order to identify molecules that might be responsible for the difference in pathogenicity between the two closely related protozoan parasites Entamoeba histolytica and Entamoeba dispar, we focussed on cysteine proteinases because this class of enzymes has been considered important for pathogenic tissue destruction....
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2958.1996.00111.x
更新日期：1996-10-01 00:00:00
abstract：:A screen for Mycobacterium tuberculosis (Mtb) mutants sensitive to reactive nitrogen intermediates identified transposon insertions in the presumptive proteasomal ATPase gene mpa (mycobacterium proteasome ATPase; Rv2115c). mpa mutants are attenuated in both wild type and nitric oxide synthase 2 deficient mice. In this...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.2004.04403.x
更新日期：2005-01-01 00:00:00
abstract：:Proteins with LytM (Peptidase_M23) domains are broadly distributed in bacteria and have been implicated in a variety of important processes, including cell division and cell-shape determination. Most LytM-like proteins that have been structurally and/or biochemically characterized are metallo-endopeptidases that cleav...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/mmi.12304
更新日期：2013-08-01 00:00:00
abstract：:The genome of the Bacillus subtilis 168-type strain contains 10 ribosomal RNA (rRNA) operons. In the intergenic spacer region (ISR) between the 16S and 23S rRNA genes, five rRNA operons, rrnI-H-G and rrnJ-W, lack a trinucleotide signature region. Precise determination of molecular weight (MW), using electrospray mass ...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2958.2001.02600.x
更新日期：2001-10-01 00:00:00
abstract：:We have used a targeted gene deletion event to remove the coding region for the bgl1 gene encoding an extracellular beta-glucosidase from the genome of the cellulolytic fungus Trichoderma reesei. The bgl1 null mutants were used to investigate the role of beta-glucosidase in the hydrolysis of cellulose and induction of...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.1992.tb01777.x
更新日期：1992-11-01 00:00:00
abstract：:Gram-negative bacteria possess several envelope stress responses that detect and respond to damage to this critical cellular compartment. The σ(E) envelope stress response senses the misfolding of outer membrane proteins (OMPs), while the Cpx two-component system is believed to detect the misfolding of periplasmic and...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/mmi.12581
更新日期：2014-05-01 00:00:00
abstract：:Several bacterial genera express proteins that contain collagen-like regions, which are associated with variable (V) non-collagenous regions. The streptococcal collagen-like proteins, Scl1 and Scl2, of group A Streptococcus (GAS) are members of this 'prokaryotic collagen' family, and they too contain an amino-terminal...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.2006.05237.x
更新日期：2006-07-01 00:00:00
abstract：:We used DNA macroarray analysis to identify genes that respond to the status of the intracellular acetyl phosphate (acP) pool. Genes whose expression correlated negatively with the ability to synthesize acP (i.e. negatively regulated genes) function primarily in flagella biosynthesis, a result consistent with observat...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2958.2003.03457.x
更新日期：2003-05-01 00:00:00
abstract：:A recurring theme in morphogenesis is the coupling of the expression of genes that drive morphogenesis and the morphogenetic process per se. This coupling ensures that gene expression and morphogenesis are carried out in synchrony. Morphogenesis of the spore-filled fruiting bodies in Myxococcus xanthus illustrates thi...
journal_title：Molecular microbiology
pub_type： 杂志文章,评审
doi：10.1046/j.1365-2958.2003.03399.x
更新日期：2003-04-01 00:00:00
abstract：:Protein quality control and proteolysis are involved in cell maintenance and environmental acclimatization in bacteria and eukaryotes. The AAA protease FtsH2 of the cyanobacterium Synechocystis sp. PCC 6803 was identified during a screening for mutants impaired in osmoregulation. The ftsH2(-) mutant was salt sensitive...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.2006.05495.x
更新日期：2007-01-01 00:00:00
abstract：:The Escherichia coli fructose repressor, FruR, is known to regulate expression of several genes concerned with carbon utilization. Using a previously derived consensus sequence for FruR binding, additional potential operators were identified and tested for FruR binding in DNA band migration retardation assays. Operato...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.1995.tb02339.x
更新日期：1995-06-01 00:00:00
abstract：:The cell walls of Sulfolobales species consist of proteinaceous S-layers assembled from two polypeptides, SlaA and SlaB. We isolated the large S-layer protein of Acidianus ambivalens and both S-layer subunits of Sulfolobus solfataricus and Metallosphaera sedula, respectively. The slaAB genes, lying adjacently in the c...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.2009.06746.x
更新日期：2009-07-01 00:00:00
abstract：:The transport of iron complexes through outer membrane transporters from Gram-negative bacteria is highly dependent on the TonB system. Together, the three components of the system, TonB, ExbB and ExbD, energize the transport of iron complexes through the outer membrane by utilizing the proton motive force across the ...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.2007.05957.x
更新日期：2007-11-01 00:00:00
abstract：:The nucleotide sequence of the traT gene present in the virulence-associated plasmid of Salmonella typhimurium was determined. The predicted TraT protein encoded by this gene was found to consist of 243 amino acids and to resemble the known TraT proteins of the plasmids of the F incompatibility group. Thus it contains...
journal_title：Molecular microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2958.1990.tb02014.x
更新日期：1990-01-01 00:00:00
