Abstract:
:The Escherichia coli fructose repressor, FruR, is known to regulate expression of several genes concerned with carbon utilization. Using a previously derived consensus sequence for FruR binding, additional potential operators were identified and tested for FruR binding in DNA band migration retardation assays. Operators in the control regions of operons concerned with carbon metabolism bound FruR, while those in operons not concerned with carbon metabolism did not. In vivo assays with transcriptional lacZ fusions showed that FruR controls the expression of FruR operator-containing genes encoding key enzymes of virtually every major pathway of carbon metabolism. Moreover, a fruR null mutation altered the rates of utilization of at least 36 carbon sources. In general, oxidation rates for glycolytic substances were enhanced while those for gluconeogenic substances were depressed. Alignment of FruR operators revealed that the consensus sequence for FruR binding is the same for operons that are activated and repressed by FruR and permitted formulation of a revised FruR-binding consensus sequence. The reported observations indicate that FruR modulates the direction of carbon flow by transcriptional activation of genes encoding enzymes concerned with oxidative and gluconeogenic carbon flow and by repression of those concerned with fermentative carbon flow.
journal_name
Mol Microbioljournal_title
Molecular microbiologyauthors
Ramseier TM,Bledig S,Michotey V,Feghali R,Saier MH Jrdoi
10.1111/j.1365-2958.1995.tb02339.xsubject
Has Abstractpub_date
1995-06-01 00:00:00pages
1157-69issue
6eissn
0950-382Xissn
1365-2958journal_volume
16pub_type
杂志文章abstract::To establish a lysogenic lifestyle, the temperate bacteriophage φC31 integrates its genome into the chromosome of its Streptomyces host, by site-specific recombination between attP (the attachment site in the phage DNA) and attB (the chromosomal attachment site). This reaction is promoted by a phage-encoded serine rec...
journal_title:Molecular microbiology
pub_type: 评论,杂志文章
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更新日期:2011-06-01 00:00:00
abstract::Fungal pathogens have evolved sophisticated machinery to precisely balance the fine line between acquiring essential metals and defending against metal toxicity. Iron and copper are essential metals for many processes in both fungal pathogens and their mammalian hosts, but reduce viability when present in excess. Howe...
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pub_type: 杂志文章,评审
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更新日期:2014-07-01 00:00:00
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pub_type: 杂志文章
doi:10.1111/j.1365-2958.2005.04667.x
更新日期:2005-07-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2958.2007.05823.x
更新日期:2007-08-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:2000-05-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2958.2005.04641.x
更新日期:2005-06-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2958.2004.04340.x
更新日期:2004-12-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2958.1997.4421810.x
更新日期:1997-07-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:1998-11-01 00:00:00
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pub_type: 杂志文章
doi:10.1111/j.1365-2958.2006.05553.x
更新日期:2007-02-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2958.1999.01457.x
更新日期:1999-06-01 00:00:00
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pub_type: 杂志文章
doi:10.1046/j.1365-2958.2001.02500.x
更新日期:2001-07-01 00:00:00
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pub_type: 杂志文章
doi:10.1111/j.1365-2958.1993.tb01955.x
更新日期:1993-10-01 00:00:00
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pub_type: 杂志文章
doi:10.1111/j.1365-2958.2010.07437.x
更新日期:2011-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1111/j.1365-2958.1996.tb02638.x
更新日期:1996-06-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2958.2010.07356.x
更新日期:2010-11-01 00:00:00
abstract::The replication origin of the broad-host-range plasmid RK2, oriV, contains four DnaA boxes, which bind the DnaA protein isolated from Escherichia coli. Using a transformation assay, mutational analysis of these boxes showed a differential requirement for replication in different Gram-negative bacteria. DnaA boxes 3 an...
journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2958.1999.01491.x
更新日期:1999-08-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2958.2010.07159.x
更新日期:2010-05-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2018-06-01 00:00:00
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pub_type: 杂志文章
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更新日期:2007-04-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:2002-07-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2958.1998.00968.x
更新日期:1998-07-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:2005-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:2006-12-01 00:00:00
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pub_type: 杂志文章
doi:10.1111/mmi.12799
更新日期:2014-11-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2958.1992.tb01777.x
更新日期:1992-11-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章,评审
doi:10.1111/mmi.12110
更新日期:2013-02-01 00:00:00
abstract::Recent studies examining the molecular mechanisms of isoniazid (INH) resistance in Mycobacterium tuberculosis have demonstrated that a significant percentage of drug-resistant strains are mutated in the katG gene which encodes a catalase-peroxidase, and the majority of these alterations are missense mutations which re...
journal_title:Molecular microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2958.1996.00133.x
更新日期:1996-11-01 00:00:00