Decorin-binding adhesins from Borrelia burgdorferi.


:Lyme disease is a tick-transmitted infection caused by the spirochete Borrelia burgdorferi. Ticks deposit B. burgdorferi into the dermis of the host, where they eventually become associated with collagen fibres. We demonstrated previously that B. burgdorferi is unable to bind collagen, but can bind the collagen-associated proteoglycan decorin and expresses decorin-binding proteins (Dbps). We have now cloned and sequenced two genes encoding the proteins, DbpA and DbpB, which have a similar structure, as revealed by circular dichroism (CD) spectroscopy of recombinant proteins. Competition experiments revealed a difference in binding specificity between DbpA and DbpB. Western blot analysis of proteinase K-treated intact B. burgdorferi and transmission electron microscopy studies using antibodies raised against recombinant Dbps demonstrated that these proteins are surface exposed. DbpA effectively inhibits the attachment of B. burgdorferi to a decorin substrate, whereas DbpB had a marginal effect, suggesting a difference in substrate specificity between the two Dbps. Polystyrene beads coated with DbpA adhered to a decorin-containing extracellular matrix produced by cultured skin fibroblasts, whereas beads coated with OspC did not. Taken together, these data suggest that Dbps are adhesins of the MSCRAMM (microbial surface component-recognizing adhesive matrix molecule) family, which mediate B. burgdorferi attachment to the extracellular matrix of the host.


Mol Microbiol


Molecular microbiology


Guo BP,Brown EL,Dorward DW,Rosenberg LC,Höök M




Has Abstract


1998-11-01 00:00:00












  • Selective expression of the beta-subunit of nucleoid-associated protein HU during cold shock in Escherichia coli.

    abstract::Expression of Escherichia coli hupA and hupB, the structural genes encoding the most abundant nucleoid-associated proteins HUalpha and HUbeta has been studied during cold shock. This article demonstrates that: (i) transcriptional expression of hupA is blocked following a sudden temperature downshift (from 37 degrees C...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Giangrossi M,Giuliodori AM,Gualerzi CO,Pon CL

    更新日期:2002-04-01 00:00:00

  • A tertiary structural element in S box leader RNAs is required for S-adenosylmethionine-directed transcription termination.

    abstract::The S box transcription termination control system regulates expression of genes involved in methionine metabolism. Expression of the S box regulon, comprised of 11 transcriptional units in Bacillus subtilis, is induced in response to starvation for methionine. We previously demonstrated that S-adenosylmethionine (SAM...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: McDaniel BA,Grundy FJ,Henkin TM

    更新日期:2005-08-01 00:00:00

  • Characterization of Cryptococcus neoformans variety gattii SOD2 reveals distinct roles of the two superoxide dismutases in fungal biology and virulence.

    abstract::We studied superoxide dismutases (SODs) in the encapsulated yeast Cryptococcus neoformans (Cn) variety gattii to analyse the role of mitochondrial MnSOD (SOD2) in fungal biology and virulence. SOD2 was cloned from a Cn cosmid library, sod2 mutant and sod2 + SOD2 reconstituted strains were constructed by homologous rec...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Narasipura SD,Chaturvedi V,Chaturvedi S

    更新日期:2005-03-01 00:00:00

  • Genetic and biochemical interactions between the bacterial replication initiator DnaA and the nucleoid-associated protein Rok in Bacillus subtilis.

    abstract::We identified interactions between the conserved bacterial replication initiator and transcription factor DnaA and the nucleoid-associated protein Rok of Bacillus subtilis. DnaA binds directly to clusters of DnaA boxes at the origin of replication and elsewhere, including the promoters of several DnaA-regulated genes....

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Seid CA,Smith JL,Grossman AD

    更新日期:2017-03-01 00:00:00

  • The Nudix hydrolases of Deinococcus radiodurans.

    abstract::All 21 of the Nudix hydrolase genes from the radiation-resistant organism Deinococcus radiodurans have been cloned into vectors under the control of T7 promoters and expressed as soluble proteins in Escherichia coli. Their sizes range from 9.8 kDa (91 amino acids) to 59 kDa (548 amino acids). Two novel proteins were i...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Xu W,Shen J,Dunn CA,Desai S,Bessman MJ

    更新日期:2001-01-01 00:00:00

  • An experimentalist's guide to computational modelling of the Min system.

    abstract::Spatio-temporal oscillations of the Min proteins are essential for selecting the cell division site in Escherichia coli. These oscillations are a key example of a biological phenomenon that can only be understood on a systems level rather than on the level of its individual components. Here, we review the key concepts...

    journal_title:Molecular microbiology

    pub_type: 杂志文章,评审


    authors: Kruse K,Howard M,Margolin W

    更新日期:2007-03-01 00:00:00

  • The role of a cytoplasmic loop of MotA in load-dependent assembly and disassembly dynamics of the MotA/B stator complex in the bacterial flagellar motor.

    abstract::The proton-driven flagellar motor of Salmonella enterica can accommodate a dozen MotA/B stators in a load-dependent manner. The C-terminal periplasmic domain of MotB acts as a structural switch to regulate the number of active stators in the motor in response to load change. The cytoplasmic loop termed MotAC is respon...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Pourjaberi SNS,Terahara N,Namba K,Minamino T

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  • Neisseria gonorrhoeae acquires mutations in analogous regions of gyrA and parC in fluoroquinolone-resistant isolates.

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    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Belland RJ,Morrison SG,Ison C,Huang WM

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  • Frequent interspecific genetic exchange between commensal Neisseriae and Neisseria meningitidis.

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    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Linz B,Schenker M,Zhu P,Achtman M

    更新日期:2000-06-01 00:00:00

  • Phosphorylation-mediated regulation of heat shock response in Escherichia coli.

    abstract::Escherichia coli has two heat shock regulons under the transcriptional control of Esigma32 and EsigmaE RNA polymerases. These polymerases control the expression of genes, the products of which are needed for correct folding of proteins in the cytoplasm and the extracytoplasm respectively. In this study, we report that...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Klein G,Dartigalongue C,Raina S

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  • DNA replication-coupled inactivation of DnaA protein in vitro: a role for DnaA arginine-334 of the AAA+ Box VIII motif in ATP hydrolysis.

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    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Su'etsugu M,Kawakami H,Kurokawa K,Kubota T,Takata M,Katayama T

    更新日期:2001-04-01 00:00:00

  • Temperature effect on inclusion body formation and stress response in the periplasm of Escherichia coli.

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    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Hunke S,Betton JM

    更新日期:2003-12-01 00:00:00

  • NAD+ binding to the Escherichia coli K(+)-uptake protein TrkA and sequence similarity between TrkA and domains of a family of dehydrogenases suggest a role for NAD+ in bacterial transport.

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    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Schlösser A,Hamann A,Bossemeyer D,Schneider E,Bakker EP

    更新日期:1993-08-01 00:00:00

  • Viral escape from antisense RNA.

    abstract::RNA coliphage SP was propagated for several generations on a host expressing an inhibitory antisense RNA complementary to bases 31-270 of the positive-stranded genome. Phages evolved that escaped inhibition. Typically, these escape mutants contained 3-4 base substitutions, but different sequences were observed among d...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Bull JJ,Jacobson A,Badgett MR,Molineux IJ

    更新日期:1998-05-01 00:00:00

  • Probing the structure-activity relationship of Escherichia coli LT-A by site-directed mutagenesis.

    abstract::Computer analysis of the crystallographic structure of the A subunit of Escherichia coli heat-labile toxin (LT) was used to predict residues involved in NAD binding, catalysis and toxicity. Following site-directed mutagenesis, the mutants obtained could be divided into three groups. The first group contained fully ass...

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    pub_type: 杂志文章


    authors: Pizza M,Domenighini M,Hol W,Giannelli V,Fontana MR,Giuliani MM,Magagnoli C,Peppoloni S,Manetti R,Rappuoli R

    更新日期:1994-10-01 00:00:00

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    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Hauser K,Pavlovic N,Klauke N,Geissinger D,Plattner H

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    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Escuyer V,Mock M

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  • Rhomboid proteases in invasion and replication of Apicomplexa.

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    journal_title:Molecular microbiology

    pub_type: 评论,杂志文章


    authors: Stewart RJ,Tonkin CJ

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  • Phospholipases C are involved in the virulence of Mycobacterium tuberculosis.

    abstract::Phospholipases C play a role in the pathogenesis of several bacteria. Mycobacterium tuberculosis, the causative agent of tuberculosis, possesses four genes encoding putative phospholipases C, plcA, plcB, plcC and plcD. However, the contribution of these genes to virulence is unknown. We constructed four single mutants...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Raynaud C,Guilhot C,Rauzier J,Bordat Y,Pelicic V,Manganelli R,Smith I,Gicquel B,Jackson M

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  • Selective inactivation of SIDER2 retroposon-mediated mRNA decay contributes to stage- and species-specific gene expression in Leishmania.

    abstract::Despite their high genomic synteny, the Leishmania major and Leishmania infantum species exhibit extensive differences in mRNA expression patterns throughout the parasite's development. Yet, the underlying mechanisms for this species-specific differential gene expression are largely unknown. Here we report that Short ...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Müller M,Padmanabhan PK,Papadopoulou B

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  • The aspartate chemoreceptor Tar is effectively methylated by binding to the methyltransferase mainly through hydrophobic interaction.

    abstract::In the chemotaxis of Escherichia coli, adaptation requires the methylation and demethylation of transmembrane receptors, which are catalysed by the methyltransferase CheR and the methylesterase CheB respectively. CheR binds to major chemoreceptors through their C-terminal motif NWETF, which is distinct from the methyl...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Shiomi D,Okumura H,Homma M,Kawagishi I

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  • Characterization of the dTDP-rhamnose biosynthetic genes encoded in the rfb locus of Shigella flexneri.

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    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Macpherson DF,Manning PA,Morona R

    更新日期:1994-01-01 00:00:00

  • Isolation and characterization of the secE homologue gene of Bacillus subtilis.

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    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Jeong SM,Yoshikawa H,Takahashi H

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    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Dempsey WB

    更新日期:1989-04-01 00:00:00

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    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Papenfort K,Pfeiffer V,Lucchini S,Sonawane A,Hinton JC,Vogel J

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  • A gene required for nuclear migration in Neurospora crassa codes for a protein with cysteine-rich, LIM/RING-like domains.

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    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Vierula PJ,Mais JM

    更新日期:1997-04-01 00:00:00

  • The Aspergillus nidulans metR gene encodes a bZIP protein which activates transcription of sulphur metabolism genes.

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    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Natorff R,Sieńko M,Brzywczy J,Paszewski A

    更新日期:2003-08-01 00:00:00

  • The Haemophilus influenzae Hia autotransporter harbours two adhesive pockets that reside in the passenger domain and recognize the same host cell receptor.

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    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Laarmann S,Cutter D,Juehne T,Barenkamp SJ,St Geme JW

    更新日期:2002-11-01 00:00:00

  • The pilE gene product of Pseudomonas aeruginosa, required for pilus biogenesis, shares amino acid sequence identity with the N-termini of type 4 prepilin proteins.

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    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Russell MA,Darzins A

    更新日期:1994-09-01 00:00:00

  • Characterization of mutations in the PAS domain of the EvgS sensor kinase selected by laboratory evolution for acid resistance in Escherichia coli.

    abstract::Laboratory-based evolution and whole-genome sequencing can link genotype and phenotype. We used evolution of acid resistance in exponential phase Escherichia coli to study resistance to a lethal stress. Iterative selection at pH 2.5 generated five populations that were resistant to low pH in early exponential phase. G...

    journal_title:Molecular microbiology

    pub_type: 杂志文章


    authors: Johnson MD,Bell J,Clarke K,Chandler R,Pathak P,Xia Y,Marshall RL,Weinstock GM,Loman NJ,Winn PJ,Lund PA

    更新日期:2014-09-01 00:00:00