Sulfur availability for Vibrio fischeri growth during symbiosis establishment depends on biogeography within the squid light organ.

Abstract:

:The fitness of host-associated microbes depends on their ability to access nutrients in vivo. Identifying these mechanisms is significant for understanding how microbes have evolved to fill specific ecological niches within a host. Vibrio fischeri is a bioluminescent bacterium that colonizes and proliferates within the light organ of the Hawaiian bobtail squid, which provides an opportunity to study how bacteria grow in vivo. Here, the transcription factor CysB is shown to be necessary for V. fischeri both to grow on several sulfur sources in vitro and to establish symbiosis with juvenile squid. CysB is also found to regulate several genes involved in sulfate assimilation and to contribute to the growth of V. fischeri on cystine, which is the oxidized form of cysteine. A mutant that grows on cystine but not sulfate could establish symbiosis, suggesting that V. fischeri acquires nutrients related to this compound within the host. Finally, CysB-regulated genes are shown to be differentially expressed among the V. fischeri populations occupying the various colonization sites found within the light organ. Together, these results suggest the biogeography of V. fischeri populations within the squid light organ impacts the physiology of this symbiotic bacterium in vivo through CysB-dependent gene regulation.

journal_name

Mol Microbiol

journal_title

Molecular microbiology

authors

Wasilko NP,Larios-Valencia J,Steingard CH,Nunez BM,Verma SC,Miyashiro T

doi

10.1111/mmi.14177

subject

Has Abstract

pub_date

2019-03-01 00:00:00

pages

621-636

issue

3

eissn

0950-382X

issn

1365-2958

journal_volume

111

pub_type

杂志文章
  • Peptide-mediated macrolide resistance reveals possible specific interactions in the nascent peptide exit tunnel.

    abstract::Expression of specific short peptides can render cells resistant to macrolide antibiotics. Peptides conferring resistance to structurally different macrolides including oleandomycin, azithromycin, azaerythromycin, josamycin and a ketolide cethromycin were selected from a random pentapeptide expression library. Analysi...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.2004.04290.x

    authors: Vimberg V,Xiong L,Bailey M,Tenson T,Mankin A

    更新日期:2004-10-01 00:00:00

  • Conversion of an extracellular cytolysin into a phagosome-specific lysin which supports the growth of an intracellular pathogen.

    abstract::Listeria monocytogenes is a facultative intracellular pathogen which secretes a pore-forming cytolysin, listeriolysin O (LLO), necessary for intracellular growth. Clostridium perfringens is an extracellular pathogen which secretes a related cytolysin, perfringolysin O (PFO). When PFO is secreted by intracellular L. mo...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2958.1996.00074.x

    authors: Jones S,Preiter K,Portnoy DA

    更新日期:1996-09-01 00:00:00

  • Control of translation by mRNA secondary structure: the importance of the kinetics of structure formation.

    abstract::RNA secondary structure is important in a wide variety of biological processes, but relatively little is known about the pathways and kinetics of RNA folding. When the IS10 transposase (tnp) gene is transcribed from a promoter outside the element, little increase in tnp expression is observed. This protection from out...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.1994.tb01337.x

    authors: Ma CK,Kolesnikow T,Rayner JC,Simons EL,Yim H,Simons RW

    更新日期:1994-12-01 00:00:00

  • The frequency of expression of pyelonephritis-associated pili is under regulatory control.

    abstract::The Escherichia coli urinary tract isolate C1212 contains two pyelonephritis-associated pili (pap) DNA sequences designated here as pap-17 and pap-21. Each of these pap sequences encodes antigenically-distinct pilin monomers, pilin-17 and pilin-21, respectively. Most individual strain C1212 cells isolated from a singl...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.1987.tb01940.x

    authors: Low D,Robinson EN Jr,McGee ZA,Falkow S

    更新日期:1987-11-01 00:00:00

  • A novel DNA modification by sulphur.

    abstract::Streptomyces lividans has a novel DNA modification, which sensitises its DNA to degradation during electrophoresis (the Dnd phenotype). The entire gene cluster (dnd) involved in this modification was localized on an 8 kb DNA fragment and was expressed in a S. lividans deletion mutant (dnd) and in several heterologous ...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.2005.04764.x

    authors: Zhou X,He X,Liang J,Li A,Xu T,Kieser T,Helmann JD,Deng Z

    更新日期:2005-09-01 00:00:00

  • Back to log phase: sigma S as a global regulator in the osmotic control of gene expression in Escherichia coli.

    abstract::It is now well established that the sigma S subunit of RNA polymerase is a master regulator in a complex regulatory network that governs the expression of many stationary-phase-inducible genes in Escherichia coli. In this review, more recent findings will be summarized that demonstrate that sigma S also acts as a glob...

    journal_title:Molecular microbiology

    pub_type: 杂志文章,评审

    doi:10.1046/j.1365-2958.1996.511405.x

    authors: Hengge-Aronis R

    更新日期:1996-09-01 00:00:00

  • New erythromycin derivatives from Saccharopolyspora erythraea using sugar O-methyltransferases from the spinosyn biosynthetic gene cluster.

    abstract::Using a previously developed expression system based on the erythromycin-producing strain of Saccharopolyspora erythraea, O-methyltransferases from the spinosyn biosynthetic gene cluster of Saccharopolyspora spinosa have been shown to modify a rhamnosyl sugar attached to a 14-membered polyketide macrolactone. The spnI...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2958.2001.02594.x

    authors: Gaisser S,Lill R,Wirtz G,Grolle F,Staunton J,Leadlay PF

    更新日期:2001-09-01 00:00:00

  • Families of bacterial signal-transducing proteins.

    abstract::Bacteria can respond to a variety of environmental stimuli by means of systems generally composed of two proteins. The first protein (sensor or transmitter) is usually a transmembrane protein with cytoplasmic and extracytoplasmic domains. The extracytoplasmic domain (sensor) senses the environment and transfers the si...

    journal_title:Molecular microbiology

    pub_type: 杂志文章,评审

    doi:10.1111/j.1365-2958.1989.tb00152.x

    authors: Gross R,Aricò B,Rappuoli R

    更新日期:1989-11-01 00:00:00

  • Distinct functions of serial metal-binding domains in the Escherichia coli P1 B -ATPase CopA.

    abstract::P1 B -ATPases are among the most common resistance factors to metal-induced stress. Belonging to the superfamily of P-type ATPases, they are capable of exporting transition metal ions at the expense of adenosine triphosphate (ATP) hydrolysis. P1 B -ATPases share a conserved structure of three cytoplasmic domains linke...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/mmi.13038

    authors: Drees SL,Beyer DF,Lenders-Lomscher C,Lübben M

    更新日期:2015-08-01 00:00:00

  • IS231D, E and F, three new insertion sequences in Bacillus thuringiensis: extension of the IS231 family.

    abstract::IS231 constitutes a family of insertion sequences widespread among Bacillus thuringiensis subspecies. Three new IS231 variants have been isolated from B. thuringiensis subspecies finitimus (IS231 D and E) and israelensis (IS231F). Like the previously described IS231A, B and C, these 1.7 kb elements display single open...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.1992.tb01369.x

    authors: Rezsöhazy R,Hallet B,Delcour J

    更新日期:1992-07-01 00:00:00

  • Chromosomal constraints in Gram-positive bacteria revealed by artificial inversions.

    abstract::We used artificial chromosome inversions to investigate the chromosomal constraints that preserve genome organization in the Gram-positive bacterium Lactococcus lactis. Large inversions, 80-1260 kb in length, disturbing the symmetry of the origin and terminus of the replication axis to various extents, were constructe...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2958.2003.03847.x

    authors: Campo N,Dias MJ,Daveran-Mingot ML,Ritzenthaler P,Le Bourgeois P

    更新日期:2004-01-01 00:00:00

  • Cloning and sequence analysis of linear plasmid telomeres of the bacterium Borrelia burgdorferi.

    abstract::Borrelia burgdorferi, the Lyme disease agent, has double-stranded linear plasmids with covalently closed ends. DNA at the ends, or telomeres, of two linear plasmids of B. burgdorferi strain B31 was examined. Telomeric sequences from both ends of a 16 kb linear plasmid and from one end of a 49 kb linear plasmid were cl...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.1990.tb00651.x

    authors: Hinnebusch J,Bergström S,Barbour AG

    更新日期:1990-05-01 00:00:00

  • Salmonella effectors translocated across the vacuolar membrane interact with the actin cytoskeleton.

    abstract::A family of nine Salmonella typhimurium type III secretion effectors with a conserved amino-terminus have been defined. Three family members (SifA, SifB and SseJ) have previously been demonstrated to localize to the Salmonella-containing vacuole and to Salmonella-induced filaments. In contrast, we demonstrate that two...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2958.2003.t01-1-03456.x

    authors: Miao EA,Brittnacher M,Haraga A,Jeng RL,Welch MD,Miller SI

    更新日期:2003-04-01 00:00:00

  • The bacterial signal transduction protein GlnB regulates the committed step in fatty acid biosynthesis by acting as a dissociable regulatory subunit of acetyl-CoA carboxylase.

    abstract::Biosynthesis of fatty acids is one of the most fundamental biochemical pathways in nature. In bacteria and plant chloroplasts, the committed and rate-limiting step in fatty acid biosynthesis is catalyzed by a multi-subunit form of the acetyl-CoA carboxylase enzyme (ACC). This enzyme carboxylates acetyl-CoA to produce ...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/mmi.12912

    authors: Gerhardt EC,Rodrigues TE,Müller-Santos M,Pedrosa FO,Souza EM,Forchhammer K,Huergo LF

    更新日期:2015-03-01 00:00:00

  • Identification of the core transmembrane complex of the Legionella Dot/Icm type IV secretion system.

    abstract::Type IV secretion systems (T4SS) are utilized by a wide range of Gram negative bacteria to deliver protein and DNA substrates to recipient cells. The best characterized T4SS are the type IVA systems, which exhibit extensive similarity to the Agrobacterium VirB T4SS. In contrast, type IVB secretion systems share almost...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.2006.05446.x

    authors: Vincent CD,Friedman JR,Jeong KC,Buford EC,Miller JL,Vogel JP

    更新日期:2006-12-01 00:00:00

  • Specific and pleiotropic patterns of mRNA regulation by ArcZ, a conserved, Hfq-dependent small RNA.

    abstract::The small RNA, ArcZ (previously RyhA/SraH), was discovered in several genome-wide screens in Escherichia coli and Salmonella. Its high degree of genomic conservation, its frequent recovery by shotgun sequencing, and its association with the RNA chaperone, Hfq, identified ArcZ as an abundant enterobacterial 'core' smal...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.2009.06857.x

    authors: Papenfort K,Said N,Welsink T,Lucchini S,Hinton JCD,Vogel J

    更新日期:2009-10-01 00:00:00

  • The cell biology of secondary endosymbiosis--how parasites build, divide and segregate the apicoplast.

    abstract::Protozoan parasites of the phylum Apicomplexa harbour a chloroplast-like organelle, the apicoplast. The biosynthetic pathways localized to this organelle are of cyanobacterial origin and therefore offer attractive targets for the development of new drugs for the treatment of malaria and toxoplasmosis. The apicoplast a...

    journal_title:Molecular microbiology

    pub_type: 杂志文章,评审

    doi:10.1111/j.1365-2958.2006.05343.x

    authors: Vaishnava S,Striepen B

    更新日期:2006-09-01 00:00:00

  • A suf operon requirement for Fe-S cluster assembly during iron starvation in Escherichia coli.

    abstract::The suf and isc operons of Escherichia coli have been implicated in Fe-S cluster assembly. However, it has been unclear why E. coli has two systems for Fe-S cluster biosynthesis. We have examined the regulatory characteristics and mutant phenotypes of both operons to discern if the two operons have redundant functions...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.2004.04025.x

    authors: Outten FW,Djaman O,Storz G

    更新日期:2004-05-01 00:00:00

  • Genetic analysis of Agrobacterium tumefaciens unipolar polysaccharide production reveals complex integrated control of the motile-to-sessile switch.

    abstract::Many bacteria colonize surfaces and transition to a sessile mode of growth. The plant pathogen Agrobacterium tumefaciens produces a unipolar polysaccharide (UPP) adhesin at single cell poles that contact surfaces. Here we report that elevated levels of the intracellular signal cyclic diguanosine monophosphate (c-di-GM...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/mmi.12321

    authors: Xu J,Kim J,Koestler BJ,Choi JH,Waters CM,Fuqua C

    更新日期:2013-09-01 00:00:00

  • Unique roles of the rrn P2 rRNA promoters in Escherichia coli.

    abstract::In bacteria, genes are often expressed from multiple promoters to allow for a greater spectrum of regulation. Transcription of rRNA genes in Escherichia coli uses two promoters, rrn P1 and rrn P2. Under the conditions examined previously, the P1 and P2 promoters were regulated in response to many of the same changes i...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.2004.04060.x

    authors: Murray HD,Gourse RL

    更新日期:2004-06-01 00:00:00

  • The essential roles of cytidine diphosphate-diacylglycerol synthase in bloodstream form Trypanosoma brucei.

    abstract::Lipid metabolism in Trypanosoma brucei, the causative agent of African sleeping sickness, differs from its human host in several fundamental ways. This has lead to the validation of a plethora of novel drug targets, giving hope of novel chemical intervention against this neglected disease. Cytidine diphosphate diacylg...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/mmi.12553

    authors: Lilley AC,Major L,Young S,Stark MJ,Smith TK

    更新日期:2014-05-01 00:00:00

  • Recently differentiated epimastigotes from Trypanosoma cruzi are infective to the mammalian host.

    abstract::Trypanosoma cruzi, the etiologic agent of Chagas disease, has a complex life cycle in which four distinct developmental forms alternate between the insect vector and the mammalian host. It is assumed that replicating epimastigotes present in the insect gut are not infective to mammalian host, a paradigm corroborated b...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/mmi.13653

    authors: Kessler RL,Contreras VT,Marliére NP,Aparecida Guarneri A,Villamizar Silva LH,Mazzarotto GACA,Batista M,Soccol VT,Krieger MA,Probst CM

    更新日期:2017-06-01 00:00:00

  • Inactivation of the FNR protein of Escherichia coli by targeted mutagenesis in the N-terminal region.

    abstract::The FNR protein of Escherichia coli is a regulatory protein that activates the transcription of its target genes in response to oxygen limitation. Site-directed mutagenesis was used to show that a 28-residue N-terminal segment containing three cysteines is essential for normal FNR function. The cysteine residue which ...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.1988.tb00080.x

    authors: Spiro S,Guest JR

    更新日期:1988-11-01 00:00:00

  • Multiple stress signal integration in the regulation of the complex sigma S-dependent csiD-ygaF-gabDTP operon in Escherichia coli.

    abstract::The csiD-ygaF-gabDTP region in the Escherichia coli genome represents a cluster of sigma S-controlled genes. Here, we investigated promoter structures, sigma factor dependencies, potential co-regulation and environmental regulatory patterns for all of these genes. We find that this region constitutes a complex operon ...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2958.2003.03867.x

    authors: Metzner M,Germer J,Hengge R

    更新日期:2004-02-01 00:00:00

  • Characterization of integration host factor (IHF) binding upstream of the cysteine-rich protein operon (omcAB) promoter of Chlamydia trachomatis LGV serovar L2.

    abstract::Chlamydiae are bacterial parasites that carry out a distinct developmental cycle within host cells; however, the mechanisms by which these organisms regulate stage-specific gene expression are not known. We identified a DNA element located between nucleotide (nt) -135 and -90 upstream from the transcription start poin...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2958.2001.02531.x

    authors: Zhong J,Douglas AL,Hatch TP

    更新日期:2001-07-01 00:00:00

  • Characterization of a replicon of the moderately promiscuous plasmid, pGSH5000, with features of both the mini-replicon of pCU1 and the ori-2 of F.

    abstract::The dominant, polA1-independent replicon of pGSH500, rep beta (1.8 kb), consists of a cis-acting oriV region of 245 bp; a repB gene that is essential for autonomous replication and 18, 30 to 36 bp iterons which constitute the inc/cop region. The molecular organization of rep beta resembles that of mini-pCU1 (IncN). Fu...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.1993.tb01171.x

    authors: da Silva-Tatley FM,Steyn LM

    更新日期:1993-03-01 00:00:00

  • The FinO protein of IncF plasmids binds FinP antisense RNA and its target, traJ mRNA, and promotes duplex formation.

    abstract::Most of the genes required for the conjugative transfer of DNA are encoded by the 33 kb transfer (tra) operon of F-like conjugative plasmids. Transcription of the tra operon is positively regulated by the TraJ transcriptional activator which, in turn, is negatively regulated by the FinOP fertility inhibition system. T...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.1994.tb02177.x

    authors: van Biesen T,Frost LS

    更新日期:1994-11-01 00:00:00

  • Cold-regulated genes under control of the cold sensor Hik33 in Synechocystis.

    abstract::A histidine kinase, Hik33, appears to sense decreases in temperature and to regulate the expression of certain cold-inducible genes in the cyanobacterium Synechocystis sp. PCC6803. To examine the role of Hik33 in the regulation of gene expression, we analysed a DeltaHik33 mutant using the DNA microarray technique. In ...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2958.2001.02379.x

    authors: Suzuki I,Kanesaki Y,Mikami K,Kanehisa M,Murata N

    更新日期:2001-04-01 00:00:00

  • Differential regulation of antibiotic biosynthesis by DraR-K, a novel two-component system in Streptomyces coelicolor.

    abstract::A novel two-component system (TCS) designated as DraR-K (sco3063/sco3062) was identified to be involved in differential regulation of antibiotic biosynthesis in Streptomyces coelicolor. The S. coelicolor mutants with deletion of either or both of draR and draK exhibited significantly reduced actinorhodin (ACT) but inc...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.2012.08126.x

    authors: Yu Z,Zhu H,Dang F,Zhang W,Qin Z,Yang S,Tan H,Lu Y,Jiang W

    更新日期:2012-08-01 00:00:00

  • Hysteretic and graded responses in bacterial two-component signal transduction.

    abstract::Bacterial two-component systems (TCS) are key signal transduction networks regulating global responses to environmental change. Environmental signals may modulate the phosphorylation state of sensor kinases (SK). The phosphorylated SK transfers the phosphate to its cognate response regulator (RR), which causes physiol...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.2008.06221.x

    authors: Igoshin OA,Alves R,Savageau MA

    更新日期:2008-06-01 00:00:00