Abstract:
:pAM beta 1 is a low-copy-number, promiscuous plasmid from Gram-positive bacteria that replicates by a unidirectional theta-type mode. Its replication is initiated by an original mechanism, involving the positive rate-limiting RepE protein. Here we show that the pAM beta 1-encoded CopF protein is involved in negative regulation of the plasmid copy number. CopF represses approximately 10-fold the transcription initiated at the promoter of the repE gene and binds to a 31 bp segment which is located immediately upstream of the -35 box of the repE promoter. We propose that CopF inhibits initiation of transcription at the repE promoter by binding to its operator.
journal_name
Mol Microbioljournal_title
Molecular microbiologyauthors
Le Chatelier E,Ehrlich SD,Jannière Ldoi
10.1111/j.1365-2958.1994.tb02181.xsubject
Has Abstractpub_date
1994-11-01 00:00:00pages
463-71issue
3eissn
0950-382Xissn
1365-2958journal_volume
14pub_type
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