Deletion analysis resolves cell-binding and lytic domains of the Pasteurella leukotoxin.

abstract：:Mismatch repair (MMR) corrects DNA polymerase errors occurring during genome replication. MMR is critical for genome maintenance, and its loss increases mutation rates several hundred fold. Recent work has shown that the interaction between the mismatch recognition protein MutS and the replication processivity clamp i...

journal_title：Molecular microbiology

authors： Klocko AD,Schroeder JW,Walsh BW,Lenhart JS,Evans ML,Simmons LA

更新日期：2011-11-01 00:00:00

abstract：:We present structural and biochemical evidence for a redox switch in the archaeal transcriptional regulator SurR of Pyrococcus furiosus, a hyperthermophilic anaerobe. P. furiosus produces H(2) during fermentation, but undergoes a metabolic shift to produce H(2) S when elemental sulfur (S(0) ) becomes available. Change...

journal_title：Molecular microbiology

authors： Yang H,Lipscomb GL,Keese AM,Schut GJ,Thomm M,Adams MW,Wang BC,Scott RA

更新日期：2010-09-01 00:00:00

abstract：:Lyme disease is a tick-transmitted infection caused by the spirochete Borrelia burgdorferi. Ticks deposit B. burgdorferi into the dermis of the host, where they eventually become associated with collagen fibres. We demonstrated previously that B. burgdorferi is unable to bind collagen, but can bind the collagen-associ...

journal_title：Molecular microbiology

authors： Guo BP,Brown EL,Dorward DW,Rosenberg LC,Höök M

更新日期：1998-11-01 00:00:00

abstract：:Virion proteins recognize their cognate nucleic acid for encapsidation into virions through recognition of a specific nucleotide sequence contained within that nucleic acid. Viruses like bacteriophage P22, which have partially circularly permuted, double-stranded virion DNAs, encapsidate DNA through processive series ...

journal_title：Molecular microbiology

authors： Wu H,Sampson L,Parr R,Casjens S

更新日期：2002-09-01 00:00:00

abstract：:The type-VII ESX-1 secretion apparatus, encoded by the esx-1 genetic locus, is essential for the export of EsxA and EsxB, two major virulence factors of Mycobacterium tuberculosis. ESX-1 also requires the products of the unlinked espACD operon for optimal function and these proteins are considered integral parts of th...

journal_title：Molecular microbiology

authors： Chen JM,Zhang M,Rybniker J,Boy-Röttger S,Dhar N,Pojer F,Cole ST

更新日期：2013-09-01 00:00:00

abstract：:Visual inspection showed clear evidence of a history of intraspecies recombinational exchanges within the neighbouring meningococcal shikimate dehydrogenase (aroE) and glutamine synthetase (glnA) genes, which was supported by the non-congruence of the trees constructed from the sequences of these genes from different ...

journal_title：Molecular microbiology

authors： Zhou J,Bowler LD,Spratt BG

更新日期：1997-02-01 00:00:00

abstract：:Photorhabdus are bacteria found colonizing the gut of a specialized stage of the nematode Heterorhabditis, called the infective juvenile (IJ). The IJ is a free-living stage of the nematode that seeks out and infects insect larvae. Once inside the insect the IJ release Photorhabdus into the haemolymph where the bacteri...

journal_title：Molecular microbiology

authors： Watson RJ,Joyce SA,Spencer GV,Clarke DJ

更新日期：2005-05-01 00:00:00

abstract：:Ferrichrome-iron is actively transported across the outer membrane of Escherichia coli by the TonB-dependent receptor FhuA. To obtain FhuA in a form suitable for secondary-structure analyses, a hexahistidine tag was inserted into a surface-located site and the recombinant protein was purified by metal chelate chromato...

journal_title：Molecular microbiology

authors： Moeck GS,Tawa P,Xiang H,Ismail AA,Turnbull JL,Coulton JW

更新日期：1996-11-01 00:00:00

abstract：:It has previously been proposed that Escherichia coli ribosomal protein S1 is required for the translation of highly structured mRNAs. In this study, we have examined the influence of structural features at or near the start codon of different mRNAs. The requirement for ribosomal protein S1 for translation initiation ...

journal_title：Molecular microbiology

authors： Tedin K,Resch A,Bläsi U

更新日期：1997-07-01 00:00:00

abstract：:Bacteria differ from eukaryotes by having the enzyme DNA gyrase, which catalyses the ATP-dependent negative supercoiling of DNA. Negative supercoils are essential for condensing chromosomes into an interwound (plectonemic) and branched structure known as the nucleoid. Topo-1 removes excess supercoiling in an ATP-indep...

journal_title：Molecular microbiology

authors： Booker BM,Deng S,Higgins NP

更新日期：2010-12-01 00:00:00

abstract：:The esg locus is required for the formation of multicellular fruiting bodies and spores by the developmental bacterium Myxococcus xanthus. Studies have suggested that esg mutants are defective in the production of an essential signal (E-signal) used in cell-cell communication and that E-signalling is required for the ...

journal_title：Molecular microbiology

authors： Downard J,Toal D

更新日期：1995-04-01 00:00:00

abstract：:Listeria monocytogenes is a facultative intracellular pathogen which secretes a pore-forming cytolysin, listeriolysin O (LLO), necessary for intracellular growth. Clostridium perfringens is an extracellular pathogen which secretes a related cytolysin, perfringolysin O (PFO). When PFO is secreted by intracellular L. mo...

journal_title：Molecular microbiology

authors： Jones S,Preiter K,Portnoy DA

更新日期：1996-09-01 00:00:00

abstract：:Proteus mirabilis is a common cause of urinary tract infection (UTI) in individuals with structural abnormalities or long-term catheterization. The expression of mannose-resistant/Proteus-like (MR/P) fimbria is phase variable because of the inversion of a 251 bp DNA fragment that carries the promoter for the mrp opero...

journal_title：Molecular microbiology

authors： Li X,Lockatell CV,Johnson DE,Mobley HL

更新日期：2002-08-01 00:00:00

abstract：:Three distinct types of terminal oxidases participate in the aerobic respiratory pathways of Paracoccus denitrificans. Two alternative genes encoding subunit I of the aa3-type cytochrome c oxidase have been isolated before, namely ctaDI and ctaDII. Each of these genes can be expressed separately to complement a double...

journal_title：Molecular microbiology

authors： de Gier JW,Lübben M,Reijnders WN,Tipker CA,Slotboom DJ,van Spanning RJ,Stouthamer AH,van der Oost J

更新日期：1994-07-01 00:00:00

abstract：:Mycobacterium tuberculosis infects one-third of the world's population and causes two million deaths annually. The unusually low permeability of its cell wall contributes to the ability of M. tuberculosis to grow within host macrophages, a property required for pathogenesis of infection. Mycobacterium marinum is an es...

journal_title：Molecular microbiology

authors： Gao LY,Laval F,Lawson EH,Groger RK,Woodruff A,Morisaki JH,Cox JS,Daffe M,Brown EJ

更新日期：2003-09-01 00:00:00

abstract：:The gene encoding a Lon protease homologue has been cloned from Brucella abortus. The putative Brucella abortus Lon shares > 60% amino acid identity with its Escherichia coli counterpart and the recombinant form of this protein restores the capacity of an Escherichia coli lon mutant to resist killing by ultraviolet ir...

journal_title：Molecular microbiology

authors： Robertson GT,Kovach ME,Allen CA,Ficht TA,Roop RM 2nd

更新日期：2000-02-01 00:00:00

abstract：:Mutants (glk) of Streptomyces coelicolor A3(2) that are resistant to the non-utilizable glucose analogue 2-deoxyglucose are deficient in glucose kinase activity, defective in glucose repression, and usually unable to utilize glucose. A 2.9 kb BclI fragment, previously shown to restore a wild-type phenotype to a glk de...

journal_title：Molecular microbiology

authors： Angell S,Schwarz E,Bibb MJ

更新日期：1992-10-01 00:00:00

abstract：:We have cloned a gene encoding a polygalacturonase (PG) in the filamentous fungus Aspergillus niger RH5344. The structural gene comprises 1141 bp coding for 362 amino acids and the open reading frame is disrupted by one intron of 52 bp. Eukaryotic consensus sequences for transcription regulation are found only in devi...

journal_title：Molecular microbiology

authors： Ruttkowski E,Khanh NQ,Wientjes FJ,Gottschalk M

更新日期：1991-06-01 00:00:00

abstract：:Replication fork arrest can cause DNA double-strand breaks (DSBs). These DSBs are caused by the action of the Holliday junction resolvase RuvABC, indicating that they are made by resolution of Holliday junctions formed at blocked forks. In this work, we study the homologous recombination functions required for RuvABC-...

journal_title：Molecular microbiology

authors： Seigneur M,Ehrlich SD,Michel B

更新日期：2000-11-01 00:00:00

abstract：:citS and citT genes encoding a new two-component system were identified in the 71 degrees region between the pel and citM loci on the Bacillus subtilis chromosome. citS- and citT-deficient strains were unable to grow on minimal plates including citrate as a sole carbon source. In addition, a strain deficient in citM, ...

journal_title：Molecular microbiology

authors： Yamamoto H,Murata M,Sekiguchi J

更新日期：2000-08-01 00:00:00

abstract：:There is a DNA sequence which has unusually high affinity for the DnaA protein of Escherichia coli between the glyV and amiB-mutL operons at 94.7 min on the genetic map. Affinity of DnaA protein for DNA was measured in vivo as the activity of beta-galactosidase produced from the lacZ gene on a plasmid fused to the 5'-...

journal_title：Molecular microbiology

authors： Kitagawa R,Mitsuki H,Okazaki T,Ogawa T

更新日期：1996-03-01 00:00:00

abstract：:Expression of the Saccharomyces cerevisiae nuclear gene CYB2 encoding the mitochondrial enzyme L-(+)-lactate-cytochrome c oxidoreductase (EC 1.2.2.3) is subject to several strict metabolic controls at the transcriptional level: repression due to glucose fermentation, derepression by ethanol, induction by lactate and i...

journal_title：Molecular microbiology

authors： Ramil E,Agrimonti C,Shechter E,Gervais M,Guiard B

更新日期：2000-09-01 00:00:00

abstract：:The increase in bacterial resistance to multiple drugs represents a serious and growing health risk. One component of multidrug resistance (MDR) is a group of multidrug transporters that are often regulated at the transcriptional level by repressors and/or activators. Some of these transcription factors are also multi...

journal_title：Molecular microbiology

authors： Schumacher MA,Brennan RG

更新日期：2002-08-01 00:00:00

abstract：:The pathogenicity of Plasmodium falciparum is partly due to parasite-induced host cell modifications. These modifications are facilitated by exported P. falciparum proteins, collectively referred to as the exportome. Export of several hundred proteins is mediated by the PEXEL/HT, a protease cleavage site. The PEXEL/HT...

journal_title：Molecular microbiology

authors： Schulze J,Kwiatkowski M,Borner J,Schlüter H,Bruchhaus I,Burmester T,Spielmann T,Pick C

更新日期：2015-07-01 00:00:00

abstract：:The pathogen Vibrio cholerae modulates the expression of many genes in order to transition from its environmental reservoir to its niche in the human host. Among these are genes encoding two related Type IV pili, the mannose-sensitive haemagglutinin (MSHA) pilus, which aids V. cholerae persistence in aquatic environme...

journal_title：Molecular microbiology

authors： Hsiao A,Toscano K,Zhu J

更新日期：2008-02-01 00:00:00

abstract：:Post-translational proteolysis-dependent regulation of critical cellular processes is a common feature in bacteria. The Escherichia coli Lon protease is involved in the control of the SOS response, acid tolerance and nutritional deprivation. Moreover, Lon plays a role in the regulation of toxin-antitoxin (TA) systems ...

journal_title：Molecular microbiology

authors： Langklotz S,Narberhaus F

更新日期：2011-06-01 00:00:00

abstract：:In the mouse model for systemic infection, natural a/alpha strains of C. albicans are more virulent and more competitive than their spontaneous MTL-homozygous offspring, which arise primarily by loss of one chromosome 5 homologue followed by duplication of the retained homologue (uniparental disomy). Deletion of eithe...

journal_title：Molecular microbiology

authors： Wu W,Lockhart SR,Pujol C,Srikantha T,Soll DR

更新日期：2007-06-01 00:00:00

abstract：:A model for the topology of the PhoE porin has been proposed according to which the polypeptide traverses the outer membrane sixteen times mostly as amphipathic beta-sheets, thereby exposing eight loops at the cell surface. Until now, no evidence has been obtained for the surface exposure of the third loop. Recently, ...

journal_title：Molecular microbiology

authors： Struyvé M,Visser J,Adriaanse H,Benz R,Tommassen J

更新日期：1993-01-01 00:00:00

abstract：:Expression of the gene pcnB, encoding the dispensable Escherichia coli poly(A) polymerase (PAPI), which is toxic when overproduced, was investigated. Its promoter was identified and found to be moderately strong when used to express a beta-galactosidase reporter. Expression levels were not affected by increasing or de...

journal_title：Molecular microbiology

authors： Binns N,Masters M

更新日期：2002-06-01 00:00:00

abstract：:Rifampicin resistance, a defining attribute of multidrug-resistant tuberculosis, is conferred by mutations in the β subunit of RNA polymerase. Sequencing of rifampicin-resistant (RIF-R) clinical isolates of Mycobacterium tuberculosis revealed, in addition to RIF-R mutations, enrichment of potential compensatory mutati...

journal_title：Molecular microbiology

authors： Song T,Park Y,Shamputa IC,Seo S,Lee SY,Jeon HS,Choi H,Lee M,Glynne RJ,Barnes SW,Walker JR,Batalov S,Yusim K,Feng S,Tung CS,Theiler J,Via LE,Boshoff HI,Murakami KS,Korber B,Barry CE 3rd,Cho SN

更新日期：2014-03-01 00:00:00