Abstract:
:Replication fork arrest can cause DNA double-strand breaks (DSBs). These DSBs are caused by the action of the Holliday junction resolvase RuvABC, indicating that they are made by resolution of Holliday junctions formed at blocked forks. In this work, we study the homologous recombination functions required for RuvABC-mediated breakage in cells deficient for the accessory replicative helicase Rep or deficient for the main Escherichia coli replicative helicase DnaB. We show that, in the rep mutant, RuvABC-mediated breakage occurs in the absence of the homologous recombination protein RecA. In contrast, in dnaBts mutants, most of the RuvABC-mediated breakage depends on the presence of RecA, which suggests that RecA participates in the formation of Holliday junctions at forks blocked by the inactivation of DnaB. This action of RecA does not involve the induction of the SOS response and does not require any of the recombination proteins essential for the presynaptic step of homologous recombination, RecBCD, RecF or RecO. Consequently, our observations suggest a new function for RecA at blocked replication forks, and we propose that RecA acts by promoting homologous recombination without the assistance of known presynaptic proteins.
journal_name
Mol Microbioljournal_title
Molecular microbiologyauthors
Seigneur M,Ehrlich SD,Michel Bdoi
10.1046/j.1365-2958.2000.02152.xsubject
Has Abstractpub_date
2000-11-01 00:00:00pages
565-74issue
3eissn
0950-382Xissn
1365-2958pii
mmi2152journal_volume
38pub_type
杂志文章abstract::Bactericidal/permeability-increasing protein (BPI) of neutrophils is a lipopolysaccharide (LPS)-binding antibacterial protein with specificity for Gram negative bacteria. BPI binding to the bacterial surface rapidly triggers potentially reversible bacterial growth inhibition and alterations of the outer membrane and, ...
journal_title:Molecular microbiology
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更新日期:1993-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:2011-06-01 00:00:00
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更新日期:2003-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:2009-12-01 00:00:00
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journal_title:Molecular microbiology
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doi:10.1111/mmi.12336
更新日期:2013-09-01 00:00:00
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更新日期:2003-10-01 00:00:00
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journal_title:Molecular microbiology
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doi:10.1111/j.1365-2958.1993.tb01660.x
更新日期:1993-06-01 00:00:00
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journal_title:Molecular microbiology
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更新日期:2016-06-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章,已发布勘误,评审
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更新日期:2019-09-01 00:00:00
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更新日期:2005-09-01 00:00:00
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journal_title:Molecular microbiology
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更新日期:2002-12-01 00:00:00
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journal_title:Molecular microbiology
pub_type: 杂志文章
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更新日期:2000-06-01 00:00:00
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journal_title:Molecular microbiology
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更新日期:1994-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:2000-09-01 00:00:00
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更新日期:1997-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:1997-05-01 00:00:00
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更新日期:1991-04-01 00:00:00
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更新日期:2020-04-01 00:00:00